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The map label for this gene is phoB [H]

Identifier: 21241807

GI number: 21241807

Start: 1203405

End: 1204094

Strand: Reverse

Name: phoB [H]

Synonym: XAC1042

Alternate gene names: 21241807

Gene position: 1204094-1203405 (Counterclockwise)

Preceding gene: 21241808

Following gene: 21241806

Centisome position: 23.27

GC content: 65.22

Gene sequence:

>690_bases
GTGCAGAAACGCATTCTGATCGTCGATGACGAACCCGCGATCCGCGACATGGTGGCGTTCGCGCTGCGCAAAGGCGAGTT
CGAGCCCATCCATGCCGGCGATGCCCGCGAGGCGCAAACCGCCATCGCCGACCGCGTGCCGGACCTGATCCTGCTGGACT
GGATGTTGCCAGGCACCAGCGGGCTGGACCTGGCAAGGCGCTGGCGCAAGGAGCAGCTGACCCGCGAGATTCCGATCATC
ATGCTCACCGCGCGCGGCGAAGAAAACGACCGCGTCGGCGGCCTGGAAGCCGGCGTCGACGATTATGTGGTCAAGCCGTT
CTCGGCGCGCGAGCTGCTGGCACGCATCCGCGCGGTGATGCGGCGCACCCGCGAAGACGACGAAGACGGTAGCGTGGCGG
TAGGCAAGCTGCGCATCGACGGCGCCGCGCACCGGGTGTTCGCTGGCGATGCACCGGTGCCGATCGGCCCGACCGAATAT
CGGCTGCTGCACTTTTTCATGACACACCCCGAGCGCGTGTATACGCGTACCCAGCTACTGGACCATGTCTGGGGCGGCAG
CGTGTATGTGGAAGAGCGCACCATCGACGTGCATATCCGCCGCCTGCGCAAGACGCTGGAACCGTTCGGGCTGGAAAACA
TGGTGCAGACCGTACGTGGCTCCGGCTATCGTTTTTCTTCGGCGACCTGA

Upstream 100 bases:

>100_bases
CGGGTCGTTGCCGCCGCGCAGGCGCCGCCGACGATGTCACATTATAGTCACAAAACCGTAGTCTACTGGCGATCAACCAA
AGCCAGACGGTAAGCCCCGC

Downstream 100 bases:

>100_bases
CGTGCCGCAAAGCCACCCGGCCACAGTCCGGCCGGGTGGCTGCCTACCTGACGCTTCGTGGTAACGCCTTCCATGCCTCA
ACACATCCGTTCCGCCTGGC

Product: two-component system regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MQKRILIVDDEPAIRDMVAFALRKGEFEPIHAGDAREAQTAIADRVPDLILLDWMLPGTSGLDLARRWRKEQLTREIPII
MLTARGEENDRVGGLEAGVDDYVVKPFSARELLARIRAVMRRTREDDEDGSVAVGKLRIDGAAHRVFAGDAPVPIGPTEY
RLLHFFMTHPERVYTRTQLLDHVWGGSVYVEERTIDVHIRRLRKTLEPFGLENMVQTVRGSGYRFSSAT

Sequences:

>Translated_229_residues
MQKRILIVDDEPAIRDMVAFALRKGEFEPIHAGDAREAQTAIADRVPDLILLDWMLPGTSGLDLARRWRKEQLTREIPII
MLTARGEENDRVGGLEAGVDDYVVKPFSARELLARIRAVMRRTREDDEDGSVAVGKLRIDGAAHRVFAGDAPVPIGPTEY
RLLHFFMTHPERVYTRTQLLDHVWGGSVYVEERTIDVHIRRLRKTLEPFGLENMVQTVRGSGYRFSSAT
>Mature_229_residues
MQKRILIVDDEPAIRDMVAFALRKGEFEPIHAGDAREAQTAIADRVPDLILLDWMLPGTSGLDLARRWRKEQLTREIPII
MLTARGEENDRVGGLEAGVDDYVVKPFSARELLARIRAVMRRTREDDEDGSVAVGKLRIDGAAHRVFAGDAPVPIGPTEY
RLLHFFMTHPERVYTRTQLLDHVWGGSVYVEERTIDVHIRRLRKTLEPFGLENMVQTVRGSGYRFSSAT

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=58.5903083700441, Blast_Score=283, Evalue=8e-78,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=38.9380530973451, Blast_Score=150, Evalue=5e-38,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=37.5545851528384, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1790860, Length=230, Percent_Identity=35.6521739130435, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=33.9285714285714, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=31.0810810810811, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=35.2678571428571, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=33.6363636363636, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=32.034632034632, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI2367329, Length=240, Percent_Identity=35.4166666666667, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=32.2727272727273, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=31.8181818181818, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787229, Length=235, Percent_Identity=30.2127659574468, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI145693140, Length=238, Percent_Identity=26.890756302521, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1788550, Length=110, Percent_Identity=39.0909090909091, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26003; Mature: 26003

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKRILIVDDEPAIRDMVAFALRKGEFEPIHAGDAREAQTAIADRVPDLILLDWMLPGTS
CCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCC
GLDLARRWRKEQLTREIPIIMLTARGEENDRVGGLEAGVDDYVVKPFSARELLARIRAVM
CHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHH
RRTREDDEDGSVAVGKLRIDGAAHRVFAGDAPVPIGPTEYRLLHFFMTHPERVYTRTQLL
HHHCCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
DHVWGGSVYVEERTIDVHIRRLRKTLEPFGLENMVQTVRGSGYRFSSAT
HHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure
MQKRILIVDDEPAIRDMVAFALRKGEFEPIHAGDAREAQTAIADRVPDLILLDWMLPGTS
CCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCC
GLDLARRWRKEQLTREIPIIMLTARGEENDRVGGLEAGVDDYVVKPFSARELLARIRAVM
CHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHH
RRTREDDEDGSVAVGKLRIDGAAHRVFAGDAPVPIGPTEYRLLHFFMTHPERVYTRTQLL
HHHCCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
DHVWGGSVYVEERTIDVHIRRLRKTLEPFGLENMVQTVRGSGYRFSSAT
HHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]