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The map label for this gene is braE [H]

Identifier: 213691482

GI number: 213691482

Start: 654619

End: 655701

Strand: Direct

Name: braE [H]

Synonym: Blon_0579

Alternate gene names: 213691482

Gene position: 654619-655701 (Clockwise)

Preceding gene: 213691481

Following gene: 213691483

Centisome position: 23.11

GC content: 60.94

Gene sequence:

>1083_bases
ATGAAGAAGACAATGCTTTCCACCAAACAGTCCTATCTGGTGTGCTTCATCGGCGTCCTTGCCGCATTCGGCGTTGTGAT
GGCGCTGTGCGAATCCGGCTTGGCTGATTCCTACATCAAGGGCATCATGATGACCGCGTGCATCGCCATTATCATGACCA
CCTCGCTGAACCTCACCATCGGCGTGCTCGGACAGCTGACGTTGGGCTGCTGCGGGTTCGAGGCCATCGGTGCCTACGTG
GCTGCGCTGGCCTCCAAGTACATGGTGGCCGCTGGCATCGGCATCGATCCCACCACCCGTTTCCTGATCACCACGCTACT
TGGCGGCATCGTGGCTTGCGTATTCGGCATTCTGATCGGTATCCCGGCGCTGCGTTTGCACGGCGACTACCTGGCCATCA
TCACGCTGGGCTTCGGCGAGATCATTCGCGTGATCATCCAGAACCTCAAGGTCGCCGGTGGAATGGGCCTCGACAAGGGC
GCCGCCGGACAGGCACTGATCGGCATTGACCGCACCGCCAACCTGTATGTCGTGTTCTGGATCATGGTGGTCACCGTGGT
CGTGCTCTTCATGTTCAGTCGCTCCCGGTACGGCCGTGCCGTCAAGGCCATTCGTGATGATGAGATCGCGGCCGGCGCAT
CGGGTCTGAACACCACGTATCTCAAGGTGTTGGTGTTCGCGATTTCCGCGTTCTTCGCAGGCATCGCCGGCGGTATCTTC
GCCCAGTACATCGGCTCGTTGAACCCGACCATGGCCGGCTGGCTCAACTCCATTAACTACGTGATCATGGTGGTGTTCGG
CGGCATGGGCTCGCTCACCGGTTCGATCGTTTCCGCCATCGGCCTGACCATCCTGCCCGAGCTGTTGCGTGCCTTCGCCG
ATTACCGCATGCTCGCCTACTCGGTGGTGCTGGTGCTGGTCATGATCTTCCGCCCGCAAGGCATCTTCGGTTCGTGGGAG
CTCTCGCTGCCGAGGCTGGTCAACCGCATGCTACTCCGCGGAACGGCTGGTAAAGGCGCAAAATCCGCCGCCAAGGCGGA
TGCCGAGCCGGAGACCCCCGAGACCGCAACGAAGGAGGCCTGA

Upstream 100 bases:

>100_bases
GCATTCTCCGATGCCATCGTGTTCGCCATCCTGATCATTGTGTTGCTCGTCAAACCGTCCGGCATCATGGGCAAGAACGA
AGGGGAGAAGGTCTGATCCG

Downstream 100 bases:

>100_bases
CCATGAGCGATAACAGTCAACCCATCCTCAAAGCCGAGCATCTCGGCATCACCTTCGGTGGTCTGAAGGCCGTATCCGAC
TTCAACATGACCATCAACAA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKKTMLSTKQSYLVCFIGVLAAFGVVMALCESGLADSYIKGIMMTACIAIIMTTSLNLTIGVLGQLTLGCCGFEAIGAYV
AALASKYMVAAGIGIDPTTRFLITTLLGGIVACVFGILIGIPALRLHGDYLAIITLGFGEIIRVIIQNLKVAGGMGLDKG
AAGQALIGIDRTANLYVVFWIMVVTVVVLFMFSRSRYGRAVKAIRDDEIAAGASGLNTTYLKVLVFAISAFFAGIAGGIF
AQYIGSLNPTMAGWLNSINYVIMVVFGGMGSLTGSIVSAIGLTILPELLRAFADYRMLAYSVVLVLVMIFRPQGIFGSWE
LSLPRLVNRMLLRGTAGKGAKSAAKADAEPETPETATKEA

Sequences:

>Translated_360_residues
MKKTMLSTKQSYLVCFIGVLAAFGVVMALCESGLADSYIKGIMMTACIAIIMTTSLNLTIGVLGQLTLGCCGFEAIGAYV
AALASKYMVAAGIGIDPTTRFLITTLLGGIVACVFGILIGIPALRLHGDYLAIITLGFGEIIRVIIQNLKVAGGMGLDKG
AAGQALIGIDRTANLYVVFWIMVVTVVVLFMFSRSRYGRAVKAIRDDEIAAGASGLNTTYLKVLVFAISAFFAGIAGGIF
AQYIGSLNPTMAGWLNSINYVIMVVFGGMGSLTGSIVSAIGLTILPELLRAFADYRMLAYSVVLVLVMIFRPQGIFGSWE
LSLPRLVNRMLLRGTAGKGAKSAAKADAEPETPETATKEA
>Mature_360_residues
MKKTMLSTKQSYLVCFIGVLAAFGVVMALCESGLADSYIKGIMMTACIAIIMTTSLNLTIGVLGQLTLGCCGFEAIGAYV
AALASKYMVAAGIGIDPTTRFLITTLLGGIVACVFGILIGIPALRLHGDYLAIITLGFGEIIRVIIQNLKVAGGMGLDKG
AAGQALIGIDRTANLYVVFWIMVVTVVVLFMFSRSRYGRAVKAIRDDEIAAGASGLNTTYLKVLVFAISAFFAGIAGGIF
AQYIGSLNPTMAGWLNSINYVIMVVFGGMGSLTGSIVSAIGLTILPELLRAFADYRMLAYSVVLVLVMIFRPQGIFGSWE
LSLPRLVNRMLLRGTAGKGAKSAAKADAEPETPETATKEA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=322, Percent_Identity=36.6459627329193, Blast_Score=148, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 37948; Mature: 37948

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTMLSTKQSYLVCFIGVLAAFGVVMALCESGLADSYIKGIMMTACIAIIMTTSLNLTI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
GVLGQLTLGCCGFEAIGAYVAALASKYMVAAGIGIDPTTRFLITTLLGGIVACVFGILIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
IPALRLHGDYLAIITLGFGEIIRVIIQNLKVAGGMGLDKGAAGQALIGIDRTANLYVVFW
CCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHH
IMVVTVVVLFMFSRSRYGRAVKAIRDDEIAAGASGLNTTYLKVLVFAISAFFAGIAGGIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQYIGSLNPTMAGWLNSINYVIMVVFGGMGSLTGSIVSAIGLTILPELLRAFADYRMLAY
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVVLVLVMIFRPQGIFGSWELSLPRLVNRMLLRGTAGKGAKSAAKADAEPETPETATKEA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKTMLSTKQSYLVCFIGVLAAFGVVMALCESGLADSYIKGIMMTACIAIIMTTSLNLTI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
GVLGQLTLGCCGFEAIGAYVAALASKYMVAAGIGIDPTTRFLITTLLGGIVACVFGILIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
IPALRLHGDYLAIITLGFGEIIRVIIQNLKVAGGMGLDKGAAGQALIGIDRTANLYVVFW
CCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHH
IMVVTVVVLFMFSRSRYGRAVKAIRDDEIAAGASGLNTTYLKVLVFAISAFFAGIAGGIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQYIGSLNPTMAGWLNSINYVIMVVFGGMGSLTGSIVSAIGLTILPELLRAFADYRMLAY
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVVLVLVMIFRPQGIFGSWELSLPRLVNRMLLRGTAGKGAKSAAKADAEPETPETATKEA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]