Click here to switch to the map view.

The map label for this gene is rihA [H]

Identifier: 213691679

GI number: 213691679

Start: 908853

End: 910052

Strand: Reverse

Name: rihA [H]

Synonym: Blon_0784

Alternate gene names: 213691679

Gene position: 910052-908853 (Counterclockwise)

Preceding gene: 213691680

Following gene: 213691670

Centisome position: 32.13

GC content: 56.67

Gene sequence:

>1200_bases
ATGGTCCAATGTCTTGACCGCATTATTGCCAGCATGGATACCGGTGTTGATGATGCGTTGGCGTTGGCGTATTTGTTGGG
GTCGGCGGATGAGTGCGAGCTGAGCGGTGTGATCGCCAGTTATGGCAATGTGGATGCGAACACGGCTTATGCCAATACTC
GCGCGGTGCTTGATTTGTTTGGTCGGTCGGATATTCCGGTGTTTTTGGGCTCTGAGCATCCTTCTTGGGCTGATGCGTTC
ATTCCTGATGCAGGGTGTGCCCAGTTCCATGGGGATGATGGACTGGGGAATACTTGGCTGGCTGGGTCCGGAGCTCTGGC
TGATGGAGCTGGTGTGCCTGGTGATATGTCTGGGGATGACATGTCTGTGGATGTGCCCGATGGGGTTGTTTCTGTTGGCG
GGTATTTGGTAGGGGACGCTCATGCTCATCCGGCGTTGGGGTCTTATCCTCGTGTGGGTCGCTTTCGGTCTGTGGACCGC
CCCCAGTCGGCTACATCGACAGCTCTCGTCAGCGGGGGCACAAGGAGGTGCTCCGTTGATGCTGCGGATGGGATTGAGTA
TCTGATTGAGCAGGTTCGCGAGTTTGGTCGAAACGTTACCGTGCTGTCTACCGGGCCGTTGACGGATGTGGATGCGGCTA
TTACTCGGGCACCGGATATTGCGTCGAAACTCAGGCTGGTGATGATGGGAGGTACGCTTACCCAACCCGGCAATTGTTGG
GATGCGGTGGCCGAGACGAATATCATCCAAGACCCTGAGGCCGCGAATCGTGTATTTCATTCCGGCGCAGACATCACAAT
GGTGGGACTTGATGTGACTCACCAGTGTTTGCTAACGCGATCGGCGGCCGACCGGTGGCGTGCAACGGGGACCAAGCGCG
GGCGGTTCTTGGCTGATCTCGCGGACTTTTCCATCAAGGCAAATTTGGAAGCCGACACCGCACTGTTCTCTGGCGGCATG
CCGTTGCATGATCCGTTGGCGGCGGCGGTTGCACTTGACTCTTCACTGGTCGATTGTTTTGATCTTGCGTTGCGCGCGGA
GACCAATACCGGTGATTTCAATGGAGTACGAGGCCGGACCACCGGAGACCCGGTTGGGCTGGTGAATCATTCCATGCCGC
ATGTGCATGTGGCACTTGGTGTGGATTCGGGACGTTTTTTGGATGAGTTTGCAGAGAGGATGGCTGAGGTGTGCCGGTAA

Upstream 100 bases:

>100_bases
CTACGAAAGAATGCGTCCTTTCCATGAGGAAAGGACGCATTTTTGTGTATCGGCCGATAAGGTTATTCATAGCGGATCAC
GCTAACGAGGAGGCGATTCC

Downstream 100 bases:

>100_bases
CTCCCCTCAGTCCGCTACACGGACAGCTCGACCGCAATTACGGACAAGCTGCCGCTCGCATTCTTCTGGCTCGACTCTCG
TCTCGCCTTCGCCTACAAAC

Product: Inosine/uridine-preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MVQCLDRIIASMDTGVDDALALAYLLGSADECELSGVIASYGNVDANTAYANTRAVLDLFGRSDIPVFLGSEHPSWADAF
IPDAGCAQFHGDDGLGNTWLAGSGALADGAGVPGDMSGDDMSVDVPDGVVSVGGYLVGDAHAHPALGSYPRVGRFRSVDR
PQSATSTALVSGGTRRCSVDAADGIEYLIEQVREFGRNVTVLSTGPLTDVDAAITRAPDIASKLRLVMMGGTLTQPGNCW
DAVAETNIIQDPEAANRVFHSGADITMVGLDVTHQCLLTRSAADRWRATGTKRGRFLADLADFSIKANLEADTALFSGGM
PLHDPLAAAVALDSSLVDCFDLALRAETNTGDFNGVRGRTTGDPVGLVNHSMPHVHVALGVDSGRFLDEFAERMAEVCR

Sequences:

>Translated_399_residues
MVQCLDRIIASMDTGVDDALALAYLLGSADECELSGVIASYGNVDANTAYANTRAVLDLFGRSDIPVFLGSEHPSWADAF
IPDAGCAQFHGDDGLGNTWLAGSGALADGAGVPGDMSGDDMSVDVPDGVVSVGGYLVGDAHAHPALGSYPRVGRFRSVDR
PQSATSTALVSGGTRRCSVDAADGIEYLIEQVREFGRNVTVLSTGPLTDVDAAITRAPDIASKLRLVMMGGTLTQPGNCW
DAVAETNIIQDPEAANRVFHSGADITMVGLDVTHQCLLTRSAADRWRATGTKRGRFLADLADFSIKANLEADTALFSGGM
PLHDPLAAAVALDSSLVDCFDLALRAETNTGDFNGVRGRTTGDPVGLVNHSMPHVHVALGVDSGRFLDEFAERMAEVCR
>Mature_399_residues
MVQCLDRIIASMDTGVDDALALAYLLGSADECELSGVIASYGNVDANTAYANTRAVLDLFGRSDIPVFLGSEHPSWADAF
IPDAGCAQFHGDDGLGNTWLAGSGALADGAGVPGDMSGDDMSVDVPDGVVSVGGYLVGDAHAHPALGSYPRVGRFRSVDR
PQSATSTALVSGGTRRCSVDAADGIEYLIEQVREFGRNVTVLSTGPLTDVDAAITRAPDIASKLRLVMMGGTLTQPGNCW
DAVAETNIIQDPEAANRVFHSGADITMVGLDVTHQCLLTRSAADRWRATGTKRGRFLADLADFSIKANLEADTALFSGGM
PLHDPLAAAVALDSSLVDCFDLALRAETNTGDFNGVRGRTTGDPVGLVNHSMPHVHVALGVDSGRFLDEFAERMAEVCR

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=212, Percent_Identity=34.9056603773585, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788486, Length=208, Percent_Identity=28.3653846153846, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786213, Length=198, Percent_Identity=32.8282828282828, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 41801; Mature: 41801

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQCLDRIIASMDTGVDDALALAYLLGSADECELSGVIASYGNVDANTAYANTRAVLDLF
CHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHH
GRSDIPVFLGSEHPSWADAFIPDAGCAQFHGDDGLGNTWLAGSGALADGAGVPGDMSGDD
CCCCCCEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCC
MSVDVPDGVVSVGGYLVGDAHAHPALGSYPRVGRFRSVDRPQSATSTALVSGGTRRCSVD
EEEECCCCHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCEEECC
AADGIEYLIEQVREFGRNVTVLSTGPLTDVDAAITRAPDIASKLRLVMMGGTLTQPGNCW
HHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCHHHHEEEEEECCCCCCCCCHH
DAVAETNIIQDPEAANRVFHSGADITMVGLDVTHQCLLTRSAADRWRATGTKRGRFLADL
HHHHHCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ADFSIKANLEADTALFSGGMPLHDPLAAAVALDSSLVDCFDLALRAETNTGDFNGVRGRT
HCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
TGDPVGLVNHSMPHVHVALGVDSGRFLDEFAERMAEVCR
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVQCLDRIIASMDTGVDDALALAYLLGSADECELSGVIASYGNVDANTAYANTRAVLDLF
CHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHH
GRSDIPVFLGSEHPSWADAFIPDAGCAQFHGDDGLGNTWLAGSGALADGAGVPGDMSGDD
CCCCCCEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCC
MSVDVPDGVVSVGGYLVGDAHAHPALGSYPRVGRFRSVDRPQSATSTALVSGGTRRCSVD
EEEECCCCHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCEEECC
AADGIEYLIEQVREFGRNVTVLSTGPLTDVDAAITRAPDIASKLRLVMMGGTLTQPGNCW
HHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCHHHHEEEEEECCCCCCCCCHH
DAVAETNIIQDPEAANRVFHSGADITMVGLDVTHQCLLTRSAADRWRATGTKRGRFLADL
HHHHHCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ADFSIKANLEADTALFSGGMPLHDPLAAAVALDSSLVDCFDLALRAETNTGDFNGVRGRT
HCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
TGDPVGLVNHSMPHVHVALGVDSGRFLDEFAERMAEVCR
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA