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The map label for this gene is rhdA [H]

Identifier: 213159121

GI number: 213159121

Start: 3953512

End: 3954360

Strand: Reverse

Name: rhdA [H]

Synonym: AB57_3831

Alternate gene names: 213159121

Gene position: 3954360-3953512 (Counterclockwise)

Preceding gene: 213159122

Following gene: 213159120

Centisome position: 97.63

GC content: 43.7

Gene sequence:

>849_bases
ATGACACTCCCTGATTTTAAACTCGATTTATTAATAGAAGCAGAAGAGTTAGTGCCTTATTTAGGCAATGAATATATCCG
TATTGTTGATTTGAGCCGTGCATCAGTTTATGAGCAGCTCCATGTTCCGCATGCTATTCACTTACAACCTAAATGGTTAG
TTGCTCAACACGAAGAAGCAACAGGTGTTTTACCGGATGAAGCGGGTTTACAAGCGCTTATTGAAAAATTGAATATTTCA
CCAAATCACCATGTGGTGGTCTATGATGATGAAGGTGGAGCATGGGCAGGGCGTTTAATCTGGAATCTCCATTGCTTAGG
TTTTACCCGTACAAGTTTAATTAATGGCGGAATTCATGCATGGCTTGGTGCAGGTTTACCCACCACAGCTGAGGTTGAAG
AACTTCCTCGAGTTGATAACTTAATTCAGATCGATTTATCACATGCTGCTCAGTTCAGAGTGAACTATGAAGAACTACGC
AAACAAGTTGAGCAAGAAAGTGTGCAACTCTGGGATTGCCGGACGAGTGATGAATATAGTGGCGTCCGTTTAGCAGCGCG
ACGCGGTGGTCACATTCCAAATGCCCTCCATTTTGAATGGAGTACTGCACTTAATCGTCAAAATCATCTAAAATTGCATC
CGCTTGAACGCACGCGGCAGCGTCTTGAACAACTTGGGTTCGATTTAAAACAACCTGTAGTCGTATACTGCCAGTCACAT
CACCGTTCTGGTTTGGCTTATATTCTGGCTAGACTTTTAGGTTGGGAAGCAAAAGCCTATGATGGTGCGTGGAGTGAATG
GGGCAATCGCCTCGATAGTCCTATCATTACTGGAGAATCGCCGTCTTGA

Upstream 100 bases:

>100_bases
ATATCTGATTTTCTGCTAAACACTAGCAGAAAATCAGAGTTTGATAGATATAAGCTTATGCTAAGATGAAATTAAGCTGG
TATAAAAAGTAACCATGACG

Downstream 100 bases:

>100_bases
GTTTTACATCTCGCTTAAAAAAAGAATTATTTATTAAGGCACAAAATCTTGTTCCTCAGCATCAGTTAAGTCGTGTAGTG
GGTAAAGTTGCTGCAAGTGA

Product: hypothetical protein

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTLPDFKLDLLIEAEELVPYLGNEYIRIVDLSRASVYEQLHVPHAIHLQPKWLVAQHEEATGVLPDEAGLQALIEKLNIS
PNHHVVVYDDEGGAWAGRLIWNLHCLGFTRTSLINGGIHAWLGAGLPTTAEVEELPRVDNLIQIDLSHAAQFRVNYEELR
KQVEQESVQLWDCRTSDEYSGVRLAARRGGHIPNALHFEWSTALNRQNHLKLHPLERTRQRLEQLGFDLKQPVVVYCQSH
HRSGLAYILARLLGWEAKAYDGAWSEWGNRLDSPIITGESPS

Sequences:

>Translated_282_residues
MTLPDFKLDLLIEAEELVPYLGNEYIRIVDLSRASVYEQLHVPHAIHLQPKWLVAQHEEATGVLPDEAGLQALIEKLNIS
PNHHVVVYDDEGGAWAGRLIWNLHCLGFTRTSLINGGIHAWLGAGLPTTAEVEELPRVDNLIQIDLSHAAQFRVNYEELR
KQVEQESVQLWDCRTSDEYSGVRLAARRGGHIPNALHFEWSTALNRQNHLKLHPLERTRQRLEQLGFDLKQPVVVYCQSH
HRSGLAYILARLLGWEAKAYDGAWSEWGNRLDSPIITGESPS
>Mature_281_residues
TLPDFKLDLLIEAEELVPYLGNEYIRIVDLSRASVYEQLHVPHAIHLQPKWLVAQHEEATGVLPDEAGLQALIEKLNISP
NHHVVVYDDEGGAWAGRLIWNLHCLGFTRTSLINGGIHAWLGAGLPTTAEVEELPRVDNLIQIDLSHAAQFRVNYEELRK
QVEQESVQLWDCRTSDEYSGVRLAARRGGHIPNALHFEWSTALNRQNHLKLHPLERTRQRLEQLGFDLKQPVVVYCQSHH
RSGLAYILARLLGWEAKAYDGAWSEWGNRLDSPIITGESPS

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=241, Percent_Identity=28.6307053941909, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI61835204, Length=266, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI194473681, Length=266, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI194473668, Length=266, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI87082121, Length=283, Percent_Identity=25.7950530035336, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081967, Length=222, Percent_Identity=23.8738738738739, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17561888, Length=255, Percent_Identity=22.7450980392157, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71997283, Length=249, Percent_Identity=23.2931726907631, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 32000; Mature: 31868

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPDFKLDLLIEAEELVPYLGNEYIRIVDLSRASVYEQLHVPHAIHLQPKWLVAQHEEA
CCCCCCEEEEEEEHHHHHHHHCCCEEEEEECCHHHHHHHHCCCEEEEECCCEEEEECCCC
TGVLPDEAGLQALIEKLNISPNHHVVVYDDEGGAWAGRLIWNLHCLGFTRTSLINGGIHA
CCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEHHHEEEEEECCCHHHHHHCCCHHH
WLGAGLPTTAEVEELPRVDNLIQIDLSHAAQFRVNYEELRKQVEQESVQLWDCRTSDEYS
HHCCCCCCCHHHHHCCCCCCEEEEEECHHHEEEECHHHHHHHHHHHHEEEEECCCCCCCC
GVRLAARRGGHIPNALHFEWSTALNRQNHLKLHPLERTRQRLEQLGFDLKQPVVVYCQSH
CEEEEHHCCCCCCCEEEEEHHHHCCCCCCEEECCHHHHHHHHHHHCCCCCCCCEEEECCC
HRSGLAYILARLLGWEAKAYDGAWSEWGNRLDSPIITGESPS
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEECCCCCC
>Mature Secondary Structure 
TLPDFKLDLLIEAEELVPYLGNEYIRIVDLSRASVYEQLHVPHAIHLQPKWLVAQHEEA
CCCCCEEEEEEEHHHHHHHHCCCEEEEEECCHHHHHHHHCCCEEEEECCCEEEEECCCC
TGVLPDEAGLQALIEKLNISPNHHVVVYDDEGGAWAGRLIWNLHCLGFTRTSLINGGIHA
CCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEHHHEEEEEECCCHHHHHHCCCHHH
WLGAGLPTTAEVEELPRVDNLIQIDLSHAAQFRVNYEELRKQVEQESVQLWDCRTSDEYS
HHCCCCCCCHHHHHCCCCCCEEEEEECHHHEEEECHHHHHHHHHHHHEEEEECCCCCCCC
GVRLAARRGGHIPNALHFEWSTALNRQNHLKLHPLERTRQRLEQLGFDLKQPVVVYCQSH
CEEEEHHCCCCCCCEEEEEHHHHCCCCCCEEECCHHHHHHHHHHHCCCCCCCCEEEECCC
HRSGLAYILARLLGWEAKAYDGAWSEWGNRLDSPIITGESPS
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]