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The map label for this gene is glkA [H]

Identifier: 213691469

GI number: 213691469

Start: 642020

End: 642970

Strand: Direct

Name: glkA [H]

Synonym: Blon_0565

Alternate gene names: 213691469

Gene position: 642020-642970 (Clockwise)

Preceding gene: 213691468

Following gene: 213691470

Centisome position: 22.66

GC content: 64.67

Gene sequence:

>951_bases
ATGACCACGCTCGCCATTGATATCGGCGGAACCAAGATCGCCGCTGCCGTCTGCGATGCAAACGACAGCATCATCCAGCG
TTGGCGCGTCCCCACGCCGATGGACGCCGACGCCATCAACACGCATATCGCCGAGATCTACCGCGAAGCCGTCGCCGCTG
GCCACACCGATATCGAGGCCATCGGCATCTCCGCGGCCGGTAATGTGAGCGCGGATCGCAAGACGCTCACCTTCTCCGCC
AACATTCCGGCCTGGATCAATTACAATCTGTCCGAGCATGTCGGCGCGTTGATCGACCATGCCGTTCCGATCGTCGTCGA
GAACGATGCCAACTGCGCCGGCTGGGGCGAATTCGTGCACGGTGCCGGCCAAGGCAGCTCCAACATGGTCGCGCTGACCG
TGGGCACCGGCCTCGGTGGCGCCATCGTGATCAATGGCCAGCTCTACCGAGGCTCCTTCGGCATGGCCGCAGAGCTCGGC
CACCTGCCGATGGTCCCGGACGGAGACCATTGCGGCTGCGGCCTGCGCGGCTGCGCCGAGCGCTATACTTCGGGAACCTC
GCTGGAGAACTTCGCCAAGTCCGCCGTGCGTCGTCGTCCTCAGGACGCCAAACGTCTTCTGGAGCTGTGCGGTGGCGACA
TCACCAAGCTGGAGGGACCGATGGTGTCCCAAGCGGCTCAGGAGGGTGACGTGCTTGGCCTGTATGCGTTCGGCAAGATC
GGCGAATGGCTTGGCCGCACCATGGCCGCCGTTTCCGCCGTGCTTGATCCGGACCTGTTCGTCATCGGCGGTGGTGTGGT
AGCCGTTGGAGACATTCTGCTGGAGCCGGCCCGCTACAACTACCAGCGTTTCCTGGAAGGCAGCGCCTACCGTGGCCACG
CCAAGATCGTCGCCGCAGTCGCGGGCCAGGACGCCGGCCTCATCGGTGCCGCCAATCTGGCTCTTCGCTGA

Upstream 100 bases:

>100_bases
GACGCATGGCGAATCACGGAAAATCCGCGGTGGGAGGTCATGGCGGCTCACTACTATTTTCGCTATCTCGTACGTTACGA
AAGTCTTCAAGGAGTAAGCC

Downstream 100 bases:

>100_bases
CGCAGATGGCGGTGCGTAGATAACGCGCGCGACGCAGATAGCAACGCGTAAATAATCGCAAATAATCGCAAACAGTACAG
TGCGGTGATTCTCGTTATGG

Product: glucokinase, ROK family

Products: NA

Alternate protein names: Glucose kinase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTTLAIDIGGTKIAAAVCDANDSIIQRWRVPTPMDADAINTHIAEIYREAVAAGHTDIEAIGISAAGNVSADRKTLTFSA
NIPAWINYNLSEHVGALIDHAVPIVVENDANCAGWGEFVHGAGQGSSNMVALTVGTGLGGAIVINGQLYRGSFGMAAELG
HLPMVPDGDHCGCGLRGCAERYTSGTSLENFAKSAVRRRPQDAKRLLELCGGDITKLEGPMVSQAAQEGDVLGLYAFGKI
GEWLGRTMAAVSAVLDPDLFVIGGGVVAVGDILLEPARYNYQRFLEGSAYRGHAKIVAAVAGQDAGLIGAANLALR

Sequences:

>Translated_316_residues
MTTLAIDIGGTKIAAAVCDANDSIIQRWRVPTPMDADAINTHIAEIYREAVAAGHTDIEAIGISAAGNVSADRKTLTFSA
NIPAWINYNLSEHVGALIDHAVPIVVENDANCAGWGEFVHGAGQGSSNMVALTVGTGLGGAIVINGQLYRGSFGMAAELG
HLPMVPDGDHCGCGLRGCAERYTSGTSLENFAKSAVRRRPQDAKRLLELCGGDITKLEGPMVSQAAQEGDVLGLYAFGKI
GEWLGRTMAAVSAVLDPDLFVIGGGVVAVGDILLEPARYNYQRFLEGSAYRGHAKIVAAVAGQDAGLIGAANLALR
>Mature_315_residues
TTLAIDIGGTKIAAAVCDANDSIIQRWRVPTPMDADAINTHIAEIYREAVAAGHTDIEAIGISAAGNVSADRKTLTFSAN
IPAWINYNLSEHVGALIDHAVPIVVENDANCAGWGEFVHGAGQGSSNMVALTVGTGLGGAIVINGQLYRGSFGMAAELGH
LPMVPDGDHCGCGLRGCAERYTSGTSLENFAKSAVRRRPQDAKRLLELCGGDITKLEGPMVSQAAQEGDVLGLYAFGKIG
EWLGRTMAAVSAVLDPDLFVIGGGVVAVGDILLEPARYNYQRFLEGSAYRGHAKIVAAVAGQDAGLIGAANLALR

Specific function: Required for glucose repression of many different genes [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566315, Length=271, Percent_Identity=30.6273062730627, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4885285, Length=271, Percent_Identity=30.6273062730627, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI298566325, Length=271, Percent_Identity=30.6273062730627, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI190014632, Length=271, Percent_Identity=30.6273062730627, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1787363, Length=276, Percent_Identity=27.8985507246377, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081733, Length=323, Percent_Identity=30.030959752322, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082230, Length=270, Percent_Identity=30.7407407407407, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790522, Length=249, Percent_Identity=26.5060240963855, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786891, Length=223, Percent_Identity=26.0089686098655, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787878, Length=222, Percent_Identity=25.2252252252252, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR004654 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 32856; Mature: 32724

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLAIDIGGTKIAAAVCDANDSIIQRWRVPTPMDADAINTHIAEIYREAVAAGHTDIEA
CEEEEEEECCCEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
IGISAAGNVSADRKTLTFSANIPAWINYNLSEHVGALIDHAVPIVVENDANCAGWGEFVH
EEEECCCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHH
GAGQGSSNMVALTVGTGLGGAIVINGQLYRGSFGMAAELGHLPMVPDGDHCGCGLRGCAE
CCCCCCCCEEEEEECCCCCCEEEECCEEEECCCCCHHHCCCCCCCCCCCCCCCCHHHHHH
RYTSGTSLENFAKSAVRRRPQDAKRLLELCGGDITKLEGPMVSQAAQEGDVLGLYAFGKI
HHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCHHHHHHHCCCEEEEEEHHHH
GEWLGRTMAAVSAVLDPDLFVIGGGVVAVGDILLEPARYNYQRFLEGSAYRGHAKIVAAV
HHHHHHHHHHHHHHCCCCEEEECCCHHEEHHHHHCCCCCCHHHHHCCCCCCCCEEEEEEE
AGQDAGLIGAANLALR
CCCCCCEEECCCEECC
>Mature Secondary Structure 
TTLAIDIGGTKIAAAVCDANDSIIQRWRVPTPMDADAINTHIAEIYREAVAAGHTDIEA
EEEEEEECCCEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
IGISAAGNVSADRKTLTFSANIPAWINYNLSEHVGALIDHAVPIVVENDANCAGWGEFVH
EEEECCCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHH
GAGQGSSNMVALTVGTGLGGAIVINGQLYRGSFGMAAELGHLPMVPDGDHCGCGLRGCAE
CCCCCCCCEEEEEECCCCCCEEEECCEEEECCCCCHHHCCCCCCCCCCCCCCCCHHHHHH
RYTSGTSLENFAKSAVRRRPQDAKRLLELCGGDITKLEGPMVSQAAQEGDVLGLYAFGKI
HHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCHHHHHHHCCCEEEEEEHHHH
GEWLGRTMAAVSAVLDPDLFVIGGGVVAVGDILLEPARYNYQRFLEGSAYRGHAKIVAAV
HHHHHHHHHHHHHHCCCCEEEECCCHHEEHHHHHCCCCCCHHHHHCCCCCCCCEEEEEEE
AGQDAGLIGAANLALR
CCCCCCEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1435260; 12000953 [H]