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The map label for this gene is sucB [H]

Identifier: 213157648

GI number: 213157648

Start: 3247989

End: 3249185

Strand: Reverse

Name: sucB [H]

Synonym: AB57_3125

Alternate gene names: 213157648

Gene position: 3249185-3247989 (Counterclockwise)

Preceding gene: 213157649

Following gene: 213157647

Centisome position: 80.22

GC content: 43.27

Gene sequence:

>1197_bases
ATGGCAACCGAAATTAAAGCACCGGTATTCCCAGAGTCTGTTGCAGATGGAACCATCGCAACTTGGCATAAAAAGGTGGG
TGAACCTGTATCACGTGATGAAGTAATCTGTGATATTGAAACCGACAAAGTTGTTTTAGAAGTTGTTGCTCCTGCAGATG
GTAGCTTGGTTGCAATCATTAAAGGTGAAGGCGATACAGTTCTTTCTGACGAAGTTATTGCTCAATTCGAAGCTGGTGCT
GGCGCAGCGGCTGCTGCTCCTGCGGCTGTAGAACAAGCAGTTGCTCAGACTCAAGCTGGTGCTGCTCCTGTTGTTGAGCG
TAATGAAGCTGTATCTGATCAAGCTCCTGCGGTACGTAAAGCGTTAACTGAATCTGGTATTGCTGCTTCTGACGTACAAG
GTACTGGTCGTGGTGGTCGTATCACTAAAGAAGATGTTGCGAACCATCAAGCTAAACCAGCTGCTAACGTTACTCCATTA
AGCGTTGCTGTTGGCGAGCGTATCGAAAAACGCGTTCCAATGACTCGTTTACGTAAACGTGTAGCTGAGCGTCTTCTTGC
GGCTACTCAAGAAACAGCAATGTTAACTACATTTAACGAAGTTAACATGAAGCCAATCATGGAATTACGTAAGCAATATA
AAGATGCTTTCGAAAAACGTCATGGTGCTCGTTTAGGCTTCATGTCATTCTTCGTTAAAGCAGCAACTGAAGCGCTTAAA
CGCTACCCAGCTGTAAATGCTTCAATTGATGGCGATGATATCGTTTATCACGGTTACTATGACATCGGTGTTGCAGTATC
TTCTGATCGTGGTCTTGTTGTACCAGTATTACGTGATACTGACCGTATGAGCTATGCAGAAGTTGAAGCAGGTATTGCTG
CTTACGCTGCTAAAGCACGTGATGGTAAATTATCTATCGAAGAAATGACTGGCGGTACTTTCACAATCACTAACGGTGGT
ACTTTCGGTTCATTACTTTCAACTCCAATTTTGAACCAGCCGCAAACTGGTATCTTGGGTATGCATAAAATCCAAGAACG
TCCTATGGCGGTAAATGGTCAAGTTGAAATCTTGCCAATGATGTACTTAGCGCTTTCTTATGACCACCGTATGATCGATG
GTAAAGAAGCTGTAGGTTTCTTAGTAGCAATCAAAGAATTGTTAGAAGAACCAGCTAAACTCATTCTTGATCTTTAA

Upstream 100 bases:

>100_bases
CACCAGCTTGTGGCTCACCGTACTTGCATGCAAAACAACAAGCTCAGCTAATTAATGATGCATTGGCAATCGAAGCTGAA
CAATCAGGAGATTCTCAATA

Downstream 100 bases:

>100_bases
TTTCTTCGAATAAATACCTACCAGGGTAGAGTAATCCTCTATCCTGGTTTATGGAGATAAAAATGTCTCAACAATTTGAT
CTTGTTGTCATTGGCGGTGG

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MATEIKAPVFPESVADGTIATWHKKVGEPVSRDEVICDIETDKVVLEVVAPADGSLVAIIKGEGDTVLSDEVIAQFEAGA
GAAAAAPAAVEQAVAQTQAGAAPVVERNEAVSDQAPAVRKALTESGIAASDVQGTGRGGRITKEDVANHQAKPAANVTPL
SVAVGERIEKRVPMTRLRKRVAERLLAATQETAMLTTFNEVNMKPIMELRKQYKDAFEKRHGARLGFMSFFVKAATEALK
RYPAVNASIDGDDIVYHGYYDIGVAVSSDRGLVVPVLRDTDRMSYAEVEAGIAAYAAKARDGKLSIEEMTGGTFTITNGG
TFGSLLSTPILNQPQTGILGMHKIQERPMAVNGQVEILPMMYLALSYDHRMIDGKEAVGFLVAIKELLEEPAKLILDL

Sequences:

>Translated_398_residues
MATEIKAPVFPESVADGTIATWHKKVGEPVSRDEVICDIETDKVVLEVVAPADGSLVAIIKGEGDTVLSDEVIAQFEAGA
GAAAAAPAAVEQAVAQTQAGAAPVVERNEAVSDQAPAVRKALTESGIAASDVQGTGRGGRITKEDVANHQAKPAANVTPL
SVAVGERIEKRVPMTRLRKRVAERLLAATQETAMLTTFNEVNMKPIMELRKQYKDAFEKRHGARLGFMSFFVKAATEALK
RYPAVNASIDGDDIVYHGYYDIGVAVSSDRGLVVPVLRDTDRMSYAEVEAGIAAYAAKARDGKLSIEEMTGGTFTITNGG
TFGSLLSTPILNQPQTGILGMHKIQERPMAVNGQVEILPMMYLALSYDHRMIDGKEAVGFLVAIKELLEEPAKLILDL
>Mature_397_residues
ATEIKAPVFPESVADGTIATWHKKVGEPVSRDEVICDIETDKVVLEVVAPADGSLVAIIKGEGDTVLSDEVIAQFEAGAG
AAAAAPAAVEQAVAQTQAGAAPVVERNEAVSDQAPAVRKALTESGIAASDVQGTGRGGRITKEDVANHQAKPAANVTPLS
VAVGERIEKRVPMTRLRKRVAERLLAATQETAMLTTFNEVNMKPIMELRKQYKDAFEKRHGARLGFMSFFVKAATEALKR
YPAVNASIDGDDIVYHGYYDIGVAVSSDRGLVVPVLRDTDRMSYAEVEAGIAAYAAKARDGKLSIEEMTGGTFTITNGGT
FGSLLSTPILNQPQTGILGMHKIQERPMAVNGQVEILPMMYLALSYDHRMIDGKEAVGFLVAIKELLEEPAKLILDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=236, Percent_Identity=55.0847457627119, Blast_Score=271, Evalue=9e-73,
Organism=Homo sapiens, GI110671329, Length=416, Percent_Identity=26.9230769230769, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI31711992, Length=430, Percent_Identity=27.2093023255814, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=26.3858093126386, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=26.3858093126386, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.7826086956522, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1786946, Length=406, Percent_Identity=61.0837438423645, Blast_Score=490, Evalue=1e-140,
Organism=Escherichia coli, GI1786305, Length=407, Percent_Identity=30.4668304668305, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=405, Percent_Identity=41.9753086419753, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17560088, Length=428, Percent_Identity=28.7383177570093, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=418, Percent_Identity=25.3588516746411, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=325, Percent_Identity=28.9230769230769, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=406, Percent_Identity=43.8423645320197, Blast_Score=331, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=456, Percent_Identity=25.6578947368421, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24645909, Length=235, Percent_Identity=56.5957446808511, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI18859875, Length=418, Percent_Identity=26.3157894736842, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=30.5676855895196, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=30.5676855895196, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42515; Mature: 42384

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEIKAPVFPESVADGTIATWHKKVGEPVSRDEVICDIETDKVVLEVVAPADGSLVAII
CCCCCCCCCCCCHHCCCCHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCCCCEEEEE
KGEGDTVLSDEVIAQFEAGAGAAAAAPAAVEQAVAQTQAGAAPVVERNEAVSDQAPAVRK
ECCCCCEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
ALTESGIAASDVQGTGRGGRITKEDVANHQAKPAANVTPLSVAVGERIEKRVPMTRLRKR
HHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHH
VAERLLAATQETAMLTTFNEVNMKPIMELRKQYKDAFEKRHGARLGFMSFFVKAATEALK
HHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RYPAVNASIDGDDIVYHGYYDIGVAVSSDRGLVVPVLRDTDRMSYAEVEAGIAAYAAKAR
HCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
DGKLSIEEMTGGTFTITNGGTFGSLLSTPILNQPQTGILGMHKIQERPMAVNGQVEILPM
CCCEEEEEECCCEEEEECCCCHHHHHCCCCCCCCCCCCCCHHHHHCCCCEECCCEEHHHH
MYLALSYDHRMIDGKEAVGFLVAIKELLEEPAKLILDL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATEIKAPVFPESVADGTIATWHKKVGEPVSRDEVICDIETDKVVLEVVAPADGSLVAII
CCCCCCCCCCCHHCCCCHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCCCCEEEEE
KGEGDTVLSDEVIAQFEAGAGAAAAAPAAVEQAVAQTQAGAAPVVERNEAVSDQAPAVRK
ECCCCCEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
ALTESGIAASDVQGTGRGGRITKEDVANHQAKPAANVTPLSVAVGERIEKRVPMTRLRKR
HHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHH
VAERLLAATQETAMLTTFNEVNMKPIMELRKQYKDAFEKRHGARLGFMSFFVKAATEALK
HHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RYPAVNASIDGDDIVYHGYYDIGVAVSSDRGLVVPVLRDTDRMSYAEVEAGIAAYAAKAR
HCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
DGKLSIEEMTGGTFTITNGGTFGSLLSTPILNQPQTGILGMHKIQERPMAVNGQVEILPM
CCCEEEEEECCCEEEEECCCCHHHHHCCCCCCCCCCCCCCHHHHHCCCCEECCCEEHHHH
MYLALSYDHRMIDGKEAVGFLVAIKELLEEPAKLILDL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]