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The map label for this gene is holB [H]

Identifier: 213691096

GI number: 213691096

Start: 209031

End: 210182

Strand: Direct

Name: holB [H]

Synonym: Blon_0175

Alternate gene names: 213691096

Gene position: 209031-210182 (Clockwise)

Preceding gene: 213691095

Following gene: 213691099

Centisome position: 7.38

GC content: 63.19

Gene sequence:

>1152_bases
ATGAGCGTATGGGATTCACTAGTCGGCCAGCAGCCGGTAATCGACATGTTCAGTCGCATCGCGCAGGGCGACCCGGGGCA
GATCACCCAGTCGTGGCTGATTTGCGGGCCTCCCGGCTCCGGCCGTTCCAATATGGCCCGAGCATTCGCGGCGGCGCTGG
AAAGCCCCGACCGCGGCATGAACGATGAGCCCACGCGCGTCACGCAGCAGGTGCTCGCCGGCACCCACCCGGATGTCACG
GTGCTGGCCACCAACAAAGTGACCATCGGCATCGACCAGGTGCGCGAGATCATCACCACCTCCGAGCAAATGCCTGCCAC
GGCACCATGGCGCATCATCATTATCGAGGATGTGGACCGCATGCTGGAGCGCACCACCAATGTGCTGCTGAAGGAGATCG
AGGAGCCGGCCGAACACTGCATCTGGCTGCTATGCGCGCCCAGCGCCCAGGACGTGCTGCCCACCATCCGCTCGCGTACG
CGCATTGTGAATCTCGCCGTGCCCTCCGCATCGGCGGTGGCTGAATTCTTGACCTCCACTACCAATGTTGAGCCGAAAAT
CGCCCAGCGTGCGGCCCGTCTGGCCGAGGGGCACATCGGCATCGCCAAGTTGTACGCCACTGACAAGCAGGTGATGACGG
ATCGCGATGAATTGGTGGTGGGCGTACTGAACCTCGCTCGCGCCTCGGACGCGGTGCTGCTGGCCGGCAACCTCATCGAC
AACGCCAAGGCCCAGGCCGAGGTGGACGCAAGCCGCACCGCGGCCGATCAAGAGGCGGAATTCCGCCGTGTCAACGGTCT
TGAGCCGGGGGAGCGGATTCCGCCGAAACTGCGCGGCGCGTTCAATCAGATCGCTAAGAAGGACGATCTCAAGCGGCTGA
CCACACGTCGCGCGCGCGATGTACTTGACCGCGCGCTCAATTCCATCGCCTCCATTTACCGTGACGTGGCGGTATTGCAG
AACAACGCGGAAGATTCGGTCGGGCTTATCAACCTGGAGAATCGGTCGGCCATCACCGAGCTATCGGTACGACTGAACCG
TGCCGGGGCAGTGGCCCGTCTGGACGAAGTGGCGCACACCCGCAAGCGGCTTGCCGGCAATGGCAACCCGTTGCTGGTGT
TTGAATCGCTGTTCTGCGCGCTGATTCCGTAA

Upstream 100 bases:

>100_bases
CGGACATTCAGACACTGCTGCGGGACAATGTTGCAGATGTGGATACGGTGATGGCGCGATCCGGGGCGTCTACAGGAGCC
GTGACGATGGGCGGTGTCCG

Downstream 100 bases:

>100_bases
GGTTCTTCCCGCCGCCCGATTTATTCGGCGTCCTGCGAGTCCGGAGCAATCAGGCGCGAAGGCAAGTTGACATGTACTGG
GGTGCTGGATTGATCGGCAA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSVWDSLVGQQPVIDMFSRIAQGDPGQITQSWLICGPPGSGRSNMARAFAAALESPDRGMNDEPTRVTQQVLAGTHPDVT
VLATNKVTIGIDQVREIITTSEQMPATAPWRIIIIEDVDRMLERTTNVLLKEIEEPAEHCIWLLCAPSAQDVLPTIRSRT
RIVNLAVPSASAVAEFLTSTTNVEPKIAQRAARLAEGHIGIAKLYATDKQVMTDRDELVVGVLNLARASDAVLLAGNLID
NAKAQAEVDASRTAADQEAEFRRVNGLEPGERIPPKLRGAFNQIAKKDDLKRLTTRRARDVLDRALNSIASIYRDVAVLQ
NNAEDSVGLINLENRSAITELSVRLNRAGAVARLDEVAHTRKRLAGNGNPLLVFESLFCALIP

Sequences:

>Translated_383_residues
MSVWDSLVGQQPVIDMFSRIAQGDPGQITQSWLICGPPGSGRSNMARAFAAALESPDRGMNDEPTRVTQQVLAGTHPDVT
VLATNKVTIGIDQVREIITTSEQMPATAPWRIIIIEDVDRMLERTTNVLLKEIEEPAEHCIWLLCAPSAQDVLPTIRSRT
RIVNLAVPSASAVAEFLTSTTNVEPKIAQRAARLAEGHIGIAKLYATDKQVMTDRDELVVGVLNLARASDAVLLAGNLID
NAKAQAEVDASRTAADQEAEFRRVNGLEPGERIPPKLRGAFNQIAKKDDLKRLTTRRARDVLDRALNSIASIYRDVAVLQ
NNAEDSVGLINLENRSAITELSVRLNRAGAVARLDEVAHTRKRLAGNGNPLLVFESLFCALIP
>Mature_382_residues
SVWDSLVGQQPVIDMFSRIAQGDPGQITQSWLICGPPGSGRSNMARAFAAALESPDRGMNDEPTRVTQQVLAGTHPDVTV
LATNKVTIGIDQVREIITTSEQMPATAPWRIIIIEDVDRMLERTTNVLLKEIEEPAEHCIWLLCAPSAQDVLPTIRSRTR
IVNLAVPSASAVAEFLTSTTNVEPKIAQRAARLAEGHIGIAKLYATDKQVMTDRDELVVGVLNLARASDAVLLAGNLIDN
AKAQAEVDASRTAADQEAEFRRVNGLEPGERIPPKLRGAFNQIAKKDDLKRLTTRRARDVLDRALNSIASIYRDVAVLQN
NAEDSVGLINLENRSAITELSVRLNRAGAVARLDEVAHTRKRLAGNGNPLLVFESLFCALIP

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=233, Percent_Identity=26.1802575107296, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41724; Mature: 41593

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVWDSLVGQQPVIDMFSRIAQGDPGQITQSWLICGPPGSGRSNMARAFAAALESPDRGM
CCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
NDEPTRVTQQVLAGTHPDVTVLATNKVTIGIDQVREIITTSEQMPATAPWRIIIIEDVDR
CCCHHHHHHHHHCCCCCCEEEEECCCEEECHHHHHHHHHHHHCCCCCCCEEEEEEHHHHH
MLERTTNVLLKEIEEPAEHCIWLLCAPSAQDVLPTIRSRTRIVNLAVPSASAVAEFLTST
HHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHC
TNVEPKIAQRAARLAEGHIGIAKLYATDKQVMTDRDELVVGVLNLARASDAVLLAGNLID
CCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCEEEECCHHH
NAKAQAEVDASRTAADQEAEFRRVNGLEPGERIPPKLRGAFNQIAKKDDLKRLTTRRARD
CCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLDRALNSIASIYRDVAVLQNNAEDSVGLINLENRSAITELSVRLNRAGAVARLDEVAHT
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHH
RKRLAGNGNPLLVFESLFCALIP
HHHHCCCCCEEEHHHHHHHHHCC
>Mature Secondary Structure 
SVWDSLVGQQPVIDMFSRIAQGDPGQITQSWLICGPPGSGRSNMARAFAAALESPDRGM
CHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
NDEPTRVTQQVLAGTHPDVTVLATNKVTIGIDQVREIITTSEQMPATAPWRIIIIEDVDR
CCCHHHHHHHHHCCCCCCEEEEECCCEEECHHHHHHHHHHHHCCCCCCCEEEEEEHHHHH
MLERTTNVLLKEIEEPAEHCIWLLCAPSAQDVLPTIRSRTRIVNLAVPSASAVAEFLTST
HHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHC
TNVEPKIAQRAARLAEGHIGIAKLYATDKQVMTDRDELVVGVLNLARASDAVLLAGNLID
CCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCEEEECCHHH
NAKAQAEVDASRTAADQEAEFRRVNGLEPGERIPPKLRGAFNQIAKKDDLKRLTTRRARD
CCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLDRALNSIASIYRDVAVLQNNAEDSVGLINLENRSAITELSVRLNRAGAVARLDEVAHT
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHH
RKRLAGNGNPLLVFESLFCALIP
HHHHCCCCCEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]