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The map label for this gene is yneJ [H]

Identifier: 213158197

GI number: 213158197

Start: 3008370

End: 3009146

Strand: Reverse

Name: yneJ [H]

Synonym: AB57_2914

Alternate gene names: 213158197

Gene position: 3009146-3008370 (Counterclockwise)

Preceding gene: 213158200

Following gene: 213158196

Centisome position: 74.29

GC content: 39.0

Gene sequence:

>777_bases
GTGAAAAAGCTTGAAGAAGAATTAGGTGTGATTTTATTAAATCGTCATAACCCCGTTACATTAACCAATGCAGGACACCA
GCTACATAGTTATGCTGTGCAAATGATCGCACTGCATAATAAAGCGAAGACAATTTTCCGAGAAGGTGATATTGACCCGA
ATGAAACTATTCGTTTGGGCAGTATGGAAACGACAGCAGCAATACGACTTCCTCAATTGCTTAATGAATGTATGCAGCAA
CATCCGAATTTGCCGCTTGAACTTCAAACTCACCCGACAGGATATTTGTTAAATGCTGTGCAACAAGGAAAAGTCGATTG
TGCTTTTGTCGCTTCTAAATATGTGATTCCAGAGCTATATAGTTTTCCGGTGTGGCAAGAACAACTGGTTTTGGTTTGCC
CAAAACAACAAATTGATTTTCCCGACATTACCACCCTCGCAAAGACTCGATTTATTGCATTTCGACAAGGATGTTCCTAT
CGGCATGCAATTGAGTTGTTTTTAAATGATCACAGTGTGCCACCTTCGCAAATTATGGAAATGGGCAGTCTGGATGGCAT
TGTAAGCTGTGTGGAATTAGGTGTCGGTTTTGCTTTATTGCCAAAGTCATATCTGAATAAAGTAGCCGATAAAGTCTCAT
TAAGTTGTTTTGAAATTAACACTGAATCTGCACATTTCACTACTTATTTGGTCGCCCAGCCTCCTGAAACATGGGGCAGT
AATTTAAAGCAATTTATTTGTTTTATTGAGCAACAATTTATACAGAAATGCGCTTAA

Upstream 100 bases:

>100_bases
TTAAGTCTTTAGAGTTTTTTTTAGTCGGTTGCAGAGAGTGGAAGTTTTTCATTGGCTGCACAAAAAATAGTGTACGGTTC
AATCCAATATCACGAGCCAC

Downstream 100 bases:

>100_bases
TTTTTATACATAAATATCTAACGTAACTTATGTTTTAAAAGTGATATATATGTATTTAAAAGTCATTTTATATTCTCATT
CAGATCGGTAATGATCTGCA

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKKLEEELGVILLNRHNPVTLTNAGHQLHSYAVQMIALHNKAKTIFREGDIDPNETIRLGSMETTAAIRLPQLLNECMQQ
HPNLPLELQTHPTGYLLNAVQQGKVDCAFVASKYVIPELYSFPVWQEQLVLVCPKQQIDFPDITTLAKTRFIAFRQGCSY
RHAIELFLNDHSVPPSQIMEMGSLDGIVSCVELGVGFALLPKSYLNKVADKVSLSCFEINTESAHFTTYLVAQPPETWGS
NLKQFICFIEQQFIQKCA

Sequences:

>Translated_258_residues
MKKLEEELGVILLNRHNPVTLTNAGHQLHSYAVQMIALHNKAKTIFREGDIDPNETIRLGSMETTAAIRLPQLLNECMQQ
HPNLPLELQTHPTGYLLNAVQQGKVDCAFVASKYVIPELYSFPVWQEQLVLVCPKQQIDFPDITTLAKTRFIAFRQGCSY
RHAIELFLNDHSVPPSQIMEMGSLDGIVSCVELGVGFALLPKSYLNKVADKVSLSCFEINTESAHFTTYLVAQPPETWGS
NLKQFICFIEQQFIQKCA
>Mature_258_residues
MKKLEEELGVILLNRHNPVTLTNAGHQLHSYAVQMIALHNKAKTIFREGDIDPNETIRLGSMETTAAIRLPQLLNECMQQ
HPNLPLELQTHPTGYLLNAVQQGKVDCAFVASKYVIPELYSFPVWQEQLVLVCPKQQIDFPDITTLAKTRFIAFRQGCSY
RHAIELFLNDHSVPPSQIMEMGSLDGIVSCVELGVGFALLPKSYLNKVADKVSLSCFEINTESAHFTTYLVAQPPETWGS
NLKQFICFIEQQFIQKCA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=251, Percent_Identity=31.4741035856574, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1790208, Length=247, Percent_Identity=28.3400809716599, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=261, Percent_Identity=23.7547892720307, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 29061; Mature: 29061

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLEEELGVILLNRHNPVTLTNAGHQLHSYAVQMIALHNKAKTIFREGDIDPNETIRLG
CCCCHHHCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEC
SMETTAAIRLPQLLNECMQQHPNLPLELQTHPTGYLLNAVQQGKVDCAFVASKYVIPELY
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCEEEEHHHHHHHHHHH
SFPVWQEQLVLVCPKQQIDFPDITTLAKTRFIAFRQGCSYRHAIELFLNDHSVPPSQIME
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHEEEEECCCCCCHHHHHH
MGSLDGIVSCVELGVGFALLPKSYLNKVADKVSLSCFEINTESAHFTTYLVAQPPETWGS
HCCHHHHHHHHHHCCCHHHHCHHHHHHHHHHHEEEEEEECCCCCEEEEEEEECCCHHHHH
NLKQFICFIEQQFIQKCA
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLEEELGVILLNRHNPVTLTNAGHQLHSYAVQMIALHNKAKTIFREGDIDPNETIRLG
CCCCHHHCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEC
SMETTAAIRLPQLLNECMQQHPNLPLELQTHPTGYLLNAVQQGKVDCAFVASKYVIPELY
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCEEEEHHHHHHHHHHH
SFPVWQEQLVLVCPKQQIDFPDITTLAKTRFIAFRQGCSYRHAIELFLNDHSVPPSQIME
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHEEEEECCCCCCHHHHHH
MGSLDGIVSCVELGVGFALLPKSYLNKVADKVSLSCFEINTESAHFTTYLVAQPPETWGS
HCCHHHHHHHHHHCCCHHHHCHHHHHHHHHHHEEEEEEECCCCCEEEEEEEECCCHHHHH
NLKQFICFIEQQFIQKCA
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]