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The map label for this gene is pmrB [H]

Identifier: 21242199

GI number: 21242199

Start: 1669134

End: 1670714

Strand: Direct

Name: pmrB [H]

Synonym: XAC1446

Alternate gene names: 21242199

Gene position: 1669134-1670714 (Clockwise)

Preceding gene: 21242198

Following gene: 77748587

Centisome position: 32.25

GC content: 63.06

Gene sequence:

>1581_bases
ATGTCTTCGCAAGCTCCCACCGCGTCCGGCGGCCCGGCGGCACCGGCGCCAGCGGCCGGTTTTCGCCCGGCCAGCGTGGC
GTTGTGCACCGTGGGCCTGGCCATGGCCTCGTTCATGCAGGTGCTGGACACCACCATCGCCAACGTCTCGCTGCCGACCA
TCGCCGGCAACCTCGGCGCCAGTTCGCAGCAGGCCACCTGGGTGATCACCTCGTTCGCCGTCAGCACCGCCATTGCGTTG
CCGCTGACCGGCTGGTTGAGTCGCCGGTTTGGCGAAACCAGGTTGTTCGTGTGGTCCACGCTGGCGTTCACCATTGCCTC
GCTGCTGTGCGGTCTGGCGCAGAGCATGGGGATGCTGGTGGTGGCGCGCGCGTTGCAGGGCTTTGTTGCCGGGCCGATGT
ATCCCATCACCCAGAGTCTGCTGGTGTCGATCTATCCGCGCGAAAAACGCGGGCAGGCGCTGGCGTTGCTGGCGATGATT
ACCGTGGTGGCCCCGATCGCCGGGCCGATTCTCGGCGGCTGGATCACCGACAACTACAGCTGGGAATGGATCTTCCTGAT
CAACGTGCCGCTGGGCATCATCGCCAGCAGTATCGTGGGTTCGCAGTTGCGGCATCGCCCCGAACAGCTCGAAAAGCCGC
GCATGGATTACATCGGCCTGATCCTGCTGGTGGTCGGCGTGGGCGCCCTGCAGCTGGTGCTGGATCTGGGCAATGACGAA
GACTGGTTCTCTTCGGACAAGATCGTGGTGTTGGCCTGCATTGCGGCAGTAGCGTTGGTGGTGTTCGTAATCTGGGAGTT
GACCGACAAGGATCCCATCGTCGATCTCAAGCTGTTCCGGCATCGCAATTTCCGCGCTGGCACGCTGGCAATGGTGGTCG
CATATGCCGCGTTCTTCAGCGTGAGCCTGCTGATCCCGCAATGGCTGCAACGCGATATGGGGTACACCGCGATCTGGGCC
GGCCTGGCCACCGCGCCGATCGGCATCCTGCCGGTGCTGATGACGCCATTCGTCGGCAAATACGCGCTGCGCTTCGACCT
GCGCATGCTGGCCACCATCGCCTTTATCTTCATGTCCTTCACCAGCTTCTTCCGCTCCAATTTCAACCTGCAGGTGGACT
TCGGCCATGTGGCCACGATCCAGTTGGTGATGGGTGTGGGCGTGGCATTGTTCTTCATGCCGGTGTTGCAGATCCTGTTG
TCGGATCTGGACGGTCGCGAGATTGCGGCCGGCTCCGGCCTGGCCACCTTCCTGCGCACGTTGGGCGGTAGCTTCGCCGC
ATCGTTGACCACGTATCTGTGGGCACGCCGTACCCAGGTACACCACGCGCACATCACCGAGCACATTTCGGTGTACACGC
CCGGCATGCAGGAGCAGGTCACTGCGATGGGGCAGGGCGACCTGCAACGCGGCGCGGCATCGCTCAATAACATGATCAAC
CACCAGGCTTCGCAGATGGGTTTCAACGACATCTTCTATCTGCTTGGATGGACCTTCCTGGCGATCATCTTCTTCCTGTG
GTTGGCAAAACCGCCGTTCGGTGCCGGGGCCGGGAGTGCGGCGGCGGCCGGGGGGCATTGA

Upstream 100 bases:

>100_bases
CGTGTATGCGCAGCAGCTCAAGGCTGCCGACGCCGACATCCAACGCATCATCCAGGACAACCTGCCGTCGCAGGCTGCCA
GCAAGGCGGGCTAAGCCACC

Downstream 100 bases:

>100_bases
GCGACGTTGAGGGTTGAACAACGAAAACCCCGCTCGAATGCGGGGTTTTCGTTGTGACGGATTTGACGAGCTTGGCCGAT
TGCCGCAGCGTCGAGCCGGT

Product: multidrug resistance membrane translocase

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSSQAPTASGGPAAPAPAAGFRPASVALCTVGLAMASFMQVLDTTIANVSLPTIAGNLGASSQQATWVITSFAVSTAIAL
PLTGWLSRRFGETRLFVWSTLAFTIASLLCGLAQSMGMLVVARALQGFVAGPMYPITQSLLVSIYPREKRGQALALLAMI
TVVAPIAGPILGGWITDNYSWEWIFLINVPLGIIASSIVGSQLRHRPEQLEKPRMDYIGLILLVVGVGALQLVLDLGNDE
DWFSSDKIVVLACIAAVALVVFVIWELTDKDPIVDLKLFRHRNFRAGTLAMVVAYAAFFSVSLLIPQWLQRDMGYTAIWA
GLATAPIGILPVLMTPFVGKYALRFDLRMLATIAFIFMSFTSFFRSNFNLQVDFGHVATIQLVMGVGVALFFMPVLQILL
SDLDGREIAAGSGLATFLRTLGGSFAASLTTYLWARRTQVHHAHITEHISVYTPGMQEQVTAMGQGDLQRGAASLNNMIN
HQASQMGFNDIFYLLGWTFLAIIFFLWLAKPPFGAGAGSAAAAGGH

Sequences:

>Translated_526_residues
MSSQAPTASGGPAAPAPAAGFRPASVALCTVGLAMASFMQVLDTTIANVSLPTIAGNLGASSQQATWVITSFAVSTAIAL
PLTGWLSRRFGETRLFVWSTLAFTIASLLCGLAQSMGMLVVARALQGFVAGPMYPITQSLLVSIYPREKRGQALALLAMI
TVVAPIAGPILGGWITDNYSWEWIFLINVPLGIIASSIVGSQLRHRPEQLEKPRMDYIGLILLVVGVGALQLVLDLGNDE
DWFSSDKIVVLACIAAVALVVFVIWELTDKDPIVDLKLFRHRNFRAGTLAMVVAYAAFFSVSLLIPQWLQRDMGYTAIWA
GLATAPIGILPVLMTPFVGKYALRFDLRMLATIAFIFMSFTSFFRSNFNLQVDFGHVATIQLVMGVGVALFFMPVLQILL
SDLDGREIAAGSGLATFLRTLGGSFAASLTTYLWARRTQVHHAHITEHISVYTPGMQEQVTAMGQGDLQRGAASLNNMIN
HQASQMGFNDIFYLLGWTFLAIIFFLWLAKPPFGAGAGSAAAAGGH
>Mature_525_residues
SSQAPTASGGPAAPAPAAGFRPASVALCTVGLAMASFMQVLDTTIANVSLPTIAGNLGASSQQATWVITSFAVSTAIALP
LTGWLSRRFGETRLFVWSTLAFTIASLLCGLAQSMGMLVVARALQGFVAGPMYPITQSLLVSIYPREKRGQALALLAMIT
VVAPIAGPILGGWITDNYSWEWIFLINVPLGIIASSIVGSQLRHRPEQLEKPRMDYIGLILLVVGVGALQLVLDLGNDED
WFSSDKIVVLACIAAVALVVFVIWELTDKDPIVDLKLFRHRNFRAGTLAMVVAYAAFFSVSLLIPQWLQRDMGYTAIWAG
LATAPIGILPVLMTPFVGKYALRFDLRMLATIAFIFMSFTSFFRSNFNLQVDFGHVATIQLVMGVGVALFFMPVLQILLS
DLDGREIAAGSGLATFLRTLGGSFAASLTTYLWARRTQVHHAHITEHISVYTPGMQEQVTAMGQGDLQRGAASLNNMINH
QASQMGFNDIFYLLGWTFLAIIFFLWLAKPPFGAGAGSAAAAGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=494, Percent_Identity=47.7732793522267, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI1788710, Length=506, Percent_Identity=43.8735177865613, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1790195, Length=322, Percent_Identity=33.5403726708075, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788392, Length=316, Percent_Identity=33.8607594936709, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87081983, Length=401, Percent_Identity=27.930174563591, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790146, Length=172, Percent_Identity=27.3255813953488, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789152, Length=186, Percent_Identity=25.8064516129032, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=27.2058823529412, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=280, Percent_Identity=25.7142857142857, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322958, Length=212, Percent_Identity=26.8867924528302, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319770, Length=328, Percent_Identity=26.5243902439024, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=26.1437908496732, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323520, Length=372, Percent_Identity=23.3870967741935, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56554; Mature: 56423

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQAPTASGGPAAPAPAAGFRPASVALCTVGLAMASFMQVLDTTIANVSLPTIAGNLGA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCC
SSQQATWVITSFAVSTAIALPLTGWLSRRFGETRLFVWSTLAFTIASLLCGLAQSMGMLV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARALQGFVAGPMYPITQSLLVSIYPREKRGQALALLAMITVVAPIAGPILGGWITDNYS
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCC
WEWIFLINVPLGIIASSIVGSQLRHRPEQLEKPRMDYIGLILLVVGVGALQLVLDLGNDE
EEEEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DWFSSDKIVVLACIAAVALVVFVIWELTDKDPIVDLKLFRHRNFRAGTLAMVVAYAAFFS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHH
VSLLIPQWLQRDMGYTAIWAGLATAPIGILPVLMTPFVGKYALRFDLRMLATIAFIFMSF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSFFRSNFNLQVDFGHVATIQLVMGVGVALFFMPVLQILLSDLDGREIAAGSGLATFLRT
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LGGSFAASLTTYLWARRTQVHHAHITEHISVYTPGMQEQVTAMGQGDLQRGAASLNNMIN
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHHHHHHHHHH
HQASQMGFNDIFYLLGWTFLAIIFFLWLAKPPFGAGAGSAAAAGGH
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSQAPTASGGPAAPAPAAGFRPASVALCTVGLAMASFMQVLDTTIANVSLPTIAGNLGA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCC
SSQQATWVITSFAVSTAIALPLTGWLSRRFGETRLFVWSTLAFTIASLLCGLAQSMGMLV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARALQGFVAGPMYPITQSLLVSIYPREKRGQALALLAMITVVAPIAGPILGGWITDNYS
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCC
WEWIFLINVPLGIIASSIVGSQLRHRPEQLEKPRMDYIGLILLVVGVGALQLVLDLGNDE
EEEEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DWFSSDKIVVLACIAAVALVVFVIWELTDKDPIVDLKLFRHRNFRAGTLAMVVAYAAFFS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHH
VSLLIPQWLQRDMGYTAIWAGLATAPIGILPVLMTPFVGKYALRFDLRMLATIAFIFMSF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSFFRSNFNLQVDFGHVATIQLVMGVGVALFFMPVLQILLSDLDGREIAAGSGLATFLRT
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LGGSFAASLTTYLWARRTQVHHAHITEHISVYTPGMQEQVTAMGQGDLQRGAASLNNMIN
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHHHHHHHHHH
HQASQMGFNDIFYLLGWTFLAIIFFLWLAKPPFGAGAGSAAAAGGH
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]