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The map label for this gene is thiE/thiC [H]

Identifier: 213691691

GI number: 213691691

Start: 922096

End: 924849

Strand: Reverse

Name: thiE/thiC [H]

Synonym: Blon_0796

Alternate gene names: 213691691

Gene position: 924849-922096 (Counterclockwise)

Preceding gene: 213691692

Following gene: 213691689

Centisome position: 32.65

GC content: 64.05

Gene sequence:

>2754_bases
ATGAGTAATGAATACCCATACGCCTCCATGCGAGACAACTTCGACTTGTCCGCGTACTTCGTGGTGGGGCCGGAGGACTG
CAAAGGCCGTCCGCTGACCGACGTGGTGGATCAGGCTCTGCGCGGCGGCGCAACCTTCATCCAACTGCGCGCCAAGGAGG
CCGATGCCTCTGAGCTGACCGACATGGCCCGCGACATCGCGCAGATCATCGAGGACAATGAGAAGTCCGATTCCGTGGCC
TTTGTGATCGACGACCGTGCCGACGTGGTGTGGCAGGCCCGCCGCAAGGGCATCAAGGTGGACGGTGTGCATATCGGCCA
GACCGACATGGAGCCGCGAGAAGCCCGCGCCCTGCTCGGCGACGAGGCCATCGTGGGTCTGTCGGCTGAGACCGAAAGCC
TCGTTCAGCTCATCAACGAGCTGCCCGACGGCTGCATCGACTACATTGGCGCCGGCCCTCTGCACGTCTCCACCACCAAG
CCCGAGGCTTCCGTGGGCGGCAACGATGGCTCCGGCAAGACACTGGACGCCGCCCAGATCAACACCATCTGCGCTGCCAG
CGAATTCCCGGTGGTCGTGGGCGGCGGCGTGACCGCAGCCGATATGGCCATGCTCGCCGGCACCAAGGCCGCCGGCTGGT
TCGCGGTCTCCGCCATCGCGGGTGCCGAGAACCCGGAAGAGGCCGCGCGCACCATGGTCGAAGGCTGGAAGGCCGTCCGC
GGCGACAAGAAGCACGGCTACGCGCCGCGCGTCGTAGCCCACACCCCCGCTGCTGATACTCAGGCTGCTCAGGAAGGGGC
CGCAAAGCCTGGCTCCGAGGCCACTGAGAAGAAGTTCACCAACGCCAAGGACGCCAAGGATGCCCAGAAGCTCGCCAAGC
AGCAGCGCGTGGACATCGCCGCCCGTGGTTCCAAGCAACGCGACAAGGCGCACATCCGCAAGACCAAGTCCGTACCGTTC
ACCTATCAGTATGGTTCCTACGACCTGGAAGTGCCGTACACCGAAATCAAGCTGAGCGACACGCCCGGCGTGGGCCCGAA
CCCGCCGTTCCACGACTACAACACCGAAGGCCCCAAGTGCGACCCGAAGGAAGGTTTGAAGCCCCTGCGTCTCGACTGGA
TCCGCGACCGCGGCGACATCGAAGACTACGAGGGCCGCCGCCGCAACCTTGAGGATGATGGTAAGCGCGCCATCAAGCGC
GGCCGCGCCACCAAGGAATGGCGTGGACGCAAGCATGAGCCGATGCGCGCCAAGGACCACCCGATCACCCAGATGTGGTA
CGCCCGCCACGGCATCATCACCCCGGAGATGCAGTACGTGGCCACCCGCGAGAACTGCGATGTGGAGCTCGTGCGCTCCG
AGCTGGCCGCCGGCCGCGCGGTGATGCCCTGCAACATCAACCACCCCGAGGCCGAGCCGATGATTATCGGATCCGCCTTC
CTGACCAAGCTCAACGCCAACATGGGCAACTCGGCCGTCACCTCCTCCATCGACGAGGAAGTGGAGAAGCTGACGTGGGC
CACCAAGTGGGGCGCCGATACCGTGATGGACCTGTCCACCGGTAACGACATCCACACCACCCGTGAGTGGATTCTTCGCA
ACTCCCCCGTGCCGATCGGCACCGTGCCGATGTACCAGGCCCTCGAAAAGGTCGAGGATGATGCCTCCAAGCTGAGCTGG
GAACTGTTCCGCGACACCGTGATCGAACAGTGCGAGCAGGGTGTGGATTACATGACCATCCACGCCGGCGTGCTGCTGCG
TTACGTGCCGTTGACCGCCAACCGCGTGACCGGCATCGTCTCCCGCGGTGGCTCCATCATGGCCGACTGGTGCCTGCGCC
ACCACCAGGAGAGCTTCCTGTACACGCACTTCGACGAGCTGTGCGACATCTTCGCCAAGTACGACGTGGCGTTCTCTCTG
GGCGATGGCCTGCGCCCCGGCTCCCTGGCCGACGCGAACGACGCCGCGCAGCTTTCCGAGCTCATGACTCTGGGCGAGCT
GACCGAGCGCGCCTGGGCCAAGGACGTGCAGGTGATGATCGAGGGCCCGGGCCACGTGCCGTTCGATACCGTACGCATGA
ACATCGAACTGGAGAAGTCGGTGTGCAAGGGCGCCCCGTTCTACACCCTCGGACCGCTGACCACCGATACCGCTCCGGGT
TACGACCACATCACCTCCGCCATCGGCGCCACCGAGATCGGTCGTTACGGCACCGCTATGCTGTGCTACGTGACCCCGAA
GGAACACCTCGGCCTGCCGAACAAGGATGATGTGAAGCAGGGCGTGATCGCCTACAAGATCGCCTGCCACGCCGCAGACA
TCGCCAAGCACCACCCGCACGCGATGGATCGTGACAATGCGATTTCCAAGGCTCGCTTCGAGTTCCGTTGGCTCGACCAG
TTCAATCTGTCCTACGATCCGGACACCGCCATCGCCTTCCACGATGACACCCTGCCCGCCGAGCCGGCCAAGATGGCGCA
CTTCTGCTCGATGTGCGGCCCGAAGTTCTGCTCGATGGCCATCTCCCAGAACATCCGCAAGGCATTCGGTGGCGAGGCCG
CTCAGCAGCAAATCGTGAAGGAGGCTGCTGCTGGCATCGACTCCGAGGCACTGGCTAAGGCCAAGGCCAATGTCGATAAC
GACGTGGTGTCCGCCAACGTGCTCAGCCCCGAAGAAATCCTCGCCGGCATGGATGCGATGAGCGAAAAGTACACCGCCCA
AGGCGGCAAGCTCTACTCCACTGCCCAGGAGTAA

Upstream 100 bases:

>100_bases
GAGATTTTGGTGCAGTAGTTTCAGTTCCCTCTGATGAGGGAGCTCATAACAGTTTTTCCCAATAAGACTTCCACAAGAAA
CAACAAAGGTAGGAATAATA

Downstream 100 bases:

>100_bases
ACCTCTCTGGCTCCCCTCTCTGAGGGGAGCCAATTATTATGCCCGGTGGTGTCTTAATTCGGGACGACGCTCGACTCGCT
CGAAGGCGGTGACGAGGTCG

Product: thiamine biosynthesis protein ThiC

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Phosphomethylpyrimidine synthase; Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 917; Mature: 916

Protein sequence:

>917_residues
MSNEYPYASMRDNFDLSAYFVVGPEDCKGRPLTDVVDQALRGGATFIQLRAKEADASELTDMARDIAQIIEDNEKSDSVA
FVIDDRADVVWQARRKGIKVDGVHIGQTDMEPREARALLGDEAIVGLSAETESLVQLINELPDGCIDYIGAGPLHVSTTK
PEASVGGNDGSGKTLDAAQINTICAASEFPVVVGGGVTAADMAMLAGTKAAGWFAVSAIAGAENPEEAARTMVEGWKAVR
GDKKHGYAPRVVAHTPAADTQAAQEGAAKPGSEATEKKFTNAKDAKDAQKLAKQQRVDIAARGSKQRDKAHIRKTKSVPF
TYQYGSYDLEVPYTEIKLSDTPGVGPNPPFHDYNTEGPKCDPKEGLKPLRLDWIRDRGDIEDYEGRRRNLEDDGKRAIKR
GRATKEWRGRKHEPMRAKDHPITQMWYARHGIITPEMQYVATRENCDVELVRSELAAGRAVMPCNINHPEAEPMIIGSAF
LTKLNANMGNSAVTSSIDEEVEKLTWATKWGADTVMDLSTGNDIHTTREWILRNSPVPIGTVPMYQALEKVEDDASKLSW
ELFRDTVIEQCEQGVDYMTIHAGVLLRYVPLTANRVTGIVSRGGSIMADWCLRHHQESFLYTHFDELCDIFAKYDVAFSL
GDGLRPGSLADANDAAQLSELMTLGELTERAWAKDVQVMIEGPGHVPFDTVRMNIELEKSVCKGAPFYTLGPLTTDTAPG
YDHITSAIGATEIGRYGTAMLCYVTPKEHLGLPNKDDVKQGVIAYKIACHAADIAKHHPHAMDRDNAISKARFEFRWLDQ
FNLSYDPDTAIAFHDDTLPAEPAKMAHFCSMCGPKFCSMAISQNIRKAFGGEAAQQQIVKEAAAGIDSEALAKAKANVDN
DVVSANVLSPEEILAGMDAMSEKYTAQGGKLYSTAQE

Sequences:

>Translated_917_residues
MSNEYPYASMRDNFDLSAYFVVGPEDCKGRPLTDVVDQALRGGATFIQLRAKEADASELTDMARDIAQIIEDNEKSDSVA
FVIDDRADVVWQARRKGIKVDGVHIGQTDMEPREARALLGDEAIVGLSAETESLVQLINELPDGCIDYIGAGPLHVSTTK
PEASVGGNDGSGKTLDAAQINTICAASEFPVVVGGGVTAADMAMLAGTKAAGWFAVSAIAGAENPEEAARTMVEGWKAVR
GDKKHGYAPRVVAHTPAADTQAAQEGAAKPGSEATEKKFTNAKDAKDAQKLAKQQRVDIAARGSKQRDKAHIRKTKSVPF
TYQYGSYDLEVPYTEIKLSDTPGVGPNPPFHDYNTEGPKCDPKEGLKPLRLDWIRDRGDIEDYEGRRRNLEDDGKRAIKR
GRATKEWRGRKHEPMRAKDHPITQMWYARHGIITPEMQYVATRENCDVELVRSELAAGRAVMPCNINHPEAEPMIIGSAF
LTKLNANMGNSAVTSSIDEEVEKLTWATKWGADTVMDLSTGNDIHTTREWILRNSPVPIGTVPMYQALEKVEDDASKLSW
ELFRDTVIEQCEQGVDYMTIHAGVLLRYVPLTANRVTGIVSRGGSIMADWCLRHHQESFLYTHFDELCDIFAKYDVAFSL
GDGLRPGSLADANDAAQLSELMTLGELTERAWAKDVQVMIEGPGHVPFDTVRMNIELEKSVCKGAPFYTLGPLTTDTAPG
YDHITSAIGATEIGRYGTAMLCYVTPKEHLGLPNKDDVKQGVIAYKIACHAADIAKHHPHAMDRDNAISKARFEFRWLDQ
FNLSYDPDTAIAFHDDTLPAEPAKMAHFCSMCGPKFCSMAISQNIRKAFGGEAAQQQIVKEAAAGIDSEALAKAKANVDN
DVVSANVLSPEEILAGMDAMSEKYTAQGGKLYSTAQE
>Mature_916_residues
SNEYPYASMRDNFDLSAYFVVGPEDCKGRPLTDVVDQALRGGATFIQLRAKEADASELTDMARDIAQIIEDNEKSDSVAF
VIDDRADVVWQARRKGIKVDGVHIGQTDMEPREARALLGDEAIVGLSAETESLVQLINELPDGCIDYIGAGPLHVSTTKP
EASVGGNDGSGKTLDAAQINTICAASEFPVVVGGGVTAADMAMLAGTKAAGWFAVSAIAGAENPEEAARTMVEGWKAVRG
DKKHGYAPRVVAHTPAADTQAAQEGAAKPGSEATEKKFTNAKDAKDAQKLAKQQRVDIAARGSKQRDKAHIRKTKSVPFT
YQYGSYDLEVPYTEIKLSDTPGVGPNPPFHDYNTEGPKCDPKEGLKPLRLDWIRDRGDIEDYEGRRRNLEDDGKRAIKRG
RATKEWRGRKHEPMRAKDHPITQMWYARHGIITPEMQYVATRENCDVELVRSELAAGRAVMPCNINHPEAEPMIIGSAFL
TKLNANMGNSAVTSSIDEEVEKLTWATKWGADTVMDLSTGNDIHTTREWILRNSPVPIGTVPMYQALEKVEDDASKLSWE
LFRDTVIEQCEQGVDYMTIHAGVLLRYVPLTANRVTGIVSRGGSIMADWCLRHHQESFLYTHFDELCDIFAKYDVAFSLG
DGLRPGSLADANDAAQLSELMTLGELTERAWAKDVQVMIEGPGHVPFDTVRMNIELEKSVCKGAPFYTLGPLTTDTAPGY
DHITSAIGATEIGRYGTAMLCYVTPKEHLGLPNKDDVKQGVIAYKIACHAADIAKHHPHAMDRDNAISKARFEFRWLDQF
NLSYDPDTAIAFHDDTLPAEPAKMAHFCSMCGPKFCSMAISQNIRKAFGGEAAQQQIVKEAAAGIDSEALAKAKANVDND
VVSANVLSPEEILAGMDAMSEKYTAQGGKLYSTAQE

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=636, Percent_Identity=56.4465408805032, Blast_Score=689, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325042, Length=172, Percent_Identity=31.9767441860465, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR002817
- InterPro:   IPR003733 [H]

Pfam domain/function: PF01964 ThiC; PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 100235; Mature: 100104

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEYPYASMRDNFDLSAYFVVGPEDCKGRPLTDVVDQALRGGATFIQLRAKEADASELT
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHH
DMARDIAQIIEDNEKSDSVAFVIDDRADVVWQARRKGIKVDGVHIGQTDMEPREARALLG
HHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCEECCEEECCCCCCHHHHHHHHC
DEAIVGLSAETESLVQLINELPDGCIDYIGAGPLHVSTTKPEASVGGNDGSGKTLDAAQI
CCEEEECCCCHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEECHHHH
NTICAASEFPVVVGGGVTAADMAMLAGTKAAGWFAVSAIAGAENPEEAARTMVEGWKAVR
HHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHC
GDKKHGYAPRVVAHTPAADTQAAQEGAAKPGSEATEKKFTNAKDAKDAQKLAKQQRVDIA
CCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCHH
ARGSKQRDKAHIRKTKSVPFTYQYGSYDLEVPYTEIKLSDTPGVGPNPPFHDYNTEGPKC
HCCCCHHHHHHHHHCCCCCEEEECCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCC
DPKEGLKPLRLDWIRDRGDIEDYEGRRRNLEDDGKRAIKRGRATKEWRGRKHEPMRAKDH
CHHHCCCCEEHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCC
PITQMWYARHGIITPEMQYVATRENCDVELVRSELAAGRAVMPCNINHPEAEPMIIGSAF
CHHHHHHHHCCCCCCCHHEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEHHHH
LTKLNANMGNSAVTSSIDEEVEKLTWATKWGADTVMDLSTGNDIHTTREWILRNSPVPIG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCC
TVPMYQALEKVEDDASKLSWELFRDTVIEQCEQGVDYMTIHAGVLLRYVPLTANRVTGIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHH
SRGGSIMADWCLRHHQESFLYTHFDELCDIFAKYDVAFSLGDGLRPGSLADANDAAQLSE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHH
LMTLGELTERAWAKDVQVMIEGPGHVPFDTVRMNIELEKSVCKGAPFYTLGPLTTDTAPG
HHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEEEHHHHHCCCCCEEECCCCCCCCCC
YDHITSAIGATEIGRYGTAMLCYVTPKEHLGLPNKDDVKQGVIAYKIACHAADIAKHHPH
HHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCC
AMDRDNAISKARFEFRWLDQFNLSYDPDTAIAFHDDTLPAEPAKMAHFCSMCGPKFCSMA
CCCCCCCHHHHHEEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHH
ISQNIRKAFGGEAAQQQIVKEAAAGIDSEALAKAKANVDNDVVSANVLSPEEILAGMDAM
HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHH
SEKYTAQGGKLYSTAQE
HHHHHCCCCEEECCCCC
>Mature Secondary Structure 
SNEYPYASMRDNFDLSAYFVVGPEDCKGRPLTDVVDQALRGGATFIQLRAKEADASELT
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHH
DMARDIAQIIEDNEKSDSVAFVIDDRADVVWQARRKGIKVDGVHIGQTDMEPREARALLG
HHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCEECCEEECCCCCCHHHHHHHHC
DEAIVGLSAETESLVQLINELPDGCIDYIGAGPLHVSTTKPEASVGGNDGSGKTLDAAQI
CCEEEECCCCHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEECHHHH
NTICAASEFPVVVGGGVTAADMAMLAGTKAAGWFAVSAIAGAENPEEAARTMVEGWKAVR
HHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHC
GDKKHGYAPRVVAHTPAADTQAAQEGAAKPGSEATEKKFTNAKDAKDAQKLAKQQRVDIA
CCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCHH
ARGSKQRDKAHIRKTKSVPFTYQYGSYDLEVPYTEIKLSDTPGVGPNPPFHDYNTEGPKC
HCCCCHHHHHHHHHCCCCCEEEECCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCC
DPKEGLKPLRLDWIRDRGDIEDYEGRRRNLEDDGKRAIKRGRATKEWRGRKHEPMRAKDH
CHHHCCCCEEHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCC
PITQMWYARHGIITPEMQYVATRENCDVELVRSELAAGRAVMPCNINHPEAEPMIIGSAF
CHHHHHHHHCCCCCCCHHEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEHHHH
LTKLNANMGNSAVTSSIDEEVEKLTWATKWGADTVMDLSTGNDIHTTREWILRNSPVPIG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCC
TVPMYQALEKVEDDASKLSWELFRDTVIEQCEQGVDYMTIHAGVLLRYVPLTANRVTGIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHH
SRGGSIMADWCLRHHQESFLYTHFDELCDIFAKYDVAFSLGDGLRPGSLADANDAAQLSE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHH
LMTLGELTERAWAKDVQVMIEGPGHVPFDTVRMNIELEKSVCKGAPFYTLGPLTTDTAPG
HHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEEEHHHHHCCCCCEEECCCCCCCCCC
YDHITSAIGATEIGRYGTAMLCYVTPKEHLGLPNKDDVKQGVIAYKIACHAADIAKHHPH
HHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCC
AMDRDNAISKARFEFRWLDQFNLSYDPDTAIAFHDDTLPAEPAKMAHFCSMCGPKFCSMA
CCCCCCCHHHHHEEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHH
ISQNIRKAFGGEAAQQQIVKEAAAGIDSEALAKAKANVDNDVVSANVLSPEEILAGMDAM
HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHH
SEKYTAQGGKLYSTAQE
HHHHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]