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The map label for this gene is gltX

Identifier: 21243574

GI number: 21243574

Start: 3338819

End: 3340222

Strand: Reverse

Name: gltX

Synonym: XAC2847

Alternate gene names: 21243574

Gene position: 3340222-3338819 (Counterclockwise)

Preceding gene: 21243575

Following gene: 21243573

Centisome position: 64.54

GC content: 64.46

Gene sequence:

>1404_bases
ATGACCTGCCGCACCCGTTTCGCTCCCAGCCCCACCGGTTACCTGCATATCGGCGGCGCCCGCACCGCCTTGTATTGCTG
GCTGGAGGCACGCCACCGCGGCGGCCAGTTCGTGCTGCGCATCGAGGACACCGACCGCGAGCGCAGCACGCAGGAAGCGA
TCGACGCCATCCTGGAAGCGATGGACTGGCTGGGGCTGGGTTACGACGAAGGCCCGATCTACCAGACCCAGCGCGTTGCC
CGCTATCAAGAAGTCGCCGAGCAATTGCTCGCGCAGGGCAAGGCGTATTACGCCTACGAAACCCGCGAGGAACTCGACGC
CATGCGCGAGGCCGCCATGGCCAAGCAGGAAAAGCCGCGTTACAACGGCGCTGCCCGCGAGCAGAACCTGCCGTATCGCG
ACGACCCCAATCGCGTGATCCGCTTCAAGAATCCCGTCGGCGGTACGGTGGTGTTCGACGACCTGATCAAGGGACGCATC
GAGATCGCCAACAGCGAGCTCGACGACATGGTGATCTTCCGCCCGGACGGGTTTCCCACCTACAACTTTGCGGTGGTGGT
GGACGACTGGGACATGGGCATCACCGAGGTCATCCGCGGCGACGACCACATCAACAACACCCCGCGCCAGATCAATATCT
ATGAAGCGCTGGATGCACCGGTGCCCAAGTTCGCGCACATGCCGATGATCCTCGACGAGCAGGGCGCCAAGCTGTCCAAG
CGCACCGGCGCGGCCGACGTCATGCAGTACAAGGATGCCGGCTATCTGCCGCATGCGCTGATCAATTATCTGGCCCGCCT
GGGCTGGTCGCATGGCGACCAGGAGCTGTTCACCCAGCAGGAGTTGCTGGACCTGTTCGACGTCAAGGACGTCAATTCCA
AGGCCGCGCGCCTGGACATGGCCAAGCTCGGCTGGGTCAATCAGCATTATCTGAAGACCGACGACCCGGCCAGCATCGCC
CCGCAGTTGGAATACCAGCTTGCCAAGCTCGGTGTGGACATCGCCGCCGGCCCGGCCGCCGCCGATGTGGTGGTGGCGTT
GCGCGAGCGCGTGCAGACCTTGAAGGAAATGGCCGAAAAAGCCGTGGTCTGGTACCAGCCGCTGGAAACCTATGACGAAG
CGGCGGTGATGAAACACCTCAAGCTGGGCGCCGAGGTGCCGCTGGGCAAGGCGCGCGAACTGCTGGCTGCCGTGGATGCG
TGGAGCGTGGAGAGCGTCTCGGCTGCGCTGCACGACGCCGCTGCCGCGCTGGAACTGGGCATGGGCAAGGTCGCCCAGCC
GCTGCGCGTGGCCATCACCGGCACCCAGGTCAGCCCGGACATTTCGCAAACGGTGTACCTGGCCGGACGCGAGGGTGCCT
TGAAACGCATCGATGCGGCACTCATCAAGATTGGAGCGGCCTGA

Upstream 100 bases:

>100_bases
TGGTGTACGGCGACCTGCTGCGCAACCGCTGAGCCACGGGCCGGGCAGGGCCACGCTGCGCAAGGTAAAATCGGGCGACT
CCCGTTTTGCCGAGCGCTCC

Downstream 100 bases:

>100_bases
CGCCGGAGCCTGTATGACCAAGCACACCCACGCACCAGACCTCGCCTGCACTGCACCGCATCACCACGTCGACGACGCCA
ACGGCTTCGTGCGCGCGGTG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTCRTRFAPSPTGYLHIGGARTALYCWLEARHRGGQFVLRIEDTDRERSTQEAIDAILEAMDWLGLGYDEGPIYQTQRVA
RYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPRYNGAAREQNLPYRDDPNRVIRFKNPVGGTVVFDDLIKGRI
EIANSELDDMVIFRPDGFPTYNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYEALDAPVPKFAHMPMILDEQGAKLSK
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTQQELLDLFDVKDVNSKAARLDMAKLGWVNQHYLKTDDPASIA
PQLEYQLAKLGVDIAAGPAAADVVVALRERVQTLKEMAEKAVVWYQPLETYDEAAVMKHLKLGAEVPLGKARELLAAVDA
WSVESVSAALHDAAAALELGMGKVAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALIKIGAA

Sequences:

>Translated_467_residues
MTCRTRFAPSPTGYLHIGGARTALYCWLEARHRGGQFVLRIEDTDRERSTQEAIDAILEAMDWLGLGYDEGPIYQTQRVA
RYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPRYNGAAREQNLPYRDDPNRVIRFKNPVGGTVVFDDLIKGRI
EIANSELDDMVIFRPDGFPTYNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYEALDAPVPKFAHMPMILDEQGAKLSK
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTQQELLDLFDVKDVNSKAARLDMAKLGWVNQHYLKTDDPASIA
PQLEYQLAKLGVDIAAGPAAADVVVALRERVQTLKEMAEKAVVWYQPLETYDEAAVMKHLKLGAEVPLGKARELLAAVDA
WSVESVSAALHDAAAALELGMGKVAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALIKIGAA
>Mature_466_residues
TCRTRFAPSPTGYLHIGGARTALYCWLEARHRGGQFVLRIEDTDRERSTQEAIDAILEAMDWLGLGYDEGPIYQTQRVAR
YQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPRYNGAAREQNLPYRDDPNRVIRFKNPVGGTVVFDDLIKGRIE
IANSELDDMVIFRPDGFPTYNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYEALDAPVPKFAHMPMILDEQGAKLSKR
TGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTQQELLDLFDVKDVNSKAARLDMAKLGWVNQHYLKTDDPASIAP
QLEYQLAKLGVDIAAGPAAADVVVALRERVQTLKEMAEKAVVWYQPLETYDEAAVMKHLKLGAEVPLGKARELLAAVDAW
SVESVSAALHDAAAALELGMGKVAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALIKIGAA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=326, Percent_Identity=36.8098159509202, Blast_Score=211, Evalue=2e-54,
Organism=Escherichia coli, GI1788743, Length=461, Percent_Identity=54.4468546637744, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=38.8429752066116, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1786895, Length=216, Percent_Identity=26.8518518518519, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=475, Percent_Identity=29.4736842105263, Blast_Score=189, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6324540, Length=500, Percent_Identity=31.2, Blast_Score=236, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=209, Percent_Identity=28.2296650717703, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=109, Percent_Identity=33.9449541284404, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24665321, Length=499, Percent_Identity=31.8637274549098, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_XANAC (Q8PIP8)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643156.1
- ProteinModelPortal:   Q8PIP8
- SMR:   Q8PIP8
- GeneID:   1156918
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2847
- NMPDR:   fig|190486.1.peg.2800
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   XAXO190486:XAC2847-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51861; Mature: 51729

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCRTRFAPSPTGYLHIGGARTALYCWLEARHRGGQFVLRIEDTDRERSTQEAIDAILEA
CCCCCCCCCCCCCEEEECCCCEEHEEEHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHH
MDWLGLGYDEGPIYQTQRVARYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCC
YNGAAREQNLPYRDDPNRVIRFKNPVGGTVVFDDLIKGRIEIANSELDDMVIFRPDGFPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEHHHHHCHHEECCCCCCCEEEECCCCCCC
YNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYEALDAPVPKFAHMPMILDEQGAKLSK
EEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEHHHHCCCCCCHHCCCEEECCCCCCHHH
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTQQELLDLFDVKDVNSKAARLDM
HCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
AKLGWVNQHYLKTDDPASIAPQLEYQLAKLGVDIAAGPAAADVVVALRERVQTLKEMAEK
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHH
AVVWYQPLETYDEAAVMKHLKLGAEVPLGKARELLAAVDAWSVESVSAALHDAAAALELG
HEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MGKVAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALIKIGAA
CCHHCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TCRTRFAPSPTGYLHIGGARTALYCWLEARHRGGQFVLRIEDTDRERSTQEAIDAILEA
CCCCCCCCCCCCEEEECCCCEEHEEEHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHH
MDWLGLGYDEGPIYQTQRVARYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCC
YNGAAREQNLPYRDDPNRVIRFKNPVGGTVVFDDLIKGRIEIANSELDDMVIFRPDGFPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEHHHHHCHHEECCCCCCCEEEECCCCCCC
YNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYEALDAPVPKFAHMPMILDEQGAKLSK
EEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEHHHHCCCCCCHHCCCEEECCCCCCHHH
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTQQELLDLFDVKDVNSKAARLDM
HCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
AKLGWVNQHYLKTDDPASIAPQLEYQLAKLGVDIAAGPAAADVVVALRERVQTLKEMAEK
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHH
AVVWYQPLETYDEAAVMKHLKLGAEVPLGKARELLAAVDAWSVESVSAALHDAAAALELG
HEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MGKVAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALIKIGAA
CCHHCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217