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The map label for this gene is pgk [H]

Identifier: 213691969

GI number: 213691969

Start: 1273517

End: 1274722

Strand: Direct

Name: pgk [H]

Synonym: Blon_1087

Alternate gene names: 213691969

Gene position: 1273517-1274722 (Clockwise)

Preceding gene: 213691968

Following gene: 213691970

Centisome position: 44.96

GC content: 64.84

Gene sequence:

>1206_bases
ATGAAGACACTCAAGGACCTTGGAGATCTCAAGGGCAAGCGCGTTCTGGTTCGCGCTGATTTTAATGTTCCGCTGGACGG
CACCACCATCACCGATGACGGTCGAATCAAGGCTGCTCTGCCGACCATCAAGACCCTGCGCGAAGAAGGCGCCAAGGTCA
TCCTCATGGCCCACCTTGGCCGCCCGAAGGGCAAGGTCGTTCCTGAGCTGAGCCTGGCCCCGGTTGCCGCCCGCCTCGGC
GAGCTGCTCGGCACCAACGTGCCGCTGGCCAAGGACACCTACGGCGAGGACGCTCAGGCCAAGGTCGCCGCCATGAACGA
TGGCGACGTTGTGCTGCTTGAGAACGTTCGTTTCAACCCGGAGGAGACCTCCAAGGACGCCGACGAACGCGCCGCTTACG
CCAAGAAGATCGCCGCCCTCGGCGAGGCCTTCGTTTCCGATGGCTTCGGCGTGGTCCACCGCGCCCAGGGCTCCAACTAC
GACGTTGCCGCCGATCTGCCGGCCGCCGCAGGCCTGCTGGTCGAGAAGGAAGTCAAGGCCCTGTCCAAGGCCACCGAGAA
CCCGGAGCGTCCGTTCACCGTGGTGCTCGGCGGTTCCAAGGTCTCCGACAAGCTCGGCGTCATCGAGAACCTGCTCGACA
AGGCCAACCGTCTGGTCATCGGCGGCGGCATGGTGTTCACCTTCCTCAAGGCCAAGGGCTACGAGGTCGGCACCTCCCTG
CTTGAAGAGGACCAGCTTGAGAAGGTCAAGGGCTACATCGAGACCGCCGAGAAGAACGGCGTTGAGCTCGTGCTGCCGAC
CGACGTCGTCGTGAACGCCGGCTTCCCGGCCGGTGACACCCCGGTTGCTCCTGAAGTCGTTGCCGCCGACGCCATCCCGG
CCGACAAGATGGGTCTGGACATCGGCCCGGAGTCCCAGAAGCTCTTCCACGACAAGATCGTCGACTCCAAGACCGTCGTG
TGGAACGGCCCGATGGGCGTGTTCGAGGTTCCTGAGTTCGCCGCCGGCACCAAGGCCGTGGCCCAGGGTCTGGTCGACGC
CACCGCAGCCGGTGCGTTCACCATCGTCGGTGGCGGCGACTCCGCCTCCGCCGTGCGCAACCTCGGCTTCCCGGAGGACG
GCTTCTCCCACATCTCCACCGGTGGTGGCGCTTCCCTCGAGTTCCTCGAAGGCAAGGAGCTCCCTGGCCTGAAGGTGCTT
GAGTAA

Upstream 100 bases:

>100_bases
GTCAGTAGACGAATGCGGTGTTTTGTTCCGGAATGCCTGTAATATGCAAAGTGGCAAAATTGGCCACTTTGACGTGCGAT
TCGTCAAGGAAAGGATATTC

Downstream 100 bases:

>100_bases
TCCATTTTCTAGCTCCCCTCCTTGAGGGGAGCTACGTATTTTCTAGAAGGGAAACATATGGCGTCCAAACGCATTCCGTT
GGTGGCCGGCAACTGGAAGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKTLKDLGDLKGKRVLVRADFNVPLDGTTITDDGRIKAALPTIKTLREEGAKVILMAHLGRPKGKVVPELSLAPVAARLG
ELLGTNVPLAKDTYGEDAQAKVAAMNDGDVVLLENVRFNPEETSKDADERAAYAKKIAALGEAFVSDGFGVVHRAQGSNY
DVAADLPAAAGLLVEKEVKALSKATENPERPFTVVLGGSKVSDKLGVIENLLDKANRLVIGGGMVFTFLKAKGYEVGTSL
LEEDQLEKVKGYIETAEKNGVELVLPTDVVVNAGFPAGDTPVAPEVVAADAIPADKMGLDIGPESQKLFHDKIVDSKTVV
WNGPMGVFEVPEFAAGTKAVAQGLVDATAAGAFTIVGGGDSASAVRNLGFPEDGFSHISTGGGASLEFLEGKELPGLKVL
E

Sequences:

>Translated_401_residues
MKTLKDLGDLKGKRVLVRADFNVPLDGTTITDDGRIKAALPTIKTLREEGAKVILMAHLGRPKGKVVPELSLAPVAARLG
ELLGTNVPLAKDTYGEDAQAKVAAMNDGDVVLLENVRFNPEETSKDADERAAYAKKIAALGEAFVSDGFGVVHRAQGSNY
DVAADLPAAAGLLVEKEVKALSKATENPERPFTVVLGGSKVSDKLGVIENLLDKANRLVIGGGMVFTFLKAKGYEVGTSL
LEEDQLEKVKGYIETAEKNGVELVLPTDVVVNAGFPAGDTPVAPEVVAADAIPADKMGLDIGPESQKLFHDKIVDSKTVV
WNGPMGVFEVPEFAAGTKAVAQGLVDATAAGAFTIVGGGDSASAVRNLGFPEDGFSHISTGGGASLEFLEGKELPGLKVL
E
>Mature_401_residues
MKTLKDLGDLKGKRVLVRADFNVPLDGTTITDDGRIKAALPTIKTLREEGAKVILMAHLGRPKGKVVPELSLAPVAARLG
ELLGTNVPLAKDTYGEDAQAKVAAMNDGDVVLLENVRFNPEETSKDADERAAYAKKIAALGEAFVSDGFGVVHRAQGSNY
DVAADLPAAAGLLVEKEVKALSKATENPERPFTVVLGGSKVSDKLGVIENLLDKANRLVIGGGMVFTFLKAKGYEVGTSL
LEEDQLEKVKGYIETAEKNGVELVLPTDVVVNAGFPAGDTPVAPEVVAADAIPADKMGLDIGPESQKLFHDKIVDSKTVV
WNGPMGVFEVPEFAAGTKAVAQGLVDATAAGAFTIVGGGDSASAVRNLGFPEDGFSHISTGGGASLEFLEGKELPGLKVL
E

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=407, Percent_Identity=46.9287469287469, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI31543397, Length=407, Percent_Identity=45.2088452088452, Blast_Score=328, Evalue=5e-90,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=42.713567839196, Blast_Score=273, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17508823, Length=405, Percent_Identity=45.9259259259259, Blast_Score=333, Evalue=9e-92,
Organism=Saccharomyces cerevisiae, GI6319857, Length=411, Percent_Identity=47.4452554744526, Blast_Score=334, Evalue=1e-92,
Organism=Drosophila melanogaster, GI17136394, Length=405, Percent_Identity=45.679012345679, Blast_Score=317, Evalue=7e-87,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=42.1686746987952, Blast_Score=288, Evalue=3e-78,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41896; Mature: 41896

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLKDLGDLKGKRVLVRADFNVPLDGTTITDDGRIKAALPTIKTLREEGAKVILMAHLG
CCCHHHHHCCCCCEEEEEECCCCCCCCCEECCCCEEEEHHHHHHHHHHCCCEEEEEEECC
RPKGKVVPELSLAPVAARLGELLGTNVPLAKDTYGEDAQAKVAAMNDGDVVLLENVRFNP
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCC
EETSKDADERAAYAKKIAALGEAFVSDGFGVVHRAQGSNYDVAADLPAAAGLLVEKEVKA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHH
LSKATENPERPFTVVLGGSKVSDKLGVIENLLDKANRLVIGGGMVFTFLKAKGYEVGTSL
HHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEECHHEEEHHHHCCCHHHHHH
LEEDQLEKVKGYIETAEKNGVELVLPTDVVVNAGFPAGDTPVAPEVVAADAIPADKMGLD
HHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCCCCHHHHHCCCCCHHCCCC
IGPESQKLFHDKIVDSKTVVWNGPMGVFEVPEFAAGTKAVAQGLVDATAAGAFTIVGGGD
CCCCHHHHHHHHHCCCCEEEECCCCCCEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCC
SASAVRNLGFPEDGFSHISTGGGASLEFLEGKELPGLKVLE
CHHHHHHCCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKTLKDLGDLKGKRVLVRADFNVPLDGTTITDDGRIKAALPTIKTLREEGAKVILMAHLG
CCCHHHHHCCCCCEEEEEECCCCCCCCCEECCCCEEEEHHHHHHHHHHCCCEEEEEEECC
RPKGKVVPELSLAPVAARLGELLGTNVPLAKDTYGEDAQAKVAAMNDGDVVLLENVRFNP
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCC
EETSKDADERAAYAKKIAALGEAFVSDGFGVVHRAQGSNYDVAADLPAAAGLLVEKEVKA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHH
LSKATENPERPFTVVLGGSKVSDKLGVIENLLDKANRLVIGGGMVFTFLKAKGYEVGTSL
HHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEECHHEEEHHHHCCCHHHHHH
LEEDQLEKVKGYIETAEKNGVELVLPTDVVVNAGFPAGDTPVAPEVVAADAIPADKMGLD
HHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCCCCHHHHHCCCCCHHCCCC
IGPESQKLFHDKIVDSKTVVWNGPMGVFEVPEFAAGTKAVAQGLVDATAAGAFTIVGGGD
CCCCHHHHHHHHHCCCCEEEECCCCCCEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCC
SASAVRNLGFPEDGFSHISTGGGASLEFLEGKELPGLKVLE
CHHHHHHCCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA