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The map label for this gene is cysI

Identifier: 21244056

GI number: 21244056

Start: 3924863

End: 3926569

Strand: Direct

Name: cysI

Synonym: XAC3331

Alternate gene names: 21244056

Gene position: 3924863-3926569 (Clockwise)

Preceding gene: 21244055

Following gene: 21244057

Centisome position: 75.83

GC content: 65.61

Gene sequence:

>1707_bases
ATGAGCCACTCCGTCGAAGACATCAAATCCGAGAGCCGCCGTCTGCGTGGGTCGCTGGAACAGAGCCTGGCCGATGCGGT
GACCGGTGCGTTGCGTGAGGACGATCAGACCTTGATCAAGTACCACGGCAGCTACCAGCAGGACGACCGCGATATTCGCG
ACGAGCGGCGGCGGCAGAAGCTGGAGCCGGCGTACCAGTTCATGATCCGCACGCGCACGCCGGGCGGGGTGATCACGCCC
ACGCAATGGCTGGCACTGGATGGCATCGCCACCCGCTACGCCAATCATTCGCTACGCATCACCACGCGCCAGGCATTCCA
GTTTCATGGCGTGATCAAGCGCGAATTGAAGGCCACCATGCAGGCCATCAATGCCACTCTGATCGATACGCTGGCCGCCT
GCGGCGATGTGAATCGCAACGTGCAGGTGGCCGCGAATCCGCTGCTGTCGCAGGCGCATGCCACGCTGTATGCCGATGCC
GCGCGCGTTTCCGAACACCTGTTGCCCAACACGCGGGCGTACTACGAAATCTGGCTGGACGAAGAGCGCGTGTCCGGCTC
CGGCAGCGAGGACGAGCCGATCTATGGCGAGCGCTATCTACCGCGCAAATTCAAGATCGGTTTCGCCGCACCGCCGCTCA
ACGATGTGGACGTGTTCGCCAACGATCTGGGCTTTATCGCTATCCTGCGCGATGGGCAGTTGCTGGGCTACAACGTCAGC
ATCGGTGGCGGCATGGGCGCCTCGCACGGCGATGCCGAGACCTGGCCGCGCGTGGCCAACGTGATCGGCTTCGTGACCCG
CGAACAGTTGCTGGATATCGCCACTGCGGTGGTCACCACGCAACGCGACTTCGGCAACCGTGCGGTGCGCAAGCGCGCAC
GCTTCAAGTACACCATCGACGACCACGGGCTGGACACCATCGTGGCCGAGATCGCACGCCGCGCCGGCTTCGCACTGCAA
CCGGCGCAGCCGTTCGCGTTCGACCACAACGGCGACCGCTATGGCTGGGTCGAAGGCGAAGACGGGCTATGGCATCTGAC
CCTGTCGCTGCCGGCCGGCCGCATTGCCGACACCGATACCGCAGCGCATCTGAGTGGGTTGCGCGCAATCGCGCAGTTGA
ACGTTGGCGAGTTCCGCATGACGCCCAACCAGAACCTGGTGATCGCCGGCGTACTGGCCAGCGAGCGCGCACGCGTCGAT
GCGCTGGTGGCGCACTACGGGCTGGACGCCGGCAACCAGTCCGCCACTGCCTTGGCGCGCGGCGCAATGGCCTGCGTGGC
ACTGCCGACCTGTGGCCTGGCAATGGCCGAGGCCGAGCGCTATCTGCCCGATTTCGGCGCGGCGCTGCAGCCGCTGCTGC
AACAGCACGGCCTGGCCGAGACGCCGATCGTGCTGCGCTTGTCCGGCTGCCCGAACGGCTGCTCGCGCCCGTATCTTGCC
GAGATTGCGCTGGTCGGCAAGGCGCCCGGGCGCTACAACCTGATGCTGGGTGGCGACCGTCGCGGGCAGCGGCTCAATAC
GCTGTATCGCGAAAACATCACCGAGCCGGAGATCCTGGCCGCGTTGGAACCGCTGTTGGCGCGCTATGCCGCCGAACGCG
ACCACGCCAACGATGAAGGCTTCGGCGATTTCCTGCATCGCGCCGGGGTGATCGCATTGCCGCCCTACCCCACGCACCGT
CGCCTCGACCTGGAATTGCTCGCATGA

Upstream 100 bases:

>100_bases
ATCGCGAGTGGGGAATCGGGAATGGGAAGAGCCCATGTCCGAGTTCTTCCGACCAACTGCGCCGCTTTTCACGATTCCCC
ATTCCCGATTCTCGATTCTC

Downstream 100 bases:

>100_bases
CCGCGCTGCCTGCTGCATCGATCACTTCTTCCGCGTTCGACGATCTGGACGCGCTCAATGCGCAGCTGGAAGGGCTGCGT
GCCGATCAACGCGTGGCCTG

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MSHSVEDIKSESRRLRGSLEQSLADAVTGALREDDQTLIKYHGSYQQDDRDIRDERRRQKLEPAYQFMIRTRTPGGVITP
TQWLALDGIATRYANHSLRITTRQAFQFHGVIKRELKATMQAINATLIDTLAACGDVNRNVQVAANPLLSQAHATLYADA
ARVSEHLLPNTRAYYEIWLDEERVSGSGSEDEPIYGERYLPRKFKIGFAAPPLNDVDVFANDLGFIAILRDGQLLGYNVS
IGGGMGASHGDAETWPRVANVIGFVTREQLLDIATAVVTTQRDFGNRAVRKRARFKYTIDDHGLDTIVAEIARRAGFALQ
PAQPFAFDHNGDRYGWVEGEDGLWHLTLSLPAGRIADTDTAAHLSGLRAIAQLNVGEFRMTPNQNLVIAGVLASERARVD
ALVAHYGLDAGNQSATALARGAMACVALPTCGLAMAEAERYLPDFGAALQPLLQQHGLAETPIVLRLSGCPNGCSRPYLA
EIALVGKAPGRYNLMLGGDRRGQRLNTLYRENITEPEILAALEPLLARYAAERDHANDEGFGDFLHRAGVIALPPYPTHR
RLDLELLA

Sequences:

>Translated_568_residues
MSHSVEDIKSESRRLRGSLEQSLADAVTGALREDDQTLIKYHGSYQQDDRDIRDERRRQKLEPAYQFMIRTRTPGGVITP
TQWLALDGIATRYANHSLRITTRQAFQFHGVIKRELKATMQAINATLIDTLAACGDVNRNVQVAANPLLSQAHATLYADA
ARVSEHLLPNTRAYYEIWLDEERVSGSGSEDEPIYGERYLPRKFKIGFAAPPLNDVDVFANDLGFIAILRDGQLLGYNVS
IGGGMGASHGDAETWPRVANVIGFVTREQLLDIATAVVTTQRDFGNRAVRKRARFKYTIDDHGLDTIVAEIARRAGFALQ
PAQPFAFDHNGDRYGWVEGEDGLWHLTLSLPAGRIADTDTAAHLSGLRAIAQLNVGEFRMTPNQNLVIAGVLASERARVD
ALVAHYGLDAGNQSATALARGAMACVALPTCGLAMAEAERYLPDFGAALQPLLQQHGLAETPIVLRLSGCPNGCSRPYLA
EIALVGKAPGRYNLMLGGDRRGQRLNTLYRENITEPEILAALEPLLARYAAERDHANDEGFGDFLHRAGVIALPPYPTHR
RLDLELLA
>Mature_567_residues
SHSVEDIKSESRRLRGSLEQSLADAVTGALREDDQTLIKYHGSYQQDDRDIRDERRRQKLEPAYQFMIRTRTPGGVITPT
QWLALDGIATRYANHSLRITTRQAFQFHGVIKRELKATMQAINATLIDTLAACGDVNRNVQVAANPLLSQAHATLYADAA
RVSEHLLPNTRAYYEIWLDEERVSGSGSEDEPIYGERYLPRKFKIGFAAPPLNDVDVFANDLGFIAILRDGQLLGYNVSI
GGGMGASHGDAETWPRVANVIGFVTREQLLDIATAVVTTQRDFGNRAVRKRARFKYTIDDHGLDTIVAEIARRAGFALQP
AQPFAFDHNGDRYGWVEGEDGLWHLTLSLPAGRIADTDTAAHLSGLRAIAQLNVGEFRMTPNQNLVIAGVLASERARVDA
LVAHYGLDAGNQSATALARGAMACVALPTCGLAMAEAERYLPDFGAALQPLLQQHGLAETPIVLRLSGCPNGCSRPYLAE
IALVGKAPGRYNLMLGGDRRGQRLNTLYRENITEPEILAALEPLLARYAAERDHANDEGFGDFLHRAGVIALPPYPTHRR
LDLELLA

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=547, Percent_Identity=50.091407678245, Blast_Score=563, Evalue=1e-161,
Organism=Saccharomyces cerevisiae, GI6322597, Length=578, Percent_Identity=45.1557093425605, Blast_Score=522, Evalue=1e-149,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_XANAC (Q8PHC8)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643638.1
- HSSP:   P17846
- ProteinModelPortal:   Q8PHC8
- SMR:   Q8PHC8
- GeneID:   1157402
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3331
- NMPDR:   fig|190486.1.peg.3282
- HOGENOM:   HBG626671
- OMA:   ESERVFP
- ProtClustDB:   PRK13504
- BioCyc:   XAXO190486:XAC3331-MONOMER
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 62418; Mature: 62287

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSVEDIKSESRRLRGSLEQSLADAVTGALREDDQTLIKYHGSYQQDDRDIRDERRRQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHH
LEPAYQFMIRTRTPGGVITPTQWLALDGIATRYANHSLRITTRQAFQFHGVIKRELKATM
CCHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHH
QAINATLIDTLAACGDVNRNVQVAANPLLSQAHATLYADAARVSEHLLPNTRAYYEIWLD
HHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
EERVSGSGSEDEPIYGERYLPRKFKIGFAAPPLNDVDVFANDLGFIAILRDGQLLGYNVS
HHHCCCCCCCCCCCCCCCCCCCEEECEECCCCCCCHHHHHCCCCEEEEEECCEEEEEEEE
IGGGMGASHGDAETWPRVANVIGFVTREQLLDIATAVVTTQRDFGNRAVRKRARFKYTID
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
DHGLDTIVAEIARRAGFALQPAQPFAFDHNGDRYGWVEGEDGLWHLTLSLPAGRIADTDT
CCCHHHHHHHHHHHCCCEECCCCCCEECCCCCEEEEEECCCCEEEEEEECCCCCCCCCHH
AAHLSGLRAIAQLNVGEFRMTPNQNLVIAGVLASERARVDALVAHYGLDAGNQSATALAR
HHHHHHHHHHHHCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHH
GAMACVALPTCGLAMAEAERYLPDFGAALQPLLQQHGLAETPIVLRLSGCPNGCSRPYLA
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEE
EIALVGKAPGRYNLMLGGDRRGQRLNTLYRENITEPEILAALEPLLARYAAERDHANDEG
EEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
FGDFLHRAGVIALPPYPTHRRLDLELLA
HHHHHHHCCEEEECCCCCCCEEEEEEEC
>Mature Secondary Structure 
SHSVEDIKSESRRLRGSLEQSLADAVTGALREDDQTLIKYHGSYQQDDRDIRDERRRQK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHH
LEPAYQFMIRTRTPGGVITPTQWLALDGIATRYANHSLRITTRQAFQFHGVIKRELKATM
CCHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHH
QAINATLIDTLAACGDVNRNVQVAANPLLSQAHATLYADAARVSEHLLPNTRAYYEIWLD
HHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
EERVSGSGSEDEPIYGERYLPRKFKIGFAAPPLNDVDVFANDLGFIAILRDGQLLGYNVS
HHHCCCCCCCCCCCCCCCCCCCEEECEECCCCCCCHHHHHCCCCEEEEEECCEEEEEEEE
IGGGMGASHGDAETWPRVANVIGFVTREQLLDIATAVVTTQRDFGNRAVRKRARFKYTID
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
DHGLDTIVAEIARRAGFALQPAQPFAFDHNGDRYGWVEGEDGLWHLTLSLPAGRIADTDT
CCCHHHHHHHHHHHCCCEECCCCCCEECCCCCEEEEEECCCCEEEEEEECCCCCCCCCHH
AAHLSGLRAIAQLNVGEFRMTPNQNLVIAGVLASERARVDALVAHYGLDAGNQSATALAR
HHHHHHHHHHHHCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHH
GAMACVALPTCGLAMAEAERYLPDFGAALQPLLQQHGLAETPIVLRLSGCPNGCSRPYLA
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEE
EIALVGKAPGRYNLMLGGDRRGQRLNTLYRENITEPEILAALEPLLARYAAERDHANDEG
EEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
FGDFLHRAGVIALPPYPTHRRLDLELLA
HHHHHHHCCEEEECCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217