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The map label for this gene is hisH [H]

Identifier: 213691722

GI number: 213691722

Start: 973624

End: 974271

Strand: Direct

Name: hisH [H]

Synonym: Blon_0827

Alternate gene names: 213691722

Gene position: 973624-974271 (Clockwise)

Preceding gene: 213691721

Following gene: 213691723

Centisome position: 34.37

GC content: 62.96

Gene sequence:

>648_bases
ATGACCACAGCGGTTGTTTTCGATTACGGTTTCGGCAATGTACGTTCCATGGTGCGTGCGCTCGCCAATCTTGGCGTCGA
CACCACTCTCACCTCCGATTATCGTCAGGCGCTGGAGGCCGACGGTCTGGTCGTGCCGGGCGTGGGCGCGTTCGCCGCAT
GTATGGAGGGTCTGAAGAAGGTCGACGGCGACCGTGTGATCTATGATCGCATCCGCGCCGGCCGTCCGGTGCTTGGCGTG
TGCGTGGGCGAGCAGATCATGTTCGAACACGGTCTGGAGCACGGGGTCCATGCCGCGGGCATCGGGCTGATCGGCGGTTC
GGTGAACCTGCTGGACGCCGACGTGGTGCCTCACATGGGATGGGACACGATCGAGGCCGCCCCCGATTCGGTGCTGCTCA
ACGGCGTGGAGAACGAACGGTTCTACTTCGTGCACTCCTACGCCGCGATGGAAGTGAAGCCGGTGGATACGTCTCGTTTC
GACATTGATCTGAGCGGTGCGCCCGAACGCGTGAGCTGGTGCTCGTACGGCCGGTCGCGTTTCGTGGCCGCCTATGAACA
CGGCCCGCTGTCCGCCACGCAATTCCACCCGGAAAAGTCCGGAGACGCCGGTGCCCAGCTCCTCAAGAACTGGATCGCCA
CACTGTAA

Upstream 100 bases:

>100_bases
GGCGATGCAGATTCTCGCCAACCACACCAGACGCGGACGTGGCGGGCGCGGCTCCCGCATCGAATAGACGCCAACCAACC
AGTAGGGGATAGGAGAGAAT

Downstream 100 bases:

>100_bases
AAAGCATGAAGCTCCCTCTGACGAGAGAGCTCAAATCGTGTATATGTAACTGAAGAAAGGCTCTTCTTATGTCGTTGACT
CTGCTGCCCGCCGTCGATGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTTAVVFDYGFGNVRSMVRALANLGVDTTLTSDYRQALEADGLVVPGVGAFAACMEGLKKVDGDRVIYDRIRAGRPVLGV
CVGEQIMFEHGLEHGVHAAGIGLIGGSVNLLDADVVPHMGWDTIEAAPDSVLLNGVENERFYFVHSYAAMEVKPVDTSRF
DIDLSGAPERVSWCSYGRSRFVAAYEHGPLSATQFHPEKSGDAGAQLLKNWIATL

Sequences:

>Translated_215_residues
MTTAVVFDYGFGNVRSMVRALANLGVDTTLTSDYRQALEADGLVVPGVGAFAACMEGLKKVDGDRVIYDRIRAGRPVLGV
CVGEQIMFEHGLEHGVHAAGIGLIGGSVNLLDADVVPHMGWDTIEAAPDSVLLNGVENERFYFVHSYAAMEVKPVDTSRF
DIDLSGAPERVSWCSYGRSRFVAAYEHGPLSATQFHPEKSGDAGAQLLKNWIATL
>Mature_214_residues
TTAVVFDYGFGNVRSMVRALANLGVDTTLTSDYRQALEADGLVVPGVGAFAACMEGLKKVDGDRVIYDRIRAGRPVLGVC
VGEQIMFEHGLEHGVHAAGIGLIGGSVNLLDADVVPHMGWDTIEAAPDSVLLNGVENERFYFVHSYAAMEVKPVDTSRFD
IDLSGAPERVSWCSYGRSRFVAAYEHGPLSATQFHPEKSGDAGAQLLKNWIATL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=221, Percent_Identity=34.3891402714932, Blast_Score=111, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319725, Length=217, Percent_Identity=29.9539170506912, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23145; Mature: 23014

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAVVFDYGFGNVRSMVRALANLGVDTTLTSDYRQALEADGLVVPGVGAFAACMEGLKK
CCEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEECCHHHHHHHHHHHHH
VDGDRVIYDRIRAGRPVLGVCVGEQIMFEHGLEHGVHAAGIGLIGGSVNLLDADVVPHMG
CCCCCEEHHHHHCCCCEEEEHHHHHHHHHHCHHCCCHHHCEEEECCCCEEEECCCCCCCC
WDTIEAAPDSVLLNGVENERFYFVHSYAAMEVKPVDTSRFDIDLSGAPERVSWCSYGRSR
CCHHHCCCCCEEEECCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHCCCHH
FVAAYEHGPLSATQFHPEKSGDAGAQLLKNWIATL
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TTAVVFDYGFGNVRSMVRALANLGVDTTLTSDYRQALEADGLVVPGVGAFAACMEGLKK
CEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEECCHHHHHHHHHHHHH
VDGDRVIYDRIRAGRPVLGVCVGEQIMFEHGLEHGVHAAGIGLIGGSVNLLDADVVPHMG
CCCCCEEHHHHHCCCCEEEEHHHHHHHHHHCHHCCCHHHCEEEECCCCEEEECCCCCCCC
WDTIEAAPDSVLLNGVENERFYFVHSYAAMEVKPVDTSRFDIDLSGAPERVSWCSYGRSR
CCHHHCCCCCEEEECCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHCCCHH
FVAAYEHGPLSATQFHPEKSGDAGAQLLKNWIATL
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]