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The map label for this gene is ptsI [H]

Identifier: 213691099

GI number: 213691099

Start: 211473

End: 213122

Strand: Direct

Name: ptsI [H]

Synonym: Blon_0178

Alternate gene names: 213691099

Gene position: 211473-213122 (Clockwise)

Preceding gene: 213691096

Following gene: 213691100

Centisome position: 7.47

GC content: 66.67

Gene sequence:

>1650_bases
ATGGAAATCAAGGGTGTTGGCATTGGCCGCGGCGTGGCGGTCGGCTCGGTTATTCGTATGGCGGCCCCTCTGCCGGAGCC
TTCCGACGCGCCCCGTGCCGATACCGTGCCGGCACAGAAAGAGATCGACCGCGTCACTAAGTCGCTGGCCGTGGTGAATG
CCGACCTGAGCCGTCGTGCTGAAGAAGCGGCCAACGGCGACGAAGGCGCCAAGCAGGCCGCCCCGATTCTGCAGGCCATC
TCCATGTTCGCGTCCGACCCCTCGCTGGCGGCCAGCATCACCTCGCTGATCAACGGCGGCAAAACCGCAGAGCGCGCCGT
ACTGGAAGGCTTCGCCGCAGTTGAGGATATGTTCCGCGCCATCGGCGGATACCAGGCCGAGCGTGCCGCCGATCTGCACG
ATGTGGGCCAGCGCGTGATCGCTGACCTGATGGGCGTGCCTGCTCCCGGCATTCCGGAAAGCGAGACCCCGTTCGTGCTG
GTGGCCGAGGATCTTTCCCCCGCGGACACCGCCGCAATCGACCTGAACAAGGTGCTGGCCATCGTCACTTCGCAGGGCGG
CCCAACCTCGCACACCGCCATTCTGGCCCGCGCTCGCGGCATCGTGGCCGTGGTCTCCGCCGCCGAAGCCGAGAACCTGG
CCAATGGTGAGCAAGTCATCGTCAACGCCGCCAAGGGCGTGGTGGTTACCAACCCGACCGCTGCTGAGATCGAGGATGCC
GAGGCCGCCAAGGCTCACGCCGCCAAGGCCAAGGAGCTGCGCGGCAAGCCGGGCTCCACCAAGGACGGCCACCACATTCC
GCTGCTCGCCAACGTGGGCAAGCCCGCTGATGCCGACCCGGCCCTGGAGTATGGCGCCGAAGGCGTGGGCCTGTTCCGCT
CCGAGTTCCTGTTTATCGGCAACTCCGAGCCGCCGAGCGTGGAAGAGCAGACCAAGGCCTACGCCGAGCTGCTGAGCCGC
TTCCCCGGCAAGAAGGTCGTGATCCGTATGCTGGACGCCGGCGCAGATAAACCGCTGCCGTTCCTCACCCCGGAAGACGA
GCCGAACCCCGCCCTCGGCCTGCGTGGTCTGCGCACGCTGAAGGCTCACATGAACGTGCTCGAAGGCCAGCTCAAGGCGC
TCGCCGCCGCCGATGCCCAGACCGAGGCCGACCTGTGGGTCATGGCCCCGATGGTCGCTGACGAGCATGAGGCCGATTAC
TTCGTCAAGCTCGGCAAGTCCTTCGGGCTGAAGAAGGTCGGTGTGATGGCCGAGGTGCCGTCCATCGCGCTTATGGCAGA
CAAGGTGGCCGAGGTGGCCGACTTCGTTTCGATCGGCACCAACGATCTGACCCAGTACACGCTGGCCGCCGACCGTACGC
TCGGCTCGGTCGCCAACTACCAGACCGCATGGCACCCGGCGGTGCTGCGCGCCATCAAGCTCATCGCCGACGCCGGCAAC
GCGCACGGCATGCCGGTGGGCGTGTGCGGTGAGGCTGCCGCCGATCCGGATTTGGCCGTGGTGCTTGCAGGCATCGGCGT
CAACTCGCTGTCCATGACCCCGGTGGCTCTGGACGATGTGCGCGCCGAGCTGGCCAGCTGGACGCTAGAAGAAGCGCAGG
AGAAGGCCGCCAAGGCCCTCAACGGCGACTTCTACACTCCGGCTGAGTGA

Upstream 100 bases:

>100_bases
GGAAGAACTTGCCGGCAATGGCTGCGGCTGCATACTCCGAAGTAGGCATCGAGGCCACTGCTCCAAAGAACTACTACCCT
ACGAACAAAGGAGAACCATT

Downstream 100 bases:

>100_bases
CTGTGGCCGAGCAATCGGCTGAATAATTGGGATGATGCCTTGTTTTCTATCTCTTTGAGAATGGGAAGCAAGGCATTTTC
GGTATTCCAGGGCTTCCCTT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MEIKGVGIGRGVAVGSVIRMAAPLPEPSDAPRADTVPAQKEIDRVTKSLAVVNADLSRRAEEAANGDEGAKQAAPILQAI
SMFASDPSLAASITSLINGGKTAERAVLEGFAAVEDMFRAIGGYQAERAADLHDVGQRVIADLMGVPAPGIPESETPFVL
VAEDLSPADTAAIDLNKVLAIVTSQGGPTSHTAILARARGIVAVVSAAEAENLANGEQVIVNAAKGVVVTNPTAAEIEDA
EAAKAHAAKAKELRGKPGSTKDGHHIPLLANVGKPADADPALEYGAEGVGLFRSEFLFIGNSEPPSVEEQTKAYAELLSR
FPGKKVVIRMLDAGADKPLPFLTPEDEPNPALGLRGLRTLKAHMNVLEGQLKALAAADAQTEADLWVMAPMVADEHEADY
FVKLGKSFGLKKVGVMAEVPSIALMADKVAEVADFVSIGTNDLTQYTLAADRTLGSVANYQTAWHPAVLRAIKLIADAGN
AHGMPVGVCGEAAADPDLAVVLAGIGVNSLSMTPVALDDVRAELASWTLEEAQEKAAKALNGDFYTPAE

Sequences:

>Translated_549_residues
MEIKGVGIGRGVAVGSVIRMAAPLPEPSDAPRADTVPAQKEIDRVTKSLAVVNADLSRRAEEAANGDEGAKQAAPILQAI
SMFASDPSLAASITSLINGGKTAERAVLEGFAAVEDMFRAIGGYQAERAADLHDVGQRVIADLMGVPAPGIPESETPFVL
VAEDLSPADTAAIDLNKVLAIVTSQGGPTSHTAILARARGIVAVVSAAEAENLANGEQVIVNAAKGVVVTNPTAAEIEDA
EAAKAHAAKAKELRGKPGSTKDGHHIPLLANVGKPADADPALEYGAEGVGLFRSEFLFIGNSEPPSVEEQTKAYAELLSR
FPGKKVVIRMLDAGADKPLPFLTPEDEPNPALGLRGLRTLKAHMNVLEGQLKALAAADAQTEADLWVMAPMVADEHEADY
FVKLGKSFGLKKVGVMAEVPSIALMADKVAEVADFVSIGTNDLTQYTLAADRTLGSVANYQTAWHPAVLRAIKLIADAGN
AHGMPVGVCGEAAADPDLAVVLAGIGVNSLSMTPVALDDVRAELASWTLEEAQEKAAKALNGDFYTPAE
>Mature_549_residues
MEIKGVGIGRGVAVGSVIRMAAPLPEPSDAPRADTVPAQKEIDRVTKSLAVVNADLSRRAEEAANGDEGAKQAAPILQAI
SMFASDPSLAASITSLINGGKTAERAVLEGFAAVEDMFRAIGGYQAERAADLHDVGQRVIADLMGVPAPGIPESETPFVL
VAEDLSPADTAAIDLNKVLAIVTSQGGPTSHTAILARARGIVAVVSAAEAENLANGEQVIVNAAKGVVVTNPTAAEIEDA
EAAKAHAAKAKELRGKPGSTKDGHHIPLLANVGKPADADPALEYGAEGVGLFRSEFLFIGNSEPPSVEEQTKAYAELLSR
FPGKKVVIRMLDAGADKPLPFLTPEDEPNPALGLRGLRTLKAHMNVLEGQLKALAAADAQTEADLWVMAPMVADEHEADY
FVKLGKSFGLKKVGVMAEVPSIALMADKVAEVADFVSIGTNDLTQYTLAADRTLGSVANYQTAWHPAVLRAIKLIADAGN
AHGMPVGVCGEAAADPDLAVVLAGIGVNSLSMTPVALDDVRAELASWTLEEAQEKAAKALNGDFYTPAE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=434, Percent_Identity=35.4838709677419, Blast_Score=264, Evalue=9e-72,
Organism=Escherichia coli, GI1789193, Length=547, Percent_Identity=31.6270566727605, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI48994992, Length=486, Percent_Identity=30.0411522633745, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788726, Length=570, Percent_Identity=29.2982456140351, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1787994, Length=471, Percent_Identity=29.5116772823779, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 56959; Mature: 56959

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKGVGIGRGVAVGSVIRMAAPLPEPSDAPRADTVPAQKEIDRVTKSLAVVNADLSRRA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EEAANGDEGAKQAAPILQAISMFASDPSLAASITSLINGGKTAERAVLEGFAAVEDMFRA
HHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IGGYQAERAADLHDVGQRVIADLMGVPAPGIPESETPFVLVAEDLSPADTAAIDLNKVLA
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHEEE
IVTSQGGPTSHTAILARARGIVAVVSAAEAENLANGEQVIVNAAKGVVVTNPTAAEIEDA
EEECCCCCCHHHHHHHHHCCCEEEEEHHHHHHCCCCCEEEEEECCCEEEECCCHHHCCHH
EAAKAHAAKAKELRGKPGSTKDGHHIPLLANVGKPADADPALEYGAEGVGLFRSEFLFIG
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCEEHEEEEEEEE
NSEPPSVEEQTKAYAELLSRFPGKKVVIRMLDAGADKPLPFLTPEDEPNPALGLRGLRTL
CCCCCCHHHHHHHHHHHHHHCCCHHEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
KAHMNVLEGQLKALAAADAQTEADLWVMAPMVADEHEADYFVKLGKSFGLKKVGVMAEVP
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHCCCCCCHHHHHHHHHCCCHHHHHHHHCC
SIALMADKVAEVADFVSIGTNDLTQYTLAADRTLGSVANYQTAWHPAVLRAIKLIADAGN
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
AHGMPVGVCGEAAADPDLAVVLAGIGVNSLSMTPVALDDVRAELASWTLEEAQEKAAKAL
CCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NGDFYTPAE
CCCCCCCCC
>Mature Secondary Structure
MEIKGVGIGRGVAVGSVIRMAAPLPEPSDAPRADTVPAQKEIDRVTKSLAVVNADLSRRA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EEAANGDEGAKQAAPILQAISMFASDPSLAASITSLINGGKTAERAVLEGFAAVEDMFRA
HHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IGGYQAERAADLHDVGQRVIADLMGVPAPGIPESETPFVLVAEDLSPADTAAIDLNKVLA
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHEEE
IVTSQGGPTSHTAILARARGIVAVVSAAEAENLANGEQVIVNAAKGVVVTNPTAAEIEDA
EEECCCCCCHHHHHHHHHCCCEEEEEHHHHHHCCCCCEEEEEECCCEEEECCCHHHCCHH
EAAKAHAAKAKELRGKPGSTKDGHHIPLLANVGKPADADPALEYGAEGVGLFRSEFLFIG
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCEEHEEEEEEEE
NSEPPSVEEQTKAYAELLSRFPGKKVVIRMLDAGADKPLPFLTPEDEPNPALGLRGLRTL
CCCCCCHHHHHHHHHHHHHHCCCHHEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
KAHMNVLEGQLKALAAADAQTEADLWVMAPMVADEHEADYFVKLGKSFGLKKVGVMAEVP
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHCCCCCCHHHHHHHHHCCCHHHHHHHHCC
SIALMADKVAEVADFVSIGTNDLTQYTLAADRTLGSVANYQTAWHPAVLRAIKLIADAGN
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
AHGMPVGVCGEAAADPDLAVVLAGIGVNSLSMTPVALDDVRAELASWTLEEAQEKAAKAL
CCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NGDFYTPAE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]