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The map label for this gene is gph [C]

Identifier: 213692056

GI number: 213692056

Start: 1360272

End: 1360991

Strand: Direct

Name: gph [C]

Synonym: Blon_1174

Alternate gene names: 213692056

Gene position: 1360272-1360991 (Clockwise)

Preceding gene: 213692055

Following gene: 213692059

Centisome position: 48.02

GC content: 57.92

Gene sequence:

>720_bases
ATGGCACAGCATCCGATGAAAGTGGTCCTGTTGGATCTCGACGGCACGCTTACCAAGTCCGACGGCGGCATTATCGGCTC
CGTCATCAAGACCTTCGAAGAACTTGGACGTCCGGTTCCGGACGCCGCCGAACTGCGCCGCTTCATTGGACCGGCGATCA
TCGAATCTCTGCGTCGCAATCATGTGCCTGCGGAAGATTTGGATCGGGCCGTGGATATCTACCGCAGCTACTACGGAGAC
CGTGCCGTGTTCGACGATCCGAACGAGCCCGGCAACAAGGTGCCCGGACGACTGGTGAACGTCGTATTCCCAGGCATCCG
TGAACAGCTGCTCAAACTGCATGCCGACGGTTATTACCTGGCACTGGCCAGCTGCAAGCCCGAATACCAGTGCATTCCTA
TCTGCGACCATTTCCACCTGACCGAACTGTTGGACGGCATCTATGGTGCCTCCAAAGACAATTCCCGCCTGGACAAAGAT
CAGGTGATTCGATACTGCTTCGACAAAATCGCGTTTGATGCCGCAGCGGGGGACAAGGCCGTGATGATCGGCGACCGCTA
CACCGACATCGACGGCGCCCACGCCTGCAATCTGGACGCCATTGGCTGCCGCTGGGGCTACGCTCCGGCTGGTGAGATGG
AAGAACACGGTGCCTACGAAATCATTGAGCAACCTGAGCAGCTCGAAGACGCCGTGAACCGATACTTCGAGATGCACTGA

Upstream 100 bases:

>100_bases
CCAGTCATCCCGACTGGGCATAATCATTGTTGGAACAAGTCGCATATTGTTTGACGACCATTCAAAGCAGTCGACATGAA
CAAGGGGGAGTGGAATCTCA

Downstream 100 bases:

>100_bases
CTGATTGCTCCCACCGGCGGGAGCTTCTAACGACGCTGACTGAGGGTGCTTACTACATGAGTAGAAGCACCCTCAGTGGC
GCTAGGTGAAGCAACCCCTG

Product: Haloacid dehalogenase domain protein hydrolase

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MAQHPMKVVLLDLDGTLTKSDGGIIGSVIKTFEELGRPVPDAAELRRFIGPAIIESLRRNHVPAEDLDRAVDIYRSYYGD
RAVFDDPNEPGNKVPGRLVNVVFPGIREQLLKLHADGYYLALASCKPEYQCIPICDHFHLTELLDGIYGASKDNSRLDKD
QVIRYCFDKIAFDAAAGDKAVMIGDRYTDIDGAHACNLDAIGCRWGYAPAGEMEEHGAYEIIEQPEQLEDAVNRYFEMH

Sequences:

>Translated_239_residues
MAQHPMKVVLLDLDGTLTKSDGGIIGSVIKTFEELGRPVPDAAELRRFIGPAIIESLRRNHVPAEDLDRAVDIYRSYYGD
RAVFDDPNEPGNKVPGRLVNVVFPGIREQLLKLHADGYYLALASCKPEYQCIPICDHFHLTELLDGIYGASKDNSRLDKD
QVIRYCFDKIAFDAAAGDKAVMIGDRYTDIDGAHACNLDAIGCRWGYAPAGEMEEHGAYEIIEQPEQLEDAVNRYFEMH
>Mature_238_residues
AQHPMKVVLLDLDGTLTKSDGGIIGSVIKTFEELGRPVPDAAELRRFIGPAIIESLRRNHVPAEDLDRAVDIYRSYYGDR
AVFDDPNEPGNKVPGRLVNVVFPGIREQLLKLHADGYYLALASCKPEYQCIPICDHFHLTELLDGIYGASKDNSRLDKDQ
VIRYCFDKIAFDAAAGDKAVMIGDRYTDIDGAHACNLDAIGCRWGYAPAGEMEEHGAYEIIEQPEQLEDAVNRYFEMH

Specific function: Unknown

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 26702; Mature: 26571

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQHPMKVVLLDLDGTLTKSDGGIIGSVIKTFEELGRPVPDAAELRRFIGPAIIESLRRN
CCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHC
HVPAEDLDRAVDIYRSYYGDRAVFDDPNEPGNKVPGRLVNVVFPGIREQLLKLHADGYYL
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
ALASCKPEYQCIPICDHFHLTELLDGIYGASKDNSRLDKDQVIRYCFDKIAFDAAAGDKA
EEECCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
VMIGDRYTDIDGAHACNLDAIGCRWGYAPAGEMEEHGAYEIIEQPEQLEDAVNRYFEMH
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AQHPMKVVLLDLDGTLTKSDGGIIGSVIKTFEELGRPVPDAAELRRFIGPAIIESLRRN
CCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHC
HVPAEDLDRAVDIYRSYYGDRAVFDDPNEPGNKVPGRLVNVVFPGIREQLLKLHADGYYL
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
ALASCKPEYQCIPICDHFHLTELLDGIYGASKDNSRLDKDQVIRYCFDKIAFDAAAGDKA
EEECCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
VMIGDRYTDIDGAHACNLDAIGCRWGYAPAGEMEEHGAYEIIEQPEQLEDAVNRYFEMH
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12368430; 12218036 [H]