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The map label for this gene is yegS [C]

Identifier: 21241248

GI number: 21241248

Start: 564812

End: 565741

Strand: Direct

Name: yegS [C]

Synonym: XAC0475

Alternate gene names: 21241248

Gene position: 564812-565741 (Clockwise)

Preceding gene: 21241247

Following gene: 21241249

Centisome position: 10.91

GC content: 69.46

Gene sequence:

>930_bases
ATGGCCCCGTCCCACTGGCGTCTGATCCTCAACGGCAAGTCCACCGATAATCTGGACCTGCGTGAGGCAGTGGGAACGCT
GCGCAAGCGCGGCATCCAGCTCGATGTGCGGGTCACCTGGGAAGACGGCGATGCCGAGCGCTACGTGGGCGAGGCAGTGG
CCGATGGCGTGCATACGGTGGTGGCCGCCGGTGGCGACGGCACTCTGAGCGAAGTGGCGGCCGCCCTGGCGCATCACGAG
GGCGATGCGGCCACGCTGCCGTCGCTGGGCCTGGTGCCGCTGGGCACCGCCAACGACTTCGCCACCGCCGCCACTCTGCC
GATCGCCCCGCTGGAGGCGTTGACCTTGATCGCCGAGCGCGTCGCGCAGCCGGTCGACTTGCTGCGGATCGACGCCGACC
ACGGCCCGCACTGGTGCGCCAATGTGGCCAGCGGCGGCTTCGGCACCCAGGTCACCGTGGAAACCGACGAAGGCCTGAAG
AAGATGCTCGGCGGTCTGGCCTATCTCATCACCGGCATGTCGCGGCTGGGCCGCATCGACCCGATCAGCGCCCGCTTCAG
CGGCCCGGAGTTCAGCTGGGAAGGCGAATTCATCGCGCTCGGGCTGGGGAACGGGCGCCAGGCCGGTGGCGGCCAGGCGC
TGTGCCCGGAGGCATTGATCGACGACGGCCTGCTGGATGTCACCATCGTCCCCGACCTTGACGGCGAAGTGGCCGCCACG
CTCGGCACCCTGGTCACCGGCGGCAAACAGGCCGCGCTCGAACGGGTGGCCGTGCGCGCGCGCGTGCCGTGGCTGGACAT
CGTCTCCCAGCAACCGCTGACCTTGAACCTGGACGGCGAACCGGAAACCTCCCGGCACTTCCGCATCGAATGCGTCCCGG
CGCGGCTGCGGATGCATTTGCCGGGTGAGTGCCCTTTGCTTGGTGGGTGA

Upstream 100 bases:

>100_bases
TGACACCGCAATGACAGTGTGCCGGCGGCGCCTATTCACGATCTGCCCATTGGCCCGCTGCTAGCCTGCGGCCATTACCT
TCACAGCATCGGCAGCGCGC

Downstream 100 bases:

>100_bases
GAATCGGGAATCGGGAATCGGGAATCGGGAATCGGGAATCGGGAATCGGAAAAGCGTGGCAGGCATTTCGGGTGAGCCAG
GCAATCGCCTCGCTTTACGC

Product: lipid kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MAPSHWRLILNGKSTDNLDLREAVGTLRKRGIQLDVRVTWEDGDAERYVGEAVADGVHTVVAAGGDGTLSEVAAALAHHE
GDAATLPSLGLVPLGTANDFATAATLPIAPLEALTLIAERVAQPVDLLRIDADHGPHWCANVASGGFGTQVTVETDEGLK
KMLGGLAYLITGMSRLGRIDPISARFSGPEFSWEGEFIALGLGNGRQAGGGQALCPEALIDDGLLDVTIVPDLDGEVAAT
LGTLVTGGKQAALERVAVRARVPWLDIVSQQPLTLNLDGEPETSRHFRIECVPARLRMHLPGECPLLGG

Sequences:

>Translated_309_residues
MAPSHWRLILNGKSTDNLDLREAVGTLRKRGIQLDVRVTWEDGDAERYVGEAVADGVHTVVAAGGDGTLSEVAAALAHHE
GDAATLPSLGLVPLGTANDFATAATLPIAPLEALTLIAERVAQPVDLLRIDADHGPHWCANVASGGFGTQVTVETDEGLK
KMLGGLAYLITGMSRLGRIDPISARFSGPEFSWEGEFIALGLGNGRQAGGGQALCPEALIDDGLLDVTIVPDLDGEVAAT
LGTLVTGGKQAALERVAVRARVPWLDIVSQQPLTLNLDGEPETSRHFRIECVPARLRMHLPGECPLLGG
>Mature_308_residues
APSHWRLILNGKSTDNLDLREAVGTLRKRGIQLDVRVTWEDGDAERYVGEAVADGVHTVVAAGGDGTLSEVAAALAHHEG
DAATLPSLGLVPLGTANDFATAATLPIAPLEALTLIAERVAQPVDLLRIDADHGPHWCANVASGGFGTQVTVETDEGLKK
MLGGLAYLITGMSRLGRIDPISARFSGPEFSWEGEFIALGLGNGRQAGGGQALCPEALIDDGLLDVTIVPDLDGEVAATL
GTLVTGGKQAALERVAVRARVPWLDIVSQQPLTLNLDGEPETSRHFRIECVPARLRMHLPGECPLLGG

Specific function: Probably phosphorylates lipids; the in vivo substrate is unknown

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain

Homologues:

Organism=Escherichia coli, GI1788402, Length=301, Percent_Identity=47.5083056478405, Blast_Score=255, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YEGS_XANAC (Q8PQ53)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_640830.1
- ProteinModelPortal:   Q8PQ53
- SMR:   Q8PQ53
- GeneID:   1154546
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0475
- NMPDR:   fig|190486.1.peg.474
- HOGENOM:   HBG698136
- OMA:   FRIECVP
- ProtClustDB:   PRK13054
- BioCyc:   XAXO190486:XAC0475-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01377
- InterPro:   IPR005218
- InterPro:   IPR001206
- InterPro:   IPR022433
- InterPro:   IPR022862
- SMART:   SM00046
- TIGRFAMs:   TIGR00147
- TIGRFAMs:   TIGR03702

Pfam domain/function: PF00781 DAGK_cat

EC number: NA

Molecular weight: Translated: 32429; Mature: 32298

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50146 DAGK

Important sites: ACT_SITE 280-280 BINDING 39-39 BINDING 96-96

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPSHWRLILNGKSTDNLDLREAVGTLRKRGIQLDVRVTWEDGDAERYVGEAVADGVHTV
CCCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHCCCEEE
VAAGGDGTLSEVAAALAHHEGDAATLPSLGLVPLGTANDFATAATLPIAPLEALTLIAER
EEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHCCCCCCHHHHHHHHHHH
VAQPVDLLRIDADHGPHWCANVASGGFGTQVTVETDEGLKKMLGGLAYLITGMSRLGRID
HCCCEEEEEEECCCCCCHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCC
PISARFSGPEFSWEGEFIALGLGNGRQAGGGQALCPEALIDDGLLDVTIVPDLDGEVAAT
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCHHHHH
LGTLVTGGKQAALERVAVRARVPWLDIVSQQPLTLNLDGEPETSRHFRIECVPARLRMHL
HHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCCCCCCEEEEEEEEEEEEEEC
PGECPLLGG
CCCCCCCCC
>Mature Secondary Structure 
APSHWRLILNGKSTDNLDLREAVGTLRKRGIQLDVRVTWEDGDAERYVGEAVADGVHTV
CCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHCCCEEE
VAAGGDGTLSEVAAALAHHEGDAATLPSLGLVPLGTANDFATAATLPIAPLEALTLIAER
EEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHCCCCCCHHHHHHHHHHH
VAQPVDLLRIDADHGPHWCANVASGGFGTQVTVETDEGLKKMLGGLAYLITGMSRLGRID
HCCCEEEEEEECCCCCCHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCC
PISARFSGPEFSWEGEFIALGLGNGRQAGGGQALCPEALIDDGLLDVTIVPDLDGEVAAT
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCHHHHH
LGTLVTGGKQAALERVAVRARVPWLDIVSQQPLTLNLDGEPETSRHFRIECVPARLRMHL
HHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCCCCCCEEEEEEEEEEEEEEC
PGECPLLGG
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217