Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 213691471

GI number: 213691471

Start: 644566

End: 644985

Strand: Direct

Name: def [H]

Synonym: Blon_0568

Alternate gene names: 213691471

Gene position: 644566-644985 (Clockwise)

Preceding gene: 213691470

Following gene: 213691472

Centisome position: 22.75

GC content: 60.24

Gene sequence:

>420_bases
ATGCAACGTCCTATTATGACTTCGCGGTCGTTTTTGCGCACGCCGTCCGAAGTGGCCGGACCCGGCGACGAGCAGGTGAT
CGAAGACCTCGTAGACACGCTGGAGGCCAACCGTTCACGGTGCGTGGGCATGGCCGCCAACATGATTGGCGTAGGCAAAC
GCATCATCGTCTTCGTGGATGAAGACCTTGGCGGGCGTATTACGGTGATGTTCAACCCCGTGATCACCGCTTCGGACGGT
GCCTTCGACGCCCAGGAAGGCTGTTTGTCGCTGACCGGCGAACGCCGCACACTGCGCTACCGCCGTATCGAGGTCAACTA
CGAGGACCGTCGGTTCCGCGCCCGCCACGCCACCTTCGCCGGTTGGACTGCGCAGATTATCCAGCATGAAGTCGATCACT
GCAACGGCATCATTATCTGA

Upstream 100 bases:

>100_bases
CTCGATGATCAGGACATGGCCGATATCGCCGCGATGGACAACCCCGAAGGCCACACCTTCGGACAGAACCCCCACTGGCA
CGAAGAAGCGTGATTCCAAC

Downstream 100 bases:

>100_bases
TTCATATCGGTGCTCGTTGCAGCCCAACCCCCGAGGCTGGTCATCGCGATTACGATGCGATTACATCGCAACCGCTATGA
CCAGCCTTTTTTTATGTGCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MQRPIMTSRSFLRTPSEVAGPGDEQVIEDLVDTLEANRSRCVGMAANMIGVGKRIIVFVDEDLGGRITVMFNPVITASDG
AFDAQEGCLSLTGERRTLRYRRIEVNYEDRRFRARHATFAGWTAQIIQHEVDHCNGIII

Sequences:

>Translated_139_residues
MQRPIMTSRSFLRTPSEVAGPGDEQVIEDLVDTLEANRSRCVGMAANMIGVGKRIIVFVDEDLGGRITVMFNPVITASDG
AFDAQEGCLSLTGERRTLRYRRIEVNYEDRRFRARHATFAGWTAQIIQHEVDHCNGIII
>Mature_139_residues
MQRPIMTSRSFLRTPSEVAGPGDEQVIEDLVDTLEANRSRCVGMAANMIGVGKRIIVFVDEDLGGRITVMFNPVITASDG
AFDAQEGCLSLTGERRTLRYRRIEVNYEDRRFRARHATFAGWTAQIIQHEVDHCNGIII

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 15597; Mature: 15597

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRPIMTSRSFLRTPSEVAGPGDEQVIEDLVDTLEANRSRCVGMAANMIGVGKRIIVFVD
CCCCCCCCHHHHCCCHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEE
EDLGGRITVMFNPVITASDGAFDAQEGCLSLTGERRTLRYRRIEVNYEDRRFRARHATFA
CCCCCEEEEEECCEEEECCCCCCCCCCCEECCCCCCEEEEEEEEECCCHHHHHHHHCCCC
GWTAQIIQHEVDHCNGIII
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQRPIMTSRSFLRTPSEVAGPGDEQVIEDLVDTLEANRSRCVGMAANMIGVGKRIIVFVD
CCCCCCCCHHHHCCCHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEE
EDLGGRITVMFNPVITASDGAFDAQEGCLSLTGERRTLRYRRIEVNYEDRRFRARHATFA
CCCCCEEEEEECCEEEECCCCCCCCCCCEECCCCCCEEEEEEEEECCCHHHHHHHHCCCC
GWTAQIIQHEVDHCNGIII
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9572951 [H]