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The map label for this gene is lpdA [H]

Identifier: 213157647

GI number: 213157647

Start: 3246493

End: 3247926

Strand: Reverse

Name: lpdA [H]

Synonym: AB57_3124

Alternate gene names: 213157647

Gene position: 3247926-3246493 (Counterclockwise)

Preceding gene: 213157648

Following gene: 213157646

Centisome position: 80.19

GC content: 40.24

Gene sequence:

>1434_bases
ATGTCTCAACAATTTGATCTTGTTGTCATTGGCGGTGGTCCTGGTGGTTATGAAGCTGCGATTCGCGCTGCTCAACTAGG
CTTCAAAGTCGCTTGTATTGAAAAACGTATTCACAACGGTAAGCCTTCTTTAGGTGGTACTTGCTTAAACGTAGGTTGTA
TCCCTTCTAAAGCTTTGCTTGATTCTTCTCATCGTTATGAAGATACAGTGCATCATTTAGCTGATCACGGTATTACAACT
GGTGAAGTGAATTTCGATCTTGCAAAACTTCTTGCTCGTAAAGACAAAATTGTTGACCAATTAACTGGCGGTATTGATCA
ATTGCTTAAAGGCAACGGTATCGAGTGGTTAAAAGGTACAGGTAAATTACTTGCTGGTAAAAAAGTTGAGTTTGTTCCAC
ATGAAGGTGAAACTCAAATTTTAGAACCTAAATACGTAATTCTTGCGTCTGGTTCTGTGCCTGTAAATATTCCTGTAGCT
CCTGTAGATCAAGATATTATTGTTGATTCAACTGGCGCATTAAACTTCCCTGAAGTACCTAAGCGTTTAGGTGTAATTGG
TGCGGGTGTAATCGGTTTAGAACTTGGTTCAGTATGGCGTCGTCTTGGTGCTGAAGTTGTTGTATTTGAAGCAATGGATG
CATTCTTGCCAATGGCTGATAAAGCACTTTCTAAGGAATATCAAAAAATCTTGACTAAACAAGGTTTAGATATCCGCATT
GGTGCGAAAGTATCTGGTACTGAAGTAAACGGTCGTGAAGTGACTGTTAAATACACTCAAGCTGGTGAAGACAAAGAACA
AACGTTTGATAAGTTAATCGTTTGTGTTGGCCGTAAAGCTTATGCTGAAGGTTTATTGGCTGAAGATTCAGGTATTAAAT
TAACTGAACGTGGTTTAGTTGAAGTAAATGATCACTGTGCAACTTCTGTAGAAGGTGTATATGCGATTGGTGACTTAGTA
CGCGGTCCAATGCTTGCTCATAAAGCAATGGAAGAGGGCGTAATGGCTGTTGAACGTATTCATGGTCATGCAGCTCAAGT
AAACTATGACACAATCATTTCTGTTATCTATACACACCCTGAAGCGGCGTGGGTTGGTTTAACTGAAGAGCAAGCTAAAG
AAAAAGGTCATGAAGTAAAAACTGGTCAATTCGGTTTTGCTGTAAATGGTCGTGCTTTAGCTGCTGGTGAAGGTGCAGGT
TTTGTTAAGTTTGTTGCTGATGCAAAAACTGACCGTTTATTAGGTATGCATGTGATTGGGCCTGCGGCATCTGATATCGT
TCACCAAGGTATGATCGCACTTGAATTTGTATCTTCTGTTGAAGATCTTCAGTTGATGACTTTTGGTCACCCAACATTCT
CTGAAGTTGTTCATGAAGCTGCACTTGCTGTAGATGGTCGTGCAATTCACGCGATTCAACGTAAGCGTAAATAA

Upstream 100 bases:

>100_bases
TGTTAGAAGAACCAGCTAAACTCATTCTTGATCTTTAATTTCTTCGAATAAATACCTACCAGGGTAGAGTAATCCTCTAT
CCTGGTTTATGGAGATAAAA

Downstream 100 bases:

>100_bases
AATAAAGAGCGGTCCTCCGCTCTTTTTCACATTCGATGGTGTGATTAAAAAATCATCAAGTTGATAGACGTAGACAGCAA
TTTGCTTATACAACATGAGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSQQFDLVVIGGGPGGYEAAIRAAQLGFKVACIEKRIHNGKPSLGGTCLNVGCIPSKALLDSSHRYEDTVHHLADHGITT
GEVNFDLAKLLARKDKIVDQLTGGIDQLLKGNGIEWLKGTGKLLAGKKVEFVPHEGETQILEPKYVILASGSVPVNIPVA
PVDQDIIVDSTGALNFPEVPKRLGVIGAGVIGLELGSVWRRLGAEVVVFEAMDAFLPMADKALSKEYQKILTKQGLDIRI
GAKVSGTEVNGREVTVKYTQAGEDKEQTFDKLIVCVGRKAYAEGLLAEDSGIKLTERGLVEVNDHCATSVEGVYAIGDLV
RGPMLAHKAMEEGVMAVERIHGHAAQVNYDTIISVIYTHPEAAWVGLTEEQAKEKGHEVKTGQFGFAVNGRALAAGEGAG
FVKFVADAKTDRLLGMHVIGPAASDIVHQGMIALEFVSSVEDLQLMTFGHPTFSEVVHEAALAVDGRAIHAIQRKRK

Sequences:

>Translated_477_residues
MSQQFDLVVIGGGPGGYEAAIRAAQLGFKVACIEKRIHNGKPSLGGTCLNVGCIPSKALLDSSHRYEDTVHHLADHGITT
GEVNFDLAKLLARKDKIVDQLTGGIDQLLKGNGIEWLKGTGKLLAGKKVEFVPHEGETQILEPKYVILASGSVPVNIPVA
PVDQDIIVDSTGALNFPEVPKRLGVIGAGVIGLELGSVWRRLGAEVVVFEAMDAFLPMADKALSKEYQKILTKQGLDIRI
GAKVSGTEVNGREVTVKYTQAGEDKEQTFDKLIVCVGRKAYAEGLLAEDSGIKLTERGLVEVNDHCATSVEGVYAIGDLV
RGPMLAHKAMEEGVMAVERIHGHAAQVNYDTIISVIYTHPEAAWVGLTEEQAKEKGHEVKTGQFGFAVNGRALAAGEGAG
FVKFVADAKTDRLLGMHVIGPAASDIVHQGMIALEFVSSVEDLQLMTFGHPTFSEVVHEAALAVDGRAIHAIQRKRK
>Mature_476_residues
SQQFDLVVIGGGPGGYEAAIRAAQLGFKVACIEKRIHNGKPSLGGTCLNVGCIPSKALLDSSHRYEDTVHHLADHGITTG
EVNFDLAKLLARKDKIVDQLTGGIDQLLKGNGIEWLKGTGKLLAGKKVEFVPHEGETQILEPKYVILASGSVPVNIPVAP
VDQDIIVDSTGALNFPEVPKRLGVIGAGVIGLELGSVWRRLGAEVVVFEAMDAFLPMADKALSKEYQKILTKQGLDIRIG
AKVSGTEVNGREVTVKYTQAGEDKEQTFDKLIVCVGRKAYAEGLLAEDSGIKLTERGLVEVNDHCATSVEGVYAIGDLVR
GPMLAHKAMEEGVMAVERIHGHAAQVNYDTIISVIYTHPEAAWVGLTEEQAKEKGHEVKTGQFGFAVNGRALAAGEGAGF
VKFVADAKTDRLLGMHVIGPAASDIVHQGMIALEFVSSVEDLQLMTFGHPTFSEVVHEAALAVDGRAIHAIQRKRK

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=472, Percent_Identity=45.3389830508475, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI50301238, Length=467, Percent_Identity=26.1241970021413, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI148277065, Length=466, Percent_Identity=24.0343347639485, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519430, Length=466, Percent_Identity=24.0343347639485, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519428, Length=466, Percent_Identity=24.0343347639485, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519426, Length=466, Percent_Identity=24.0343347639485, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI148277071, Length=466, Percent_Identity=24.0343347639485, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI22035672, Length=461, Percent_Identity=26.0303687635575, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI291045266, Length=472, Percent_Identity=24.1525423728814, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI291045268, Length=464, Percent_Identity=22.8448275862069, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=38.0952380952381, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1789915, Length=459, Percent_Identity=28.1045751633987, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI87082354, Length=471, Percent_Identity=28.2377919320594, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=26.0303687635575, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI32565766, Length=475, Percent_Identity=45.8947368421053, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71983429, Length=382, Percent_Identity=29.8429319371728, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=26.1052631578947, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=382, Percent_Identity=29.8429319371728, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=479, Percent_Identity=24.0083507306889, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=485, Percent_Identity=44.1237113402062, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=28.8135593220339, Blast_Score=193, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6325166, Length=469, Percent_Identity=26.6524520255864, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21358499, Length=468, Percent_Identity=46.5811965811966, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=28.5412262156448, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24640553, Length=472, Percent_Identity=27.7542372881356, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24640549, Length=472, Percent_Identity=27.7542372881356, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=28.2105263157895, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51026; Mature: 50895

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS00098 THIOLASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQFDLVVIGGGPGGYEAAIRAAQLGFKVACIEKRIHNGKPSLGGTCLNVGCIPSKALL
CCCCEEEEEEECCCCCHHHHHHHHHHCEEHEEHHHHHHCCCCCCCCEEEEECCCCCHHHH
DSSHRYEDTVHHLADHGITTGEVNFDLAKLLARKDKIVDQLTGGIDQLLKGNGIEWLKGT
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEECCC
GKLLAGKKVEFVPHEGETQILEPKYVILASGSVPVNIPVAPVDQDIIVDSTGALNFPEVP
CCEECCCEEEEECCCCCCEEECCCEEEEECCCCEEEEECCCCCCCEEEECCCCCCCCHHH
KRLGVIGAGVIGLELGSVWRRLGAEVVVFEAMDAFLPMADKALSKEYQKILTKQGLDIRI
HHHHHHCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
GAKVSGTEVNGREVTVKYTQAGEDKEQTFDKLIVCVGRKAYAEGLLAEDSGIKLTERGLV
CCEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCHHHHCCCEECCCCCEEECCCCE
EVNDHCATSVEGVYAIGDLVRGPMLAHKAMEEGVMAVERIHGHAAQVNYDTIISVIYTHP
EECCCHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEECHHHEEEEEEECC
EAAWVGLTEEQAKEKGHEVKTGQFGFAVNGRALAAGEGAGFVKFVADAKTDRLLGMHVIG
CCEEECCCHHHHHHCCCCEECCCEEEEECCEEEECCCCCCEEEEEECCCCCCEEEEEEEC
PAASDIVHQGMIALEFVSSVEDLQLMTFGHPTFSEVVHEAALAVDGRAIHAIQRKRK
CCHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure 
SQQFDLVVIGGGPGGYEAAIRAAQLGFKVACIEKRIHNGKPSLGGTCLNVGCIPSKALL
CCCEEEEEEECCCCCHHHHHHHHHHCEEHEEHHHHHHCCCCCCCCEEEEECCCCCHHHH
DSSHRYEDTVHHLADHGITTGEVNFDLAKLLARKDKIVDQLTGGIDQLLKGNGIEWLKGT
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEECCC
GKLLAGKKVEFVPHEGETQILEPKYVILASGSVPVNIPVAPVDQDIIVDSTGALNFPEVP
CCEECCCEEEEECCCCCCEEECCCEEEEECCCCEEEEECCCCCCCEEEECCCCCCCCHHH
KRLGVIGAGVIGLELGSVWRRLGAEVVVFEAMDAFLPMADKALSKEYQKILTKQGLDIRI
HHHHHHCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
GAKVSGTEVNGREVTVKYTQAGEDKEQTFDKLIVCVGRKAYAEGLLAEDSGIKLTERGLV
CCEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCHHHHCCCEECCCCCEEECCCCE
EVNDHCATSVEGVYAIGDLVRGPMLAHKAMEEGVMAVERIHGHAAQVNYDTIISVIYTHP
EECCCHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEECHHHEEEEEEECC
EAAWVGLTEEQAKEKGHEVKTGQFGFAVNGRALAAGEGAGFVKFVADAKTDRLLGMHVIG
CCEEECCCHHHHHHCCCCEECCCEEEEECCEEEECCCCCCEEEEEECCCCCCEEEEEEEC
PAASDIVHQGMIALEFVSSVEDLQLMTFGHPTFSEVVHEAALAVDGRAIHAIQRKRK
CCHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1902462; 2914869 [H]