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The map label for this gene is queA

Identifier: 21243247

GI number: 21243247

Start: 2943476

End: 2944546

Strand: Reverse

Name: queA

Synonym: XAC2514

Alternate gene names: 21243247

Gene position: 2944546-2943476 (Counterclockwise)

Preceding gene: 21243248

Following gene: 21243246

Centisome position: 56.89

GC content: 68.25

Gene sequence:

>1071_bases
TTGAAGAAGTCCGATTTCCATTACGACCTGCCCGACGAACTGATCGCCCAGGCGCCGCTGGCCGAGCGCGCGGCGAGCCG
GTTGCTGGTGGTGCCGCCATCGCCGCAGGCGCTGGCCGACCGCCAGGTGCGCGACCTGCCCGAGCTGCTGCAGCCCGGCG
ATCTGCTGATCTTCAACGACACCCGGGTCATTCCGGCGCGCTTGTTCGGGCAGAAGGCCAGCGGCGGGCGGGTGGAAATC
CTGATCGAGCGCCTGCTTGGCGAGCGTCAGGCGCGGGTACAGATCGGCGCCAGCAAGTCGCCCAAGGCCGGCAGCCTGAT
CGCGCTCGATGCCGGTGGCCAGGCCGAGGTGCTGGGCCGCGATGGCGAGTTCTATCTGCTGCGTTTCGAGATCCCGACCC
CGCTGGAGCACTGGCTGCTGGAGGCTGGCCGCCTGCCGCTGCCGCCCTACATCCGCCGCGAGCCGGGCGTGGAAGACCGC
GAACGCTATCAGACCGTGTTCGCGCGCGAAGTCGGTGCGGTGGCCGCGCCCACCGCCGGGCTGCATTTCGACGAGCCGCT
GCTGGCACGCTTGCGCGAGCGCGGGGTGGAGTTCGGCCACGTCACCTTGCATGTGGGTGCCGGCACCTTTCAGCCGGTGC
GGGTGGACAAGCTCGACCAGCATGTGATGCACAAGGAGTGGCTCAACGTCGGGGCGGCGCTGGTGGAACAGGTGCGCCGC
ACGCGTGCCCGCGGCGGCCGCGTGATCGCGGTGGGCACCACGGTGGTGCGCTCGCTGGAAAGCGCCTGGCGCAAGACCGA
GGCCGCGCCCGAAGGCGAATTGCAGCCATTCGCCGGCGAAACCCAGATCTTCATCCTGCCCGGCTACCGCATCCGCAGCG
TCGATGCGATGGTCACCAACTTCCATCTGCCCGAAAGCACCTTGATGATGATGGTCTCTGCCTTCGCCGGCCGCGAGCGC
ATCTTCGCCGCCTACCACCACGCCATCGCGCAGCGCTACCGCTTCTTTTCGTACGGCGATGCGATGTTGTTGTGGGGCCG
GGAATCGGGACTCGGGAATGGGGAATCGTAA

Upstream 100 bases:

>100_bases
GGAATCGGAAGCGCTGCCGCCCGTCCGATTCCCCAGTCCCAATTCTCGATTCCCGGCCGCAGTTACACTATGCGGCTACC
CAGACAACGATCCCGCGGCC

Downstream 100 bases:

>100_bases
GGCGGGACCCGCCGAACGATCAGCCACCTGCCAACCGCTCTTCCCATTCCCGATTCCCCACTCCCCACTCCCCATTCCCG
GCCCCTCAATGTCCCGACTC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKKSDFHYDLPDELIAQAPLAERAASRLLVVPPSPQALADRQVRDLPELLQPGDLLIFNDTRVIPARLFGQKASGGRVEI
LIERLLGERQARVQIGASKSPKAGSLIALDAGGQAEVLGRDGEFYLLRFEIPTPLEHWLLEAGRLPLPPYIRREPGVEDR
ERYQTVFAREVGAVAAPTAGLHFDEPLLARLRERGVEFGHVTLHVGAGTFQPVRVDKLDQHVMHKEWLNVGAALVEQVRR
TRARGGRVIAVGTTVVRSLESAWRKTEAAPEGELQPFAGETQIFILPGYRIRSVDAMVTNFHLPESTLMMMVSAFAGRER
IFAAYHHAIAQRYRFFSYGDAMLLWGRESGLGNGES

Sequences:

>Translated_356_residues
MKKSDFHYDLPDELIAQAPLAERAASRLLVVPPSPQALADRQVRDLPELLQPGDLLIFNDTRVIPARLFGQKASGGRVEI
LIERLLGERQARVQIGASKSPKAGSLIALDAGGQAEVLGRDGEFYLLRFEIPTPLEHWLLEAGRLPLPPYIRREPGVEDR
ERYQTVFAREVGAVAAPTAGLHFDEPLLARLRERGVEFGHVTLHVGAGTFQPVRVDKLDQHVMHKEWLNVGAALVEQVRR
TRARGGRVIAVGTTVVRSLESAWRKTEAAPEGELQPFAGETQIFILPGYRIRSVDAMVTNFHLPESTLMMMVSAFAGRER
IFAAYHHAIAQRYRFFSYGDAMLLWGRESGLGNGES
>Mature_356_residues
MKKSDFHYDLPDELIAQAPLAERAASRLLVVPPSPQALADRQVRDLPELLQPGDLLIFNDTRVIPARLFGQKASGGRVEI
LIERLLGERQARVQIGASKSPKAGSLIALDAGGQAEVLGRDGEFYLLRFEIPTPLEHWLLEAGRLPLPPYIRREPGVEDR
ERYQTVFAREVGAVAAPTAGLHFDEPLLARLRERGVEFGHVTLHVGAGTFQPVRVDKLDQHVMHKEWLNVGAALVEQVRR
TRARGGRVIAVGTTVVRSLESAWRKTEAAPEGELQPFAGETQIFILPGYRIRSVDAMVTNFHLPESTLMMMVSAFAGRER
IFAAYHHAIAQRYRFFSYGDAMLLWGRESGLGNGES

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=52.8901734104046, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_XANAC (Q8PJL6)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642829.1
- ProteinModelPortal:   Q8PJL6
- SMR:   Q8PJL6
- GeneID:   1156585
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2514
- NMPDR:   fig|190486.1.peg.2473
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   XAXO190486:XAC2514-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39579; Mature: 39579

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSDFHYDLPDELIAQAPLAERAASRLLVVPPSPQALADRQVRDLPELLQPGDLLIFND
CCCCCCCCCCCHHHHHCCCHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECC
TRVIPARLFGQKASGGRVEILIERLLGERQARVQIGASKSPKAGSLIALDAGGQAEVLGR
CCEEHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCEEEEEC
DGEFYLLRFEIPTPLEHWLLEAGRLPLPPYIRREPGVEDRERYQTVFAREVGAVAAPTAG
CCCEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
LHFDEPLLARLRERGVEFGHVTLHVGAGTFQPVRVDKLDQHVMHKEWLNVGAALVEQVRR
CCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TRARGGRVIAVGTTVVRSLESAWRKTEAAPEGELQPFAGETQIFILPGYRIRSVDAMVTN
HHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHC
FHLPESTLMMMVSAFAGRERIFAAYHHAIAQRYRFFSYGDAMLLWGRESGLGNGES
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKKSDFHYDLPDELIAQAPLAERAASRLLVVPPSPQALADRQVRDLPELLQPGDLLIFND
CCCCCCCCCCCHHHHHCCCHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECC
TRVIPARLFGQKASGGRVEILIERLLGERQARVQIGASKSPKAGSLIALDAGGQAEVLGR
CCEEHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCEEEEEC
DGEFYLLRFEIPTPLEHWLLEAGRLPLPPYIRREPGVEDRERYQTVFAREVGAVAAPTAG
CCCEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
LHFDEPLLARLRERGVEFGHVTLHVGAGTFQPVRVDKLDQHVMHKEWLNVGAALVEQVRR
CCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TRARGGRVIAVGTTVVRSLESAWRKTEAAPEGELQPFAGETQIFILPGYRIRSVDAMVTN
HHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHC
FHLPESTLMMMVSAFAGRERIFAAYHHAIAQRYRFFSYGDAMLLWGRESGLGNGES
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217