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The map label for this gene is kgtP [H]

Identifier: 21245020

GI number: 21245020

Start: 5094404

End: 5095765

Strand: Reverse

Name: kgtP [H]

Synonym: XAC4308

Alternate gene names: 21245020

Gene position: 5095765-5094404 (Counterclockwise)

Preceding gene: 21245023

Following gene: 21245014

Centisome position: 98.46

GC content: 63.22

Gene sequence:

>1362_bases
ATGAGCGCACCCCCATCGTCTCCGATCGCTGCCGGTTCTGGCACCGCTGCCGGTAGCCTTCGCCGCTCGGTGTCCAACAC
GCTCAAGGGCTCGGCCGGCAACCTCGTCGAGTGGTACGACGTCTACGTCTATTCGGTGTTCGCCACCTACTTCGAGTCGC
AGTTCTTCTCCAAGGAAGACAAGAACGCCACCATGTTCGTGTGGGCGATCTTCGCGATGACGTTCCTGATGCGCCCGATC
GGCGCCTGGTATTTCGGCCGTTTCGCCGACCGTTATGGCCGCCGCCTGGCGTTGACCATTTCGGTGTCGGTGATGGCGCT
GTGCTCGTTCGTCATTGCGATCACGCCCACGGTGGCCACGATCGGCATCGCTGCGCCGATCATCTTGCTGGTGGCGCGCC
TGCTGCAGGGCTTTGCGACCGGTGGCGAATACGGCACCAGCGCCACGTACATGTCCGAAGCGGCGATTCCGGGCCGGCGC
GGGTTCCTGTCCTCGTTCCATTACGTCACGCTGGTTGGCGGGCACGTGCTGGCGCAGACCACCTTGCTGGTGATGCTGCA
TTTCTTCGATGCCTCGCAGGTCTCCGAATGGGGCTGGCGCGTGGCGTTCGGGCTGGGCGGCATCGGTGCGTTGGTGGTGT
TCTGGCTGCGCCAGACCATGGACGAGTCGCTGAGCTCCGAATCCATCGCCGATTCGCGCACCGGCAAGGCCAAGGCGTCG
GGCTCGATGCGCGAGCTGTTCGTCAACCAGTGGCGCCCGCTGCTGCTGTGCTTCCTGATCACCGCCGGCGGCACGATCGC
GTTCTACACCTATTCGGTCACCGGGCCGAAGATGATCCAGACCGCGTTCGCCGGCGGCGACGTGATGGCCGGCACCATCA
TCAACCTGGTCGCGCTGACCGTGCTGATGCTGATGCAACCGGTCGGCGGCTGGCTGTCGGACATCGTCGGGCGCAAGACC
TTGCTGGTGTTCTTCGGCATCGGCGGCGTGCTCTACACCTGGTTCCTGGTGATGGAACTGCCCAAGCAGACCGACTGGCT
GACCGCGTTCGTGATTCTCACTGTGGGCTTTGTGATCCTGACCGGCTACACCTCGATCAATGCAGTGGTGAAGGCGGAAT
TGTTCCCTACGCATGTGCGCGCGCTGGGCGTCGGTCTTGGCTACGCGTTGGCGAATTCGGCGTTCGGCGGCACCGCGCCG
TTGCTGTATCAGGCCTCGCTGAAGACCAACCAAGTGACCGAGTTCGTGATCTATGCGACGGCGGTGATTGCGGTGTCGTT
GGTGGTCTACATGTTCTTCCTGACCAACAAGGGCAGCAACTGGCTGGACGACGAAACGGCGATGCATCAGCGCAAGCGGT
GA

Upstream 100 bases:

>100_bases
CGGCCGTTGCCAGGCCTGTATATGCGCCGAAATGCTTACATGCGTCACACAAGCGCGCACCTATACTCCGGGGCTGTCCC
ACCGAAGAGGTTCTGACCGC

Downstream 100 bases:

>100_bases
TGAGTGCGCGCGGGCTGGCTGTTTCCTTCTCCCTGCGGGAGAAGGTGCCCGAAGGGCGGATGAGGGTACGAGCGAAGCCT
CGTGTAGTTGGAACGACACA

Product: dicarboxylate transport protein

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSAPPSSPIAAGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKEDKNATMFVWAIFAMTFLMRPI
GAWYFGRFADRYGRRLALTISVSVMALCSFVIAITPTVATIGIAAPIILLVARLLQGFATGGEYGTSATYMSEAAIPGRR
GFLSSFHYVTLVGGHVLAQTTLLVMLHFFDASQVSEWGWRVAFGLGGIGALVVFWLRQTMDESLSSESIADSRTGKAKAS
GSMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQTAFAGGDVMAGTIINLVALTVLMLMQPVGGWLSDIVGRKT
LLVFFGIGGVLYTWFLVMELPKQTDWLTAFVILTVGFVILTGYTSINAVVKAELFPTHVRALGVGLGYALANSAFGGTAP
LLYQASLKTNQVTEFVIYATAVIAVSLVVYMFFLTNKGSNWLDDETAMHQRKR

Sequences:

>Translated_453_residues
MSAPPSSPIAAGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKEDKNATMFVWAIFAMTFLMRPI
GAWYFGRFADRYGRRLALTISVSVMALCSFVIAITPTVATIGIAAPIILLVARLLQGFATGGEYGTSATYMSEAAIPGRR
GFLSSFHYVTLVGGHVLAQTTLLVMLHFFDASQVSEWGWRVAFGLGGIGALVVFWLRQTMDESLSSESIADSRTGKAKAS
GSMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQTAFAGGDVMAGTIINLVALTVLMLMQPVGGWLSDIVGRKT
LLVFFGIGGVLYTWFLVMELPKQTDWLTAFVILTVGFVILTGYTSINAVVKAELFPTHVRALGVGLGYALANSAFGGTAP
LLYQASLKTNQVTEFVIYATAVIAVSLVVYMFFLTNKGSNWLDDETAMHQRKR
>Mature_452_residues
SAPPSSPIAAGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKEDKNATMFVWAIFAMTFLMRPIG
AWYFGRFADRYGRRLALTISVSVMALCSFVIAITPTVATIGIAAPIILLVARLLQGFATGGEYGTSATYMSEAAIPGRRG
FLSSFHYVTLVGGHVLAQTTLLVMLHFFDASQVSEWGWRVAFGLGGIGALVVFWLRQTMDESLSSESIADSRTGKAKASG
SMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQTAFAGGDVMAGTIINLVALTVLMLMQPVGGWLSDIVGRKTL
LVFFGIGGVLYTWFLVMELPKQTDWLTAFVILTVGFVILTGYTSINAVVKAELFPTHVRALGVGLGYALANSAFGGTAPL
LYQASLKTNQVTEFVIYATAVIAVSLVVYMFFLTNKGSNWLDDETAMHQRKR

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=436, Percent_Identity=41.0550458715596, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1790550, Length=382, Percent_Identity=27.4869109947644, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788292, Length=395, Percent_Identity=28.8607594936709, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789941, Length=386, Percent_Identity=30.3108808290155, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87082231, Length=221, Percent_Identity=27.6018099547511, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87082404, Length=354, Percent_Identity=25.4237288135593, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49119; Mature: 48988

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SAPPSSPIAAGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKED
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KNATMFVWAIFAMTFLMRPIGAWYFGRFADRYGRRLALTISVSVMALCSFVIAITPTVAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHH
IGIAAPIILLVARLLQGFATGGEYGTSATYMSEAAIPGRRGFLSSFHYVTLVGGHVLAQT
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHCCHHHHHCHHHHHHH
TLLVMLHFFDASQVSEWGWRVAFGLGGIGALVVFWLRQTMDESLSSESIADSRTGKAKAS
HHHHHHHHHCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCC
GSMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQTAFAGGDVMAGTIINLVALT
CHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHH
VLMLMQPVGGWLSDIVGRKTLLVFFGIGGVLYTWFLVMELPKQTDWLTAFVILTVGFVIL
HHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TGYTSINAVVKAELFPTHVRALGVGLGYALANSAFGGTAPLLYQASLKTNQVTEFVIYAT
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHH
AVIAVSLVVYMFFLTNKGSNWLDDETAMHQRKR
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]