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The map label for this gene is bioB

Identifier: 21241162

GI number: 21241162

Start: 458779

End: 459813

Strand: Reverse

Name: bioB

Synonym: XAC0388

Alternate gene names: 21241162

Gene position: 459813-458779 (Counterclockwise)

Preceding gene: 21241164

Following gene: 21241161

Centisome position: 8.88

GC content: 66.86

Gene sequence:

>1035_bases
ATGTCTGTTGTCCTCCGCCATGACTGGGATCGCAAAGAGCTGCAGGCGCTGTTCGATCTGCCGTTTCCGGAGTTGCTGCA
CCGCGCGGCCAGCGTGCATCGCGCGCACTTCGATCCGGCGCAAGTGCAGGTGTCCACCTTGCTCTCGGTCAAGACCGGCG
GCTGCCCGGAAGACTGCGCGTATTGCCCGCAGGCGCAGCGCTACGACACAGGCGTGAGCGCGCAGAAGCTCATGGAGACC
GAAGAGGTCGTCGCCAAGGCGCGCCAGGCCAAGGCCGCCGGCGCCTCGCGCTTCTGCATGGGCGCCGCCTGGCGCTCGCC
CAAGGAGCGCGACATTCCCAAGGTGGCCGCCATGATCCGCGAGGTGAAGGCCATGGGCCTGGAAACCTGCGCCACGCTCG
GCATGCTCGACGCCGGCCAGGCGCGTGCGCTCAAGGACGCTGGGCTGGACTACTACAACCACAATCTGGACACCGCGCCG
GATTACTACGACTCGATCATCCACACCCGCCAGTACCAAGACCGCCTGAACACGCTGGAGCATGTGCGCGATGTCGGCCT
GAAGACCTGCTGCGGCGGCATCGTCGGCATGGGCGAAACCCGCGAACACCGCATCGGTCTGCTGCTGGCGCTGGCCACGC
TGCCGGCGCATCCGGATTCGGTGCCGATCAACCAGCTGGTGCAGGTACCCGGTACTCCGCTGCACGGCACCCAGCAGCTG
GATCCGTTCGAGTTCGTGCGCATGATCGCCGTTGCCCGTATCGCCATGCCGAAATCGATGGTGCGCCTGTCCGCCGGCCG
CGAGACGATGAGCGACGAACTGCAGGCGCTGTGCTTCCTGGCCGGTGCCAATTCGATTTTCTACGGCGAAAAACTGCTCA
CCACCGGCAACCCGGACACCGAGCGCGACCAGGCCCTGTTCCAGCGCCTGGGCCTGCGCCCGATGCAGGTCACCGTCGAT
GCGGCCGAGCACGATCACCCCGGCACCGTCCATGCGGAGATCACCCGTAGCGCGGCCTGCGAGCACGCCGCCTGA

Upstream 100 bases:

>100_bases
CGGCCATCTGTAAACCGATCGGACCTGATTGAAGTTGACAGTCTCTTGCATGCTCACCAGACTGCCTGGCTTCCAAGCCG
CTGACTGTCAGGAAACTCCG

Downstream 100 bases:

>100_bases
CCCGGCACCGGGCACCGGTCGTCGGTGGCGGCTGGGCGCCCTGAGCCCGGCACGCTACGCTAGCCGGCCTTCCCTGCCGT
TGAACGCCCCCATGGCTCGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSVVLRHDWDRKELQALFDLPFPELLHRAASVHRAHFDPAQVQVSTLLSVKTGGCPEDCAYCPQAQRYDTGVSAQKLMET
EEVVAKARQAKAAGASRFCMGAAWRSPKERDIPKVAAMIREVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAP
DYYDSIIHTRQYQDRLNTLEHVRDVGLKTCCGGIVGMGETREHRIGLLLALATLPAHPDSVPINQLVQVPGTPLHGTQQL
DPFEFVRMIAVARIAMPKSMVRLSAGRETMSDELQALCFLAGANSIFYGEKLLTTGNPDTERDQALFQRLGLRPMQVTVD
AAEHDHPGTVHAEITRSAACEHAA

Sequences:

>Translated_344_residues
MSVVLRHDWDRKELQALFDLPFPELLHRAASVHRAHFDPAQVQVSTLLSVKTGGCPEDCAYCPQAQRYDTGVSAQKLMET
EEVVAKARQAKAAGASRFCMGAAWRSPKERDIPKVAAMIREVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAP
DYYDSIIHTRQYQDRLNTLEHVRDVGLKTCCGGIVGMGETREHRIGLLLALATLPAHPDSVPINQLVQVPGTPLHGTQQL
DPFEFVRMIAVARIAMPKSMVRLSAGRETMSDELQALCFLAGANSIFYGEKLLTTGNPDTERDQALFQRLGLRPMQVTVD
AAEHDHPGTVHAEITRSAACEHAA
>Mature_343_residues
SVVLRHDWDRKELQALFDLPFPELLHRAASVHRAHFDPAQVQVSTLLSVKTGGCPEDCAYCPQAQRYDTGVSAQKLMETE
EVVAKARQAKAAGASRFCMGAAWRSPKERDIPKVAAMIREVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAPD
YYDSIIHTRQYQDRLNTLEHVRDVGLKTCCGGIVGMGETREHRIGLLLALATLPAHPDSVPINQLVQVPGTPLHGTQQLD
PFEFVRMIAVARIAMPKSMVRLSAGRETMSDELQALCFLAGANSIFYGEKLLTTGNPDTERDQALFQRLGLRPMQVTVDA
AEHDHPGTVHAEITRSAACEHAA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=321, Percent_Identity=60.7476635514019, Blast_Score=421, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=45.9119496855346, Blast_Score=325, Evalue=5e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_XANAC (Q8PQD7)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_640744.1
- HSSP:   P12996
- ProteinModelPortal:   Q8PQD7
- SMR:   Q8PQD7
- GeneID:   1154459
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0388
- NMPDR:   fig|190486.1.peg.388
- HOGENOM:   HBG405569
- OMA:   LEICSGG
- ProtClustDB:   CLSK2300892
- BioCyc:   XAXO190486:XAC0388-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37780; Mature: 37648

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVLRHDWDRKELQALFDLPFPELLHRAASVHRAHFDPAQVQVSTLLSVKTGGCPEDCA
CCEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHH
YCPQAQRYDTGVSAQKLMETEEVVAKARQAKAAGASRFCMGAAWRSPKERDIPKVAAMIR
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHH
EVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAPDYYDSIIHTRQYQDRLNTLE
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HVRDVGLKTCCGGIVGMGETREHRIGLLLALATLPAHPDSVPINQLVQVPGTPLHGTQQL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCC
DPFEFVRMIAVARIAMPKSMVRLSAGRETMSDELQALCFLAGANSIFYGEKLLTTGNPDT
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEECCEEEECCCCCC
ERDQALFQRLGLRPMQVTVDAAEHDHPGTVHAEITRSAACEHAA
HHHHHHHHHHCCCCEEEEEECCCCCCCCCEEHHHHHHHHHCCCC
>Mature Secondary Structure 
SVVLRHDWDRKELQALFDLPFPELLHRAASVHRAHFDPAQVQVSTLLSVKTGGCPEDCA
CEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHH
YCPQAQRYDTGVSAQKLMETEEVVAKARQAKAAGASRFCMGAAWRSPKERDIPKVAAMIR
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHH
EVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAPDYYDSIIHTRQYQDRLNTLE
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HVRDVGLKTCCGGIVGMGETREHRIGLLLALATLPAHPDSVPINQLVQVPGTPLHGTQQL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCC
DPFEFVRMIAVARIAMPKSMVRLSAGRETMSDELQALCFLAGANSIFYGEKLLTTGNPDT
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEECCEEEECCCCCC
ERDQALFQRLGLRPMQVTVDAAEHDHPGTVHAEITRSAACEHAA
HHHHHHHHHHCCCCEEEEEECCCCCCCCCEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217