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The map label for this gene is purN [H]

Identifier: 213158292

GI number: 213158292

Start: 3108526

End: 3109155

Strand: Direct

Name: purN [H]

Synonym: AB57_3015

Alternate gene names: 213158292

Gene position: 3108526-3109155 (Clockwise)

Preceding gene: 213158291

Following gene: 213158295

Centisome position: 76.74

GC content: 40.63

Gene sequence:

>630_bases
ATGATGAAAATTGCTGTTCTAGTCTCTGGGAACGGCAGCAACTTACAAGCCTTGATAGATGCAAATCTATCAGGGCAAAT
TGTAGGTGTACTGTCTAATAAAGCCGATGCTTATGCCCTAGAGCGTGCTCAAAATGCCAATATCGCGACAGCCGTAATCT
CTCACAAAGACTTTCCTAGTCGTGAAGATTTTGATGAAGCTATGCATCAGCAACTTATCGCATGGCAAGCTGACGTGGTT
ATTTTAGCTGGCTTTATGCGTATTTTAACTGCTGACTTTGTCAACAAATGGCAAGGTAAAATGCTTAATATCCATCCTTC
ATTATTACCTGCCTATAAAGGCATTAATACACATCAACGTGTATTAAATACGGGGGATCGATTACATGGCTGTACCGTTC
ACTTTGTGACTTCTGAACTTGATGCTGGTCAAGCAATTGCTCAATCTGCCATTGAAGTAAAAGAACATGACAATGTTGCA
TCCTTGGCCGAACGTGTACATAAACTTGAGCATTTTATTTATCCTCAAGTTGCAGAGTGGTTATGTAATGGCCAGCTCAC
ATGGAAAAATGGACAAGCCTATTTCCGCAATCAAATTCTTGAACACCCTATCCGTTTTGCATCTTGGTAA

Upstream 100 bases:

>100_bases
TGCTTAATGCTCAAGGTGAAAAAGCTTGGAAAATCGGACATATCCAAGAAAATGCTGAATCAGTGGAAGGTGCAGACGAA
AAAATTCGTGTGATCTTTGA

Downstream 100 bases:

>100_bases
AAGTTTGCTTAACGTCAATTAAAAAAGGACATGCATGCATGTCCTTTTTTTTGCGAAAGCTTTATTAGCGTGAATTACGG
TGAATAAGTTCCTTAATATA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MMKIAVLVSGNGSNLQALIDANLSGQIVGVLSNKADAYALERAQNANIATAVISHKDFPSREDFDEAMHQQLIAWQADVV
ILAGFMRILTADFVNKWQGKMLNIHPSLLPAYKGINTHQRVLNTGDRLHGCTVHFVTSELDAGQAIAQSAIEVKEHDNVA
SLAERVHKLEHFIYPQVAEWLCNGQLTWKNGQAYFRNQILEHPIRFASW

Sequences:

>Translated_209_residues
MMKIAVLVSGNGSNLQALIDANLSGQIVGVLSNKADAYALERAQNANIATAVISHKDFPSREDFDEAMHQQLIAWQADVV
ILAGFMRILTADFVNKWQGKMLNIHPSLLPAYKGINTHQRVLNTGDRLHGCTVHFVTSELDAGQAIAQSAIEVKEHDNVA
SLAERVHKLEHFIYPQVAEWLCNGQLTWKNGQAYFRNQILEHPIRFASW
>Mature_209_residues
MMKIAVLVSGNGSNLQALIDANLSGQIVGVLSNKADAYALERAQNANIATAVISHKDFPSREDFDEAMHQQLIAWQADVV
ILAGFMRILTADFVNKWQGKMLNIHPSLLPAYKGINTHQRVLNTGDRLHGCTVHFVTSELDAGQAIAQSAIEVKEHDNVA
SLAERVHKLEHFIYPQVAEWLCNGQLTWKNGQAYFRNQILEHPIRFASW

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=195, Percent_Identity=51.2820512820513, Blast_Score=194, Evalue=5e-50,
Organism=Homo sapiens, GI209869995, Length=195, Percent_Identity=51.2820512820513, Blast_Score=194, Evalue=5e-50,
Organism=Homo sapiens, GI209869993, Length=195, Percent_Identity=51.2820512820513, Blast_Score=194, Evalue=5e-50,
Organism=Escherichia coli, GI1788846, Length=203, Percent_Identity=47.2906403940887, Blast_Score=189, Evalue=8e-50,
Organism=Escherichia coli, GI1787483, Length=155, Percent_Identity=32.9032258064516, Blast_Score=85, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=178, Percent_Identity=36.5168539325843, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=200, Percent_Identity=33, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=46.2765957446808, Blast_Score=183, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23310; Mature: 23310

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIAVLVSGNGSNLQALIDANLSGQIVGVLSNKADAYALERAQNANIATAVISHKDFPS
CEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCC
REDFDEAMHQQLIAWQADVVILAGFMRILTADFVNKWQGKMLNIHPSLLPAYKGINTHQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCCHHHH
VLNTGDRLHGCTVHFVTSELDAGQAIAQSAIEVKEHDNVASLAERVHKLEHFIYPQVAEW
HHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LCNGQLTWKNGQAYFRNQILEHPIRFASW
HHCCEEEECCCHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MMKIAVLVSGNGSNLQALIDANLSGQIVGVLSNKADAYALERAQNANIATAVISHKDFPS
CEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCC
REDFDEAMHQQLIAWQADVVILAGFMRILTADFVNKWQGKMLNIHPSLLPAYKGINTHQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCCHHHH
VLNTGDRLHGCTVHFVTSELDAGQAIAQSAIEVKEHDNVASLAERVHKLEHFIYPQVAEW
HHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LCNGQLTWKNGQAYFRNQILEHPIRFASW
HHCCEEEECCCHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]