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The map label for this gene is phbC [H]

Identifier: 213158627

GI number: 213158627

Start: 2673287

End: 2674435

Strand: Direct

Name: phbC [H]

Synonym: AB57_2581

Alternate gene names: 213158627

Gene position: 2673287-2674435 (Clockwise)

Preceding gene: 213158625

Following gene: 213158628

Centisome position: 66.0

GC content: 40.38

Gene sequence:

>1149_bases
ATGAGTTTATTGCTTAAAGTTCGACAGAATGTTCCACAGGTACAGTCGATTAAAACATCTCTTAAAAAGCGTGCTGGTCA
GAAAATACCTCACATGCTCTCCAATGCATCAGATCTGTTTTTTAGAGGGTCGGAAATTGCCCTGTCAGGAAAAACACCTT
TTGATGTAATTTATAAGCATGAAATTATAAGCCTTCGCCATTATAGAAATGAGACAGAAGCATCAGCCAAACACCGTGTG
CCGTTAGTGATTGTTCCACCTTTAGCTGTGAACATGCTCATTTACGATTTGTTTCCTACCCGCAGCCTGATACGTTATTT
TTTAGATCAAGGTTTCGAGGTTTATTTAATAGACTGGGGTGTACCTACTCGCAATCAAGCCAAATACAATTTTGGCACCT
ATGTAAAAGTCTTTATGCCTGAAATGTTAAAGCAAGTCCGTATACATAGTGGACAACAACAGCTTTCATTACATGGCTGG
AGTTTAGGTGGCGCCCTTTCACTTTGCTATACCGCATTATTTAAAGATAAAGATATTAAGAATCTGATGATTCTTGCTTC
CCCGATCGACACACATAAAGCAGGTTATATGGGTAAGCTCTATGGAAGTCTGACTAAACCTGCACAGTGGGTCCGTAAGC
ATACGCCTTTTCGTATTCGCCAACATGTACCAAGTGAAGTGTTTCATATTCATGGATGGCAAAACACACTCGGTTTTAAA
TTAACGGACCCCATTGGCAATTTAAAAACCTATTGGCAACTTCTTAAAAATCTGGATAACCGAGAATTTATTGTTGACCA
CGCTACCTCTAGTTCATTTATCGACAATATGCTGGCCTATCCGGGTGGCGTTATGCGCGACATTATTTTACGTTTCTGGA
TTGATAATGAACTCTCAACAGGTGTAATCAAATTTGGCGAACTTACAGCTTATTTAAAAGATATTGATTGTTCAGTGTTG
GCTGTAGGTGGAGATACAGACATTATTGTGACCGCCGATGCTGTTAAGCCTCTTATGGATTTAATTAGTAGCCAAGATAA
AACGTTCAAAACTGTACCCGGAGGCCATATGGGCGTAGTGTCCGGTAGCCAAGCTCCGACCTCTGTTTGGCCTGAAATGA
GTAATTGGCTGGCTACACGTTCAGATTAA

Upstream 100 bases:

>100_bases
GTCTTTTAGGTCACTGAACTCATCTTTTATTCCCTTTAATATCCGCAAAAGACCAGCTCGTTATTTGCTCTGGGCAACAT
CTTTATAGGTTTTATATTCA

Downstream 100 bases:

>100_bases
TCAATTTAAACGTTAATGAAAAACCAGCCTGTTAAACCTTAGGCTGGTTTTAATGCACACTAAATTTTTAAAGTTTTTGC
ACAAATCTTCTAGAACTAAT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSLLLKVRQNVPQVQSIKTSLKKRAGQKIPHMLSNASDLFFRGSEIALSGKTPFDVIYKHEIISLRHYRNETEASAKHRV
PLVIVPPLAVNMLIYDLFPTRSLIRYFLDQGFEVYLIDWGVPTRNQAKYNFGTYVKVFMPEMLKQVRIHSGQQQLSLHGW
SLGGALSLCYTALFKDKDIKNLMILASPIDTHKAGYMGKLYGSLTKPAQWVRKHTPFRIRQHVPSEVFHIHGWQNTLGFK
LTDPIGNLKTYWQLLKNLDNREFIVDHATSSSFIDNMLAYPGGVMRDIILRFWIDNELSTGVIKFGELTAYLKDIDCSVL
AVGGDTDIIVTADAVKPLMDLISSQDKTFKTVPGGHMGVVSGSQAPTSVWPEMSNWLATRSD

Sequences:

>Translated_382_residues
MSLLLKVRQNVPQVQSIKTSLKKRAGQKIPHMLSNASDLFFRGSEIALSGKTPFDVIYKHEIISLRHYRNETEASAKHRV
PLVIVPPLAVNMLIYDLFPTRSLIRYFLDQGFEVYLIDWGVPTRNQAKYNFGTYVKVFMPEMLKQVRIHSGQQQLSLHGW
SLGGALSLCYTALFKDKDIKNLMILASPIDTHKAGYMGKLYGSLTKPAQWVRKHTPFRIRQHVPSEVFHIHGWQNTLGFK
LTDPIGNLKTYWQLLKNLDNREFIVDHATSSSFIDNMLAYPGGVMRDIILRFWIDNELSTGVIKFGELTAYLKDIDCSVL
AVGGDTDIIVTADAVKPLMDLISSQDKTFKTVPGGHMGVVSGSQAPTSVWPEMSNWLATRSD
>Mature_381_residues
SLLLKVRQNVPQVQSIKTSLKKRAGQKIPHMLSNASDLFFRGSEIALSGKTPFDVIYKHEIISLRHYRNETEASAKHRVP
LVIVPPLAVNMLIYDLFPTRSLIRYFLDQGFEVYLIDWGVPTRNQAKYNFGTYVKVFMPEMLKQVRIHSGQQQLSLHGWS
LGGALSLCYTALFKDKDIKNLMILASPIDTHKAGYMGKLYGSLTKPAQWVRKHTPFRIRQHVPSEVFHIHGWQNTLGFKL
TDPIGNLKTYWQLLKNLDNREFIVDHATSSSFIDNMLAYPGGVMRDIILRFWIDNELSTGVIKFGELTAYLKDIDCSVLA
VGGDTDIIVTADAVKPLMDLISSQDKTFKTVPGGHMGVVSGSQAPTSVWPEMSNWLATRSD

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010125 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 43154; Mature: 43022

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLLKVRQNVPQVQSIKTSLKKRAGQKIPHMLSNASDLFFRGSEIALSGKTPFDVIYKH
CCEEHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCHHEEECCEEEECCCCCHHHHHHH
EIISLRHYRNETEASAKHRVPLVIVPPLAVNMLIYDLFPTRSLIRYFLDQGFEVYLIDWG
HHHHHHHHCCCHHHHHHHCCCEEEECHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECC
VPTRNQAKYNFGTYVKVFMPEMLKQVRIHSGQQQLSLHGWSLGGALSLCYTALFKDKDIK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCC
NLMILASPIDTHKAGYMGKLYGSLTKPAQWVRKHTPFRIRQHVPSEVFHIHGWQNTLGFK
EEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHCCCHHHHHCCCCHHEEEECCCCCCCEE
LTDPIGNLKTYWQLLKNLDNREFIVDHATSSSFIDNMLAYPGGVMRDIILRFWIDNELST
EECCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
GVIKFGELTAYLKDIDCSVLAVGGDTDIIVTADAVKPLMDLISSQDKTFKTVPGGHMGVV
CEEEHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCEEEECCCCCEEEE
SGSQAPTSVWPEMSNWLATRSD
CCCCCCCHHCHHHHHHHCCCCC
>Mature Secondary Structure 
SLLLKVRQNVPQVQSIKTSLKKRAGQKIPHMLSNASDLFFRGSEIALSGKTPFDVIYKH
CEEHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCHHEEECCEEEECCCCCHHHHHHH
EIISLRHYRNETEASAKHRVPLVIVPPLAVNMLIYDLFPTRSLIRYFLDQGFEVYLIDWG
HHHHHHHHCCCHHHHHHHCCCEEEECHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECC
VPTRNQAKYNFGTYVKVFMPEMLKQVRIHSGQQQLSLHGWSLGGALSLCYTALFKDKDIK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCC
NLMILASPIDTHKAGYMGKLYGSLTKPAQWVRKHTPFRIRQHVPSEVFHIHGWQNTLGFK
EEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHCCCHHHHHCCCCHHEEEECCCCCCCEE
LTDPIGNLKTYWQLLKNLDNREFIVDHATSSSFIDNMLAYPGGVMRDIILRFWIDNELST
EECCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
GVIKFGELTAYLKDIDCSVLAVGGDTDIIVTADAVKPLMDLISSQDKTFKTVPGGHMGVV
CEEEHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCEEEECCCCCEEEE
SGSQAPTSVWPEMSNWLATRSD
CCCCCCCHHCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1396692 [H]