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The map label for this gene is htrB [H]

Identifier: 21244190

GI number: 21244190

Start: 4090809

End: 4091729

Strand: Direct

Name: htrB [H]

Synonym: XAC3465

Alternate gene names: 21244190

Gene position: 4090809-4091729 (Clockwise)

Preceding gene: 21244188

Following gene: 77748717

Centisome position: 79.04

GC content: 65.8

Gene sequence:

>921_bases
ATGTCCGAGTCCGCCAACGTCGCCGTCCGCCCTTCCCTGCGCAACCCCAAGCATTGGCCGATGTATCTGGGCCTGGCGGT
GATGGTGGTGGCGGGGCGCCTGCCCTGGACGCTGCAACGTGCAGTAGGCCGCGGCGTGGGCTGGATTGCGATGCGCCTGG
CGGGCACACGTCGGCGCGCGGCCGAGGTCAACCTCAAACTGTGTTTCCCCGAGCAGGACGATGCCTGGCGAGCGCGGCTG
TTGCGCGACAGTTTCGACGCGCTCGGCGTGGGCCTGTTCGAGTTCGCGCGCGCCTGGTGGGGCAGCATCGGTGCCATTCG
ACCTGGCGTGCAGATCGAGGGCCTGGAGCATCTGCGGCAACTGCAACAGCAAAAGCGCGGCGTGTTGCTGGTGTCCGGCC
ACTTCATGACGCTGGAAATGTGCGGGCGCCTGCTTTGCGACCACGTGCCGCTGGCCGGCATGTACCGCCGGCATCGCAAC
CCGGTGTTCGAGTGGGCGGTCAAGCGTGGGCGCTTGCGCTATGCCACGCACATGTTCGCCAACGAAGACCTGCGCGCCAC
CATCAAGCATCTCAAGCGCGGCGGTTTTCTGTGGTATGCGCCCGACCAGGACATGCGCGGCAAGGACACCGTGTTCGTGC
CGTTCTTCGGACATCCGGCCTCCACCATCACCGCCACCCATCAGCTGGCGCGGTTGACCGGCTGCGCGGTAGTGCCCTAC
TTCCATCGTCGCGAAGGTGGCCGTTACATCCTCAAGATCGCGCCACCGTTGGCCGACATTCCGTCCGATGACGTCATCGC
CGATACCACGCAGGTCAATGCCGCGATCGAAGACATGGTGCGCGAAGCGCCGGACCAGTACCTGTGGATCCATCGGCGCT
TCAAGCGCCAGCCGGGTGGACGTAGCGCGTTCTATCGCTGA

Upstream 100 bases:

>100_bases
CTTCCGGAGGGTTGGCCGCCAGCGGCGCGGAGCGGGCGCCGCGCCGCTGGCGGCCAACGCATCTCATCTCAACGTGCATA
ACACGCTCTAGAATTGGCGC

Downstream 100 bases:

>100_bases
CGCCGACCCGGCACGCCTGCAAAGCAGGTGTGGGCGTTGCCGCTGCAAGCTCGCACATCGACGCGGTGCCGATCACCCGT
ACGCACCGCGTTACTGCGGC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSESANVAVRPSLRNPKHWPMYLGLAVMVVAGRLPWTLQRAVGRGVGWIAMRLAGTRRRAAEVNLKLCFPEQDDAWRARL
LRDSFDALGVGLFEFARAWWGSIGAIRPGVQIEGLEHLRQLQQQKRGVLLVSGHFMTLEMCGRLLCDHVPLAGMYRRHRN
PVFEWAVKRGRLRYATHMFANEDLRATIKHLKRGGFLWYAPDQDMRGKDTVFVPFFGHPASTITATHQLARLTGCAVVPY
FHRREGGRYILKIAPPLADIPSDDVIADTTQVNAAIEDMVREAPDQYLWIHRRFKRQPGGRSAFYR

Sequences:

>Translated_306_residues
MSESANVAVRPSLRNPKHWPMYLGLAVMVVAGRLPWTLQRAVGRGVGWIAMRLAGTRRRAAEVNLKLCFPEQDDAWRARL
LRDSFDALGVGLFEFARAWWGSIGAIRPGVQIEGLEHLRQLQQQKRGVLLVSGHFMTLEMCGRLLCDHVPLAGMYRRHRN
PVFEWAVKRGRLRYATHMFANEDLRATIKHLKRGGFLWYAPDQDMRGKDTVFVPFFGHPASTITATHQLARLTGCAVVPY
FHRREGGRYILKIAPPLADIPSDDVIADTTQVNAAIEDMVREAPDQYLWIHRRFKRQPGGRSAFYR
>Mature_305_residues
SESANVAVRPSLRNPKHWPMYLGLAVMVVAGRLPWTLQRAVGRGVGWIAMRLAGTRRRAAEVNLKLCFPEQDDAWRARLL
RDSFDALGVGLFEFARAWWGSIGAIRPGVQIEGLEHLRQLQQQKRGVLLVSGHFMTLEMCGRLLCDHVPLAGMYRRHRNP
VFEWAVKRGRLRYATHMFANEDLRATIKHLKRGGFLWYAPDQDMRGKDTVFVPFFGHPASTITATHQLARLTGCAVVPYF
HRREGGRYILKIAPPLADIPSDDVIADTTQVNAAIEDMVREAPDQYLWIHRRFKRQPGGRSAFYR

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=297, Percent_Identity=38.047138047138, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082094, Length=294, Percent_Identity=36.0544217687075, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1788161, Length=300, Percent_Identity=27.6666666666667, Blast_Score=124, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 34907; Mature: 34776

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESANVAVRPSLRNPKHWPMYLGLAVMVVAGRLPWTLQRAVGRGVGWIAMRLAGTRRRA
CCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCHHHH
AEVNLKLCFPEQDDAWRARLLRDSFDALGVGLFEFARAWWGSIGAIRPGVQIEGLEHLRQ
HEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHH
LQQQKRGVLLVSGHFMTLEMCGRLLCDHVPLAGMYRRHRNPVFEWAVKRGRLRYATHMFA
HHHHHCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHC
NEDLRATIKHLKRGGFLWYAPDQDMRGKDTVFVPFFGHPASTITATHQLARLTGCAVVPY
CCHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEECCH
FHRREGGRYILKIAPPLADIPSDDVIADTTQVNAAIEDMVREAPDQYLWIHRRFKRQPGG
HHHCCCCEEEEEECCCCCCCCCCCCEECHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
RSAFYR
CCCCCC
>Mature Secondary Structure 
SESANVAVRPSLRNPKHWPMYLGLAVMVVAGRLPWTLQRAVGRGVGWIAMRLAGTRRRA
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCHHHH
AEVNLKLCFPEQDDAWRARLLRDSFDALGVGLFEFARAWWGSIGAIRPGVQIEGLEHLRQ
HEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHH
LQQQKRGVLLVSGHFMTLEMCGRLLCDHVPLAGMYRRHRNPVFEWAVKRGRLRYATHMFA
HHHHHCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHC
NEDLRATIKHLKRGGFLWYAPDQDMRGKDTVFVPFFGHPASTITATHQLARLTGCAVVPY
CCHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEECCH
FHRREGGRYILKIAPPLADIPSDDVIADTTQVNAAIEDMVREAPDQYLWIHRRFKRQPGG
HHHCCCCEEEEEECCCCCCCCCCCCEECHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
RSAFYR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]