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The map label for this gene is prfC [H]

Identifier: 21243769

GI number: 21243769

Start: 3559192

End: 3560796

Strand: Direct

Name: prfC [H]

Synonym: XAC3042

Alternate gene names: 21243769

Gene position: 3559192-3560796 (Clockwise)

Preceding gene: 77748691

Following gene: 21243770

Centisome position: 68.77

GC content: 62.68

Gene sequence:

>1605_bases
ATGTCCGAAGTCTCCAACGAAGCCGCGCGCCGCCGCACCTTCGCCATCATTTCCCACCCCGATGCCGGCAAGACCACGCT
GACCGAAAAACTGTTGCTGTTCGGCGGCGCGATCCAGATGGCCGGCTCGGTGAAGGGCCGCAAGGCCGCACGCCATGCCA
CCTCGGACTGGATGGCGCTGGAAAAAGAGCGCGGCATCTCGGTGACCTCCTCGGTCATGCAGTTTCCGTACGAGGGCAAG
ATCGTCAACCTGCTCGACACGCCCGGCCACGCCGACTTCGGCGAGGACACCTATCGCGTGCTCACCGCGGTGGACTCGGC
GCTGATGGTCATCGACGTGGCCAAGGGCGTGGAAGAACGCACCATCAAACTGATGGAAGTCTGCCGGCTGCGCGACACCC
CCATCATGACCTTCATCAACAAGCTCGATCGCGAAGGCAAGAACCCGATCGATCTGTTGGATGAAGTGGAAACCGTGCTC
GGCATCCAGTGCGCGCCGGTCACCTGGCCAATCGGCATGGGCCAGCGCCTCAAGGGCGTGGTGCACCTGATCTCCGGCGA
AGTGCATCTGTACGAACAGGGACGCAATTTCACCCGTCAGGATTCCACCATCTTCCCCTCGCTGGATGCGCCCGGCCTGG
TCGAAAAGATCGGCGAGCAGATGCTGGCCGAGCTGCGCGAGGAGCTGGAACTGGTGCAAGGTGCCAGCAACCCGTTCGAT
CTTGACGCGTACCGCGCGGGCCAACAGACCCCGGTGTTCTTCGGTTCGGGTGTCAACAATTTCGGCGTGCAACCGTTGCT
GGATTTCTTCGTCGAACACGCACCGCCGCCGCAGACGCGTGAGACCACCGGCCGCCGCGTGGAGCCGACCGAAGCCAAAC
TGAGCGGCTTCGTTTTCAAGATCCAGGCCAACATGGATCCGCAGCATCGCGACCGCGTGGCCTTCATGCGCGTTTGCTCT
GGCAAGTTCACTGCCGGCATGAAGACGCTGCATGTGCGCAGCGGCAAGGACGTCAAATTGGCCAACGCACTGACCTTCAT
GGCGTCCGATCGCGAGATCGCCGCAGAAGCGTGGCCGGGCGATGTCATCGGCATCCACAACCACGGCACCATTTCCATCG
GCGACACCTTCACCGAAGGCGAATCGCTGTCGTTCACCGGCATTCCCAACTTCGCGCCGGAACTGTTCCGCCGCGCGCGC
CTACGCGATCCGCTCAAGCTCAAGCAATTGCAGAAGGGTCTTGCGCAGCTGTCCGAAGAAGGCGCCACGCAATTCTTCCG
CCCGTTGATGAGCAACGACCTGATCCTGGGCGCGGTCGGTGTACTGCAGTTCGACGTGGTCGCGTATCGGCTCAAGGACG
AATACGGCGTGGATGCGATCTTCGAGCCGGTGAGCGTCACCACTGCACGCTGGGTGCACTGCGACAACCCCAAGAAGCTG
GAAGAATTCCGCGAGAAAAATGCCGGCAACCTGGGCATCGACGCGGCCGGCGAGCTGGTGTACCTGGCACCGACCCGCGT
GAATCTGCAGCTGGCGCAGGAACGCGCGCCGGATGTGCGCTTCTCGGCCACCCGCGAACATGCGCATGTCAAGGCGGTCG
ATTGA

Upstream 100 bases:

>100_bases
CACGGCGCCCGCGCCTTCCACAAAAGCGTGCCGCAGCGAAGCCGCTATAATCGTCGGCTTCCTCAGTTTCCGCAGGCGTC
CGACCGGGCGCGCCCCGCCC

Downstream 100 bases:

>100_bases
CCTGCGCGTGACCTGCAGCAAGCGTGCGGGACCGCTGGCGATGCGAATCGCTCGCAATTGCGGTACCTTTTTGGCATGAA
CGCAGACGCCTCCCCCTCGA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSEVSNEAARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKGRKAARHATSDWMALEKERGISVTSSVMQFPYEGK
IVNLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEERTIKLMEVCRLRDTPIMTFINKLDREGKNPIDLLDEVETVL
GIQCAPVTWPIGMGQRLKGVVHLISGEVHLYEQGRNFTRQDSTIFPSLDAPGLVEKIGEQMLAELREELELVQGASNPFD
LDAYRAGQQTPVFFGSGVNNFGVQPLLDFFVEHAPPPQTRETTGRRVEPTEAKLSGFVFKIQANMDPQHRDRVAFMRVCS
GKFTAGMKTLHVRSGKDVKLANALTFMASDREIAAEAWPGDVIGIHNHGTISIGDTFTEGESLSFTGIPNFAPELFRRAR
LRDPLKLKQLQKGLAQLSEEGATQFFRPLMSNDLILGAVGVLQFDVVAYRLKDEYGVDAIFEPVSVTTARWVHCDNPKKL
EEFREKNAGNLGIDAAGELVYLAPTRVNLQLAQERAPDVRFSATREHAHVKAVD

Sequences:

>Translated_534_residues
MSEVSNEAARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKGRKAARHATSDWMALEKERGISVTSSVMQFPYEGK
IVNLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEERTIKLMEVCRLRDTPIMTFINKLDREGKNPIDLLDEVETVL
GIQCAPVTWPIGMGQRLKGVVHLISGEVHLYEQGRNFTRQDSTIFPSLDAPGLVEKIGEQMLAELREELELVQGASNPFD
LDAYRAGQQTPVFFGSGVNNFGVQPLLDFFVEHAPPPQTRETTGRRVEPTEAKLSGFVFKIQANMDPQHRDRVAFMRVCS
GKFTAGMKTLHVRSGKDVKLANALTFMASDREIAAEAWPGDVIGIHNHGTISIGDTFTEGESLSFTGIPNFAPELFRRAR
LRDPLKLKQLQKGLAQLSEEGATQFFRPLMSNDLILGAVGVLQFDVVAYRLKDEYGVDAIFEPVSVTTARWVHCDNPKKL
EEFREKNAGNLGIDAAGELVYLAPTRVNLQLAQERAPDVRFSATREHAHVKAVD
>Mature_533_residues
SEVSNEAARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKGRKAARHATSDWMALEKERGISVTSSVMQFPYEGKI
VNLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEERTIKLMEVCRLRDTPIMTFINKLDREGKNPIDLLDEVETVLG
IQCAPVTWPIGMGQRLKGVVHLISGEVHLYEQGRNFTRQDSTIFPSLDAPGLVEKIGEQMLAELREELELVQGASNPFDL
DAYRAGQQTPVFFGSGVNNFGVQPLLDFFVEHAPPPQTRETTGRRVEPTEAKLSGFVFKIQANMDPQHRDRVAFMRVCSG
KFTAGMKTLHVRSGKDVKLANALTFMASDREIAAEAWPGDVIGIHNHGTISIGDTFTEGESLSFTGIPNFAPELFRRARL
RDPLKLKQLQKGLAQLSEEGATQFFRPLMSNDLILGAVGVLQFDVVAYRLKDEYGVDAIFEPVSVTTARWVHCDNPKKLE
EFREKNAGNLGIDAAGELVYLAPTRVNLQLAQERAPDVRFSATREHAHVKAVD

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=481, Percent_Identity=28.0665280665281, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI19923640, Length=514, Percent_Identity=26.2645914396887, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI25306283, Length=336, Percent_Identity=29.4642857142857, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI25306287, Length=487, Percent_Identity=26.694045174538, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI157426893, Length=168, Percent_Identity=30.3571428571429, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI94966754, Length=148, Percent_Identity=29.7297297297297, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI4503483, Length=158, Percent_Identity=30.379746835443, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=64.1366223908918, Blast_Score=708, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=495, Percent_Identity=28.0808080808081, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI48994988, Length=175, Percent_Identity=34.2857142857143, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=521, Percent_Identity=27.6391554702495, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=164, Percent_Identity=36.5853658536585, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=149, Percent_Identity=32.2147651006711, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.0751879699248, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.0751879699248, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=31.1064718162839, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6322359, Length=426, Percent_Identity=27.4647887323944, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6324707, Length=161, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=161, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=27.536231884058, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582462, Length=490, Percent_Identity=28.1632653061224, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI221458488, Length=513, Percent_Identity=26.3157894736842, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI78706572, Length=157, Percent_Identity=32.484076433121, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=31.8181818181818, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=31.8181818181818, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=31.8181818181818, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59030; Mature: 58899

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVSNEAARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKGRKAARHATSDWMAL
CCCCHHHHHHCEEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCHHHHHH
EKERGISVTSSVMQFPYEGKIVNLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEER
HHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
TIKLMEVCRLRDTPIMTFINKLDREGKNPIDLLDEVETVLGIQCAPVTWPIGMGQRLKGV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
VHLISGEVHLYEQGRNFTRQDSTIFPSLDAPGLVEKIGEQMLAELREELELVQGASNPFD
HHHHCCCEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LDAYRAGQQTPVFFGSGVNNFGVQPLLDFFVEHAPPPQTRETTGRRVEPTEAKLSGFVFK
CHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCEEEEE
IQANMDPQHRDRVAFMRVCSGKFTAGMKTLHVRSGKDVKLANALTFMASDREIAAEAWPG
EECCCCHHHHHHHHHHHHHCCCHHCCEEEEEECCCCCEEHHHHEEEEECCCHHHHHCCCC
DVIGIHNHGTISIGDTFTEGESLSFTGIPNFAPELFRRARLRDPLKLKQLQKGLAQLSEE
CEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GATQFFRPLMSNDLILGAVGVLQFDVVAYRLKDEYGVDAIFEPVSVTTARWVHCDNPKKL
HHHHHHHHHHCCCEEEEHHHHHHHHHHHEECCCCCCCCHHCCCCEECEEEEEECCCHHHH
EEFREKNAGNLGIDAAGELVYLAPTRVNLQLAQERAPDVRFSATREHAHVKAVD
HHHHHCCCCCCCCCCCCCEEEEECCEEEEEEHHHCCCCCEEECCCCCCEEEECC
>Mature Secondary Structure 
SEVSNEAARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKGRKAARHATSDWMAL
CCCHHHHHHCEEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCHHHHHH
EKERGISVTSSVMQFPYEGKIVNLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEER
HHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
TIKLMEVCRLRDTPIMTFINKLDREGKNPIDLLDEVETVLGIQCAPVTWPIGMGQRLKGV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
VHLISGEVHLYEQGRNFTRQDSTIFPSLDAPGLVEKIGEQMLAELREELELVQGASNPFD
HHHHCCCEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LDAYRAGQQTPVFFGSGVNNFGVQPLLDFFVEHAPPPQTRETTGRRVEPTEAKLSGFVFK
CHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCEEEEE
IQANMDPQHRDRVAFMRVCSGKFTAGMKTLHVRSGKDVKLANALTFMASDREIAAEAWPG
EECCCCHHHHHHHHHHHHHCCCHHCCEEEEEECCCCCEEHHHHEEEEECCCHHHHHCCCC
DVIGIHNHGTISIGDTFTEGESLSFTGIPNFAPELFRRARLRDPLKLKQLQKGLAQLSEE
CEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GATQFFRPLMSNDLILGAVGVLQFDVVAYRLKDEYGVDAIFEPVSVTTARWVHCDNPKKL
HHHHHHHHHHCCCEEEEHHHHHHHHHHHEECCCCCCCCHHCCCCEECEEEEEECCCHHHH
EEFREKNAGNLGIDAAGELVYLAPTRVNLQLAQERAPDVRFSATREHAHVKAVD
HHHHHCCCCCCCCCCCCCEEEEECCEEEEEEHHHCCCCCEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA