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The map label for this gene is ksgA [H]

Identifier: 213157955

GI number: 213157955

Start: 3560085

End: 3560897

Strand: Reverse

Name: ksgA [H]

Synonym: AB57_3449

Alternate gene names: 213157955

Gene position: 3560897-3560085 (Counterclockwise)

Preceding gene: 213157956

Following gene: 213157954

Centisome position: 87.91

GC content: 41.21

Gene sequence:

>813_bases
ATGTATCAAATTAATGCCCTAAACCCGAAAGATGAAGGGCATAAAGCTCGTAAACGTTTTGGTCAAAACTTCTTACATGA
TCAACGAGTCATTGCCAAAATTGTACGCTCAGTCAATCCGCGTACGGGTGATAATATCGTCGAAATTGGTCCAGGTTTAG
CTGCTTTAACTTCTCCTTTAATTGGAGAATGTGATGCATTAACTGTAGTTGAATTAGACCGTGATTTAGCTGCCGGCTTA
CCTGAACGCGTGCCACATCCAGAACGTTTAACTATCGTAGAAGCTGATGCATTAAAATATGATTTTAGTCAGCTTGTAAA
AGATGGGCGTCCATTACGCGTTGTCGGCAATTTGCCTTATAACATTTCTACACCATTACTTTTTCATCTCTTGGAATTTG
GTAGCCAAGTTAAAGACATGCACTTCATGTTGCAAAAAGAAGTGGTAGAACGTATTACTGCTGAACCGAATACAAAAGAA
TATGGCCGTTTATCAGTCATGATCCAATATTATTGTCAGCCTACCTTTTTATTTGAAGTTCCAGCAGGCGCATTTAACCC
GCCACCAAAAGTGACAAGTGCAGTATTTCGCTTGGTTCCTTACGAACAAAAACCAATTACGGCGAAAGATGAAAAAGCTT
TAGCTCGCTTGGTTGCCCACGTCTTTACTCAACGTCGTAAAACGCTAAGAAACAGTCTTAAAGGCATGTTGGCAGAGGAT
GGTTTCGAGAAAGCAGGAGTCGACCCAATGGCTCGTCCAGAAACTTTAACTCTTGCAGAGTTTGTGGCTTTGGCAGATCA
AATGGTGGCATAA

Upstream 100 bases:

>100_bases
TAGCTGGGACAGGATTGGCAAAAAGCTCAAGTTTAAATGTCGCTGTCGATTTAGCTTTATCCTTGGCAGCGAGTTAAATT
TTTCATGTTGGAAATGTTCT

Downstream 100 bases:

>100_bases
ATGACTAAGCGCTATAACTATATAATTGGAGATGTGCAAGGTTGTTTTGAGGCACTCAAAGCGCTATTAAAAGAAATACG
TTTTGACCCTGATCAAGATT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MYQINALNPKDEGHKARKRFGQNFLHDQRVIAKIVRSVNPRTGDNIVEIGPGLAALTSPLIGECDALTVVELDRDLAAGL
PERVPHPERLTIVEADALKYDFSQLVKDGRPLRVVGNLPYNISTPLLFHLLEFGSQVKDMHFMLQKEVVERITAEPNTKE
YGRLSVMIQYYCQPTFLFEVPAGAFNPPPKVTSAVFRLVPYEQKPITAKDEKALARLVAHVFTQRRKTLRNSLKGMLAED
GFEKAGVDPMARPETLTLAEFVALADQMVA

Sequences:

>Translated_270_residues
MYQINALNPKDEGHKARKRFGQNFLHDQRVIAKIVRSVNPRTGDNIVEIGPGLAALTSPLIGECDALTVVELDRDLAAGL
PERVPHPERLTIVEADALKYDFSQLVKDGRPLRVVGNLPYNISTPLLFHLLEFGSQVKDMHFMLQKEVVERITAEPNTKE
YGRLSVMIQYYCQPTFLFEVPAGAFNPPPKVTSAVFRLVPYEQKPITAKDEKALARLVAHVFTQRRKTLRNSLKGMLAED
GFEKAGVDPMARPETLTLAEFVALADQMVA
>Mature_270_residues
MYQINALNPKDEGHKARKRFGQNFLHDQRVIAKIVRSVNPRTGDNIVEIGPGLAALTSPLIGECDALTVVELDRDLAAGL
PERVPHPERLTIVEADALKYDFSQLVKDGRPLRVVGNLPYNISTPLLFHLLEFGSQVKDMHFMLQKEVVERITAEPNTKE
YGRLSVMIQYYCQPTFLFEVPAGAFNPPPKVTSAVFRLVPYEQKPITAKDEKALARLVAHVFTQRRKTLRNSLKGMLAED
GFEKAGVDPMARPETLTLAEFVALADQMVA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=279, Percent_Identity=34.0501792114695, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI7657198, Length=249, Percent_Identity=30.9236947791165, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1786236, Length=262, Percent_Identity=53.4351145038168, Blast_Score=281, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI25141369, Length=284, Percent_Identity=26.7605633802817, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25146882, Length=234, Percent_Identity=31.6239316239316, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=254, Percent_Identity=28.3464566929134, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357273, Length=277, Percent_Identity=32.8519855595668, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21358017, Length=241, Percent_Identity=31.9502074688797, Blast_Score=104, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30204; Mature: 30204

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQINALNPKDEGHKARKRFGQNFLHDQRVIAKIVRSVNPRTGDNIVEIGPGLAALTSPL
CEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCC
IGECDALTVVELDRDLAAGLPERVPHPERLTIVEADALKYDFSQLVKDGRPLRVVGNLPY
CCCCCCEEEEECCHHHHCCCHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCEEEECCCCC
NISTPLLFHLLEFGSQVKDMHFMLQKEVVERITAEPNTKEYGRLSVMIQYYCQPTFLFEV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEC
PAGAFNPPPKVTSAVFRLVPYEQKPITAKDEKALARLVAHVFTQRRKTLRNSLKGMLAED
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFEKAGVDPMARPETLTLAEFVALADQMVA
CCHHCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYQINALNPKDEGHKARKRFGQNFLHDQRVIAKIVRSVNPRTGDNIVEIGPGLAALTSPL
CEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCC
IGECDALTVVELDRDLAAGLPERVPHPERLTIVEADALKYDFSQLVKDGRPLRVVGNLPY
CCCCCCEEEEECCHHHHCCCHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCEEEECCCCC
NISTPLLFHLLEFGSQVKDMHFMLQKEVVERITAEPNTKEYGRLSVMIQYYCQPTFLFEV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEC
PAGAFNPPPKVTSAVFRLVPYEQKPITAKDEKALARLVAHVFTQRRKTLRNSLKGMLAED
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFEKAGVDPMARPETLTLAEFVALADQMVA
CCHHCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA