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The map label for this gene is pyrE [H]

Identifier: 213159081

GI number: 213159081

Start: 3911509

End: 3912159

Strand: Reverse

Name: pyrE [H]

Synonym: AB57_3791

Alternate gene names: 213159081

Gene position: 3912159-3911509 (Counterclockwise)

Preceding gene: 213159084

Following gene: 213159078

Centisome position: 96.58

GC content: 41.32

Gene sequence:

>651_bases
ATGACAACGCCTGTGTCATTTCACCCGCAAGCATTTATCGAACTCGCATTATCACGCGGTGTGCTTAAATTTGGTGAGTT
TACTTTAAAATCTGGACGCGTGAGTCCTTATTTTTTTAATGCAGGCCTACTCAACGATGGTGAAGCTTTATCTCTTTTAG
CACAAGGCTATGCCGATAAATTAACACAATGTGAAAATGTTGATGTGATTTTTGGACCAGCTTACAAAGGTATTCCATTT
GTAGCTGCGACTGCTGTTGCTTTATCGCAAACACATAACAAGAGCGTGCCATGGGGCTTTAACCGTAAAGAAGCAAAAGA
TCATGGTGAAGGCGGTATTTTAGTCGGTGCTGCGGTTGAAGGTAAAAAAGTCTGGATCATTGATGACGTGATTACCGCAG
GCACAGCGATTCGCGAAGTGGTAACGATTTTGAAAAATGCCGGTGCAACTATTGCGGGTGTATTGGTTGCGCTAGATCGT
CAAGAACGTGGTCAAGGTGAACTTTCTGCAATTCAAGAAGTTCAAAAAGAATTAGAAATACCTGTACATGCTCTTATTAC
CATGAAGGACTTAATGGATTATTTAGAAGCAAAAGGCGAAAAAGAAGCGCTAGCAAACATGCAAGCTTATCGCGAGAAAT
ATGGTATCTAA

Upstream 100 bases:

>100_bases
ACGTTAATGGAAACGACTCGAAGAATTTTTACATCACTTGGATACTGATCCTTTGGTATCATGCGAAAATTTAACCTCAA
TTGTGAATTTGGAGCACCTC

Downstream 100 bases:

>100_bases
AAAGAAAAAGGCATACCTCTAAAATAGGGGTATGCCTTTTTAATATTTACTTAAAACTGATAATTCAAACGCAATAACAC
ATTACGTGGTGTGCCTGGCA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTTPVSFHPQAFIELALSRGVLKFGEFTLKSGRVSPYFFNAGLLNDGEALSLLAQGYADKLTQCENVDVIFGPAYKGIPF
VAATAVALSQTHNKSVPWGFNRKEAKDHGEGGILVGAAVEGKKVWIIDDVITAGTAIREVVTILKNAGATIAGVLVALDR
QERGQGELSAIQEVQKELEIPVHALITMKDLMDYLEAKGEKEALANMQAYREKYGI

Sequences:

>Translated_216_residues
MTTPVSFHPQAFIELALSRGVLKFGEFTLKSGRVSPYFFNAGLLNDGEALSLLAQGYADKLTQCENVDVIFGPAYKGIPF
VAATAVALSQTHNKSVPWGFNRKEAKDHGEGGILVGAAVEGKKVWIIDDVITAGTAIREVVTILKNAGATIAGVLVALDR
QERGQGELSAIQEVQKELEIPVHALITMKDLMDYLEAKGEKEALANMQAYREKYGI
>Mature_215_residues
TTPVSFHPQAFIELALSRGVLKFGEFTLKSGRVSPYFFNAGLLNDGEALSLLAQGYADKLTQCENVDVIFGPAYKGIPFV
AATAVALSQTHNKSVPWGFNRKEAKDHGEGGILVGAAVEGKKVWIIDDVITAGTAIREVVTILKNAGATIAGVLVALDRQ
ERGQGELSAIQEVQKELEIPVHALITMKDLMDYLEAKGEKEALANMQAYREKYGI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=184, Percent_Identity=29.8913043478261, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790073, Length=211, Percent_Identity=59.7156398104265, Blast_Score=249, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI17508631, Length=191, Percent_Identity=27.2251308900524, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=183, Percent_Identity=27.8688524590164, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323530, Length=216, Percent_Identity=47.2222222222222, Blast_Score=195, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6323927, Length=223, Percent_Identity=44.3946188340807, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23349; Mature: 23218

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPVSFHPQAFIELALSRGVLKFGEFTLKSGRVSPYFFNAGLLNDGEALSLLAQGYADK
CCCCCCCCHHHHHHHHHHHCHHHHCCEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
LTQCENVDVIFGPAYKGIPFVAATAVALSQTHNKSVPWGFNRKEAKDHGEGGILVGAAVE
HHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCEEEEEEEC
GKKVWIIDDVITAGTAIREVVTILKNAGATIAGVLVALDRQERGQGELSAIQEVQKELEI
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
PVHALITMKDLMDYLEAKGEKEALANMQAYREKYGI
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTPVSFHPQAFIELALSRGVLKFGEFTLKSGRVSPYFFNAGLLNDGEALSLLAQGYADK
CCCCCCCHHHHHHHHHHHCHHHHCCEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
LTQCENVDVIFGPAYKGIPFVAATAVALSQTHNKSVPWGFNRKEAKDHGEGGILVGAAVE
HHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCEEEEEEEC
GKKVWIIDDVITAGTAIREVVTILKNAGATIAGVLVALDRQERGQGELSAIQEVQKELEI
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
PVHALITMKDLMDYLEAKGEKEALANMQAYREKYGI
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA