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The map label for this gene is mucK [H]

Identifier: 213156916

GI number: 213156916

Start: 1673136

End: 1674419

Strand: Direct

Name: mucK [H]

Synonym: AB57_1587

Alternate gene names: 213156916

Gene position: 1673136-1674419 (Clockwise)

Preceding gene: 213156915

Following gene: 213156917

Centisome position: 41.31

GC content: 40.11

Gene sequence:

>1284_bases
ATGACAACAAAGACTGTTACAGAGAATATGAGTAGCACTGAAGCTATTCAAACACCAAAAAAGATTTGGATTACAGCTTT
TATATTCGCTTTTTTTACACTTTTATGTGACGGGGCCGACTTAGGTTTTTTGGCATTAAGCCTCACTAGTCTTAAAGCAG
AGTTTCATTTAACTGGTGTGCAAGCCGGAACATTAGGAAGCTTGACACTATTTGGTTCGGCCGTGGGTGGTCTTATTGGT
GGTTGGGCTTGCGACCGTTTTGGTCGGGTCCGCATTATTGTTTTCTTTATTGCTTTTTCTTCGATATTAACCTGTGCACT
CGGCTTTACACATTCTTATATGCAATTTGCAGTTTTAAGAACAATAGGCGCCATGGGCTTAGGTGCATTATATATTGCCT
GTAACATTCTTATGTCTGAAATGGTGCCAACCAAACACCGTTCTACCGTTTTAGCAACGCTGATGACAGGTTATACCCTA
GGTTCTTTATTAGCGACATTACTAGCAGGTCATATTATTCCTGAACATGGCTGGCGTTTCCTATACTGGATTGCAATTAC
CCCTGTGGTTTTGTCAGTTTTGATGCACTTTTGTGTGCCTGAACCTGAGTCATGGAAAAAAGCCCGTCAACTAAAAGCGC
TAGAAGTCGCTCAAAGTAACCAACCTAAAAAACGTCAAAATCCATATCTTGAGATTTTAAGAGATAAAAAACACGGCACC
ATGTTTATTCTTTGGATTATTAGTACTGGGGCATTGCAGTTTGGTTATTACGGCGTAAGTAACTGGTTACCTGCTTATTT
AGAGTCTGACCTTGGCATCAAGTTTAAAGAAATGGCCATGTATATGGTCGGTACATTCCTAATTATGATGTTTGCCAAAG
TGATCGCAGGGATCGTTGCCGATAAATTAGGACGCCGCGCTGTATTTGCTTTCGGAACCATTGGTACAGCCTTATTTATT
CCAGTCATTGTTTATTTAAATACACCAACCAACATTTTATGGATGATGCTGTTCTTCGGTTTCCTTTACGGTATTCCATA
TGCAATTAATGCCACCTATATGACAGAAAGCTTCCCGACTTCAATTCGAGGCTCGGCAGTGGGCGGTGCTTATAACATTG
GCAAAGTACTTTCAATTTTCTCTCCATTAACAATTGGTTATCTATCGCAAAACGGTTCTATTGGTTTAGGACTCTTGGTG
ATGGCTGCAGCTTATTTTATCTGCGGTGTAATTCCATTGTTATTTATTAAAGACCGATTATTTAACCCGCAAAAGGCAGA
GTGA

Upstream 100 bases:

>100_bases
CTTATTATTGCCAAAAACATGATTAGGGAAGCTACAGCCTAATAAAAAATCAAATGCCATGAGGCATGGGATGCCTCATG
TTCCTTCTGAGGATATGGAC

Downstream 100 bases:

>100_bases
CAGATCAATCTGTCACCTTAAATTAAGGATGAAAAGGGACAAGTGAGATGATAAATAAAATCACCAGTGACATTGAACCG
ATTTTAAAAGCTATTCCTGA

Product: MFS short chain dicaboxylate:H+ symporter

Products: Proton [Cytoplasm]; N-acetylneuraminate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTTKTVTENMSSTEAIQTPKKIWITAFIFAFFTLLCDGADLGFLALSLTSLKAEFHLTGVQAGTLGSLTLFGSAVGGLIG
GWACDRFGRVRIIVFFIAFSSILTCALGFTHSYMQFAVLRTIGAMGLGALYIACNILMSEMVPTKHRSTVLATLMTGYTL
GSLLATLLAGHIIPEHGWRFLYWIAITPVVLSVLMHFCVPEPESWKKARQLKALEVAQSNQPKKRQNPYLEILRDKKHGT
MFILWIISTGALQFGYYGVSNWLPAYLESDLGIKFKEMAMYMVGTFLIMMFAKVIAGIVADKLGRRAVFAFGTIGTALFI
PVIVYLNTPTNILWMMLFFGFLYGIPYAINATYMTESFPTSIRGSAVGGAYNIGKVLSIFSPLTIGYLSQNGSIGLGLLV
MAAAYFICGVIPLLFIKDRLFNPQKAE

Sequences:

>Translated_427_residues
MTTKTVTENMSSTEAIQTPKKIWITAFIFAFFTLLCDGADLGFLALSLTSLKAEFHLTGVQAGTLGSLTLFGSAVGGLIG
GWACDRFGRVRIIVFFIAFSSILTCALGFTHSYMQFAVLRTIGAMGLGALYIACNILMSEMVPTKHRSTVLATLMTGYTL
GSLLATLLAGHIIPEHGWRFLYWIAITPVVLSVLMHFCVPEPESWKKARQLKALEVAQSNQPKKRQNPYLEILRDKKHGT
MFILWIISTGALQFGYYGVSNWLPAYLESDLGIKFKEMAMYMVGTFLIMMFAKVIAGIVADKLGRRAVFAFGTIGTALFI
PVIVYLNTPTNILWMMLFFGFLYGIPYAINATYMTESFPTSIRGSAVGGAYNIGKVLSIFSPLTIGYLSQNGSIGLGLLV
MAAAYFICGVIPLLFIKDRLFNPQKAE
>Mature_426_residues
TTKTVTENMSSTEAIQTPKKIWITAFIFAFFTLLCDGADLGFLALSLTSLKAEFHLTGVQAGTLGSLTLFGSAVGGLIGG
WACDRFGRVRIIVFFIAFSSILTCALGFTHSYMQFAVLRTIGAMGLGALYIACNILMSEMVPTKHRSTVLATLMTGYTLG
SLLATLLAGHIIPEHGWRFLYWIAITPVVLSVLMHFCVPEPESWKKARQLKALEVAQSNQPKKRQNPYLEILRDKKHGTM
FILWIISTGALQFGYYGVSNWLPAYLESDLGIKFKEMAMYMVGTFLIMMFAKVIAGIVADKLGRRAVFAFGTIGTALFIP
VIVYLNTPTNILWMMLFFGFLYGIPYAINATYMTESFPTSIRGSAVGGAYNIGKVLSIFSPLTIGYLSQNGSIGLGLLVM
AAAYFICGVIPLLFIKDRLFNPQKAE

Specific function: Probable uptake of muconate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI213021148, Length=419, Percent_Identity=22.4343675417661, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI87082231, Length=439, Percent_Identity=24.6013667425968, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87082404, Length=400, Percent_Identity=23, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1790550, Length=380, Percent_Identity=22.3684210526316, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786229, Length=388, Percent_Identity=21.9072164948454, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082159, Length=188, Percent_Identity=28.7234042553192, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081723, Length=399, Percent_Identity=23.8095238095238, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789152, Length=445, Percent_Identity=21.123595505618, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788074, Length=419, Percent_Identity=24.3436754176611, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115534558, Length=378, Percent_Identity=24.6031746031746, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24584153, Length=246, Percent_Identity=24.390243902439, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI221330907, Length=350, Percent_Identity=22.8571428571429, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24668440, Length=355, Percent_Identity=23.0985915492958, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46906; Mature: 46775

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKTVTENMSSTEAIQTPKKIWITAFIFAFFTLLCDGADLGFLALSLTSLKAEFHLTGV
CCCCCHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEECC
QAGTLGSLTLFGSAVGGLIGGWACDRFGRVRIIVFFIAFSSILTCALGFTHSYMQFAVLR
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIGAMGLGALYIACNILMSEMVPTKHRSTVLATLMTGYTLGSLLATLLAGHIIPEHGWRF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
LYWIAITPVVLSVLMHFCVPEPESWKKARQLKALEVAQSNQPKKRQNPYLEILRDKKHGT
EHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCC
MFILWIISTGALQFGYYGVSNWLPAYLESDLGIKFKEMAMYMVGTFLIMMFAKVIAGIVA
EEEEEEECCCCHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKLGRRAVFAFGTIGTALFIPVIVYLNTPTNILWMMLFFGFLYGIPYAINATYMTESFPT
HHHCCCEEEEHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
SIRGSAVGGAYNIGKVLSIFSPLTIGYLSQNGSIGLGLLVMAAAYFICGVIPLLFIKDRL
CCCCCCCCCCHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
FNPQKAE
CCCCCCC
>Mature Secondary Structure 
TTKTVTENMSSTEAIQTPKKIWITAFIFAFFTLLCDGADLGFLALSLTSLKAEFHLTGV
CCCCHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEECC
QAGTLGSLTLFGSAVGGLIGGWACDRFGRVRIIVFFIAFSSILTCALGFTHSYMQFAVLR
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIGAMGLGALYIACNILMSEMVPTKHRSTVLATLMTGYTLGSLLATLLAGHIIPEHGWRF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
LYWIAITPVVLSVLMHFCVPEPESWKKARQLKALEVAQSNQPKKRQNPYLEILRDKKHGT
EHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCC
MFILWIISTGALQFGYYGVSNWLPAYLESDLGIKFKEMAMYMVGTFLIMMFAKVIAGIVA
EEEEEEECCCCHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKLGRRAVFAFGTIGTALFIPVIVYLNTPTNILWMMLFFGFLYGIPYAINATYMTESFPT
HHHCCCEEEEHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
SIRGSAVGGAYNIGKVLSIFSPLTIGYLSQNGSIGLGLLVMAAAYFICGVIPLLFIKDRL
CCCCCCCCCCHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
FNPQKAE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; N-acetylneuraminate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + N-acetylneuraminate [Periplasm] = Proton [Cytoplasm] + N-acetylneuraminate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9294455 [H]