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The map label for this gene is phoA [H]

Identifier: 21243486

GI number: 21243486

Start: 3230960

End: 3232666

Strand: Direct

Name: phoA [H]

Synonym: XAC2759

Alternate gene names: 21243486

Gene position: 3230960-3232666 (Clockwise)

Preceding gene: 21243482

Following gene: 21243487

Centisome position: 62.43

GC content: 68.83

Gene sequence:

>1707_bases
ATGCCGATGCGTTACCGCCTGCCTACCCTCGCCGCCCTGACCACGCTGTGCGTCGCTGCCTGCGCTTCCACCACCGGGAG
CGGCACCTCCGCACCCGCGTCGGTCCGCGTGGAGGTGCCGCCGGTCACGCACCCGGCCGGCGAAACCCCGCAATGGTGGT
ACCGCTCGGGCGCCGCCCGCGCGGCCGGCAACGGCGCCATGGCCGGCAAGGCGCGCAACGTGATCCTGTTCCTGGGCGAT
GGCATGAGCCTGACCACGGTGGCCGCCGCGCGCATCCTGGACGGCCAGCGCAAGGGCGGCCCAGGCGAAGAGAACCTGCT
GTCGTGGGAGCAGTTTCCCGCCACCGCCTTCAGCAAGACCTACAACACCGACTCGCAGACGCCGGATTCTGCCGGCACCA
TGACCTCCATCACCACCGGCGTGAAGTCGCACATGGGCGCGATCGGCGTCAGCAGCGGCAACCGCAGCGACTGCGCCGAC
AGCTTGAACAAGGGCCTGCTGAGCTGGCTGCAGCTGGCCGACAGCGCCGGCATGGCCACCGGCATCGTCACCACCACGCG
CCTGACCCATGCCACGCCGGCGGCAACCTATGCGCATTCGCCGGACCGCAACTGGGAAAACGACACCGACCTTCCCGAGT
CGGCGCGTGCTGCCGGTTGCCAGGACATTGCCCAGCAATTGCTCTCCACCGCCCGCTACGGCCGAGGCCCGCAGGTGGTG
CTGGGCGGAGGACGCAGCCAGTTCCAGAGCGTGCAGGAGCGCGACCCGGAATACGACGACAAGGTGGGCCTGCGCCTGGA
CGGTCGCGATCTGGTCGCCGAGTGGAAACAAGCGCACCCGCAGGGCGCCTATGTCTGGAACGAGCAGCAATTGCAGGCAG
CGTCCGGTGCACCTGCGCTGCTGGGGCTGTTCGAACCGGACCACATGCAGTTCGATCACGACCGCAACCGCACCCCGCAG
GGCGAGCCCAGCCTGACCGAGATGACCCGCACCGCGATCCAGTCGCTGTCGCGCGAGCCGAATGGGTTCGTACTGATGGT
CGAAGGCGGCCGCATCGATCACGCCAACCACGCCGGCAACGCCTACCGCGCGCTCGATGAAACCGTGTCGCTGTCCGATG
CCGTGCGCGTTGCTGTGCAGACTGCGCCACCGGACACGCTGATCATCGTCACCGCCGACCATTCGCACACGCTCAACTTC
GTCGGCTACCCGGTGCGCGGTAACCCGATCCTGGGCAAGGTGCGCGGCACCGGCGGCGAAGAAGGCGACCGCAGCCAGCT
GGCCACCGACCTGGCCGGCCAGCCGTACACCACGCTCAGCTACGCCAACGGCCCCGGCTATACAGGTGCCAGCAACGCGC
AGCCGGCCGGTCCGAAGAAGTATCCGCACGAACCCAGCAGCAGCGAGCCGGCCACTGGCCGCCCGGACCTGAGCCATGTC
GACACCGAAGCCCCCAGCTACATGCAGGAAGCGCTGGTGCCGACCAAATCCGAAAGCCACGGCGGCGAAGATGTCGGCAT
CTGGGCCACCGGCCCCGGCAGCGCGGCATTCCGCGGCACGCTGGAAGAGAACGTGATCTACCACGTCATCGTCCAGGCCA
CTCCGCGCCTGCGCGAGCGCCTGTGCAAGGCCGGCACCTGCAATGCCGCTGGTGTGCCGGTGGAATTGCCCAAGCCGGCC
ACGTTCGAGGCTGCGGTCAAGCGCTGA

Upstream 100 bases:

>100_bases
GGGCTGCCCTGCTCGCGAGACCACAGCTGGAACAGCGCTGTGTCATCGGTGTGACACCACCCAGCGCGGCATAATGGGGA
TTGATTCAGTCACGAGTTCG

Downstream 100 bases:

>100_bases
TGCGCAGGCGGCGCTGCAGCGATGGCGGGCGAGCCGGCGCATGATGCGGTCGACGCATCGCCGCTCGACCTGCCTGCCTG
GCGTCGCCAATGCCGTGTGC

Product: alkaline phosphatase

Products: NA

Alternate protein names: High molecular weight phosphatase; H-AP [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MPMRYRLPTLAALTTLCVAACASTTGSGTSAPASVRVEVPPVTHPAGETPQWWYRSGAARAAGNGAMAGKARNVILFLGD
GMSLTTVAAARILDGQRKGGPGEENLLSWEQFPATAFSKTYNTDSQTPDSAGTMTSITTGVKSHMGAIGVSSGNRSDCAD
SLNKGLLSWLQLADSAGMATGIVTTTRLTHATPAATYAHSPDRNWENDTDLPESARAAGCQDIAQQLLSTARYGRGPQVV
LGGGRSQFQSVQERDPEYDDKVGLRLDGRDLVAEWKQAHPQGAYVWNEQQLQAASGAPALLGLFEPDHMQFDHDRNRTPQ
GEPSLTEMTRTAIQSLSREPNGFVLMVEGGRIDHANHAGNAYRALDETVSLSDAVRVAVQTAPPDTLIIVTADHSHTLNF
VGYPVRGNPILGKVRGTGGEEGDRSQLATDLAGQPYTTLSYANGPGYTGASNAQPAGPKKYPHEPSSSEPATGRPDLSHV
DTEAPSYMQEALVPTKSESHGGEDVGIWATGPGSAAFRGTLEENVIYHVIVQATPRLRERLCKAGTCNAAGVPVELPKPA
TFEAAVKR

Sequences:

>Translated_568_residues
MPMRYRLPTLAALTTLCVAACASTTGSGTSAPASVRVEVPPVTHPAGETPQWWYRSGAARAAGNGAMAGKARNVILFLGD
GMSLTTVAAARILDGQRKGGPGEENLLSWEQFPATAFSKTYNTDSQTPDSAGTMTSITTGVKSHMGAIGVSSGNRSDCAD
SLNKGLLSWLQLADSAGMATGIVTTTRLTHATPAATYAHSPDRNWENDTDLPESARAAGCQDIAQQLLSTARYGRGPQVV
LGGGRSQFQSVQERDPEYDDKVGLRLDGRDLVAEWKQAHPQGAYVWNEQQLQAASGAPALLGLFEPDHMQFDHDRNRTPQ
GEPSLTEMTRTAIQSLSREPNGFVLMVEGGRIDHANHAGNAYRALDETVSLSDAVRVAVQTAPPDTLIIVTADHSHTLNF
VGYPVRGNPILGKVRGTGGEEGDRSQLATDLAGQPYTTLSYANGPGYTGASNAQPAGPKKYPHEPSSSEPATGRPDLSHV
DTEAPSYMQEALVPTKSESHGGEDVGIWATGPGSAAFRGTLEENVIYHVIVQATPRLRERLCKAGTCNAAGVPVELPKPA
TFEAAVKR
>Mature_567_residues
PMRYRLPTLAALTTLCVAACASTTGSGTSAPASVRVEVPPVTHPAGETPQWWYRSGAARAAGNGAMAGKARNVILFLGDG
MSLTTVAAARILDGQRKGGPGEENLLSWEQFPATAFSKTYNTDSQTPDSAGTMTSITTGVKSHMGAIGVSSGNRSDCADS
LNKGLLSWLQLADSAGMATGIVTTTRLTHATPAATYAHSPDRNWENDTDLPESARAAGCQDIAQQLLSTARYGRGPQVVL
GGGRSQFQSVQERDPEYDDKVGLRLDGRDLVAEWKQAHPQGAYVWNEQQLQAASGAPALLGLFEPDHMQFDHDRNRTPQG
EPSLTEMTRTAIQSLSREPNGFVLMVEGGRIDHANHAGNAYRALDETVSLSDAVRVAVQTAPPDTLIIVTADHSHTLNFV
GYPVRGNPILGKVRGTGGEEGDRSQLATDLAGQPYTTLSYANGPGYTGASNAQPAGPKKYPHEPSSSEPATGRPDLSHVD
TEAPSYMQEALVPTKSESHGGEDVGIWATGPGSAAFRGTLEENVIYHVIVQATPRLRERLCKAGTCNAAGVPVELPKPAT
FEAAVKR

Specific function: Has only a phosphomonoesterase activity [H]

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Secreted. Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=520, Percent_Identity=41.7307692307692, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI94721246, Length=520, Percent_Identity=41.9230769230769, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI157266292, Length=497, Percent_Identity=41.851106639839, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI116734717, Length=473, Percent_Identity=42.2832980972516, Blast_Score=352, Evalue=5e-97,
Organism=Homo sapiens, GI294660770, Length=468, Percent_Identity=41.8803418803419, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI294660772, Length=436, Percent_Identity=39.4495412844037, Blast_Score=297, Evalue=2e-80,
Organism=Escherichia coli, GI48994877, Length=362, Percent_Identity=32.0441988950276, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320689, Length=355, Percent_Identity=31.830985915493, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21355149, Length=486, Percent_Identity=40.5349794238683, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI21355151, Length=473, Percent_Identity=42.4947145877378, Blast_Score=312, Evalue=5e-85,
Organism=Drosophila melanogaster, GI24651554, Length=519, Percent_Identity=39.8843930635838, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI21358067, Length=469, Percent_Identity=41.5778251599147, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI85725200, Length=487, Percent_Identity=40.041067761807, Blast_Score=302, Evalue=5e-82,
Organism=Drosophila melanogaster, GI85815833, Length=487, Percent_Identity=40.041067761807, Blast_Score=302, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24657835, Length=514, Percent_Identity=38.3268482490272, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI19921912, Length=539, Percent_Identity=38.0333951762523, Blast_Score=297, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24585213, Length=544, Percent_Identity=37.5, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI21355981, Length=472, Percent_Identity=39.1949152542373, Blast_Score=288, Evalue=7e-78,
Organism=Drosophila melanogaster, GI24657842, Length=513, Percent_Identity=38.401559454191, Blast_Score=285, Evalue=7e-77,
Organism=Drosophila melanogaster, GI24651556, Length=476, Percent_Identity=40.7563025210084, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24657827, Length=502, Percent_Identity=40.2390438247012, Blast_Score=282, Evalue=5e-76,
Organism=Drosophila melanogaster, GI18859923, Length=487, Percent_Identity=37.9876796714579, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24645593, Length=298, Percent_Identity=27.8523489932886, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 60088; Mature: 59957

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMRYRLPTLAALTTLCVAACASTTGSGTSAPASVRVEVPPVTHPAGETPQWWYRSGAAR
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCC
AAGNGAMAGKARNVILFLGDGMSLTTVAAARILDGQRKGGPGEENLLSWEQFPATAFSKT
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHCCCHHHCCCHHHHHC
YNTDSQTPDSAGTMTSITTGVKSHMGAIGVSSGNRSDCADSLNKGLLSWLQLADSAGMAT
CCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
GIVTTTRLTHATPAATYAHSPDRNWENDTDLPESARAAGCQDIAQQLLSTARYGRGPQVV
EEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCEEE
LGGGRSQFQSVQERDPEYDDKVGLRLDGRDLVAEWKQAHPQGAYVWNEQQLQAASGAPAL
ECCCHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHCCCCCCEEECHHHHHHCCCCCCE
LGLFEPDHMQFDHDRNRTPQGEPSLTEMTRTAIQSLSREPNGFVLMVEGGRIDHANHAGN
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCH
AYRALDETVSLSDAVRVAVQTAPPDTLIIVTADHSHTLNFVGYPVRGNPILGKVRGTGGE
HHHHHHHHHHHCHHEEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCC
EGDRSQLATDLAGQPYTTLSYANGPGYTGASNAQPAGPKKYPHEPSSSEPATGRPDLSHV
CCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DTEAPSYMQEALVPTKSESHGGEDVGIWATGPGSAAFRGTLEENVIYHVIVQATPRLRER
CCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHCCEEEEEEECCCHHHHHH
LCKAGTCNAAGVPVELPKPATFEAAVKR
HHCCCCCCCCCCCEECCCCCCHHHHHCC
>Mature Secondary Structure 
PMRYRLPTLAALTTLCVAACASTTGSGTSAPASVRVEVPPVTHPAGETPQWWYRSGAAR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCC
AAGNGAMAGKARNVILFLGDGMSLTTVAAARILDGQRKGGPGEENLLSWEQFPATAFSKT
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHCCCHHHCCCHHHHHC
YNTDSQTPDSAGTMTSITTGVKSHMGAIGVSSGNRSDCADSLNKGLLSWLQLADSAGMAT
CCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
GIVTTTRLTHATPAATYAHSPDRNWENDTDLPESARAAGCQDIAQQLLSTARYGRGPQVV
EEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCEEE
LGGGRSQFQSVQERDPEYDDKVGLRLDGRDLVAEWKQAHPQGAYVWNEQQLQAASGAPAL
ECCCHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHCCCCCCEEECHHHHHHCCCCCCE
LGLFEPDHMQFDHDRNRTPQGEPSLTEMTRTAIQSLSREPNGFVLMVEGGRIDHANHAGN
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCH
AYRALDETVSLSDAVRVAVQTAPPDTLIIVTADHSHTLNFVGYPVRGNPILGKVRGTGGE
HHHHHHHHHHHCHHEEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCC
EGDRSQLATDLAGQPYTTLSYANGPGYTGASNAQPAGPKKYPHEPSSSEPATGRPDLSHV
CCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DTEAPSYMQEALVPTKSESHGGEDVGIWATGPGSAAFRGTLEENVIYHVIVQATPRLRER
CCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHCCEEEEEEECCCHHHHHH
LCKAGTCNAAGVPVELPKPATFEAAVKR
HHCCCCCCCCCCCEECCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 8454193 [H]