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The map label for this gene is thiDE [H]

Identifier: 213691689

GI number: 213691689

Start: 919915

End: 920733

Strand: Reverse

Name: thiDE [H]

Synonym: Blon_0794

Alternate gene names: 213691689

Gene position: 920733-919915 (Counterclockwise)

Preceding gene: 213691691

Following gene: 213691688

Centisome position: 32.5

GC content: 59.83

Gene sequence:

>819_bases
ATGAACACTATGGCACAGATTGCAACATCTACACTTCCCGCTGTTCTCGCGATCTCCGGCTCCGACTCGTCCGGCGGTGC
CGGCATGCAGGCCGATCTGAAGACCATGCTGGCTTGCGGCGTGTTCGGCATGAGCGCCATCACCGCGCTCACCGCACAGA
ACACCACCGGCGTGCGTTCCATTCAGGATACCAAACCGGACATTCTCGCCGACCAGATCAACATGGTGTTTGAGGATATT
CCGCCGGTCGCGGTGAAAATCGGCATGGTGTCTGCCACTGACATCATCGACGTCATCGCTGAACGACTCACCTTCCATCA
CGCCACGAACATCGTGCTCGACCCGGTTATGGTGGCCACTTCCGGTGCCAAGCTCATCAGTGACGATGCCATTGCGGCCT
TGACCGGCAAGCTGTTCCCGATGGCTACGGTCGTGACTCCGAATATTCCCGAGGCCGAAGTGCTGACCGATACGCTGATT
CGCGATCAGGAGGATATGGAGGCCGCCGCCCACCGCATCGTGGAGCGTTACGGCTGTGCCGCGCTGGTCAAGGGTGGTCA
TGGCACCGAGGATGCCAACGATGTGCTTGCCGAGACGGATGGAACCGTGACCTGGTTTGATGGCGCGCGTATCAATAATC
CGAACACGCACGGCACCGGATGCACGCTGAGCTCCGCCATCGCCTCGTATCTGGCGCTTGGCGACACGTTGCCCAAGGCC
ATCAAGCATGCGAAGAAGTACCTGACCGGTGCGCTGAAGGCCCAGCTGAATCTTGGCCACGGCTCCGGCCCGATGGATCA
TCTCTGGAAGTATCGCTGA

Upstream 100 bases:

>100_bases
TCATGAATCAATCGCAATCAGCAAACCCTCCCATCTCACCACAGGAGCGGCAAAACATCAAGGAAAACCGCTCCGAAAAA
CATCATGGGCCCCACTTAGA

Downstream 100 bases:

>100_bases
CATAAGTGTGGTTGTTAAGCTCCCCCTGTCAGGGGAGCTTAACACTACTGAAAAAGGAGAATCATGGTTCTTGACCGTAA
TGCCGTTACCCAGGAAGTGC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNTMAQIATSTLPAVLAISGSDSSGGAGMQADLKTMLACGVFGMSAITALTAQNTTGVRSIQDTKPDILADQINMVFEDI
PPVAVKIGMVSATDIIDVIAERLTFHHATNIVLDPVMVATSGAKLISDDAIAALTGKLFPMATVVTPNIPEAEVLTDTLI
RDQEDMEAAAHRIVERYGCAALVKGGHGTEDANDVLAETDGTVTWFDGARINNPNTHGTGCTLSSAIASYLALGDTLPKA
IKHAKKYLTGALKAQLNLGHGSGPMDHLWKYR

Sequences:

>Translated_272_residues
MNTMAQIATSTLPAVLAISGSDSSGGAGMQADLKTMLACGVFGMSAITALTAQNTTGVRSIQDTKPDILADQINMVFEDI
PPVAVKIGMVSATDIIDVIAERLTFHHATNIVLDPVMVATSGAKLISDDAIAALTGKLFPMATVVTPNIPEAEVLTDTLI
RDQEDMEAAAHRIVERYGCAALVKGGHGTEDANDVLAETDGTVTWFDGARINNPNTHGTGCTLSSAIASYLALGDTLPKA
IKHAKKYLTGALKAQLNLGHGSGPMDHLWKYR
>Mature_272_residues
MNTMAQIATSTLPAVLAISGSDSSGGAGMQADLKTMLACGVFGMSAITALTAQNTTGVRSIQDTKPDILADQINMVFEDI
PPVAVKIGMVSATDIIDVIAERLTFHHATNIVLDPVMVATSGAKLISDDAIAALTGKLFPMATVVTPNIPEAEVLTDTLI
RDQEDMEAAAHRIVERYGCAALVKGGHGTEDANDVLAETDGTVTWFDGARINNPNTHGTGCTLSSAIASYLALGDTLPKA
IKHAKKYLTGALKAQLNLGHGSGPMDHLWKYR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=259, Percent_Identity=44.4015444015444, Blast_Score=197, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6324997, Length=282, Percent_Identity=35.8156028368794, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324517, Length=280, Percent_Identity=35.3571428571429, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325378, Length=277, Percent_Identity=31.4079422382672, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28485; Mature: 28485

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTMAQIATSTLPAVLAISGSDSSGGAGMQADLKTMLACGVFGMSAITALTAQNTTGVRS
CCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IQDTKPDILADQINMVFEDIPPVAVKIGMVSATDIIDVIAERLTFHHATNIVLDPVMVAT
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
SGAKLISDDAIAALTGKLFPMATVVTPNIPEAEVLTDTLIRDQEDMEAAAHRIVERYGCA
CCCHHHCCCHHHHHHHCHHHEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
ALVKGGHGTEDANDVLAETDGTVTWFDGARINNPNTHGTGCTLSSAIASYLALGDTLPKA
EEEECCCCCCCHHHHHHCCCCCEEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IKHAKKYLTGALKAQLNLGHGSGPMDHLWKYR
HHHHHHHHHHHHHHEEECCCCCCCHHHHCCCC
>Mature Secondary Structure
MNTMAQIATSTLPAVLAISGSDSSGGAGMQADLKTMLACGVFGMSAITALTAQNTTGVRS
CCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IQDTKPDILADQINMVFEDIPPVAVKIGMVSATDIIDVIAERLTFHHATNIVLDPVMVAT
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
SGAKLISDDAIAALTGKLFPMATVVTPNIPEAEVLTDTLIRDQEDMEAAAHRIVERYGCA
CCCHHHCCCHHHHHHHCHHHEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
ALVKGGHGTEDANDVLAETDGTVTWFDGARINNPNTHGTGCTLSSAIASYLALGDTLPKA
EEEECCCCCCCHHHHHHCCCCCEEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IKHAKKYLTGALKAQLNLGHGSGPMDHLWKYR
HHHHHHHHHHHHHHEEECCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA