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The map label for this gene is rlmL

Identifier: 21242344

GI number: 21242344

Start: 1839434

End: 1841569

Strand: Direct

Name: rlmL

Synonym: XAC1594

Alternate gene names: 21242344

Gene position: 1839434-1841569 (Clockwise)

Preceding gene: 21242343

Following gene: 21242345

Centisome position: 35.54

GC content: 67.51

Gene sequence:

>2136_bases
GTGAAATTCTTCGCATCCTGCGCCAAGGGCCTGGAATACCTGCTTGCCGACGAGCTGCTTGCGCTCGGTGCCAGCAGGGC
CACTGCCACTATTTCCGGCGTCAACGTCGAAGGCGAACTGCGCGATGCGCAGCGCGCCGTGCTGTGGTCGCGCCTGGCCA
GCCGCGTGCTGTGGCCGCTGACCGAGTTCGATTGCCCGGACGAAGACGCGCTGTACGCCGGCGTGGCCGAACTGCCATGG
GACGCGCATCTGTCGGTGGGGCATACGCTGTCGGTGGACGCGCATGTGTCCGGGACCGCCATCACCCACGCCCGCTACGC
GGCGCAGCGCATCAAGGACGCGGTGGTCGACACCATGCGCCGGCAGGGGCTGGAGCGCCCGTCGGTGGACGTGGAAAACC
CGGACCTGCGCCTGAACCTGTCGCTGCGCAAGGGCCGCGCCACCATTTCGGTGGACCTGGGCGGCGGCCCGCTGCATCGT
CGCGGCTGGCGCATGGCGCAGAACGAGGCGCCGTTGAAGGAAAATCTGGCCGCAGCGGTACTGATGCGCGGCGGCTGGCC
GCGCGCCTATGCCGATGGCGGCGGTCTACTCGACCCGATGTGCGGCAGCGGCACGCTGCTGATCGAAGGCGCGCTGATGG
CCGCCGACGTCGCGCCCGGTCTGCAGCGCTACGGCAGCGATGTGCCCAGCCGCTGGCGCGGTTTCGACCGCGACGGCTGG
CAGCAGCTGGTCGGTGAGGCGCGCGAACGCGATAGGGTCGGCCGCGCTGCGCTCAAGCAGGTGATCCATGGCAGCGATAT
GGACCCGCACGCCATCCGCGCTGCGAAAGAGAATGCGCAAGTGGCCGGCGTGGCCGAGGCGATCTGGTTCGGCGTGCGCG
AGGTCGGTGAATTGCAGACCCCGCCGCAGGCCACCGGCGTGGTGGTGTGCAACCCGCCCTACGACGAGCGCCTGGCCGCC
GATGCGGCGCTGTATCGCCGCCTGGGCGACACCTTGCAGCGCGTCGCGCCGCAGTGGCGTGCCAGCCTGCTGTGCGGCAA
TGCCGAGCTGGCGTATGCCACCGGCCTGCGCGCGGGCAAGAAATACCAGCTGTTCAACGGTGCGATCGAATGCGCGTTGA
TCGTCTGCGACCCGATTGCAGTGCCGCGGCGTACCCCGCTGGCGGCACCAACCGCGCTCAGCGAGGGCGCGCAGATGGTG
GCCAACCGGCTGCGCAAGAATCTGCAGAAATTCAAGAAGTGGCGCGCGCGCGAAGGCATCGAATGTTTCCGCGCCTACGA
CGCCGACCTGCCGGAATATTCCGCCGCCATCGACGTGTACCAGCAGGCCGATGGCGACCGGCGCATCTTCCTGCATGTGC
AGGAATACGCAGCGCCGGCGACGATTCCCGAGGCCGATGTGCGTCGTCGCCTCAACGAATTGCTGGCCGCCGCACGCGAG
GTCTTCGAGGTACCGGCCGAGCGCGTGGCGCTGAAGTCGCGCGAACGCGGCAAGGGCGGCAGCAAGTACGGCCGCTTCGA
GCAACGCAACGAAATCGTCCACGTGCGCGAACACGGCGCGTTGCTGCGCGTGAACCTGTTCGATTATCTGGATACCGGGC
TGTTCCTGGACCATCGCCCGCTGCGCGGCACCATGGCGCAGCAGTGCAGGGGGCGCCGTTTCCTCAACCTGTTCTGCTAC
ACCGGTGTGGCCAGCGTGGAAGCGGCGGTGGCCGGTGCGGCCTCCACCACCAGCGTGGATCTGTCTGCAACGTACCTGCA
GTGGTGCGCCGATAATCTTGCGTTGAACGGGCTGGCCGGGAGCAAGCACAAACTGGTGCAGGCCGATGCATTGGCCTGGC
TGGAAGCCGAGCGTGCGCACTTCGATGTGATCTTCTGCGACCCACCGACCTTCTCCAATTCTGCGCGCGCGGAAGATTTC
GATATCCAGCGCGAGCACGTGCGTCTGTTGCGCGCGGCCGTGGCACGGTTGGCGCCGGGGGGGGTGTTGTATTTCTCCAA
TAACTTCCGTCGCTTCAAGCTGGACGAGGAAGGGGTGGCGGAGTTCGCGCAGTGCGAAGAAATCAGCCCCCGCACCATCG
ACCCGGACTTCGAGCGGCATGCGCGCATCCATCGCGCCTGGCGGTTGACGGCCTGA

Upstream 100 bases:

>100_bases
CCGTCCACGGCGGCCGCGGCATTGCCCACCAGGGAGCCGGCCCGCCGTCCTGCTTTCACGCTCGCCGCCGCGGCCAGCGT
CTTTATCGATACCTGCCCAC

Downstream 100 bases:

>100_bases
GCTCGGGCCGGTTGCGTCCGATGGCGGGCCGGCATGGCCCGCAGGCGCCAGCAGACGCAGTTGCCAGGATGCCATGCGTG
CAGGCGCGGGAGCGGTGCTT

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MKFFASCAKGLEYLLADELLALGASRATATISGVNVEGELRDAQRAVLWSRLASRVLWPLTEFDCPDEDALYAGVAELPW
DAHLSVGHTLSVDAHVSGTAITHARYAAQRIKDAVVDTMRRQGLERPSVDVENPDLRLNLSLRKGRATISVDLGGGPLHR
RGWRMAQNEAPLKENLAAAVLMRGGWPRAYADGGGLLDPMCGSGTLLIEGALMAADVAPGLQRYGSDVPSRWRGFDRDGW
QQLVGEARERDRVGRAALKQVIHGSDMDPHAIRAAKENAQVAGVAEAIWFGVREVGELQTPPQATGVVVCNPPYDERLAA
DAALYRRLGDTLQRVAPQWRASLLCGNAELAYATGLRAGKKYQLFNGAIECALIVCDPIAVPRRTPLAAPTALSEGAQMV
ANRLRKNLQKFKKWRAREGIECFRAYDADLPEYSAAIDVYQQADGDRRIFLHVQEYAAPATIPEADVRRRLNELLAAARE
VFEVPAERVALKSRERGKGGSKYGRFEQRNEIVHVREHGALLRVNLFDYLDTGLFLDHRPLRGTMAQQCRGRRFLNLFCY
TGVASVEAAVAGAASTTSVDLSATYLQWCADNLALNGLAGSKHKLVQADALAWLEAERAHFDVIFCDPPTFSNSARAEDF
DIQREHVRLLRAAVARLAPGGVLYFSNNFRRFKLDEEGVAEFAQCEEISPRTIDPDFERHARIHRAWRLTA

Sequences:

>Translated_711_residues
MKFFASCAKGLEYLLADELLALGASRATATISGVNVEGELRDAQRAVLWSRLASRVLWPLTEFDCPDEDALYAGVAELPW
DAHLSVGHTLSVDAHVSGTAITHARYAAQRIKDAVVDTMRRQGLERPSVDVENPDLRLNLSLRKGRATISVDLGGGPLHR
RGWRMAQNEAPLKENLAAAVLMRGGWPRAYADGGGLLDPMCGSGTLLIEGALMAADVAPGLQRYGSDVPSRWRGFDRDGW
QQLVGEARERDRVGRAALKQVIHGSDMDPHAIRAAKENAQVAGVAEAIWFGVREVGELQTPPQATGVVVCNPPYDERLAA
DAALYRRLGDTLQRVAPQWRASLLCGNAELAYATGLRAGKKYQLFNGAIECALIVCDPIAVPRRTPLAAPTALSEGAQMV
ANRLRKNLQKFKKWRAREGIECFRAYDADLPEYSAAIDVYQQADGDRRIFLHVQEYAAPATIPEADVRRRLNELLAAARE
VFEVPAERVALKSRERGKGGSKYGRFEQRNEIVHVREHGALLRVNLFDYLDTGLFLDHRPLRGTMAQQCRGRRFLNLFCY
TGVASVEAAVAGAASTTSVDLSATYLQWCADNLALNGLAGSKHKLVQADALAWLEAERAHFDVIFCDPPTFSNSARAEDF
DIQREHVRLLRAAVARLAPGGVLYFSNNFRRFKLDEEGVAEFAQCEEISPRTIDPDFERHARIHRAWRLTA
>Mature_711_residues
MKFFASCAKGLEYLLADELLALGASRATATISGVNVEGELRDAQRAVLWSRLASRVLWPLTEFDCPDEDALYAGVAELPW
DAHLSVGHTLSVDAHVSGTAITHARYAAQRIKDAVVDTMRRQGLERPSVDVENPDLRLNLSLRKGRATISVDLGGGPLHR
RGWRMAQNEAPLKENLAAAVLMRGGWPRAYADGGGLLDPMCGSGTLLIEGALMAADVAPGLQRYGSDVPSRWRGFDRDGW
QQLVGEARERDRVGRAALKQVIHGSDMDPHAIRAAKENAQVAGVAEAIWFGVREVGELQTPPQATGVVVCNPPYDERLAA
DAALYRRLGDTLQRVAPQWRASLLCGNAELAYATGLRAGKKYQLFNGAIECALIVCDPIAVPRRTPLAAPTALSEGAQMV
ANRLRKNLQKFKKWRAREGIECFRAYDADLPEYSAAIDVYQQADGDRRIFLHVQEYAAPATIPEADVRRRLNELLAAARE
VFEVPAERVALKSRERGKGGSKYGRFEQRNEIVHVREHGALLRVNLFDYLDTGLFLDHRPLRGTMAQQCRGRRFLNLFCY
TGVASVEAAVAGAASTTSVDLSATYLQWCADNLALNGLAGSKHKLVQADALAWLEAERAHFDVIFCDPPTFSNSARAEDF
DIQREHVRLLRAAVARLAPGGVLYFSNNFRRFKLDEEGVAEFAQCEEISPRTIDPDFERHARIHRAWRLTA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=718, Percent_Identity=44.9860724233983, Blast_Score=560, Evalue=1e-160,
Organism=Escherichia coli, GI145693111, Length=155, Percent_Identity=32.258064516129, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_XANAC (Q8PM40)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641926.1
- HSSP:   Q5SIT4
- ProteinModelPortal:   Q8PM40
- SMR:   Q8PM40
- GeneID:   1155665
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1594
- NMPDR:   fig|190486.1.peg.1570
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- ProtClustDB:   PRK11783
- BioCyc:   XAXO190486:XAC1594-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 78463; Mature: 78463

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFASCAKGLEYLLADELLALGASRATATISGVNVEGELRDAQRAVLWSRLASRVLWPL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
TEFDCPDEDALYAGVAELPWDAHLSVGHTLSVDAHVSGTAITHARYAAQRIKDAVVDTMR
CCCCCCCCCHHHHCHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
RQGLERPSVDVENPDLRLNLSLRKGRATISVDLGGGPLHRRGWRMAQNEAPLKENLAAAV
HCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
LMRGGWPRAYADGGGLLDPMCGSGTLLIEGALMAADVAPGLQRYGSDVPSRWRGFDRDGW
HHCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCHHHCCCCHHHH
QQLVGEARERDRVGRAALKQVIHGSDMDPHAIRAAKENAQVAGVAEAIWFGVREVGELQT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
PPQATGVVVCNPPYDERLAADAALYRRLGDTLQRVAPQWRASLLCGNAELAYATGLRAGK
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHEECCCCEEHHHCCCCCC
KYQLFNGAIECALIVCDPIAVPRRTPLAAPTALSEGAQMVANRLRKNLQKFKKWRAREGI
EEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ECFRAYDADLPEYSAAIDVYQQADGDRRIFLHVQEYAAPATIPEADVRRRLNELLAAARE
HHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHH
VFEVPAERVALKSRERGKGGSKYGRFEQRNEIVHVREHGALLRVNLFDYLDTGLFLDHRP
HHHCCHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEECCCCEEEEEHHHHHCCCCEECCCC
LRGTMAQQCRGRRFLNLFCYTGVASVEAAVAGAASTTSVDLSATYLQWCADNLALNGLAG
CCCHHHHHHCCCHHHHHHHHHCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHEEECCCC
SKHKLVQADALAWLEAERAHFDVIFCDPPTFSNSARAEDFDIQREHVRLLRAAVARLAPG
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GVLYFSNNFRRFKLDEEGVAEFAQCEEISPRTIDPDFERHARIHRAWRLTA
CEEEEECCCEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKFFASCAKGLEYLLADELLALGASRATATISGVNVEGELRDAQRAVLWSRLASRVLWPL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
TEFDCPDEDALYAGVAELPWDAHLSVGHTLSVDAHVSGTAITHARYAAQRIKDAVVDTMR
CCCCCCCCCHHHHCHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
RQGLERPSVDVENPDLRLNLSLRKGRATISVDLGGGPLHRRGWRMAQNEAPLKENLAAAV
HCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
LMRGGWPRAYADGGGLLDPMCGSGTLLIEGALMAADVAPGLQRYGSDVPSRWRGFDRDGW
HHCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCHHHCCCCHHHH
QQLVGEARERDRVGRAALKQVIHGSDMDPHAIRAAKENAQVAGVAEAIWFGVREVGELQT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
PPQATGVVVCNPPYDERLAADAALYRRLGDTLQRVAPQWRASLLCGNAELAYATGLRAGK
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHEECCCCEEHHHCCCCCC
KYQLFNGAIECALIVCDPIAVPRRTPLAAPTALSEGAQMVANRLRKNLQKFKKWRAREGI
EEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ECFRAYDADLPEYSAAIDVYQQADGDRRIFLHVQEYAAPATIPEADVRRRLNELLAAARE
HHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHH
VFEVPAERVALKSRERGKGGSKYGRFEQRNEIVHVREHGALLRVNLFDYLDTGLFLDHRP
HHHCCHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEECCCCEEEEEHHHHHCCCCEECCCC
LRGTMAQQCRGRRFLNLFCYTGVASVEAAVAGAASTTSVDLSATYLQWCADNLALNGLAG
CCCHHHHHHCCCHHHHHHHHHCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHEEECCCC
SKHKLVQADALAWLEAERAHFDVIFCDPPTFSNSARAEDFDIQREHVRLLRAAVARLAPG
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GVLYFSNNFRRFKLDEEGVAEFAQCEEISPRTIDPDFERHARIHRAWRLTA
CEEEEECCCEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217