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The map label for this gene is yngF [H]

Identifier: 213157130

GI number: 213157130

Start: 1896857

End: 1897978

Strand: Direct

Name: yngF [H]

Synonym: AB57_1813

Alternate gene names: 213157130

Gene position: 1896857-1897978 (Clockwise)

Preceding gene: 213157129

Following gene: 213157131

Centisome position: 46.83

GC content: 41.44

Gene sequence:

>1122_bases
ATGAACTCTCCCAATGTAAATAGCTATCATCCGGATTTGGTAGTTGAAGAAGCGAAAAATGGTTGGCGTATTGTACGTTT
GAATCGACCTAAATCATTACACGCTTTAGATGAATCAATCGTAACGGCTCTATTACGAGTATTTGAAGACTTCCGTGATG
ATGAGCAAGTAAAAGCAATTTGGTTGGACTCGACTACACCAAAAGCATTTTGTGCGGGTGGAGATGTACGCAAATTACGT
CAGCTGGTAATTAATCAGGAAGTAGAAACCGCAAATAAATTTTTCCAGCAAGAATATGCTTTGGATTTATTGTTACATAA
CTATGCTAAACCGGTTGTTGTTTGGGGCGAAGGCTATGTAATGGGTGGTGGTTTAGGCTTGTTTATGGCTGCACCTTTCC
GTTTGGTTACACCATATTCACGTTTGGCTATGCCTGAAATTAATATTGGTTTATACCCTGATGTTGGAGCAAGCCGTTTC
CTTGCTGACCGTGGGCAAATTGGTTTATTTACTGGTTTGACAGGTTCTATTATGACGGCTGCTGGGGCATATAGCATTGG
TTGGGCGACGCATATTTGTGAAGCACAACGTGATAATGTTCTACAGAAAGTACTCAATATTAATTGGGCTCACTATCCGG
CAGGTGATTTCCGTGCAATCGATGATACTTTAAATAGTTTACATCGACCGGTTGCAGCAGGGCCATTACAAAATTCGTTA
GACGTTATACATAGTGTTTGCCGTGGTTCAAGCTTTGAACAAGACTATCAAGCGATCGTCAGCTTGCGAGATGCAAGCAG
TGACTGGTTGCGTCAAGCGAGTGAAAACTTACAAAAGGGTTCGCCTAGTACAGCAGCAATTACTTGGTTGTTATGGCAAT
GGGGTAAACAAGTTCATTCTTGGGATGAAGTTTTTGATTTAGAAGCACAAATCTCTGAATGGAAAATTCGACATCCTGAC
TTTGTGGAAGGCGTACGTGCTCGTTTAGTCGATAAAGATTTATCGCCAGAATGGAAAGCATGTGAAGACCTTACATTACG
TGGTATTTTGGCCGATGATCCACCGATCACGACGATTGATAGCTGGAATGCATTACTTAGACAATATGGTGTAGTGTCAT
AA

Upstream 100 bases:

>100_bases
CTAATCGTGGTGGTTTCTTTGGATGCAAAGTGTTTTCCAAAACAAATCAATATTTGAAACAACATGGCATTGAGCCCATA
GACTGGCAATTGGACGCATG

Downstream 100 bases:

>100_bases
TCAATAATGTCTAAATTTAGTGATGAGTATTGGATGCAGCTTGCTTACGAGCAAGCTGAATTAGCAGCCAAGCAGGGGGA
AATTCCTGTAGGAGCTGTAA

Product: putative enoyl-CoA hydratase/isomerase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNSPNVNSYHPDLVVEEAKNGWRIVRLNRPKSLHALDESIVTALLRVFEDFRDDEQVKAIWLDSTTPKAFCAGGDVRKLR
QLVINQEVETANKFFQQEYALDLLLHNYAKPVVVWGEGYVMGGGLGLFMAAPFRLVTPYSRLAMPEINIGLYPDVGASRF
LADRGQIGLFTGLTGSIMTAAGAYSIGWATHICEAQRDNVLQKVLNINWAHYPAGDFRAIDDTLNSLHRPVAAGPLQNSL
DVIHSVCRGSSFEQDYQAIVSLRDASSDWLRQASENLQKGSPSTAAITWLLWQWGKQVHSWDEVFDLEAQISEWKIRHPD
FVEGVRARLVDKDLSPEWKACEDLTLRGILADDPPITTIDSWNALLRQYGVVS

Sequences:

>Translated_373_residues
MNSPNVNSYHPDLVVEEAKNGWRIVRLNRPKSLHALDESIVTALLRVFEDFRDDEQVKAIWLDSTTPKAFCAGGDVRKLR
QLVINQEVETANKFFQQEYALDLLLHNYAKPVVVWGEGYVMGGGLGLFMAAPFRLVTPYSRLAMPEINIGLYPDVGASRF
LADRGQIGLFTGLTGSIMTAAGAYSIGWATHICEAQRDNVLQKVLNINWAHYPAGDFRAIDDTLNSLHRPVAAGPLQNSL
DVIHSVCRGSSFEQDYQAIVSLRDASSDWLRQASENLQKGSPSTAAITWLLWQWGKQVHSWDEVFDLEAQISEWKIRHPD
FVEGVRARLVDKDLSPEWKACEDLTLRGILADDPPITTIDSWNALLRQYGVVS
>Mature_373_residues
MNSPNVNSYHPDLVVEEAKNGWRIVRLNRPKSLHALDESIVTALLRVFEDFRDDEQVKAIWLDSTTPKAFCAGGDVRKLR
QLVINQEVETANKFFQQEYALDLLLHNYAKPVVVWGEGYVMGGGLGLFMAAPFRLVTPYSRLAMPEINIGLYPDVGASRF
LADRGQIGLFTGLTGSIMTAAGAYSIGWATHICEAQRDNVLQKVLNINWAHYPAGDFRAIDDTLNSLHRPVAAGPLQNSL
DVIHSVCRGSSFEQDYQAIVSLRDASSDWLRQASENLQKGSPSTAAITWLLWQWGKQVHSWDEVFDLEAQISEWKIRHPD
FVEGVRARLVDKDLSPEWKACEDLTLRGILADDPPITTIDSWNALLRQYGVVS

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=345, Percent_Identity=31.5942028985507, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI37594469, Length=319, Percent_Identity=29.153605015674, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194097323, Length=181, Percent_Identity=30.939226519337, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=322, Percent_Identity=32.9192546583851, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25144160, Length=322, Percent_Identity=32.9192546583851, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17554946, Length=202, Percent_Identity=27.7227722772277, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=197, Percent_Identity=24.8730964467005, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=378, Percent_Identity=26.984126984127, Blast_Score=145, Evalue=7e-36,
Organism=Drosophila melanogaster, GI28571730, Length=338, Percent_Identity=32.5443786982249, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI28571729, Length=338, Percent_Identity=32.5443786982249, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24653139, Length=202, Percent_Identity=23.7623762376238, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 41806; Mature: 41806

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPNVNSYHPDLVVEEAKNGWRIVRLNRPKSLHALDESIVTALLRVFEDFRDDEQVKAI
CCCCCCCCCCCCHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
WLDSTTPKAFCAGGDVRKLRQLVINQEVETANKFFQQEYALDLLLHNYAKPVVVWGEGYV
EECCCCCCCEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
MGGGLGLFMAAPFRLVTPYSRLAMPEINIGLYPDVGASRFLADRGQIGLFTGLTGSIMTA
EECCHHHHHHCCHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHH
AGAYSIGWATHICEAQRDNVLQKVLNINWAHYPAGDFRAIDDTLNSLHRPVAAGPLQNSL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHH
DVIHSVCRGSSFEQDYQAIVSLRDASSDWLRQASENLQKGSPSTAAITWLLWQWGKQVHS
HHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
WDEVFDLEAQISEWKIRHPDFVEGVRARLVDKDLSPEWKACEDLTLRGILADDPPITTID
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEECCCCCCCCHH
SWNALLRQYGVVS
HHHHHHHHHCCCC
>Mature Secondary Structure
MNSPNVNSYHPDLVVEEAKNGWRIVRLNRPKSLHALDESIVTALLRVFEDFRDDEQVKAI
CCCCCCCCCCCCHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
WLDSTTPKAFCAGGDVRKLRQLVINQEVETANKFFQQEYALDLLLHNYAKPVVVWGEGYV
EECCCCCCCEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
MGGGLGLFMAAPFRLVTPYSRLAMPEINIGLYPDVGASRFLADRGQIGLFTGLTGSIMTA
EECCHHHHHHCCHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHH
AGAYSIGWATHICEAQRDNVLQKVLNINWAHYPAGDFRAIDDTLNSLHRPVAAGPLQNSL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHH
DVIHSVCRGSSFEQDYQAIVSLRDASSDWLRQASENLQKGSPSTAAITWLLWQWGKQVHS
HHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
WDEVFDLEAQISEWKIRHPDFVEGVRARLVDKDLSPEWKACEDLTLRGILADDPPITTID
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEECCCCCCCCHH
SWNALLRQYGVVS
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]