BacMap

Definition	Bifidobacterium longum subsp. infantis ATCC 15697, complete genome.
Accession	NC_011593
Length	2,832,748

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The map label for this gene is dkgB [H]

Identifier: 213691470

GI number: 213691470

Start: 643710

End: 644558

Strand: Direct

Name: dkgB [H]

Synonym: Blon_0567

Alternate gene names: 213691470

Gene position: 643710-644558 (Clockwise)

Preceding gene: 213691469

Following gene: 213691471

Centisome position: 22.72

GC content: 61.96

Gene sequence:

>849_bases
ATGCTTGAACCACCAGTCACTCCGATCCGCAAGGCCAACGACGGCCTCGAACTGCCCGCCACCGGCTTCGGTACGTACAA
GGTCAACGGTCTAGCCGGCGTGGACGCCATCAAGTTCGCCATCCATCAGGGTTACCGCCTCATCGACTCTGCGTTCAACT
ATGAGAACGAGGGTGTGGTGGGTCGCGCCATCCGCGAATCCGGCGTGCCGCGTGAAGACATCATCGTCACGTCCAAGCTG
CCCGGCCGCCACCATGCTCACGATGAGGCGCTGCTCACCATTGAGGAGAGCGTGTGCCGTATGGGTCTCGACTACATCGA
TCTCTATCTGATTCACTGGCCGAACCCAGGCCAAGGCCAGTTCGTCGAGGCATGGGAAGCGCTGGTCGAAGCTCGCGAAC
GCGGCTTGGTCAAGCACATTGGCGTCAGCAACTTCCTGCCCGGCCACATCGACCTGCTCATTCGCGATACGGGTGTGACG
CCGGCCGTCAACCAGGTCGAGCTGCACCCGTACTTCCAGCAGCGCGAACAGCTGGCCTACGACAACGCGCACGGCATCAT
CACCGAGGCATGGAGCCCGCTGGGCCGCGCCAACCAGATGCTGCGCGACCCAGACCTGAAGCGTATCGCCGCCAAGCACG
GTATTTCCGTGGTCCAGGCCATTTTGCGCTGGCACCTCCAGTACGGCGACGTGGCCATCCCGAAGTCTTTGAACCCGGAG
CGCCAGCGTCAGAACCTCGACCTGACCGCTTTTGAACTCGATGATCAGGACATGGCCGATATCGCCGCGATGGACAACCC
CGAAGGCCACACCTTCGGACAGAACCCCCACTGGCACGAAGAAGCGTGA

Upstream 100 bases:

>100_bases
ACCGCCAGAATTGAGAGAAGTCAAATAGTACGCCCGCGGCGGATTGGTTGGCTTTATGATGGAACCGACATTCCATCACG
GGAGAAGCAGGGAAACTATC

Downstream 100 bases:

>100_bases
TTCCAACATGCAACGTCCTATTATGACTTCGCGGTCGTTTTTGCGCACGCCGTCCGAAGTGGCCGGACCCGGCGACGAGC
AGGTGATCGAAGACCTCGTA

Product: 2,5-didehydrogluconate reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MLEPPVTPIRKANDGLELPATGFGTYKVNGLAGVDAIKFAIHQGYRLIDSAFNYENEGVVGRAIRESGVPREDIIVTSKL
PGRHHAHDEALLTIEESVCRMGLDYIDLYLIHWPNPGQGQFVEAWEALVEARERGLVKHIGVSNFLPGHIDLLIRDTGVT
PAVNQVELHPYFQQREQLAYDNAHGIITEAWSPLGRANQMLRDPDLKRIAAKHGISVVQAILRWHLQYGDVAIPKSLNPE
RQRQNLDLTAFELDDQDMADIAAMDNPEGHTFGQNPHWHEEA

Sequences:

>Translated_282_residues
MLEPPVTPIRKANDGLELPATGFGTYKVNGLAGVDAIKFAIHQGYRLIDSAFNYENEGVVGRAIRESGVPREDIIVTSKL
PGRHHAHDEALLTIEESVCRMGLDYIDLYLIHWPNPGQGQFVEAWEALVEARERGLVKHIGVSNFLPGHIDLLIRDTGVT
PAVNQVELHPYFQQREQLAYDNAHGIITEAWSPLGRANQMLRDPDLKRIAAKHGISVVQAILRWHLQYGDVAIPKSLNPE
RQRQNLDLTAFELDDQDMADIAAMDNPEGHTFGQNPHWHEEA
>Mature_282_residues
MLEPPVTPIRKANDGLELPATGFGTYKVNGLAGVDAIKFAIHQGYRLIDSAFNYENEGVVGRAIRESGVPREDIIVTSKL
PGRHHAHDEALLTIEESVCRMGLDYIDLYLIHWPNPGQGQFVEAWEALVEARERGLVKHIGVSNFLPGHIDLLIRDTGVT
PAVNQVELHPYFQQREQLAYDNAHGIITEAWSPLGRANQMLRDPDLKRIAAKHGISVVQAILRWHLQYGDVAIPKSLNPE
RQRQNLDLTAFELDDQDMADIAAMDNPEGHTFGQNPHWHEEA

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 25DKGR-B has higher catalytic efficiency than 25DKGR-A [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=282, Percent_Identity=36.1702127659575, Blast_Score=177, Evalue=9e-45,
Organism=Homo sapiens, GI24497577, Length=284, Percent_Identity=34.8591549295775, Blast_Score=167, Evalue=7e-42,
Organism=Homo sapiens, GI5174391, Length=284, Percent_Identity=34.8591549295775, Blast_Score=167, Evalue=7e-42,
Organism=Homo sapiens, GI291291012, Length=302, Percent_Identity=34.7682119205298, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI5453543, Length=292, Percent_Identity=36.6438356164384, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI310109920, Length=292, Percent_Identity=36.6438356164384, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI5174695, Length=291, Percent_Identity=34.7079037800687, Blast_Score=164, Evalue=7e-41,
Organism=Homo sapiens, GI45446745, Length=292, Percent_Identity=36.3013698630137, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4503285, Length=292, Percent_Identity=36.3013698630137, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI310109922, Length=274, Percent_Identity=37.5912408759124, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI24497583, Length=309, Percent_Identity=34.9514563106796, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI4502049, Length=286, Percent_Identity=36.013986013986, Blast_Score=160, Evalue=9e-40,
Organism=Homo sapiens, GI24497585, Length=283, Percent_Identity=35.6890459363958, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI93277124, Length=295, Percent_Identity=30.5084745762712, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI300116273, Length=274, Percent_Identity=34.6715328467153, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI300116271, Length=275, Percent_Identity=32, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI310109924, Length=148, Percent_Identity=34.4594594594595, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI310109926, Length=111, Percent_Identity=45.945945945946, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI207028673, Length=111, Percent_Identity=45.045045045045, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI87082198, Length=268, Percent_Identity=42.1641791044776, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI1786400, Length=271, Percent_Identity=36.5313653136531, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788081, Length=282, Percent_Identity=30.4964539007092, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17564128, Length=291, Percent_Identity=34.3642611683849, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17552492, Length=263, Percent_Identity=33.4600760456274, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17561298, Length=271, Percent_Identity=33.9483394833948, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17538386, Length=274, Percent_Identity=34.3065693430657, Blast_Score=143, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17537079, Length=298, Percent_Identity=29.5302013422819, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=33.6805555555556, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17537075, Length=287, Percent_Identity=33.4494773519164, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71998625, Length=268, Percent_Identity=32.4626865671642, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=32.1033210332103, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17566692, Length=283, Percent_Identity=30.3886925795053, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550248, Length=289, Percent_Identity=29.4117647058824, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17562292, Length=286, Percent_Identity=27.2727272727273, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320576, Length=288, Percent_Identity=37.5, Blast_Score=185, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6324694, Length=301, Percent_Identity=35.5481727574751, Blast_Score=183, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321896, Length=300, Percent_Identity=34.3333333333333, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322556, Length=274, Percent_Identity=32.8467153284672, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319625, Length=300, Percent_Identity=32.6666666666667, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6320079, Length=230, Percent_Identity=29.5652173913043, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24657054, Length=288, Percent_Identity=34.0277777777778, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24644950, Length=292, Percent_Identity=34.2465753424658, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI20129731, Length=287, Percent_Identity=35.5400696864112, Blast_Score=158, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21356425, Length=291, Percent_Identity=32.3024054982818, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI221378297, Length=312, Percent_Identity=32.3717948717949, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24663317, Length=284, Percent_Identity=32.7464788732394, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24662789, Length=295, Percent_Identity=31.864406779661, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45553081, Length=290, Percent_Identity=29.6551724137931, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24662781, Length=277, Percent_Identity=34.6570397111913, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24662785, Length=271, Percent_Identity=34.6863468634686, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI281366140, Length=252, Percent_Identity=29.3650793650794, Blast_Score=111, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 31589; Mature: 31589

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEPPVTPIRKANDGLELPATGFGTYKVNGLAGVDAIKFAIHQGYRLIDSAFNYENEGVV
CCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCHHHHHHHHCCCCCCHH
GRAIRESGVPREDIIVTSKLPGRHHAHDEALLTIEESVCRMGLDYIDLYLIHWPNPGQGQ
HHHHHHCCCCHHHEEEECCCCCCCCCCCHHEEHHHHHHHHHCCCEEEEEEEECCCCCCCH
FVEAWEALVEARERGLVKHIGVSNFLPGHIDLLIRDTGVTPAVNQVELHPYFQQREQLAY
HHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEECHHHHHHHHHHH
DNAHGIITEAWSPLGRANQMLRDPDLKRIAAKHGISVVQAILRWHLQYGDVAIPKSLNPE
CCCCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHH
RQRQNLDLTAFELDDQDMADIAAMDNPEGHTFGQNPHWHEEA
HHHCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLEPPVTPIRKANDGLELPATGFGTYKVNGLAGVDAIKFAIHQGYRLIDSAFNYENEGVV
CCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCHHHHHHHHCCCCCCHH
GRAIRESGVPREDIIVTSKLPGRHHAHDEALLTIEESVCRMGLDYIDLYLIHWPNPGQGQ
HHHHHHCCCCHHHEEEECCCCCCCCCCCHHEEHHHHHHHHHCCCEEEEEEEECCCCCCCH
FVEAWEALVEARERGLVKHIGVSNFLPGHIDLLIRDTGVTPAVNQVELHPYFQQREQLAY
HHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEECHHHHHHHHHHH
DNAHGIITEAWSPLGRANQMLRDPDLKRIAAKHGISVVQAILRWHLQYGDVAIPKSLNPE
CCCCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHH
RQRQNLDLTAFELDDQDMADIAAMDNPEGHTFGQNPHWHEEA
HHHCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA