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The map label for this gene is dacD [H]

Identifier: 213158204

GI number: 213158204

Start: 3018756

End: 3020075

Strand: Direct

Name: dacD [H]

Synonym: AB57_2923

Alternate gene names: 213158204

Gene position: 3018756-3020075 (Clockwise)

Preceding gene: 213158201

Following gene: 213158205

Centisome position: 74.53

GC content: 38.48

Gene sequence:

>1320_bases
GTGAAATTCTTCCTATCTCTTTTTACGCTGTTTAGTATTTTCTGTACTACTCTTACCAATGCTGCTCTACTTAATATTGC
TCCTGAAAGTGTTGAAGCCGCTGCATGGACAATTGTAGATACCCAATCTGGTCAAATTATTGCTGAGCATAATAGTCATG
TACAACGTGCGCCAGCCTCTTTAACCAAAATGATGGTGGCCTACATTGCATTAAAAGAGATTAAGGCTGGAAAGTTAAAA
CTGAACGAAGTCATTACTGCAACACCAGTCGTAAGCGTGGTGCAGTGGGATGAATCACAAATGTACCTCAAAGCGGGCGA
GCAAATTTCAGTTGATCAGCTCTTGGCTGGTTTAATTGTTATGTCTGCAAATGATGCAGCTGTGACTTTGGCTGAAAAAA
TTTCTGGTGATGTGCCTCATTTCGTACAACGTATGAATCAAGAAGCACAAGCACTGGGTATGAAAGACACGCACTTTAGT
AACCCTGCTGGTATTACAATGCCGGATCATTACACGACAGCTCATGATTTGAGCTTATTAAGTCAGGCAGTGATTCACCA
GACCCCTGAATATTTACATTATTCGAAAATGCCGAGCTTTAGCTACAATCAACGTTTTCACCACGCGACGAACCTTGCTT
TAAAATATGACCCATCTGTAGATGGCTTAAAAACTGGCTATACCAAAGCAGCAGGTTATAACTTGGCTCTAACAGCTTCA
CGCCCTTCATTTTCACCGAACTTACCGCAAAGACGTTTACTGGTAATTGTCTTAGGTACACCAAGTGCTGTGAAACGTGC
TGAAATTGCAGATAAATTAATGAATCTTGCTTATGCTTATACACGTGATGAAGTGGTTATTCCTGAGCAAAAATTAATTG
CTGAATTGCCTGTTATTAAATCAACTTTAAAAATGTTTAAAGTAGAAACTAAGCAGCCAACCATTGTTACGACTTCTTTA
TATGCTGAGCCAACACCAATTGATTTAAATACGTTTGATTATGCTACACAGCGTATTCAAGTTCTTGATAGCAATCAACA
ACCTAAAGTTATTGCTCCACTTGAAACAACTCAAACACGTGTAAATATTCAGTTAAATGAGCAAAAGTTAACAGCACCGT
TAATGAAAGCGATGAATTTGGCAACAGTTTCAATTTATCAAAACAACCAGCTCATTCGCAGTCTTCAAATTGAAAATGAT
GTACACATTGAAGAAGCAAATATTTTCCAAAGAATTATGATGTGGTTCTCAAGCCTATTCTCAATCTTTTCATCAAGTGA
GCACAGTGCAGCAAAACTCTACCCTATAGATTCGCACTAA

Upstream 100 bases:

>100_bases
TATTTTTTGGCAACAATTCACACTAGCCTTCTACAAAAAAAAGGCATAGCATGCTGGGCATCTGTGTTCCGTATTTGCCA
GTAAACGTCAAGGTCACCTA

Downstream 100 bases:

>100_bases
AAACATCAAATTGTGCACAGATGCACAATAAATTTTATAAAGAAAAGATGGCATAATTCAGCCATCTTTTTTTTATAGAA
AAATAATTAGGTGATAAAAA

Product: D-ala-D-ala-carboxypeptidase penicillin-binding protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6b; PBP-6b [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKFFLSLFTLFSIFCTTLTNAALLNIAPESVEAAAWTIVDTQSGQIIAEHNSHVQRAPASLTKMMVAYIALKEIKAGKLK
LNEVITATPVVSVVQWDESQMYLKAGEQISVDQLLAGLIVMSANDAAVTLAEKISGDVPHFVQRMNQEAQALGMKDTHFS
NPAGITMPDHYTTAHDLSLLSQAVIHQTPEYLHYSKMPSFSYNQRFHHATNLALKYDPSVDGLKTGYTKAAGYNLALTAS
RPSFSPNLPQRRLLVIVLGTPSAVKRAEIADKLMNLAYAYTRDEVVIPEQKLIAELPVIKSTLKMFKVETKQPTIVTTSL
YAEPTPIDLNTFDYATQRIQVLDSNQQPKVIAPLETTQTRVNIQLNEQKLTAPLMKAMNLATVSIYQNNQLIRSLQIEND
VHIEEANIFQRIMMWFSSLFSIFSSSEHSAAKLYPIDSH

Sequences:

>Translated_439_residues
MKFFLSLFTLFSIFCTTLTNAALLNIAPESVEAAAWTIVDTQSGQIIAEHNSHVQRAPASLTKMMVAYIALKEIKAGKLK
LNEVITATPVVSVVQWDESQMYLKAGEQISVDQLLAGLIVMSANDAAVTLAEKISGDVPHFVQRMNQEAQALGMKDTHFS
NPAGITMPDHYTTAHDLSLLSQAVIHQTPEYLHYSKMPSFSYNQRFHHATNLALKYDPSVDGLKTGYTKAAGYNLALTAS
RPSFSPNLPQRRLLVIVLGTPSAVKRAEIADKLMNLAYAYTRDEVVIPEQKLIAELPVIKSTLKMFKVETKQPTIVTTSL
YAEPTPIDLNTFDYATQRIQVLDSNQQPKVIAPLETTQTRVNIQLNEQKLTAPLMKAMNLATVSIYQNNQLIRSLQIEND
VHIEEANIFQRIMMWFSSLFSIFSSSEHSAAKLYPIDSH
>Mature_439_residues
MKFFLSLFTLFSIFCTTLTNAALLNIAPESVEAAAWTIVDTQSGQIIAEHNSHVQRAPASLTKMMVAYIALKEIKAGKLK
LNEVITATPVVSVVQWDESQMYLKAGEQISVDQLLAGLIVMSANDAAVTLAEKISGDVPHFVQRMNQEAQALGMKDTHFS
NPAGITMPDHYTTAHDLSLLSQAVIHQTPEYLHYSKMPSFSYNQRFHHATNLALKYDPSVDGLKTGYTKAAGYNLALTAS
RPSFSPNLPQRRLLVIVLGTPSAVKRAEIADKLMNLAYAYTRDEVVIPEQKLIAELPVIKSTLKMFKVETKQPTIVTTSL
YAEPTPIDLNTFDYATQRIQVLDSNQQPKVIAPLETTQTRVNIQLNEQKLTAPLMKAMNLATVSIYQNNQLIRSLQIEND
VHIEEANIFQRIMMWFSSLFSIFSSSEHSAAKLYPIDSH

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=421, Percent_Identity=30.166270783848, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1787062, Length=419, Percent_Identity=30.0715990453461, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1786851, Length=430, Percent_Identity=28.8372093023256, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87082054, Length=217, Percent_Identity=30.4147465437788, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48873; Mature: 48873

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFLSLFTLFSIFCTTLTNAALLNIAPESVEAAAWTIVDTQSGQIIAEHNSHVQRAPAS
CHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCEEECCCCHHHHHHHH
LTKMMVAYIALKEIKAGKLKLNEVITATPVVSVVQWDESQMYLKAGEQISVDQLLAGLIV
HHHHHHHHHHHHHHCCCCEEHHHEEECCCEEEEEEECCCCEEECCCCCCCHHHHHHHHEE
MSANDAAVTLAEKISGDVPHFVQRMNQEAQALGMKDTHFSNPAGITMPDHYTTAHDLSLL
EECCCHHEEEHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SQAVIHQTPEYLHYSKMPSFSYNQRFHHATNLALKYDPSVDGLKTGYTKAAGYNLALTAS
HHHHHHCCHHHHHHHCCCCCCCCCCHHHHHCEEEEECCCCCCCCCCCCCCCCCEEEEEEC
RPSFSPNLPQRRLLVIVLGTPSAVKRAEIADKLMNLAYAYTRDEVVIPEQKLIAELPVIK
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHH
STLKMFKVETKQPTIVTTSLYAEPTPIDLNTFDYATQRIQVLDSNQQPKVIAPLETTQTR
HHHHHHEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHEECCCCCCCEEECCCCCCEE
VNIQLNEQKLTAPLMKAMNLATVSIYQNNQLIRSLQIENDVHIEEANIFQRIMMWFSSLF
EEEEECHHHHHHHHHHHHCCEEEEEEECCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHH
SIFSSSEHSAAKLYPIDSH
HHHCCCCCCCEEEEECCCC
>Mature Secondary Structure
MKFFLSLFTLFSIFCTTLTNAALLNIAPESVEAAAWTIVDTQSGQIIAEHNSHVQRAPAS
CHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCEEECCCCHHHHHHHH
LTKMMVAYIALKEIKAGKLKLNEVITATPVVSVVQWDESQMYLKAGEQISVDQLLAGLIV
HHHHHHHHHHHHHHCCCCEEHHHEEECCCEEEEEEECCCCEEECCCCCCCHHHHHHHHEE
MSANDAAVTLAEKISGDVPHFVQRMNQEAQALGMKDTHFSNPAGITMPDHYTTAHDLSLL
EECCCHHEEEHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SQAVIHQTPEYLHYSKMPSFSYNQRFHHATNLALKYDPSVDGLKTGYTKAAGYNLALTAS
HHHHHHCCHHHHHHHCCCCCCCCCCHHHHHCEEEEECCCCCCCCCCCCCCCCCEEEEEEC
RPSFSPNLPQRRLLVIVLGTPSAVKRAEIADKLMNLAYAYTRDEVVIPEQKLIAELPVIK
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHH
STLKMFKVETKQPTIVTTSLYAEPTPIDLNTFDYATQRIQVLDSNQQPKVIAPLETTQTR
HHHHHHEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHEECCCCCCCEEECCCCCCEE
VNIQLNEQKLTAPLMKAMNLATVSIYQNNQLIRSLQIENDVHIEEANIFQRIMMWFSSLF
EEEEECHHHHHHHHHHHHCCEEEEEEECCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHH
SIFSSSEHSAAKLYPIDSH
HHHCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8955390; 9097040; 9278503 [H]