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The map label for this gene is rluE [H]

Identifier: 213158201

GI number: 213158201

Start: 3013402

End: 3014124

Strand: Direct

Name: rluE [H]

Synonym: AB57_2920

Alternate gene names: 213158201

Gene position: 3013402-3014124 (Clockwise)

Preceding gene: 213158199

Following gene: 213158204

Centisome position: 74.4

GC content: 44.54

Gene sequence:

>723_bases
ATGACGGTCTCTGACTTTGTAGATGACCCGACTTTGCGCATAGCAGGCCGTCTTGATATGGACTCTGAGGGTTTAGTTTT
CTTAACTGATCATGGTGGTTTGAATCAGTTCATTACCAACCCTGCGAATAAAAAATACAAAACGTATTTAGTTCAAGTTG
ATGGCGATGTAACCGAAGAAGCACTCGAGCAGCTTCGTAAGGGTGTAGAGCTTAATGATGGTATGACTTTACCAGCTAAA
GCAATTAAGGTAAGTGAGCCTGAATGGTTATGGGACCGCGATCCTCCTGTACGTTACCGTGCCAGCATTCCAACATCTTG
GATTGAAATCTCTATTTGTGAAGGTCGTAACCGCCAAGTTCGTCGCATGACCTCTGCGGTAGGTTTTCCGACTTTACGTC
TCATTCGTACAAAAATTGGTACGATTGACTTAGTTCAATTGGGATTACAACCGGGCGAAACCAAAGAAATTGAGCCTCTA
CTCTACCCAGACTTTAAAGATGTTCCGGCTGAACAACCTTATCGTTCACGTTCATATGTGAAAAAACCGGGTGGCACTGG
CGGTAAGCCAATGGTTCGCAAGAACAAAGATGGTTCTGCGAAAAAATCTGGTACAAAGCGTATTTGGCAAATGGATGAAA
GTGAAAAACCGCGTCGTAAGACTAACGGTACAACGCGTCCAAATACCAAAGCACCACGTGGTCGTAGCCGCAATAGCCGA
TAA

Upstream 100 bases:

>100_bases
TTTACCGCTTATTTGAAAGAAGAAGTCATTTTTTATGAAAATCGTCATTCTCAACAAACCATATGACGTTCTCTCCCAGT
TTCGTAAAGACGAAGCACAC

Downstream 100 bases:

>100_bases
AAATATTTAAGTTTTAAAGCTCACGTTAGTGGGCTTTTTTTAATAACTTTTATTTTATAAAAATAAAAAGCCGGTGCTTA
TGCACCGGCTTTTAGCTATG

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTVSDFVDDPTLRIAGRLDMDSEGLVFLTDHGGLNQFITNPANKKYKTYLVQVDGDVTEEALEQLRKGVELNDGMTLPAK
AIKVSEPEWLWDRDPPVRYRASIPTSWIEISICEGRNRQVRRMTSAVGFPTLRLIRTKIGTIDLVQLGLQPGETKEIEPL
LYPDFKDVPAEQPYRSRSYVKKPGGTGGKPMVRKNKDGSAKKSGTKRIWQMDESEKPRRKTNGTTRPNTKAPRGRSRNSR

Sequences:

>Translated_240_residues
MTVSDFVDDPTLRIAGRLDMDSEGLVFLTDHGGLNQFITNPANKKYKTYLVQVDGDVTEEALEQLRKGVELNDGMTLPAK
AIKVSEPEWLWDRDPPVRYRASIPTSWIEISICEGRNRQVRRMTSAVGFPTLRLIRTKIGTIDLVQLGLQPGETKEIEPL
LYPDFKDVPAEQPYRSRSYVKKPGGTGGKPMVRKNKDGSAKKSGTKRIWQMDESEKPRRKTNGTTRPNTKAPRGRSRNSR
>Mature_239_residues
TVSDFVDDPTLRIAGRLDMDSEGLVFLTDHGGLNQFITNPANKKYKTYLVQVDGDVTEEALEQLRKGVELNDGMTLPAKA
IKVSEPEWLWDRDPPVRYRASIPTSWIEISICEGRNRQVRRMTSAVGFPTLRLIRTKIGTIDLVQLGLQPGETKEIEPLL
YPDFKDVPAEQPYRSRSYVKKPGGTGGKPMVRKNKDGSAKKSGTKRIWQMDESEKPRRKTNGTTRPNTKAPRGRSRNSR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=156, Percent_Identity=55.1282051282051, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1788510, Length=159, Percent_Identity=37.1069182389937, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787524, Length=132, Percent_Identity=36.3636363636364, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790453, Length=145, Percent_Identity=32.4137931034483, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 27113; Mature: 26982

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSDFVDDPTLRIAGRLDMDSEGLVFLTDHGGLNQFITNPANKKYKTYLVQVDGDVTEE
CCCCHHCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHCCCCCCCEEEEEEEECCCHHHH
ALEQLRKGVELNDGMTLPAKAIKVSEPEWLWDRDPPVRYRASIPTSWIEISICEGRNRQV
HHHHHHCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHH
RRMTSAVGFPTLRLIRTKIGTIDLVQLGLQPGETKEIEPLLYPDFKDVPAEQPYRSRSYV
HHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHE
KKPGGTGGKPMVRKNKDGSAKKSGTKRIWQMDESEKPRRKTNGTTRPNTKAPRGRSRNSR
ECCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVSDFVDDPTLRIAGRLDMDSEGLVFLTDHGGLNQFITNPANKKYKTYLVQVDGDVTEE
CCCHHCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHCCCCCCCEEEEEEEECCCHHHH
ALEQLRKGVELNDGMTLPAKAIKVSEPEWLWDRDPPVRYRASIPTSWIEISICEGRNRQV
HHHHHHCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHH
RRMTSAVGFPTLRLIRTKIGTIDLVQLGLQPGETKEIEPLLYPDFKDVPAEQPYRSRSYV
HHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHE
KKPGGTGGKPMVRKNKDGSAKKSGTKRIWQMDESEKPRRKTNGTTRPNTKAPRGRSRNSR
ECCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA