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The map label for this gene is rihA [H]

Identifier: 213691286

GI number: 213691286

Start: 432788

End: 433843

Strand: Direct

Name: rihA [H]

Synonym: Blon_0373

Alternate gene names: 213691286

Gene position: 432788-433843 (Clockwise)

Preceding gene: 213691285

Following gene: 213691287

Centisome position: 15.28

GC content: 62.31

Gene sequence:

>1056_bases
ATGCGTAAAAAACTCATCCTCGACCTCGATACCGGCATCGATGACGCGCTCGCCATCGCATATGCCCTAGGCAGTGCCGA
CGAGATCGATCTGATCGGTATCACGGCCACCTACGGCAATGTCGCGGTGCCCCTGGCCACGCGCAACGCGCTGGCGGTAC
TGCACTTGTTCGGGCGTGACGATGTCCCGGTCTATCCCGGAGTCGACTATCCCATCGCCCCGGCAACCTTGCCGACAATG
CGTGGCACCACACCGGATGAGCGGGCCCAAGCCTATGCGCGCTGGCAGAACGGCGGCGCGGGCGGCTGGCAAACGGACGC
TCCGTGGTCACCAACTCCCGGCTCGGTGGCGGTCCACCATCCCAACGGCATCGGTGGCGCAATCATCCCCGACTCCCCGC
GCGCTCCGGAAGCACCAGGCTCGGCGGTCGATTTCATCATCACCGCCGCGCACGAATACGGCCCGGATCTGACCATCGTA
CCTACGGGCGCGATGACCACCATGGCCACCGTGTTCCGTAATGCCCCGGATCTGAAGGATTCCGGCGTGAACGTGACGTT
GATGGGTGGTTCGCTGACACTACCGGGCAATGTAAGTCCTGCTGCCGAAGCGAACATCTCCCAGGATCCCGAGGCCGCTG
ATTACCTGTTCAAGTGCGGTGCCCGCACCACGATGATTGGCCTCGACGTGACTCATCAGACCGTGCTCACCCGCGAGAAA
GCTCATGAATGGGACGCTCTGGGAACGCCGGCCGGTGACTTCCTCGTCACCATGACCGATTACTACATCGATTTCTACGT
AAAGAACCAGCCGGAAATCCACGGCTGCGGACTGCATGACCCGCTCGCTGTGGCTGCCGCGCTTGACCCATCATTGATCA
CCACGTTCGGCTTCAACCTACAGGTCGATTTGGAGGGCCCGTTCCGTGGCCGCACCATTGGTGACCGCTCGCGGCTGCAA
GATTTCGACAAGCACACTCAGGTTGCGCTCGGCGTCGACGTGTCGGAATTCCTCGACCGCTTTATGGCTCGCGTCACCGC
CGCGGCTCGTGGGTAA

Upstream 100 bases:

>100_bases
AAGCTCGGCTGAACTTTCTCAGCTCCCCCTTGTCAGGGGAGCTGTCTCGCGTAGCGAGACTGAGGGGTAGTCGCTGGAGT
AGTCCCGCAAGGAGATAATT

Downstream 100 bases:

>100_bases
ACGCTTATGCTTTGGCGGTCGTGTCGGTTGCTGAAGCGTCGGTTAGTAGTGCGGACAATGCGGTTCTAGCTTCCGGGGTG
ATCCACATGGTGTGTGGATC

Product: Inosine/uridine-preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MRKKLILDLDTGIDDALAIAYALGSADEIDLIGITATYGNVAVPLATRNALAVLHLFGRDDVPVYPGVDYPIAPATLPTM
RGTTPDERAQAYARWQNGGAGGWQTDAPWSPTPGSVAVHHPNGIGGAIIPDSPRAPEAPGSAVDFIITAAHEYGPDLTIV
PTGAMTTMATVFRNAPDLKDSGVNVTLMGGSLTLPGNVSPAAEANISQDPEAADYLFKCGARTTMIGLDVTHQTVLTREK
AHEWDALGTPAGDFLVTMTDYYIDFYVKNQPEIHGCGLHDPLAVAAALDPSLITTFGFNLQVDLEGPFRGRTIGDRSRLQ
DFDKHTQVALGVDVSEFLDRFMARVTAAARG

Sequences:

>Translated_351_residues
MRKKLILDLDTGIDDALAIAYALGSADEIDLIGITATYGNVAVPLATRNALAVLHLFGRDDVPVYPGVDYPIAPATLPTM
RGTTPDERAQAYARWQNGGAGGWQTDAPWSPTPGSVAVHHPNGIGGAIIPDSPRAPEAPGSAVDFIITAAHEYGPDLTIV
PTGAMTTMATVFRNAPDLKDSGVNVTLMGGSLTLPGNVSPAAEANISQDPEAADYLFKCGARTTMIGLDVTHQTVLTREK
AHEWDALGTPAGDFLVTMTDYYIDFYVKNQPEIHGCGLHDPLAVAAALDPSLITTFGFNLQVDLEGPFRGRTIGDRSRLQ
DFDKHTQVALGVDVSEFLDRFMARVTAAARG
>Mature_351_residues
MRKKLILDLDTGIDDALAIAYALGSADEIDLIGITATYGNVAVPLATRNALAVLHLFGRDDVPVYPGVDYPIAPATLPTM
RGTTPDERAQAYARWQNGGAGGWQTDAPWSPTPGSVAVHHPNGIGGAIIPDSPRAPEAPGSAVDFIITAAHEYGPDLTIV
PTGAMTTMATVFRNAPDLKDSGVNVTLMGGSLTLPGNVSPAAEANISQDPEAADYLFKCGARTTMIGLDVTHQTVLTREK
AHEWDALGTPAGDFLVTMTDYYIDFYVKNQPEIHGCGLHDPLAVAAALDPSLITTFGFNLQVDLEGPFRGRTIGDRSRLQ
DFDKHTQVALGVDVSEFLDRFMARVTAAARG

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=346, Percent_Identity=30.3468208092486, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788486, Length=354, Percent_Identity=30.225988700565, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1786213, Length=351, Percent_Identity=31.3390313390313, Blast_Score=95, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320608, Length=367, Percent_Identity=25.3405994550409, Blast_Score=92, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 37252; Mature: 37252

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKLILDLDTGIDDALAIAYALGSADEIDLIGITATYGNVAVPLATRNALAVLHLFGRD
CCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEECCCEEEEEECCCEEEEEEEECCC
DVPVYPGVDYPIAPATLPTMRGTTPDERAQAYARWQNGGAGGWQTDAPWSPTPGSVAVHH
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEC
PNGIGGAIIPDSPRAPEAPGSAVDFIITAAHEYGPDLTIVPTGAMTTMATVFRNAPDLKD
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCEEEEECCHHHHHHHHHHCCCCCCC
SGVNVTLMGGSLTLPGNVSPAAEANISQDPEAADYLFKCGARTTMIGLDVTHQTVLTREK
CCCEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
AHEWDALGTPAGDFLVTMTDYYIDFYVKNQPEIHGCGLHDPLAVAAALDPSLITTFGFNL
HHCCCCCCCCCCCEEEEEEEEEEEEEECCCCCEECCCCCCHHHHHHHCCCHHHEEECCEE
QVDLEGPFRGRTIGDRSRLQDFDKHTQVALGVDVSEFLDRFMARVTAAARG
EEEECCCCCCCCCCCHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKKLILDLDTGIDDALAIAYALGSADEIDLIGITATYGNVAVPLATRNALAVLHLFGRD
CCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEECCCEEEEEECCCEEEEEEEECCC
DVPVYPGVDYPIAPATLPTMRGTTPDERAQAYARWQNGGAGGWQTDAPWSPTPGSVAVHH
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEC
PNGIGGAIIPDSPRAPEAPGSAVDFIITAAHEYGPDLTIVPTGAMTTMATVFRNAPDLKD
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCEEEEECCHHHHHHHHHHCCCCCCC
SGVNVTLMGGSLTLPGNVSPAAEANISQDPEAADYLFKCGARTTMIGLDVTHQTVLTREK
CCCEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
AHEWDALGTPAGDFLVTMTDYYIDFYVKNQPEIHGCGLHDPLAVAAALDPSLITTFGFNL
HHCCCCCCCCCCCEEEEEEEEEEEEEECCCCCEECCCCCCHHHHHHHCCCHHHEEECCEE
QVDLEGPFRGRTIGDRSRLQDFDKHTQVALGVDVSEFLDRFMARVTAAARG
EEEECCCCCCCCCCCHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA