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The map label for this gene is lacI [H]

Identifier: 213691336

GI number: 213691336

Start: 488482

End: 489597

Strand: Direct

Name: lacI [H]

Synonym: Blon_0423

Alternate gene names: 213691336

Gene position: 488482-489597 (Clockwise)

Preceding gene: 213691335

Following gene: 213691337

Centisome position: 17.24

GC content: 62.81

Gene sequence:

>1116_bases
ATGGTGACCGGCCGCAAGCCAAGCAAACGTCCGTCCATGTTCGAAGTGGCCAAGCTCGCCGGAGTCAGCCACCAAACCGT
CTCCCGTGTGATCAATGACTCGCCGGACGTTTCCGACGCCACCCGTGCGCGAGTCCAGGCCGCCATTGACCAACTCGGCT
ACCGCCCATCCAATTCCGCGCGCGCACTGGCTTCCCGCCGTTCGCGCACCATAGGCCTGATCGCCGGAGGCCTCAAATTC
TTCGGCCCGATTTCCTCCATCTCCTCCATCGAATCCATGGCCCGCGAGCACGGTCTGTTCATGAGCGTGATGATGGTCCA
CGAGGCTTTGTGCACACAGGTCGACTTCGACAACCTGTGCTACACCTTCAACGAGCAGAACGTCGACGCCTTCATCTTCC
TGACTCCGACCGACGTGATGTTCGCCGCCGCCTGCCGTGCGCGAGTCACTCAGCCGCGCGTCATCGTCACCGCCACCCAC
GGCCAAATGACCGTGCGTGAGGGATTGGGCCTTATCTCCACCGAAAACAAGCGTCGCACTGCGCTGGTCGGTATCGACCA
GTGGGGTGCAATGGCCAAAGTGGTGGCCTTGCTGGGCGAATACGGGCACAAATCGGCTCTGTATTTTGCCGGTCCCAACG
AATGGCGCGACGCCCACACCCGACTGGATGCGTGGCGCAAGCTCGCCGCCTCCCGATCCATCGATTCACAGATCGTGCGC
TGCCATACGTGGGATGCCTCTGAGGCATATGCGCACATGAATCATCTCATTGATGAGTATGGGCGTCGGGGCATGGCGTT
ACCCACTGCGGTCGTCGCCGCGAACGACAATCAGGCCGTCGGCGTCATGCGCGCGTTGCATGAGCATGGGCTTCGGATTC
CGCAAGACATCAGCGTGGTCGGATTCGACGACATGTCTGGCGTGGATAACATGTACCCACCGCTGACCACCGTGCATCCC
GATTTCGAGGGGCTTGGCGTGGCCGCCATGCGCGAGACGCTGCGTTTGCTGGGGGAGGGCGGCGAGCCGACCTTCCTGAC
CACCCAGCACGGCGTGGGCCTGATCCCCGCCGAAGTGCGTGCTCGGCGGTCCTTGGGACCGGCTCCCCGGCGGTAA

Upstream 100 bases:

>100_bases
GCTAACGCAAAACTGTTGTGGACGCGCTAAAGTCAGTATTGATTAAGGTACAAGACTGGCCGCGCATGGGTGCCGGCCGC
CAGCGGGGAGGTTAGGCAGC

Downstream 100 bases:

>100_bases
GGCTGGGCTTAGCGGGGCGCTTCTTCGGATGATTCGAGGGTTTGCTTCGCTCCCCGGTCAAGATTGGCATGGACGTGATG
ACCAAGCAGGAGCACGTGCA

Product: regulatory protein, LacI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MVTGRKPSKRPSMFEVAKLAGVSHQTVSRVINDSPDVSDATRARVQAAIDQLGYRPSNSARALASRRSRTIGLIAGGLKF
FGPISSISSIESMAREHGLFMSVMMVHEALCTQVDFDNLCYTFNEQNVDAFIFLTPTDVMFAAACRARVTQPRVIVTATH
GQMTVREGLGLISTENKRRTALVGIDQWGAMAKVVALLGEYGHKSALYFAGPNEWRDAHTRLDAWRKLAASRSIDSQIVR
CHTWDASEAYAHMNHLIDEYGRRGMALPTAVVAANDNQAVGVMRALHEHGLRIPQDISVVGFDDMSGVDNMYPPLTTVHP
DFEGLGVAAMRETLRLLGEGGEPTFLTTQHGVGLIPAEVRARRSLGPAPRR

Sequences:

>Translated_371_residues
MVTGRKPSKRPSMFEVAKLAGVSHQTVSRVINDSPDVSDATRARVQAAIDQLGYRPSNSARALASRRSRTIGLIAGGLKF
FGPISSISSIESMAREHGLFMSVMMVHEALCTQVDFDNLCYTFNEQNVDAFIFLTPTDVMFAAACRARVTQPRVIVTATH
GQMTVREGLGLISTENKRRTALVGIDQWGAMAKVVALLGEYGHKSALYFAGPNEWRDAHTRLDAWRKLAASRSIDSQIVR
CHTWDASEAYAHMNHLIDEYGRRGMALPTAVVAANDNQAVGVMRALHEHGLRIPQDISVVGFDDMSGVDNMYPPLTTVHP
DFEGLGVAAMRETLRLLGEGGEPTFLTTQHGVGLIPAEVRARRSLGPAPRR
>Mature_371_residues
MVTGRKPSKRPSMFEVAKLAGVSHQTVSRVINDSPDVSDATRARVQAAIDQLGYRPSNSARALASRRSRTIGLIAGGLKF
FGPISSISSIESMAREHGLFMSVMMVHEALCTQVDFDNLCYTFNEQNVDAFIFLTPTDVMFAAACRARVTQPRVIVTATH
GQMTVREGLGLISTENKRRTALVGIDQWGAMAKVVALLGEYGHKSALYFAGPNEWRDAHTRLDAWRKLAASRSIDSQIVR
CHTWDASEAYAHMNHLIDEYGRRGMALPTAVVAANDNQAVGVMRALHEHGLRIPQDISVVGFDDMSGVDNMYPPLTTVHP
DFEGLGVAAMRETLRLLGEGGEPTFLTTQHGVGLIPAEVRARRSLGPAPRR

Specific function: Repressor of the lactose operon. Binds allolactose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786540, Length=332, Percent_Identity=32.2289156626506, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789202, Length=317, Percent_Identity=30.5993690851735, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789068, Length=318, Percent_Identity=26.4150943396226, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790194, Length=324, Percent_Identity=30.8641975308642, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=28.9940828402367, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=28.8235294117647, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787580, Length=348, Percent_Identity=24.4252873563218, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994940, Length=350, Percent_Identity=25.7142857142857, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 40580; Mature: 40580

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTGRKPSKRPSMFEVAKLAGVSHQTVSRVINDSPDVSDATRARVQAAIDQLGYRPSNSA
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
RALASRRSRTIGLIAGGLKFFGPISSISSIESMAREHGLFMSVMMVHEALCTQVDFDNLC
HHHHHHCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YTFNEQNVDAFIFLTPTDVMFAAACRARVTQPRVIVTATHGQMTVREGLGLISTENKRRT
EEECCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHCCCEEECCCCCEE
ALVGIDQWGAMAKVVALLGEYGHKSALYFAGPNEWRDAHTRLDAWRKLAASRSIDSQIVR
EEEECHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHEEE
CHTWDASEAYAHMNHLIDEYGRRGMALPTAVVAANDNQAVGVMRALHEHGLRIPQDISVV
EECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEE
GFDDMSGVDNMYPPLTTVHPDFEGLGVAAMRETLRLLGEGGEPTFLTTQHGVGLIPAEVR
ECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHH
ARRSLGPAPRR
HHHCCCCCCCC
>Mature Secondary Structure
MVTGRKPSKRPSMFEVAKLAGVSHQTVSRVINDSPDVSDATRARVQAAIDQLGYRPSNSA
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
RALASRRSRTIGLIAGGLKFFGPISSISSIESMAREHGLFMSVMMVHEALCTQVDFDNLC
HHHHHHCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YTFNEQNVDAFIFLTPTDVMFAAACRARVTQPRVIVTATHGQMTVREGLGLISTENKRRT
EEECCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHCCCEEECCCCCEE
ALVGIDQWGAMAKVVALLGEYGHKSALYFAGPNEWRDAHTRLDAWRKLAASRSIDSQIVR
EEEECHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHEEE
CHTWDASEAYAHMNHLIDEYGRRGMALPTAVVAANDNQAVGVMRALHEHGLRIPQDISVV
EECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEE
GFDDMSGVDNMYPPLTTVHPDFEGLGVAAMRETLRLLGEGGEPTFLTTQHGVGLIPAEVR
ECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHH
ARRSLGPAPRR
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 355891; 9278503; 1107032; 4571224; 4594037; 3286877; 7498473; 2178920; 8046748; 2040302; 1923807; 3064080; 2742823; 8683581; 10647179; 8638105 [H]