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The map label for this gene is tgt

Identifier: 21243246

GI number: 21243246

Start: 2942242

End: 2943387

Strand: Reverse

Name: tgt

Synonym: XAC2513

Alternate gene names: 21243246

Gene position: 2943387-2942242 (Counterclockwise)

Preceding gene: 21243247

Following gene: 21243245

Centisome position: 56.87

GC content: 66.4

Gene sequence:

>1146_bases
ATGTCCCGACTCCAGTTCCAACTCCAAGCCACCGACGGGCATGCCCGGCGTGGGCGCCTGACCTTTCCGCGCGGCACGGT
CGAAACGCCTGCGTTCATGCCGGTCGGGACCTATGGCTCGGTCAAGGGCATCCTGCCCGAGCAGATCCGCGCGCTGGGTG
CGGAGATCATCCTGGGCAACACCTTCCATCTGTATCTGCGCCCGGGTCTGGAGGTGATCGGCGATCACGGCGGGCTGCAC
GGGTTTGCGCGCTGGGATGGGCCCATCCTCACCGATTCCGGCGGCTTCCAGGTGTTTTCGCTGGCGCATCGCCGCAAGAT
CACCGAGCAGGGCGTGACCTTTTCCTCGCCGACCGATGGCGCGCGGGTGTTCCTGGGCCCCGAAGAGAGCATGAAAATTC
AAAAGGTGCTCGATTCGGACATCGTGATGATCTTCGACGAGTGCACGCCGTACCCGGCCACCGAGGACGTCGCGCGCCGC
TCGATGGAGCTGAGCCTGCGCTGGGCGCAGCGCTCGCGCGATGCGCACGACGGGCTGGGCAACGATGCGGCGCTGTTCGG
CATCGTGCAGGGTGGGGTGCATCCGGATCTGCGCAGCCGCTCGCTGGACGGCCTGCAAGCCATTGGTTTCGATGGGTATG
CGATCGGTGGGCTGGCAGTGGGCGAGCCCGAGCACGAACGCAATGCCATGCTCGAACACCTGCATCCGCGCCTGCCGGCC
GAGCGCCCGCGCTACCTGATGGGCGTGGGCCGCCCGGAAGACCTGGTCGAAGGCGTGGCCCGCGGGGTGGACATGTTCGA
CTGCGTCATGCCCACCCGCAACGCGCGCAACGGCCACTATTTCACCTCGTTCGGCACCGTGCGCATCCGCAATGCCAAGT
ACGAGCGCGACCTGGACACCATCGAGCCGGGCTGCGGTTGCCATGCCTGCAGCAGCGGCTATACGCGCGCCTACCTGCGC
CATCTGGACCGCTGCAACGAAATGCTGGCGCCGATGCTGGGCACCCTGCACAACCTCTGGTACTACGAAAAACTGATGGC
CGACATGCGCGCAGCGATCGCTTCGGGAACCTTTGTGGAGTTCCGGCGGTCCTTCTATGCGGCACGCGGCGCCACCACAC
CGCCGTTGCCGGGGGAAACCAGCTGA

Upstream 100 bases:

>100_bases
GGGGAATCGTAAGGCGGGACCCGCCGAACGATCAGCCACCTGCCAACCGCTCTTCCCATTCCCGATTCCCCACTCCCCAC
TCCCCATTCCCGGCCCCTCA

Downstream 100 bases:

>100_bases
GACCGGCCGCCCGCGCCCCGGCGCGGACGGCGTGGAACCTGCGTGGCATAATTCCCGGCTGTTCCGCCCCGGCGGTACCT
GAATAGCGAGCGGGCCCATG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSRLQFQLQATDGHARRGRLTFPRGTVETPAFMPVGTYGSVKGILPEQIRALGAEIILGNTFHLYLRPGLEVIGDHGGLH
GFARWDGPILTDSGGFQVFSLAHRRKITEQGVTFSSPTDGARVFLGPEESMKIQKVLDSDIVMIFDECTPYPATEDVARR
SMELSLRWAQRSRDAHDGLGNDAALFGIVQGGVHPDLRSRSLDGLQAIGFDGYAIGGLAVGEPEHERNAMLEHLHPRLPA
ERPRYLMGVGRPEDLVEGVARGVDMFDCVMPTRNARNGHYFTSFGTVRIRNAKYERDLDTIEPGCGCHACSSGYTRAYLR
HLDRCNEMLAPMLGTLHNLWYYEKLMADMRAAIASGTFVEFRRSFYAARGATTPPLPGETS

Sequences:

>Translated_381_residues
MSRLQFQLQATDGHARRGRLTFPRGTVETPAFMPVGTYGSVKGILPEQIRALGAEIILGNTFHLYLRPGLEVIGDHGGLH
GFARWDGPILTDSGGFQVFSLAHRRKITEQGVTFSSPTDGARVFLGPEESMKIQKVLDSDIVMIFDECTPYPATEDVARR
SMELSLRWAQRSRDAHDGLGNDAALFGIVQGGVHPDLRSRSLDGLQAIGFDGYAIGGLAVGEPEHERNAMLEHLHPRLPA
ERPRYLMGVGRPEDLVEGVARGVDMFDCVMPTRNARNGHYFTSFGTVRIRNAKYERDLDTIEPGCGCHACSSGYTRAYLR
HLDRCNEMLAPMLGTLHNLWYYEKLMADMRAAIASGTFVEFRRSFYAARGATTPPLPGETS
>Mature_380_residues
SRLQFQLQATDGHARRGRLTFPRGTVETPAFMPVGTYGSVKGILPEQIRALGAEIILGNTFHLYLRPGLEVIGDHGGLHG
FARWDGPILTDSGGFQVFSLAHRRKITEQGVTFSSPTDGARVFLGPEESMKIQKVLDSDIVMIFDECTPYPATEDVARRS
MELSLRWAQRSRDAHDGLGNDAALFGIVQGGVHPDLRSRSLDGLQAIGFDGYAIGGLAVGEPEHERNAMLEHLHPRLPAE
RPRYLMGVGRPEDLVEGVARGVDMFDCVMPTRNARNGHYFTSFGTVRIRNAKYERDLDTIEPGCGCHACSSGYTRAYLRH
LDRCNEMLAPMLGTLHNLWYYEKLMADMRAAIASGTFVEFRRSFYAARGATTPPLPGETS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=360, Percent_Identity=43.6111111111111, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1786607, Length=373, Percent_Identity=64.0750670241287, Blast_Score=503, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17544680, Length=363, Percent_Identity=40.495867768595, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI25146502, Length=142, Percent_Identity=28.8732394366197, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19920510, Length=370, Percent_Identity=42.4324324324324, Blast_Score=291, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_XANAC (Q8PJL7)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642828.1
- ProteinModelPortal:   Q8PJL7
- SMR:   Q8PJL7
- GeneID:   1156584
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2513
- NMPDR:   fig|190486.1.peg.2472
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   XAXO190486:XAC2513-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42126; Mature: 41995

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: ACT_SITE 92-92 BINDING 93-93

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLQFQLQATDGHARRGRLTFPRGTVETPAFMPVGTYGSVKGILPEQIRALGAEIILGN
CCCEEEEEEECCCCCCCCEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECC
TFHLYLRPGLEVIGDHGGLHGFARWDGPILTDSGGFQVFSLAHRRKITEQGVTFSSPTDG
EEEEEEECCCCEECCCCCCCEEEECCCCEEECCCCCEEHHHHHHHHHHHCCCEECCCCCC
ARVFLGPEESMKIQKVLDSDIVMIFDECTPYPATEDVARRSMELSLRWAQRSRDAHDGLG
CEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCC
NDAALFGIVQGGVHPDLRSRSLDGLQAIGFDGYAIGGLAVGEPEHERNAMLEHLHPRLPA
CCCEEEEEECCCCCCCHHHCCCCCHHEECCCCEEECCEECCCCCHHHHHHHHHHCCCCCC
ERPRYLMGVGRPEDLVEGVARGVDMFDCVMPTRNARNGHYFTSFGTVRIRNAKYERDLDT
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECEEEEECCCCCCCCCC
IEPGCGCHACSSGYTRAYLRHLDRCNEMLAPMLGTLHNLWYYEKLMADMRAAIASGTFVE
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FRRSFYAARGATTPPLPGETS
HHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SRLQFQLQATDGHARRGRLTFPRGTVETPAFMPVGTYGSVKGILPEQIRALGAEIILGN
CCEEEEEEECCCCCCCCEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECC
TFHLYLRPGLEVIGDHGGLHGFARWDGPILTDSGGFQVFSLAHRRKITEQGVTFSSPTDG
EEEEEEECCCCEECCCCCCCEEEECCCCEEECCCCCEEHHHHHHHHHHHCCCEECCCCCC
ARVFLGPEESMKIQKVLDSDIVMIFDECTPYPATEDVARRSMELSLRWAQRSRDAHDGLG
CEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCC
NDAALFGIVQGGVHPDLRSRSLDGLQAIGFDGYAIGGLAVGEPEHERNAMLEHLHPRLPA
CCCEEEEEECCCCCCCHHHCCCCCHHEECCCCEEECCEECCCCCHHHHHHHHHHCCCCCC
ERPRYLMGVGRPEDLVEGVARGVDMFDCVMPTRNARNGHYFTSFGTVRIRNAKYERDLDT
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECEEEEECCCCCCCCCC
IEPGCGCHACSSGYTRAYLRHLDRCNEMLAPMLGTLHNLWYYEKLMADMRAAIASGTFVE
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FRRSFYAARGATTPPLPGETS
HHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217