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The map label for this gene is hisF

Identifier: 21242578

GI number: 21242578

Start: 2129301

End: 2130077

Strand: Direct

Name: hisF

Synonym: XAC1834

Alternate gene names: 21242578

Gene position: 2129301-2130077 (Clockwise)

Preceding gene: 21242577

Following gene: 21242579

Centisome position: 41.14

GC content: 64.09

Gene sequence:

>777_bases
ATGCTGAGCCGTCGCATCATTCCGTGCCTGGACGTGCGCGATGGGCGCGTGGTCAAGGGCGTCAAGTTCCGCGATCACAT
CGACATGGGCGACATCGTCGAACTGGCATTGCGCTACCGCGCGCAGGGCGCCGACGAGCTGGTGTTCTACGACATCGGTG
CCAGCCCGGAAGGACGCTCGGTCGACTACACCTGGGTCGAGCGGGTGGCGCGGCTGATCGATATTCCGTTCTGCGTGGCC
GGCGGTATTGGCGATGTGGAAACAGCGCGCGCGGTGCTGCACGCCGGTGCCGACAAGATATCGATCAACTCGCCCGCGCT
TGGCCGCCCGCAATTGATTTCCGAGCTGGCCGATGCCTTTGGCGTGCAATGTGTCGTGGTCGGCATCGACTCGATTCGTG
AGGAAGATGGTCAGTGGCGCGTGCGCCGCTACACCGGCGATCCGAGCAAGACCCAGGCGTTGCCGATGCGCACGCTGGAT
TGGGTGGCCGAAGCGCAGCGGCTGGGCGCAGGGGAAATGGTGCTCAATTGCATGGACAACGACGGCGTGCGTCGCGGCTA
CGACATCGCCCAGTTGCGGCAGGTGCGCGCGTTATGCCGCGTGCCGCTGATCGCCTCCGGCGGTGCAGGCGACATGCAGC
ACTTCGCCGATGTGTTCGACCAGGCCGATGTGGATGGCGCACTGGCGGCCAGCGTATTTCACAGTGGTGCGATTCCGATT
CCGGAACTCAAGCAATTCTTGCGCGCGCAACAGATCGAGGTACGCGATGGGCAGTAA

Upstream 100 bases:

>100_bases
ATCTGGCCGATGTCGCAGCTGCCAAGGCCGCGGGCTGCGCCGGCATCGTGCTGGGCAAGGCCTTGCTCGAAGGGCATCTG
AATCTCGACGAGGCACTGGC

Downstream 100 bases:

>100_bases
CGAAGTGGCGACGGGCGACCCGCTCGCAACGCTGGATTGGAACAAGGGCGAGGGCCTGTTGCCGGTGATCGTGCAGGACG
CCGACAACCTGCGCGTGCTG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLSRRIIPCLDVRDGRVVKGVKFRDHIDMGDIVELALRYRAQGADELVFYDIGASPEGRSVDYTWVERVARLIDIPFCVA
GGIGDVETARAVLHAGADKISINSPALGRPQLISELADAFGVQCVVVGIDSIREEDGQWRVRRYTGDPSKTQALPMRTLD
WVAEAQRLGAGEMVLNCMDNDGVRRGYDIAQLRQVRALCRVPLIASGGAGDMQHFADVFDQADVDGALAASVFHSGAIPI
PELKQFLRAQQIEVRDGQ

Sequences:

>Translated_258_residues
MLSRRIIPCLDVRDGRVVKGVKFRDHIDMGDIVELALRYRAQGADELVFYDIGASPEGRSVDYTWVERVARLIDIPFCVA
GGIGDVETARAVLHAGADKISINSPALGRPQLISELADAFGVQCVVVGIDSIREEDGQWRVRRYTGDPSKTQALPMRTLD
WVAEAQRLGAGEMVLNCMDNDGVRRGYDIAQLRQVRALCRVPLIASGGAGDMQHFADVFDQADVDGALAASVFHSGAIPI
PELKQFLRAQQIEVRDGQ
>Mature_258_residues
MLSRRIIPCLDVRDGRVVKGVKFRDHIDMGDIVELALRYRAQGADELVFYDIGASPEGRSVDYTWVERVARLIDIPFCVA
GGIGDVETARAVLHAGADKISINSPALGRPQLISELADAFGVQCVVVGIDSIREEDGQWRVRRYTGDPSKTQALPMRTLD
WVAEAQRLGAGEMVLNCMDNDGVRRGYDIAQLRQVRALCRVPLIASGGAGDMQHFADVFDQADVDGALAASVFHSGAIPI
PELKQFLRAQQIEVRDGQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=64.0625, Blast_Score=329, Evalue=9e-92,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=30.6709265175719, Blast_Score=127, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_XANAC (Q8PLG6)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642160.1
- ProteinModelPortal:   Q8PLG6
- SMR:   Q8PLG6
- GeneID:   1155905
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1834
- NMPDR:   fig|190486.1.peg.1804
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- ProtClustDB:   PRK02083
- BioCyc:   XAXO190486:XAC1834-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28259; Mature: 28259

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRRIIPCLDVRDGRVVKGVKFRDHIDMGDIVELALRYRAQGADELVFYDIGASPEGRS
CCCCCCCCCEECCCCCEEECCCHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
VDYTWVERVARLIDIPFCVAGGIGDVETARAVLHAGADKISINSPALGRPQLISELADAF
CCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCVVVGIDSIREEDGQWRVRRYTGDPSKTQALPMRTLDWVAEAQRLGAGEMVLNCMDN
CCEEEEEECHHHHCCCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHCCCCHHEEEECCC
DGVRRGYDIAQLRQVRALCRVPLIASGGAGDMQHFADVFDQADVDGALAASVFHSGAIPI
CCCCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCH
PELKQFLRAQQIEVRDGQ
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLSRRIIPCLDVRDGRVVKGVKFRDHIDMGDIVELALRYRAQGADELVFYDIGASPEGRS
CCCCCCCCCEECCCCCEEECCCHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
VDYTWVERVARLIDIPFCVAGGIGDVETARAVLHAGADKISINSPALGRPQLISELADAF
CCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCVVVGIDSIREEDGQWRVRRYTGDPSKTQALPMRTLDWVAEAQRLGAGEMVLNCMDN
CCEEEEEECHHHHCCCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHCCCCHHEEEECCC
DGVRRGYDIAQLRQVRALCRVPLIASGGAGDMQHFADVFDQADVDGALAASVFHSGAIPI
CCCCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCH
PELKQFLRAQQIEVRDGQ
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217