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The map label for this gene is ecoRIM [H]

Identifier: 213692078

GI number: 213692078

Start: 1381364

End: 1382044

Strand: Direct

Name: ecoRIM [H]

Synonym: Blon_1197

Alternate gene names: 213692078

Gene position: 1381364-1382044 (Clockwise)

Preceding gene: 213692074

Following gene: 213692082

Centisome position: 48.76

GC content: 65.49

Gene sequence:

>681_bases
ATGGCATGTCGACAACGACGGCCGCGGGGGGGGTATTCGATCGCGGACCTGGCCGCCCGCCCATTGGACGGCGACGGCTC
CTTCGACTCGCCCGAATGCGAACACCTGCTCGACCGGCCCGGCGCGATCGTCGTCACCAACCCGCCGTTCAGCCACGCCA
TCCGGTTCATGCGCATGCTCCGCCGGCATCCCGCGACCGACTACCTGATCGTCGCCAACCTGAACCTCACCACCGCCAAC
GACGTATTCCCCATGATCAAGGAGGGACGCTGCCTCGTCGGCCTGAGCATCCACTCGGGCAGCATGTTCTTCCGACTGCC
CGACGACCGACCGAAGACGGGAAGCATGATCCGGCCCGACGGCACGGTCGGCGTCAACAGCATCCGATGGCTCACCAGCC
TCGCCGCCGCACGCGCCGACAAGACCTGGCCTCCCACGGGACACACGTACCGGGGACATGAGGACGAGTATCCGGAATAT
GATGCCTACGACGCGATCAACGTGGACTCCATGCGCATGATGCCCGACGATCACGACGGGCCGATGGGCGTCCCGTTGAA
CTTCCTCGAACATTGGGCGCCCGGCAACGGGTTCATGCTGCTCGACAACCTCGACGACCCGACGATCGACGGACGTCGCC
TGTACAAGCGACTGCTCGTCAGACGGACGCGGGACGCATGA

Upstream 100 bases:

>100_bases
CGCTCGACCACATGAACGAATACGGAATCGCGTCCCTCACCTGCACGAGCTTCCAACCCGACCATGGCACCCTGTTCGAC
GACGGGACGCCCGCCATGCA

Downstream 100 bases:

>100_bases
TCCACGCCGCCCGCGCATGCAGGCGGAACCCCGTGGGATAAACCGCCGTCCACGAACAGCGTGATACGAATCTTGTTGAA
GGACGAACGGGACGCCGGCC

Product: adenine-specific methyltransferase EcoRI

Products: NA

Alternate protein names: M.EcoRI; Adenine-specific methyltransferase EcoRI [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MACRQRRPRGGYSIADLAARPLDGDGSFDSPECEHLLDRPGAIVVTNPPFSHAIRFMRMLRRHPATDYLIVANLNLTTAN
DVFPMIKEGRCLVGLSIHSGSMFFRLPDDRPKTGSMIRPDGTVGVNSIRWLTSLAAARADKTWPPTGHTYRGHEDEYPEY
DAYDAINVDSMRMMPDDHDGPMGVPLNFLEHWAPGNGFMLLDNLDDPTIDGRRLYKRLLVRRTRDA

Sequences:

>Translated_226_residues
MACRQRRPRGGYSIADLAARPLDGDGSFDSPECEHLLDRPGAIVVTNPPFSHAIRFMRMLRRHPATDYLIVANLNLTTAN
DVFPMIKEGRCLVGLSIHSGSMFFRLPDDRPKTGSMIRPDGTVGVNSIRWLTSLAAARADKTWPPTGHTYRGHEDEYPEY
DAYDAINVDSMRMMPDDHDGPMGVPLNFLEHWAPGNGFMLLDNLDDPTIDGRRLYKRLLVRRTRDA
>Mature_225_residues
ACRQRRPRGGYSIADLAARPLDGDGSFDSPECEHLLDRPGAIVVTNPPFSHAIRFMRMLRRHPATDYLIVANLNLTTAND
VFPMIKEGRCLVGLSIHSGSMFFRLPDDRPKTGSMIRPDGTVGVNSIRWLTSLAAARADKTWPPTGHTYRGHEDEYPEYD
AYDAINVDSMRMMPDDHDGPMGVPLNFLEHWAPGNGFMLLDNLDDPTIDGRRLYKRLLVRRTRDA

Specific function: This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052 [H]

Pfam domain/function: NA

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 25464; Mature: 25333

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACRQRRPRGGYSIADLAARPLDGDGSFDSPECEHLLDRPGAIVVTNPPFSHAIRFMRML
CCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
RRHPATDYLIVANLNLTTANDVFPMIKEGRCLVGLSIHSGSMFFRLPDDRPKTGSMIRPD
HHCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEEECCEEEEECCCCCCCCCCEECCC
GTVGVNSIRWLTSLAAARADKTWPPTGHTYRGHEDEYPEYDAYDAINVDSMRMMPDDHDG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCC
PMGVPLNFLEHWAPGNGFMLLDNLDDPTIDGRRLYKRLLVRRTRDA
CCCCCHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ACRQRRPRGGYSIADLAARPLDGDGSFDSPECEHLLDRPGAIVVTNPPFSHAIRFMRML
CCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
RRHPATDYLIVANLNLTTANDVFPMIKEGRCLVGLSIHSGSMFFRLPDDRPKTGSMIRPD
HHCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEEECCEEEEECCCCCCCCCCEECCC
GTVGVNSIRWLTSLAAARADKTWPPTGHTYRGHEDEYPEYDAYDAINVDSMRMMPDDHDG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCC
PMGVPLNFLEHWAPGNGFMLLDNLDDPTIDGRRLYKRLLVRRTRDA
CCCCCHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6257703; 6257701; 6257702 [H]