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The map label for this gene is mesJ

Identifier: 21243487

GI number: 21243487

Start: 3233015

End: 3234337

Strand: Direct

Name: mesJ

Synonym: XAC2760

Alternate gene names: 21243487

Gene position: 3233015-3234337 (Clockwise)

Preceding gene: 21243486

Following gene: 21243488

Centisome position: 62.47

GC content: 69.69

Gene sequence:

>1323_bases
ATGCGCTGCGAGGTCGCGCCTGTGCTGACCGGCACCCCGCTGCTGCCAGCCACCCCGCCTGGCCCGGTGCTGCTGGCCTA
CAGCGGCGGGATGGATTCCAGCGTACTGCTACATCTGCTGGCGGCGAGGCCGCGCTATCGACACGCAGGATTGCGCGCGC
TGCATGTGCATCATGGGTTGCACGCCGATGCCGACGCCTGGGCGGCGCATTGCCAACGCACGTGCGACGCGCTGCAGGTG
CCGTTGCAGATCGTGCGGGTGCAGGTGGCACGCGACAGCGGGCTTGGGCTGGAAGCGGCAGCACGCAATGCGCGCCACGC
GGCTTTTGCGCAGGCCCTCATTGCTGGCGAATGGCTGGCGCTGGCGCATCATCGCGACGACCAGGCGGAAACGTTCCTGC
TGCGTGCGCTGCGCGCGTCCGGCCCCGAAGGCCTGGCGGCGATGCGCCCGCAGCGCCCGTTCGCCAGCGGCACGTTGTGG
CGCCCGATGCTGGCGCATGCGCGCGCCGATCTGCTTGCCTACGCGCATGCGCACCGATTGCGCTGGATCGATGACCCCAG
CAACACCGACCCACGCCACGACCGCAATTTCCTGCGCTCCCAGGTGTTGCCGTTGCTGCAGCAACGCTGGCCGCAGGCGA
CCGACGCGCTGGCGCGCAGTGCGCAACTGAGCGCCGATGCCAGCGCGTTGCTGCTGCAGCAAGACATGGCGTTGCTGCCA
GGCGTGCTTACCGCCAGCGGCGCGCTGGATCTGCAAGCATTGCGCGCGCAATCTGTGGAACGAAGGGCAAGGCTGTTGCG
CGCCTGGGTGAGTGCGGCGCACGCGCCGCCGCTGCCTGCGCACGGCGTGGCCGCACTGGAGCAGGAAATCGACAATCACG
CTGCAGATCGGCACGCCTGCTTTGCGTGGCAGCAGGTAGAAGTACGCCGCTGGCGCCAGTGCCTGTTTCTGCATCGCCCC
GCTGCGGCCTGGCCGTCGGACTGGCACGCGCAGTGGGACGGCGCAGCGCCCCTGCAACTCCCCGACGGTGCGCAGTTGCG
GTTGCTCGGCGCACCGGGCCTGCGCTTTGCGCAACCGCTGCTGGTTCGGGCCCGCCAGGGCGGCGAGCGCATCGTGTTGC
CGCAGCGCGGGCATTCGCACCAACTCAAACATCTGCTGCAGGCGCTGGATCTACCGCCGTGGGAACGCGAGCGCCTGCCG
GTCCTGTGGGAAGGCGCGCAGGTGCTCGCCGCCGGCGACTGCATCATTTCCGGCACCTTGGACGCATGGCTGCAGGCCAA
TGCCGCAGCGCTGCAGTGGCGCACGGCTGCCGATGCGAATTGA

Upstream 100 bases:

>100_bases
CACTGGAATCGTCGCTGTATCTTCAGCAGGCAACACCGCAGGCAGGCGACCGACCGGCCATCACGCAGCCGGGACGGCAT
CTGCTCACCAGGCGCGCAGG

Downstream 100 bases:

>100_bases
CCCGCCTGCGCGCGCGCCGCACACTTTAGCGATGGCCAAGAAGTCCTTGAACGAAACGTCCCCGGTTGCCCGCTTCGAAC
AGTCGCTGGAAGAACTCGAG

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MRCEVAPVLTGTPLLPATPPGPVLLAYSGGMDSSVLLHLLAARPRYRHAGLRALHVHHGLHADADAWAAHCQRTCDALQV
PLQIVRVQVARDSGLGLEAAARNARHAAFAQALIAGEWLALAHHRDDQAETFLLRALRASGPEGLAAMRPQRPFASGTLW
RPMLAHARADLLAYAHAHRLRWIDDPSNTDPRHDRNFLRSQVLPLLQQRWPQATDALARSAQLSADASALLLQQDMALLP
GVLTASGALDLQALRAQSVERRARLLRAWVSAAHAPPLPAHGVAALEQEIDNHAADRHACFAWQQVEVRRWRQCLFLHRP
AAAWPSDWHAQWDGAAPLQLPDGAQLRLLGAPGLRFAQPLLVRARQGGERIVLPQRGHSHQLKHLLQALDLPPWERERLP
VLWEGAQVLAAGDCIISGTLDAWLQANAAALQWRTAADAN

Sequences:

>Translated_440_residues
MRCEVAPVLTGTPLLPATPPGPVLLAYSGGMDSSVLLHLLAARPRYRHAGLRALHVHHGLHADADAWAAHCQRTCDALQV
PLQIVRVQVARDSGLGLEAAARNARHAAFAQALIAGEWLALAHHRDDQAETFLLRALRASGPEGLAAMRPQRPFASGTLW
RPMLAHARADLLAYAHAHRLRWIDDPSNTDPRHDRNFLRSQVLPLLQQRWPQATDALARSAQLSADASALLLQQDMALLP
GVLTASGALDLQALRAQSVERRARLLRAWVSAAHAPPLPAHGVAALEQEIDNHAADRHACFAWQQVEVRRWRQCLFLHRP
AAAWPSDWHAQWDGAAPLQLPDGAQLRLLGAPGLRFAQPLLVRARQGGERIVLPQRGHSHQLKHLLQALDLPPWERERLP
VLWEGAQVLAAGDCIISGTLDAWLQANAAALQWRTAADAN
>Mature_440_residues
MRCEVAPVLTGTPLLPATPPGPVLLAYSGGMDSSVLLHLLAARPRYRHAGLRALHVHHGLHADADAWAAHCQRTCDALQV
PLQIVRVQVARDSGLGLEAAARNARHAAFAQALIAGEWLALAHHRDDQAETFLLRALRASGPEGLAAMRPQRPFASGTLW
RPMLAHARADLLAYAHAHRLRWIDDPSNTDPRHDRNFLRSQVLPLLQQRWPQATDALARSAQLSADASALLLQQDMALLP
GVLTASGALDLQALRAQSVERRARLLRAWVSAAHAPPLPAHGVAALEQEIDNHAADRHACFAWQQVEVRRWRQCLFLHRP
AAAWPSDWHAQWDGAAPLQLPDGAQLRLLGAPGLRFAQPLLVRARQGGERIVLPQRGHSHQLKHLLQALDLPPWERERLP
VLWEGAQVLAAGDCIISGTLDAWLQANAAALQWRTAADAN

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=410, Percent_Identity=39.5121951219512, Blast_Score=253, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_XANAC (Q8PIY5)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643069.1
- ProteinModelPortal:   Q8PIY5
- SMR:   Q8PIY5
- GeneID:   1156831
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2760
- NMPDR:   fig|190486.1.peg.2713
- HOGENOM:   HBG668702
- OMA:   WWIKSVA
- ProtClustDB:   CLSK446060
- BioCyc:   XAXO190486:XAC2760-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 48232; Mature: 48232

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCEVAPVLTGTPLLPATPPGPVLLAYSGGMDSSVLLHLLAARPRYRHAGLRALHVHHGL
CCCEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHCCCEEEEECCCC
HADADAWAAHCQRTCDALQVPLQIVRVQVARDSGLGLEAAARNARHAAFAQALIAGEWLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAHHRDDQAETFLLRALRASGPEGLAAMRPQRPFASGTLWRPMLAHARADLLAYAHAHRL
HHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RWIDDPSNTDPRHDRNFLRSQVLPLLQQRWPQATDALARSAQLSADASALLLQQDMALLP
EECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GVLTASGALDLQALRAQSVERRARLLRAWVSAAHAPPLPAHGVAALEQEIDNHAADRHAC
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHH
FAWQQVEVRRWRQCLFLHRPAAAWPSDWHAQWDGAAPLQLPDGAQLRLLGAPGLRFAQPL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHH
LVRARQGGERIVLPQRGHSHQLKHLLQALDLPPWERERLPVLWEGAQVLAAGDCIISGTL
HHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEECCEEEECCH
DAWLQANAAALQWRTAADAN
HHHHHCCHHHEEEECCCCCC
>Mature Secondary Structure
MRCEVAPVLTGTPLLPATPPGPVLLAYSGGMDSSVLLHLLAARPRYRHAGLRALHVHHGL
CCCEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHCCCEEEEECCCC
HADADAWAAHCQRTCDALQVPLQIVRVQVARDSGLGLEAAARNARHAAFAQALIAGEWLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAHHRDDQAETFLLRALRASGPEGLAAMRPQRPFASGTLWRPMLAHARADLLAYAHAHRL
HHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RWIDDPSNTDPRHDRNFLRSQVLPLLQQRWPQATDALARSAQLSADASALLLQQDMALLP
EECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GVLTASGALDLQALRAQSVERRARLLRAWVSAAHAPPLPAHGVAALEQEIDNHAADRHAC
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHH
FAWQQVEVRRWRQCLFLHRPAAAWPSDWHAQWDGAAPLQLPDGAQLRLLGAPGLRFAQPL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHH
LVRARQGGERIVLPQRGHSHQLKHLLQALDLPPWERERLPVLWEGAQVLAAGDCIISGTL
HHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEECCEEEECCH
DAWLQANAAALQWRTAADAN
HHHHHCCHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217