Click here to switch to the map view.

The map label for this gene is ppnK

Identifier: 21242345

GI number: 21242345

Start: 1841786

End: 1842562

Strand: Direct

Name: ppnK

Synonym: XAC1595

Alternate gene names: 21242345

Gene position: 1841786-1842562 (Clockwise)

Preceding gene: 21242344

Following gene: 21242346

Centisome position: 35.59

GC content: 65.89

Gene sequence:

>777_bases
ATGACCGCACCACCTCGCATCGCTTTTCTTGCCAGCCCCGCCGAGCCGGCGGTGGCCGCGCGTGCGCGCCTTACGCAGCG
CTATGGCGACCATGCGCTGGACAGCGCCGACATCGTCTGTGCGCTGGGCGGCGATGGGTTCATGCTGCAAACGCTGCACC
GGCATGGCGCCTCCGACAAGCCGGTGTTCGGCATGAAGCTGGGCTCGGTCGGCTTTTTGATGAACCAGTACCGCGACAAC
GAGGACGACCTGCTCGAGCGCCTGCAGCGCGCCGAGCCGGCGTATCTGCGGCCGCTGGAAATGCAGGTGCAGACCGAATC
CGGGGCCAGTGCTGGCTCGCTGGCCTACAACGAGGTCTCGCTGTTGCGGCAGACCCGGCAGGCCGCGCATCTCAGCATTG
ATCTCAACGGCCAGACCCGCATCGCCGAGCTGATCGGCGATGGCGTGATGGTGGCCACGCCGGCCGGCAGCACGGCGTAC
AACTATTCCGCGCACGGGCCGATCCTGCCGCTGGGCTCGCATACGTTGGCGCTGACGCCGATCGCACCGTATCGTCCGCG
ACGCTGGCGCGGCGCCATCCTCAAGGCAGATACCGAAGTGCGCTTTCGCGTGCTCGACCCGTACAAGCGCCCGGTCAGCG
TCACCGCCGACTCGCACGAAATTCGCGACGTGGTCGAAGTGACCATCCGCGAATCCGCGCAGCGCCAGGTCACGTTGTTG
TTCGATCCCGAACACAATCTCGAAGAGCGCATCTTCAGCGAGCAGTTTGCGGTATGA

Upstream 100 bases:

>100_bases
TGCCGTGCATGAGGGGGAGGGTGGGACATGAGCGCAACGGACCCACAACAAGCCCAGCACGGGGCTGCGGTATGCTGATT
CGCATGCTCTGGAGTCAACG

Downstream 100 bases:

>100_bases
AAGGCGGGGATCTGGGATTCGAGATTCGGGATTGGCAACGGCAGCAGCGTGCCACGTCGTCTTTAGAATCGCGCGCACGC
GCTACGATGCTGCGCGGATG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTAPPRIAFLASPAEPAVAARARLTQRYGDHALDSADIVCALGGDGFMLQTLHRHGASDKPVFGMKLGSVGFLMNQYRDN
EDDLLERLQRAEPAYLRPLEMQVQTESGASAGSLAYNEVSLLRQTRQAAHLSIDLNGQTRIAELIGDGVMVATPAGSTAY
NYSAHGPILPLGSHTLALTPIAPYRPRRWRGAILKADTEVRFRVLDPYKRPVSVTADSHEIRDVVEVTIRESAQRQVTLL
FDPEHNLEERIFSEQFAV

Sequences:

>Translated_258_residues
MTAPPRIAFLASPAEPAVAARARLTQRYGDHALDSADIVCALGGDGFMLQTLHRHGASDKPVFGMKLGSVGFLMNQYRDN
EDDLLERLQRAEPAYLRPLEMQVQTESGASAGSLAYNEVSLLRQTRQAAHLSIDLNGQTRIAELIGDGVMVATPAGSTAY
NYSAHGPILPLGSHTLALTPIAPYRPRRWRGAILKADTEVRFRVLDPYKRPVSVTADSHEIRDVVEVTIRESAQRQVTLL
FDPEHNLEERIFSEQFAV
>Mature_257_residues
TAPPRIAFLASPAEPAVAARARLTQRYGDHALDSADIVCALGGDGFMLQTLHRHGASDKPVFGMKLGSVGFLMNQYRDNE
DDLLERLQRAEPAYLRPLEMQVQTESGASAGSLAYNEVSLLRQTRQAAHLSIDLNGQTRIAELIGDGVMVATPAGSTAYN
YSAHGPILPLGSHTLALTPIAPYRPRRWRGAILKADTEVRFRVLDPYKRPVSVTADSHEIRDVVEVTIRESAQRQVTLLF
DPEHNLEERIFSEQFAV

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=192, Percent_Identity=30.7291666666667, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325068, Length=177, Percent_Identity=29.9435028248588, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320794, Length=188, Percent_Identity=29.7872340425532, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_XANAC (Q8PM39)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641927.1
- ProteinModelPortal:   Q8PM39
- GeneID:   1155666
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1595
- NMPDR:   fig|190486.1.peg.1571
- HOGENOM:   HBG294544
- OMA:   VIHPLLM
- ProtClustDB:   PRK04761
- BioCyc:   XAXO190486:XAC1595-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 28429; Mature: 28297

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPPRIAFLASPAEPAVAARARLTQRYGDHALDSADIVCALGGDGFMLQTLHRHGASDK
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCC
PVFGMKLGSVGFLMNQYRDNEDDLLERLQRAEPAYLRPLEMQVQTESGASAGSLAYNEVS
CEEEEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHH
LLRQTRQAAHLSIDLNGQTRIAELIGDGVMVATPAGSTAYNYSAHGPILPLGSHTLALTP
HHHHHHHHEEEEEEECCCHHHHHHHCCCEEEEECCCCCEECCCCCCCEEECCCCEEEEEC
IAPYRPRRWRGAILKADTEVRFRVLDPYKRPVSVTADSHEIRDVVEVTIRESAQRQVTLL
CCCCCCCCCCCEEEEECCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEE
FDPEHNLEERIFSEQFAV
ECCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TAPPRIAFLASPAEPAVAARARLTQRYGDHALDSADIVCALGGDGFMLQTLHRHGASDK
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCC
PVFGMKLGSVGFLMNQYRDNEDDLLERLQRAEPAYLRPLEMQVQTESGASAGSLAYNEVS
CEEEEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHH
LLRQTRQAAHLSIDLNGQTRIAELIGDGVMVATPAGSTAYNYSAHGPILPLGSHTLALTP
HHHHHHHHEEEEEEECCCHHHHHHHCCCEEEEECCCCCEECCCCCCCEEECCCCEEEEEC
IAPYRPRRWRGAILKADTEVRFRVLDPYKRPVSVTADSHEIRDVVEVTIRESAQRQVTLL
CCCCCCCCCCCEEEEECCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEE
FDPEHNLEERIFSEQFAV
ECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217