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The map label for this gene is ispB [H]

Identifier: 21243633

GI number: 21243633

Start: 3412248

End: 3413246

Strand: Direct

Name: ispB [H]

Synonym: XAC2906

Alternate gene names: 21243633

Gene position: 3412248-3413246 (Clockwise)

Preceding gene: 77748680

Following gene: 21243639

Centisome position: 65.93

GC content: 67.67

Gene sequence:

>999_bases
ATGACCATCGCCGAAGACCTCCCCACCGTGCTGGGCCTGCCCCAGATCCAGTCCCTCGCCGCGCCCGACATGGCCGCGGT
CGACGCACTGATCCGCCGCCGTCTGGCCTCGGACGTCGTGCTGATCAACCAGATCGCCGATCACATCATCTCCGCCGGCG
GCAAACGCCTGCGCCCGATGCTGGTGATGCTGGCCGGCCATGCCGCTGGCGGCTCCGGCCCGGAGCACCACCAACTGGCG
GCAATCATCGAATTCATCCATACCTCCACGCTGCTGCACGACGACGTGGTGGACGAATCGGACCTGCGCCGCGGGCGCAG
CACCGCCAACGCGCTATGGGGCAATGCGCCCAGCGTATTGGTGGGCGACTTCCTGTACTCGCGTAGCTTCCAGTTGATGG
TCGAACTGGATCGCATGGAAGTCATGCAGATCCTGGCCGACACCACCAACCGCATCGCCGAAGGCGAAGTGCTTCAGCTG
TTGCATGTGCATAACCCCGACACCGACGAAGCGGCGTACCTGCGCGTGATCGAGCGCAAGACCGCAGTGCTGTTCGCCGC
CGGCACGCGTCTGGGTGCGCTTGCCTCCGGCGCAAGCGCGCAGGTGCAGCAGCAGCTGTACGACTACGGCATGCAGCTGG
GCTTCGCCTTCCAGATCGCCGACGACGTACTCGACTACGCCGCCGAGGCCGCGGACCTGGGCAAGAACCTGGGCGACGAT
CTTGCCGAGGGCAAGGCCACCCTGCCGCTGATCCACGCGATGGCGCACTCGGATGCGACCACCCGTGAGCGCCTGCGACA
GATCGTCGAGCAAGGCGATGCCGCGGCGATGCCGGAAGTGCTGGCCGCCATCCGTGCCACCGGCGGGTTGGACTACAGCC
GTCAACGCGCGGCCGAATACGCCGCCGCCGCCGAACAGGCTCTGGAAACCCTGCCGCACAGCGCTGCCGTGGCGGCGCTG
CGCGGCCTGGCGCGCTACGCGGTCGAGCGTACCCACTGA

Upstream 100 bases:

>100_bases
GCGACCACGTGAAGCCGCAAATGTGAAGACGCCGGTTGTGCCCGGCGGGACGGATCCGGCCGGGGCACCTATAATCGGCC
CACATCACGAACGCCTGCGC

Downstream 100 bases:

>100_bases
TCCGGAGCGCCCTGCGCGCCGGATGGGATTGAGCATTTGAGATTGGGGATTCGCAAGGGCGGCTAGTTCGCGCGTTGACG
AATCCCGCATCCGTTAGCCG

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTIAEDLPTVLGLPQIQSLAAPDMAAVDALIRRRLASDVVLINQIADHIISAGGKRLRPMLVMLAGHAAGGSGPEHHQLA
AIIEFIHTSTLLHDDVVDESDLRRGRSTANALWGNAPSVLVGDFLYSRSFQLMVELDRMEVMQILADTTNRIAEGEVLQL
LHVHNPDTDEAAYLRVIERKTAVLFAAGTRLGALASGASAQVQQQLYDYGMQLGFAFQIADDVLDYAAEAADLGKNLGDD
LAEGKATLPLIHAMAHSDATTRERLRQIVEQGDAAAMPEVLAAIRATGGLDYSRQRAAEYAAAAEQALETLPHSAAVAAL
RGLARYAVERTH

Sequences:

>Translated_332_residues
MTIAEDLPTVLGLPQIQSLAAPDMAAVDALIRRRLASDVVLINQIADHIISAGGKRLRPMLVMLAGHAAGGSGPEHHQLA
AIIEFIHTSTLLHDDVVDESDLRRGRSTANALWGNAPSVLVGDFLYSRSFQLMVELDRMEVMQILADTTNRIAEGEVLQL
LHVHNPDTDEAAYLRVIERKTAVLFAAGTRLGALASGASAQVQQQLYDYGMQLGFAFQIADDVLDYAAEAADLGKNLGDD
LAEGKATLPLIHAMAHSDATTRERLRQIVEQGDAAAMPEVLAAIRATGGLDYSRQRAAEYAAAAEQALETLPHSAAVAAL
RGLARYAVERTH
>Mature_331_residues
TIAEDLPTVLGLPQIQSLAAPDMAAVDALIRRRLASDVVLINQIADHIISAGGKRLRPMLVMLAGHAAGGSGPEHHQLAA
IIEFIHTSTLLHDDVVDESDLRRGRSTANALWGNAPSVLVGDFLYSRSFQLMVELDRMEVMQILADTTNRIAEGEVLQLL
HVHNPDTDEAAYLRVIERKTAVLFAAGTRLGALASGASAQVQQQLYDYGMQLGFAFQIADDVLDYAAEAADLGKNLGDDL
AEGKATLPLIHAMAHSDATTRERLRQIVEQGDAAAMPEVLAAIRATGGLDYSRQRAAEYAAAAEQALETLPHSAAVAALR
GLARYAVERTH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=306, Percent_Identity=30.3921568627451, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4758430, Length=266, Percent_Identity=25.187969924812, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI83700220, Length=266, Percent_Identity=25.187969924812, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=51.7133956386293, Blast_Score=324, Evalue=5e-90,
Organism=Escherichia coli, GI1786623, Length=241, Percent_Identity=33.6099585062241, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17505681, Length=291, Percent_Identity=31.9587628865979, Blast_Score=160, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6319475, Length=302, Percent_Identity=32.7814569536424, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325188, Length=241, Percent_Identity=25.7261410788382, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24651612, Length=297, Percent_Identity=29.96632996633, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281365769, Length=257, Percent_Identity=26.4591439688716, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24660002, Length=257, Percent_Identity=26.4591439688716, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35622; Mature: 35491

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAEDLPTVLGLPQIQSLAAPDMAAVDALIRRRLASDVVLINQIADHIISAGGKRLRPM
CCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVMLAGHAAGGSGPEHHQLAAIIEFIHTSTLLHDDVVDESDLRRGRSTANALWGNAPSVL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHCCCCCHHH
VGDFLYSRSFQLMVELDRMEVMQILADTTNRIAEGEVLQLLHVHNPDTDEAAYLRVIERK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHH
TAVLFAAGTRLGALASGASAQVQQQLYDYGMQLGFAFQIADDVLDYAAEAADLGKNLGDD
HHHEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LAEGKATLPLIHAMAHSDATTRERLRQIVEQGDAAAMPEVLAAIRATGGLDYSRQRAAEY
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
AAAAEQALETLPHSAAVAALRGLARYAVERTH
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIAEDLPTVLGLPQIQSLAAPDMAAVDALIRRRLASDVVLINQIADHIISAGGKRLRPM
CCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVMLAGHAAGGSGPEHHQLAAIIEFIHTSTLLHDDVVDESDLRRGRSTANALWGNAPSVL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHCCCCCHHH
VGDFLYSRSFQLMVELDRMEVMQILADTTNRIAEGEVLQLLHVHNPDTDEAAYLRVIERK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHH
TAVLFAAGTRLGALASGASAQVQQQLYDYGMQLGFAFQIADDVLDYAAEAADLGKNLGDD
HHHEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LAEGKATLPLIHAMAHSDATTRERLRQIVEQGDAAAMPEVLAAIRATGGLDYSRQRAAEY
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
AAAAEQALETLPHSAAVAALRGLARYAVERTH
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]