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The map label for this gene is trpE [H]

Identifier: 21241249

GI number: 21241249

Start: 566310

End: 567785

Strand: Direct

Name: trpE [H]

Synonym: XAC0476

Alternate gene names: 21241249

Gene position: 566310-567785 (Clockwise)

Preceding gene: 21241248

Following gene: 77748529

Centisome position: 10.94

GC content: 65.24

Gene sequence:

>1476_bases
TTGATCACTGCAGAGCAGTTCCAGCGCCAGGCCGCTGAAGGTCACACCCGTATTCCCGTTGTCCGCGAAGTGTTGTCCGA
CCTGGACACGCCGCTCTCGGTCTACCTGAAGCTCGCCGACGGCGCCTATACCTATCTGTTCGAATCGGTCGAAGGCGGGG
AGCGCTTCGGGCGCTACTCCATCATCGGCCTGCCGGCGCGGCGCGTGTACAGCTTTCGGGGCCACACGCTGGAGGTCAGC
GAACACGGCGAAGTCGTCGAGACCCGTGAGGTCGCCGACCCGCTGGCCGAAGTCGATGCGTTGCGGGCCGAACATTCGGT
GCCGCAGCTGGATGGGCTGCCCGGCTTCACCGGCGGGCTGGTCGGCTGGTTCGGTTTCGAGTGCATCCAATACATCGAGC
CGCGCCTGGGGAGTGGCGACAAGCCCGACGAACTCGGCACGCCGGACATCCTGCTGATGCTCTCCGAAGAGCTGGCGGTG
TTCGACAATCTCAAGGGCCGTCTGTACCTGATCGTGCATGCCGACCCGCGGCAGCCGCAAGCCTATGTGCGCGCCAATCG
GCGCCTGGACGAGCTGGCGCATCGCCTGCGTCAGGGCGGCGCCGGTTACCCGCAGGCGCAGATTTCCGATGCGATCGACG
AAGCGGACTTCCATTCCTCTTTTACCCGCGAGGAATATCACGCCGTGGTGCGCAAGGCGCAGGAATACGTGCGTGCCGGC
GATATCTTCCAGGTAGTGCCATCGCAACGCTTGCGCGTGCCGTTCCGCGCGCGCCCGGTGGATGTGTATCGCGCGTTGCG
TGCACTGAATCCGTCGCCGTACATGTATTTTCTCGATGTCGGCGGTACCCAGGTGGTTGGTTCGTCGCCAGAAATTCTGG
CGCGCCTGCGCGATGGCGTGGTCACTGTGCGCCCGATCGCCGGCACCCGCCCGCGCGGCGCCACGCCCGAACTGGACAAG
GCACTGGAAGCCGAACTGCTGGCCGACCCGAAGGAACGCGCCGAGCACGTGATGCTGATCGATCTGGGTCGCAACGATGT
CGGCCGCGTGGCCGAACCGGGCACGGTCAAGGTCGGCGAGCAGTTCGTGATCGAACGCTATAGCCATGTCATGCACATCG
TCAGCGAGGTGACCGGCACCCTGAAAGCCGGCCTGAATTATAGCGACGTGCTGCGCGCCACCTTCCCGGCCGGCACTGTC
AGCGGCGCGCCGAAGATCCGCGCGCTGGAAATCATCCGCGAGCTGGAGCCGGTCAAACGCAACGTGTATTCCGGCGCGGT
CGGCTATATCGGCTGGCATGGCGATGCCGATACCGCCATCGCCATCCGCACCGCCGTCATCCAGGACGGTTACCTGTATG
TGCAGGCCGGCGGCGGCGTGGTCTACGACTCCGACCCCGACCTGGAGTGGCAGGAAACCATGAACAAGGGGCGTGCGCTG
TTTCGCGCAGTTGCCCAGGCGGCGAAGGGCCTGTGA

Upstream 100 bases:

>100_bases
CTGACGACTCCACGCTCCTTCTCTTCCGCTCGCCGCTGGTGGCGATGGTGGCCCGTTGCCGTCGTCCGCCCCGCCACAGA
GTCCCGGAAGGAAAGTCGTC

Downstream 100 bases:

>100_bases
TGGACGGGCAGGGTAGCGCTACGCGCATCAGCTTTCGCAACGACTACAGCGAAGGCGCGCATCCGCGGCTGTTGCAGGCG
TTGGCGCAGGCCAGCGCCGA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MITAEQFQRQAAEGHTRIPVVREVLSDLDTPLSVYLKLADGAYTYLFESVEGGERFGRYSIIGLPARRVYSFRGHTLEVS
EHGEVVETREVADPLAEVDALRAEHSVPQLDGLPGFTGGLVGWFGFECIQYIEPRLGSGDKPDELGTPDILLMLSEELAV
FDNLKGRLYLIVHADPRQPQAYVRANRRLDELAHRLRQGGAGYPQAQISDAIDEADFHSSFTREEYHAVVRKAQEYVRAG
DIFQVVPSQRLRVPFRARPVDVYRALRALNPSPYMYFLDVGGTQVVGSSPEILARLRDGVVTVRPIAGTRPRGATPELDK
ALEAELLADPKERAEHVMLIDLGRNDVGRVAEPGTVKVGEQFVIERYSHVMHIVSEVTGTLKAGLNYSDVLRATFPAGTV
SGAPKIRALEIIRELEPVKRNVYSGAVGYIGWHGDADTAIAIRTAVIQDGYLYVQAGGGVVYDSDPDLEWQETMNKGRAL
FRAVAQAAKGL

Sequences:

>Translated_491_residues
MITAEQFQRQAAEGHTRIPVVREVLSDLDTPLSVYLKLADGAYTYLFESVEGGERFGRYSIIGLPARRVYSFRGHTLEVS
EHGEVVETREVADPLAEVDALRAEHSVPQLDGLPGFTGGLVGWFGFECIQYIEPRLGSGDKPDELGTPDILLMLSEELAV
FDNLKGRLYLIVHADPRQPQAYVRANRRLDELAHRLRQGGAGYPQAQISDAIDEADFHSSFTREEYHAVVRKAQEYVRAG
DIFQVVPSQRLRVPFRARPVDVYRALRALNPSPYMYFLDVGGTQVVGSSPEILARLRDGVVTVRPIAGTRPRGATPELDK
ALEAELLADPKERAEHVMLIDLGRNDVGRVAEPGTVKVGEQFVIERYSHVMHIVSEVTGTLKAGLNYSDVLRATFPAGTV
SGAPKIRALEIIRELEPVKRNVYSGAVGYIGWHGDADTAIAIRTAVIQDGYLYVQAGGGVVYDSDPDLEWQETMNKGRAL
FRAVAQAAKGL
>Mature_491_residues
MITAEQFQRQAAEGHTRIPVVREVLSDLDTPLSVYLKLADGAYTYLFESVEGGERFGRYSIIGLPARRVYSFRGHTLEVS
EHGEVVETREVADPLAEVDALRAEHSVPQLDGLPGFTGGLVGWFGFECIQYIEPRLGSGDKPDELGTPDILLMLSEELAV
FDNLKGRLYLIVHADPRQPQAYVRANRRLDELAHRLRQGGAGYPQAQISDAIDEADFHSSFTREEYHAVVRKAQEYVRAG
DIFQVVPSQRLRVPFRARPVDVYRALRALNPSPYMYFLDVGGTQVVGSSPEILARLRDGVVTVRPIAGTRPRGATPELDK
ALEAELLADPKERAEHVMLIDLGRNDVGRVAEPGTVKVGEQFVIERYSHVMHIVSEVTGTLKAGLNYSDVLRATFPAGTV
SGAPKIRALEIIRELEPVKRNVYSGAVGYIGWHGDADTAIAIRTAVIQDGYLYVQAGGGVVYDSDPDLEWQETMNKGRAL
FRAVAQAAKGL

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=394, Percent_Identity=39.0862944162437, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1788114, Length=474, Percent_Identity=32.0675105485232, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI87082077, Length=280, Percent_Identity=27.5, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1786809, Length=209, Percent_Identity=27.7511961722488, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=501, Percent_Identity=38.3233532934132, Blast_Score=321, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6324361, Length=392, Percent_Identity=26.7857142857143, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 54175; Mature: 54175

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITAEQFQRQAAEGHTRIPVVREVLSDLDTPLSVYLKLADGAYTYLFESVEGGERFGRYS
CCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHEEEECCCCCEEHHHHHHCCHHHCCCEE
IIGLPARRVYSFRGHTLEVSEHGEVVETREVADPLAEVDALRAEHSVPQLDGLPGFTGGL
EEECCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
VGWFGFECIQYIEPRLGSGDKPDELGTPDILLMLSEELAVFDNLKGRLYLIVHADPRQPQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCCH
AYVRANRRLDELAHRLRQGGAGYPQAQISDAIDEADFHSSFTREEYHAVVRKAQEYVRAG
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DIFQVVPSQRLRVPFRARPVDVYRALRALNPSPYMYFLDVGGTQVVGSSPEILARLRDGV
CHHEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEECCCHHHHHHHHCCC
VTVRPIAGTRPRGATPELDKALEAELLADPKERAEHVMLIDLGRNDVGRVAEPGTVKVGE
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHCCEEEEEECCCCCCCCCCCCCCEEHHH
QFVIERYSHVMHIVSEVTGTLKAGLNYSDVLRATFPAGTVSGAPKIRALEIIRELEPVKR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
NVYSGAVGYIGWHGDADTAIAIRTAVIQDGYLYVQAGGGVVYDSDPDLEWQETMNKGRAL
HHHCCCCEEEEECCCCCCEEEEEEEEEECCEEEEEECCEEEECCCCCCCHHHHHHHHHHH
FRAVAQAAKGL
HHHHHHHHCCC
>Mature Secondary Structure
MITAEQFQRQAAEGHTRIPVVREVLSDLDTPLSVYLKLADGAYTYLFESVEGGERFGRYS
CCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHEEEECCCCCEEHHHHHHCCHHHCCCEE
IIGLPARRVYSFRGHTLEVSEHGEVVETREVADPLAEVDALRAEHSVPQLDGLPGFTGGL
EEECCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
VGWFGFECIQYIEPRLGSGDKPDELGTPDILLMLSEELAVFDNLKGRLYLIVHADPRQPQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCCH
AYVRANRRLDELAHRLRQGGAGYPQAQISDAIDEADFHSSFTREEYHAVVRKAQEYVRAG
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DIFQVVPSQRLRVPFRARPVDVYRALRALNPSPYMYFLDVGGTQVVGSSPEILARLRDGV
CHHEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEECCCHHHHHHHHCCC
VTVRPIAGTRPRGATPELDKALEAELLADPKERAEHVMLIDLGRNDVGRVAEPGTVKVGE
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHCCEEEEEECCCCCCCCCCCCCCEEHHH
QFVIERYSHVMHIVSEVTGTLKAGLNYSDVLRATFPAGTVSGAPKIRALEIIRELEPVKR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
NVYSGAVGYIGWHGDADTAIAIRTAVIQDGYLYVQAGGGVVYDSDPDLEWQETMNKGRAL
HHHCCCCEEEEECCCCCCEEEEEEEEEECCEEEEEECCEEEECCCCCCCHHHHHHHHHHH
FRAVAQAAKGL
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1987141 [H]