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The map label for this gene is tolC [H]

Identifier: 21244188

GI number: 21244188

Start: 4087550

End: 4088932

Strand: Direct

Name: tolC [H]

Synonym: XAC3463

Alternate gene names: 21244188

Gene position: 4087550-4088932 (Clockwise)

Preceding gene: 21244187

Following gene: 21244190

Centisome position: 78.98

GC content: 65.8

Gene sequence:

>1383_bases
ATGATCCGCCGATCCCTCGTTCTGGCCCTGGCCGCCGCCCTGTCCCCCATGGCTGCGCACGCCACCGACCTGCTGCAGGT
CTACGAAATGGCCCGCAACGGCGATCCGCAGCTGGCCGTGGCCGAGTCCACGCGTCTGGTCAATCGGGAAGGTCAGGTGC
AGGCGCGTGCGGCGTTGTTGCCGCAGCTGGACGGTAGCGCCGGCTACACCCAGTCGCATCGCGAGCTGGAGGGCGTCGAT
GGCCGCTCCACGACCAAGCAGCGCCAGTACGCGATTCAGGGCAGCCAGACCATTTTCAACTGGGCGCAGTTTTCCAACCT
GCGTGCGCAGCGCGAAGTGGCCAAGGCTGCGGATTTCACCCTGGCATCGGCCAACAACGACCTGATCACCCGCACCTCGG
CGGCGTATTTCCAGGTGCTGGTGGGGATCGAGTCGCTGGCGGCAGCCGAGACCAACGAAGCGGCCGCAAAGAAGCAGTTC
GACTATGCCGACAAGCGTCTGGAAGTGGGTCTTGCGCCGATCACCGACGTGCACGAAGCCCGCGCCCAGTACGACCAGGC
GCGTGCAGACACCATCACCGCGCGCAATACGCTCAAGGACTACTACCAGGCGCTGACCGAGCTGACCGGCCAGCCCGTGG
TCGGCTTGCGCGCATTGCCGGATGAGTTCCGCCCCGAAGTACCGGCCGCCTACAGCAACGTGGATCAGCTGGTTGCCACC
GCGATCGCCGACAATCCCGCGCTGAAGGCGCAGCAGTTGCAGGTCAGCGCTGCCGAGGCGCAGGTCAGTGCTGCGCGTGC
CGGCCATCTGCCCACGCTGAGCCTGGGTGCCAATGTCGGCCGCAGCAATAGCTGGGGTGGCCAGGGAACGGTCGACGAGG
CGGCGGGCAACTTCACCACCGCAGGCCGCAATATCGATACCGATTCGGTCGGCATCACGCTGACGATTCCGATCTTTGCC
GGCGGTGCGACCCAGTCGGCCGTGCGCCAGGCATTGTCGCAACGCGACATCCAGCAGGACACCTACGAGCAGCAGAAGCG
CGCGCTGGATCGCAACACCCGCAATGCCTACCAGACCGTCGTTGCCGGCATCAGCGAAGTGGAAGCCCGCCGTCTGGCCG
TGGTGTCGGCGCAAGCCGCCTACGACGCCTCGCAGGTGGGCCTGGAAGTCGGCACGCGCACCGTGCTGGACGTGGTGCAG
AACCAGCGCACGCTGTTCCAGGCGCAGCTGAACTACGCACAGTCGCGTTACAACTTCCTGCAGAACCGCCTGCTGCTGGG
CCAGGCCATCGGCAAGCTCGACATCACCGACCTGCAGGATGTGAATCGCCTGCTGTCGCAGGACGCCGAATCCAAGCTGC
AGGGCAGTGGTTCGTTGCAGTAA

Upstream 100 bases:

>100_bases
CCCCGCGCATCGAATCGTTGTTCGAAACCGACCTCGCCTATCTCCAGGGCGCCGCACCGACGCCCCGATTCCAATTCTGA
TTCCCAAGGAAGCTTCCCCG

Downstream 100 bases:

>100_bases
GACCCACGGGATGCACGAGAAAGCGGGCCCAGGCCCGCTTTTTTTGTGCGCGATGCAACTGCGTGCGCACCTGTTGTGAG
GTCTGTTGCAGGTGCGGTGA

Product: TolC protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MIRRSLVLALAAALSPMAAHATDLLQVYEMARNGDPQLAVAESTRLVNREGQVQARAALLPQLDGSAGYTQSHRELEGVD
GRSTTKQRQYAIQGSQTIFNWAQFSNLRAQREVAKAADFTLASANNDLITRTSAAYFQVLVGIESLAAAETNEAAAKKQF
DYADKRLEVGLAPITDVHEARAQYDQARADTITARNTLKDYYQALTELTGQPVVGLRALPDEFRPEVPAAYSNVDQLVAT
AIADNPALKAQQLQVSAAEAQVSAARAGHLPTLSLGANVGRSNSWGGQGTVDEAAGNFTTAGRNIDTDSVGITLTIPIFA
GGATQSAVRQALSQRDIQQDTYEQQKRALDRNTRNAYQTVVAGISEVEARRLAVVSAQAAYDASQVGLEVGTRTVLDVVQ
NQRTLFQAQLNYAQSRYNFLQNRLLLGQAIGKLDITDLQDVNRLLSQDAESKLQGSGSLQ

Sequences:

>Translated_460_residues
MIRRSLVLALAAALSPMAAHATDLLQVYEMARNGDPQLAVAESTRLVNREGQVQARAALLPQLDGSAGYTQSHRELEGVD
GRSTTKQRQYAIQGSQTIFNWAQFSNLRAQREVAKAADFTLASANNDLITRTSAAYFQVLVGIESLAAAETNEAAAKKQF
DYADKRLEVGLAPITDVHEARAQYDQARADTITARNTLKDYYQALTELTGQPVVGLRALPDEFRPEVPAAYSNVDQLVAT
AIADNPALKAQQLQVSAAEAQVSAARAGHLPTLSLGANVGRSNSWGGQGTVDEAAGNFTTAGRNIDTDSVGITLTIPIFA
GGATQSAVRQALSQRDIQQDTYEQQKRALDRNTRNAYQTVVAGISEVEARRLAVVSAQAAYDASQVGLEVGTRTVLDVVQ
NQRTLFQAQLNYAQSRYNFLQNRLLLGQAIGKLDITDLQDVNRLLSQDAESKLQGSGSLQ
>Mature_460_residues
MIRRSLVLALAAALSPMAAHATDLLQVYEMARNGDPQLAVAESTRLVNREGQVQARAALLPQLDGSAGYTQSHRELEGVD
GRSTTKQRQYAIQGSQTIFNWAQFSNLRAQREVAKAADFTLASANNDLITRTSAAYFQVLVGIESLAAAETNEAAAKKQF
DYADKRLEVGLAPITDVHEARAQYDQARADTITARNTLKDYYQALTELTGQPVVGLRALPDEFRPEVPAAYSNVDQLVAT
AIADNPALKAQQLQVSAAEAQVSAARAGHLPTLSLGANVGRSNSWGGQGTVDEAAGNFTTAGRNIDTDSVGITLTIPIFA
GGATQSAVRQALSQRDIQQDTYEQQKRALDRNTRNAYQTVVAGISEVEARRLAVVSAQAAYDASQVGLEVGTRTVLDVVQ
NQRTLFQAQLNYAQSRYNFLQNRLLLGQAIGKLDITDLQDVNRLLSQDAESKLQGSGSLQ

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=432, Percent_Identity=34.9537037037037, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49605; Mature: 49605

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRSLVLALAAALSPMAAHATDLLQVYEMARNGDPQLAVAESTRLVNREGQVQARAALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCHHHHHHHC
PQLDGSAGYTQSHRELEGVDGRSTTKQRQYAIQGSQTIFNWAQFSNLRAQREVAKAADFT
CCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCE
LASANNDLITRTSAAYFQVLVGIESLAAAETNEAAAKKQFDYADKRLEVGLAPITDVHEA
EECCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RAQYDQARADTITARNTLKDYYQALTELTGQPVVGLRALPDEFRPEVPAAYSNVDQLVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHH
AIADNPALKAQQLQVSAAEAQVSAARAGHLPTLSLGANVGRSNSWGGQGTVDEAAGNFTT
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHCCCCEE
AGRNIDTDSVGITLTIPIFAGGATQSAVRQALSQRDIQQDTYEQQKRALDRNTRNAYQTV
CCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VAGISEVEARRLAVVSAQAAYDASQVGLEVGTRTVLDVVQNQRTLFQAQLNYAQSRYNFL
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNRLLLGQAIGKLDITDLQDVNRLLSQDAESKLQGSGSLQ
HHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHCCCCCCC
>Mature Secondary Structure
MIRRSLVLALAAALSPMAAHATDLLQVYEMARNGDPQLAVAESTRLVNREGQVQARAALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCHHHHHHHC
PQLDGSAGYTQSHRELEGVDGRSTTKQRQYAIQGSQTIFNWAQFSNLRAQREVAKAADFT
CCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCE
LASANNDLITRTSAAYFQVLVGIESLAAAETNEAAAKKQFDYADKRLEVGLAPITDVHEA
EECCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RAQYDQARADTITARNTLKDYYQALTELTGQPVVGLRALPDEFRPEVPAAYSNVDQLVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHH
AIADNPALKAQQLQVSAAEAQVSAARAGHLPTLSLGANVGRSNSWGGQGTVDEAAGNFTT
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHCCCCEE
AGRNIDTDSVGITLTIPIFAGGATQSAVRQALSQRDIQQDTYEQQKRALDRNTRNAYQTV
CCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VAGISEVEARRLAVVSAQAAYDASQVGLEVGTRTVLDVVQNQRTLFQAQLNYAQSRYNFL
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNRLLLGQAIGKLDITDLQDVNRLLSQDAESKLQGSGSLQ
HHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6312426; 2216730; 9278503; 6303857; 9298646; 9629924; 10879525 [H]