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The map label for this gene is leuA

Identifier: 21244180

GI number: 21244180

Start: 4076380

End: 4077942

Strand: Direct

Name: leuA

Synonym: XAC3455

Alternate gene names: 21244180

Gene position: 4076380-4077942 (Clockwise)

Preceding gene: 21244179

Following gene: 21244184

Centisome position: 78.76

GC content: 65.13

Gene sequence:

>1563_bases
GTGAACACGACCGTATCCAACCAGACCCCGCGTATCCGAATTTTCGACACCACCCTGCGCGACGGCGAGCAATCCCCCGG
CTGCAGCATGACGCCCCAGCAGAAGCTGGTCATGGCGCGCGCGCTGGACGAACTCGGCGTGGACATCATCGAAACCGGTT
TTCCTGCCAGCTCGCATTCGGACCGCGAAGCGGTGGCGATGATGGGCCGGGAACTGCGCCGCCCCACCCTGGCGGTGTTG
TCGCGCTGCCTGCAGGCCGATATCGAAACCTCGGCCAAGGCGCTGGAGACAGCCGCAAACCCGCGCCTGCATGTGTTCCT
GTCCACCAGCCCGCTGCACCGCGAGCACAAGCTGCGCATGAGCCGCGAGCAGGTGCTGGAATCGGTGCACCGACACGTGA
CGCTGGCGCGCGGCTATGTCGACGATGTGGAATTTTCTGCCGAGGATGCAACCCGCACCGAAGAAGATTTCCTGGCCGAG
GTCACCCGCGTGGCGATCGCCGCCGGCGCCACCACGATCAATTTGCCCGACACGGTCGGCTTCACCACGCCGGAAGAAAT
CCGTGGCATGTTCTCGCGCCTGATCGCCAGCGTGGAAGGTGCCGACAAGGTCATCTTCAGCGCGCACTGCCACAACGATC
TGGGCCTGGCGGTGGCCAACTCGCTGGCGGCCATCGAAGGCGGTGCGCGCCAGGTGGAATGCACCATCAATGGCATCGGT
GAGCGCGCGGGCAATTGCGCGCTGGAAGAAATCACCATGGCGCTGAAGGTGCGCGGTGCCTTCTACAACATCGATTCGGC
GATCAATACGCCGCGTATCGTGTCTACCTCGCAATTGTTGCAACGCCTGGTCGGCATGCCGGTGCAGCGCAACAAGGCAG
TCGTGGGCGGCAACGCCTTCGCGCACGAATCGGGCATTCATCAGCACGGCATGCTGCGCCATCGCGGCACCTACGAAATC
ATGCGCCCGGAAGATGTAGGCTGGGAATCCTCGCAAATGGTACTGGGCCGCCACAGCGGCCGCGCCGCGGTCGAGCAGCG
GCTGCGCGCGCTGGGTTATCTGCTGGAGGAGGAAGAGGTCAAGCTGGTGTTCGAACAGTTCAAGGCGCTGTGCGAGAAGC
AGCGCGTGGTGACCGATGCCGATCTGCAGGCATTGATGCAGGACGCCACGGTGCAGGAAGGCTATCGCCTGGCGTCGATG
ACCATCAGCGACGTGGGCAGCCGCGCCAACGCGTTGGTGGAGTTGTCCGACCCAGAAGGCAACCGTGTGGCCGAAACCGC
GCAGGGCAACGGGCCGGTGGATGCGCTCTTCGGCGCACTGGCCTCGGCCACCGGCGTGAAGCTGGAGCTGGACAGCTACC
AGGTGCACAGCGTGGGCATCGGTGCGGACGCGCGCGGCGAAGCCAGCCTGAGCGTGCGCCACGACGGCGTGGAATACGAA
GGCACCGGCACCAGCAAGGACATCATCGAAGCCAGCGCGCTTGCGTGGCTGGATGTGGCCAACCGCTTGTTGCGTCAGCG
CGAGCGTGGCGTGGTCGCCGGCAAGACCGCTGCGGTGGCGTAA

Upstream 100 bases:

>100_bases
AACGCAGCGCCAATCCGCCACCCGCCACACACGTTCTTTCCCGCCCATCCGCAGTCGCTTCAACGCCTGTCACCGCCATC
GCCGTAGAGGAGTCTTCCTG

Downstream 100 bases:

>100_bases
CCCTGCTCATTTCATTGGGTAGATGACGCAAGCCTTACGGCCAGCAGAGTGATCTGCTGGCCGTTCCGGTTTTTGCGTGC
GAACTTTTTACAAGTGTGTC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MNTTVSNQTPRIRIFDTTLRDGEQSPGCSMTPQQKLVMARALDELGVDIIETGFPASSHSDREAVAMMGRELRRPTLAVL
SRCLQADIETSAKALETAANPRLHVFLSTSPLHREHKLRMSREQVLESVHRHVTLARGYVDDVEFSAEDATRTEEDFLAE
VTRVAIAAGATTINLPDTVGFTTPEEIRGMFSRLIASVEGADKVIFSAHCHNDLGLAVANSLAAIEGGARQVECTINGIG
ERAGNCALEEITMALKVRGAFYNIDSAINTPRIVSTSQLLQRLVGMPVQRNKAVVGGNAFAHESGIHQHGMLRHRGTYEI
MRPEDVGWESSQMVLGRHSGRAAVEQRLRALGYLLEEEEVKLVFEQFKALCEKQRVVTDADLQALMQDATVQEGYRLASM
TISDVGSRANALVELSDPEGNRVAETAQGNGPVDALFGALASATGVKLELDSYQVHSVGIGADARGEASLSVRHDGVEYE
GTGTSKDIIEASALAWLDVANRLLRQRERGVVAGKTAAVA

Sequences:

>Translated_520_residues
MNTTVSNQTPRIRIFDTTLRDGEQSPGCSMTPQQKLVMARALDELGVDIIETGFPASSHSDREAVAMMGRELRRPTLAVL
SRCLQADIETSAKALETAANPRLHVFLSTSPLHREHKLRMSREQVLESVHRHVTLARGYVDDVEFSAEDATRTEEDFLAE
VTRVAIAAGATTINLPDTVGFTTPEEIRGMFSRLIASVEGADKVIFSAHCHNDLGLAVANSLAAIEGGARQVECTINGIG
ERAGNCALEEITMALKVRGAFYNIDSAINTPRIVSTSQLLQRLVGMPVQRNKAVVGGNAFAHESGIHQHGMLRHRGTYEI
MRPEDVGWESSQMVLGRHSGRAAVEQRLRALGYLLEEEEVKLVFEQFKALCEKQRVVTDADLQALMQDATVQEGYRLASM
TISDVGSRANALVELSDPEGNRVAETAQGNGPVDALFGALASATGVKLELDSYQVHSVGIGADARGEASLSVRHDGVEYE
GTGTSKDIIEASALAWLDVANRLLRQRERGVVAGKTAAVA
>Mature_520_residues
MNTTVSNQTPRIRIFDTTLRDGEQSPGCSMTPQQKLVMARALDELGVDIIETGFPASSHSDREAVAMMGRELRRPTLAVL
SRCLQADIETSAKALETAANPRLHVFLSTSPLHREHKLRMSREQVLESVHRHVTLARGYVDDVEFSAEDATRTEEDFLAE
VTRVAIAAGATTINLPDTVGFTTPEEIRGMFSRLIASVEGADKVIFSAHCHNDLGLAVANSLAAIEGGARQVECTINGIG
ERAGNCALEEITMALKVRGAFYNIDSAINTPRIVSTSQLLQRLVGMPVQRNKAVVGGNAFAHESGIHQHGMLRHRGTYEI
MRPEDVGWESSQMVLGRHSGRAAVEQRLRALGYLLEEEEVKLVFEQFKALCEKQRVVTDADLQALMQDATVQEGYRLASM
TISDVGSRANALVELSDPEGNRVAETAQGNGPVDALFGALASATGVKLELDSYQVHSVGIGADARGEASLSVRHDGVEYE
GTGTSKDIIEASALAWLDVANRLLRQRERGVVAGKTAAVA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=295, Percent_Identity=23.3898305084746, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1786261, Length=504, Percent_Identity=45.4365079365079, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6320071, Length=380, Percent_Identity=28.6842105263158, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6320019, Length=380, Percent_Identity=28.4210526315789, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6324682, Length=560, Percent_Identity=24.4642857142857, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=587, Percent_Identity=24.3611584327087, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24582381, Length=295, Percent_Identity=24.7457627118644, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_XANAC (P58900)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643762.1
- ProteinModelPortal:   P58900
- SMR:   P58900
- GeneID:   1157526
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3455
- NMPDR:   fig|190486.1.peg.3406
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   XAXO190486:XAC3455-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56289; Mature: 56289

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTVSNQTPRIRIFDTTLRDGEQSPGCSMTPQQKLVMARALDELGVDIIETGFPASSHS
CCCCCCCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
DREAVAMMGRELRRPTLAVLSRCLQADIETSAKALETAANPRLHVFLSTSPLHREHKLRM
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
SREQVLESVHRHVTLARGYVDDVEFSAEDATRTEEDFLAEVTRVAIAAGATTINLPDTVG
HHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCC
FTTPEEIRGMFSRLIASVEGADKVIFSAHCHNDLGLAVANSLAAIEGGARQVECTINGIG
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCC
ERAGNCALEEITMALKVRGAFYNIDSAINTPRIVSTSQLLQRLVGMPVQRNKAVVGGNAF
CCCCCHHHHHHHHHHHCCCCEEECCHHCCCCCEECHHHHHHHHHCCCCCCCCEEECCCCH
AHESGIHQHGMLRHRGTYEIMRPEDVGWESSQMVLGRHSGRAAVEQRLRALGYLLEEEEV
HHCCCCCCCCCHHCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
KLVFEQFKALCEKQRVVTDADLQALMQDATVQEGYRLASMTISDVGSRANALVELSDPEG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCEEEEECCCCC
NRVAETAQGNGPVDALFGALASATGVKLELDSYQVHSVGIGADARGEASLSVRHDGVEYE
CCEECCCCCCCCHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCCCCCEEEEEECCEEEC
GTGTSKDIIEASALAWLDVANRLLRQRERGVVAGKTAAVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHCCC
>Mature Secondary Structure
MNTTVSNQTPRIRIFDTTLRDGEQSPGCSMTPQQKLVMARALDELGVDIIETGFPASSHS
CCCCCCCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
DREAVAMMGRELRRPTLAVLSRCLQADIETSAKALETAANPRLHVFLSTSPLHREHKLRM
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
SREQVLESVHRHVTLARGYVDDVEFSAEDATRTEEDFLAEVTRVAIAAGATTINLPDTVG
HHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCC
FTTPEEIRGMFSRLIASVEGADKVIFSAHCHNDLGLAVANSLAAIEGGARQVECTINGIG
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCC
ERAGNCALEEITMALKVRGAFYNIDSAINTPRIVSTSQLLQRLVGMPVQRNKAVVGGNAF
CCCCCHHHHHHHHHHHCCCCEEECCHHCCCCCEECHHHHHHHHHCCCCCCCCEEECCCCH
AHESGIHQHGMLRHRGTYEIMRPEDVGWESSQMVLGRHSGRAAVEQRLRALGYLLEEEEV
HHCCCCCCCCCHHCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
KLVFEQFKALCEKQRVVTDADLQALMQDATVQEGYRLASMTISDVGSRANALVELSDPEG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCEEEEECCCCC
NRVAETAQGNGPVDALFGALASATGVKLELDSYQVHSVGIGADARGEASLSVRHDGVEYE
CCEECCCCCCCCHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCCCCCEEEEEECCEEEC
GTGTSKDIIEASALAWLDVANRLLRQRERGVVAGKTAAVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217