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The map label for this gene is gshB [H]

Identifier: 213159078

GI number: 213159078

Start: 3907722

End: 3908666

Strand: Reverse

Name: gshB [H]

Synonym: AB57_3788

Alternate gene names: 213159078

Gene position: 3908666-3907722 (Counterclockwise)

Preceding gene: 213159081

Following gene: 213159077

Centisome position: 96.5

GC content: 41.59

Gene sequence:

>945_bases
ATGCGTGTACTTGTCGTCATGGATCCGATTGAAACCGTAAATCTGAAAAAGGATTCAACCATGGCAATGTTGTGGGCGGC
GAGCCGTCGTGGACATGAGTTAGGTTATGCATTACAACAAGATTTATATATCGATCAAGGTAAGGCTTACGGTCTCATCT
CTCCGTTAAAAGTTTTCGAAGATTACAACCATTATTATGAGTTAGGCGAAAAGAAAAAAGAATCAATCGCAGCTTATGAT
GTTGTCCTCATGCGTAAAGACCCGCCTTTTGACATGAACTTCGTCTACACAACTTACGTGCTCGAACAAGCTGAGCGTGA
AGGTTCATGGATTATTAATAAGCCTCAATCACTGCGTGACTGCAATGAAAAATTGTTTGCTACGCAATTTCCTGAATTAC
AGGTGCCAACACTTGTAACATCGCAGCAGAGCTTGATTCGCGAATTTATTACCGAGCACGGTGATGTTATTGTTAAGCCG
CTTGATGGTATGGGTGGTATGGGTATTTTCCGTTTATACCAAGACGGTGTAAATATTGGTTCTACCTTAGAAATGCTTAC
AGAGCTAGGTACTCGTCCAATTATGGCTCAGCGTTATATTCCGGAAATTGTAGAAGGGGATAAGCGTATTTTAATGGTAA
ATGGCGAGCCTATTCCTTACTGTCTAGCACGTATTCCGCAAAATGGTGAAGTACGCGGTAACTTGGCAGCTGGGGGCTTA
GGTCAAGCGCGTCCACTCACAGAAAATGACAAACTCATTGCTGCTAAAGTTGGTCCTTTCTTACGTGAAAAAGGTTTGGT
ATTTGTAGGTCTTGATGTAATTGGGAATTATGTCACTGAAATTAATGTGACAAGTCCAACCTGTATTCGTGAAATTGATG
CGCAATTTGGTACTTCAATTGCCGATGATCTATTTGATGTATTAGAAGCTGGTCGTCCAGCATAA

Upstream 100 bases:

>100_bases
GATAAAGAAATAACCGATAAGGTTTTACTAAAATTAAGAAAAACGAGTGAAAAATCTCAGCTATACTCATGCTAATTTAA
ATTTTAAGAAGAGCTTGAGC

Downstream 100 bases:

>100_bases
GGGTCTAGTTAGGGTGGTTTTCTAAGCTTATTATTGGAAATCACCCGAAAATATATGAATTAATAAAATCACAATACCAA
ATACACCATAGCTTTGTAGG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRVLVVMDPIETVNLKKDSTMAMLWAASRRGHELGYALQQDLYIDQGKAYGLISPLKVFEDYNHYYELGEKKKESIAAYD
VVLMRKDPPFDMNFVYTTYVLEQAEREGSWIINKPQSLRDCNEKLFATQFPELQVPTLVTSQQSLIREFITEHGDVIVKP
LDGMGGMGIFRLYQDGVNIGSTLEMLTELGTRPIMAQRYIPEIVEGDKRILMVNGEPIPYCLARIPQNGEVRGNLAAGGL
GQARPLTENDKLIAAKVGPFLREKGLVFVGLDVIGNYVTEINVTSPTCIREIDAQFGTSIADDLFDVLEAGRPA

Sequences:

>Translated_314_residues
MRVLVVMDPIETVNLKKDSTMAMLWAASRRGHELGYALQQDLYIDQGKAYGLISPLKVFEDYNHYYELGEKKKESIAAYD
VVLMRKDPPFDMNFVYTTYVLEQAEREGSWIINKPQSLRDCNEKLFATQFPELQVPTLVTSQQSLIREFITEHGDVIVKP
LDGMGGMGIFRLYQDGVNIGSTLEMLTELGTRPIMAQRYIPEIVEGDKRILMVNGEPIPYCLARIPQNGEVRGNLAAGGL
GQARPLTENDKLIAAKVGPFLREKGLVFVGLDVIGNYVTEINVTSPTCIREIDAQFGTSIADDLFDVLEAGRPA
>Mature_314_residues
MRVLVVMDPIETVNLKKDSTMAMLWAASRRGHELGYALQQDLYIDQGKAYGLISPLKVFEDYNHYYELGEKKKESIAAYD
VVLMRKDPPFDMNFVYTTYVLEQAEREGSWIINKPQSLRDCNEKLFATQFPELQVPTLVTSQQSLIREFITEHGDVIVKP
LDGMGGMGIFRLYQDGVNIGSTLEMLTELGTRPIMAQRYIPEIVEGDKRILMVNGEPIPYCLARIPQNGEVRGNLAAGGL
GQARPLTENDKLIAAKVGPFLREKGLVFVGLDVIGNYVTEINVTSPTCIREIDAQFGTSIADDLFDVLEAGRPA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=310, Percent_Identity=55.8064516129032, Blast_Score=360, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35113; Mature: 35113

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVVMDPIETVNLKKDSTMAMLWAASRRGHELGYALQQDLYIDQGKAYGLISPLKVFE
CEEEEEECCCCEECCCCCCCEEEEEECCCCCCHHHHHHHHCEEEECCCCCCCHHHHHHHH
DYNHYYELGEKKKESIAAYDVVLMRKDPPFDMNFVYTTYVLEQAEREGSWIINKPQSLRD
HHHHHHHHCCHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHH
CNEKLFATQFPELQVPTLVTSQQSLIREFITEHGDVIVKPLDGMGGMGIFRLYQDGVNIG
HHHHHHHCCCCCCCCCEEECCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCC
STLEMLTELGTRPIMAQRYIPEIVEGDKRILMVNGEPIPYCLARIPQNGEVRGNLAAGGL
HHHHHHHHHCCCCHHHHHHCHHHHCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEEECCC
GQARPLTENDKLIAAKVGPFLREKGLVFVGLDVIGNYVTEINVTSPTCIREIDAQFGTSI
CCCCCCCCCCCEEEEHHCHHHHHCCEEEEEHHHHCCCCEEEECCCCHHHHHHHHHHCCHH
ADDLFDVLEAGRPA
HHHHHHHHHCCCCC
>Mature Secondary Structure
MRVLVVMDPIETVNLKKDSTMAMLWAASRRGHELGYALQQDLYIDQGKAYGLISPLKVFE
CEEEEEECCCCEECCCCCCCEEEEEECCCCCCHHHHHHHHCEEEECCCCCCCHHHHHHHH
DYNHYYELGEKKKESIAAYDVVLMRKDPPFDMNFVYTTYVLEQAEREGSWIINKPQSLRD
HHHHHHHHCCHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHH
CNEKLFATQFPELQVPTLVTSQQSLIREFITEHGDVIVKPLDGMGGMGIFRLYQDGVNIG
HHHHHHHCCCCCCCCCEEECCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCC
STLEMLTELGTRPIMAQRYIPEIVEGDKRILMVNGEPIPYCLARIPQNGEVRGNLAAGGL
HHHHHHHHHCCCCHHHHHHCHHHHCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEEECCC
GQARPLTENDKLIAAKVGPFLREKGLVFVGLDVIGNYVTEINVTSPTCIREIDAQFGTSI
CCCCCCCCCCCEEEEHHCHHHHHCCEEEEEHHHHCCCCEEEECCCCHHHHHHHHHHCCHH
ADDLFDVLEAGRPA
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]