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The map label for this gene is bioF

Identifier: 21241161

GI number: 21241161

Start: 457482

End: 458687

Strand: Reverse

Name: bioF

Synonym: XAC0387

Alternate gene names: 21241161

Gene position: 458687-457482 (Counterclockwise)

Preceding gene: 21241162

Following gene: 21241160

Centisome position: 8.86

GC content: 67.41

Gene sequence:

>1206_bases
ATGGCTCGCCCCGATCTGCACGAACGGATTTCGTCGCTGCGTAAATTGCGTGTGGCCCAGGAACGGGTGCGGGTGCGACG
GCAGGTGGGCCGGCGCGATGGCGTGCGGCTGGAGATCGACGGCCGTTGGCTGACCGGTTTCTGTTCCAACGACTACCTGG
GCTTGTCGCAACAGTTCGAAGTGGTCGCCGCGCTGCAGGATGCCGCTGCCCGCGATGGTGCCGGCGCCACCGCCTCGCAT
CTGATCTGCGGGCACCACACCGCGCACGAAACATTGGAACGCGAAATCGCCGAATGGCTGGGCTATCCGTCGGCGTTGCT
GTTCGGCAGCGGCTTCATCGCCAACCTGGCCGTGCAACAGGCGTTGCTCAGCGAAGAAGACGATGTCTGCGTGCAGGACC
GGCTCAATCACGCCAGCCTGCTCGATGCCACGCGGCTGGCCGGTTGCCGGTTGCGGCGCTATCCGCACCTGGATGTGGAA
GGCGCGATGCGCCAGCTCAAGGGCGCGCCCGAAGGCGCGGCGATGCTGGCCACAGACGGCGTTTTCAGCATGGACGGCGA
TGTCGCGCCGCTGCGCGCCTTGAGCCTGGTGGCACGCATGCAGCAAGCGCTGTTCTATGTGGACGACGCGCACGGTGTTG
GCGTGCTCGGTCCGCAAGGGCGCGGTTGCGTCGCCGATGCCGGGTTGGGCGTGGCCGAAGTGCCGTTGCAGTTGGTCACC
CTGGGCAAGGCGCTCGGTGGCTACGGCGCGGTGGTGGTGGGCGAAGAGGCGTTGGTGCGCCATCTGGCCGAAACTGCGCG
CCCCTACATCTACACCACCGCATTGCCGCCGGCGCAGGTCGCGGCCACCCTGGCGGCCGTGCGCCTGGCGCGTCGCGACG
ATTGGCGGCGTGCGCGCCTGGTCGAATTGATCGGTGCGTTCCGCGATGGCGCGCGCAAGCACGGCTTCGAACTGATGGCC
TCCGACACGCCGATCCAGCCACTGCTATGCGGCGAGGAAGCAACCGTGATGGCGATGTCGGCTGCGCTCGAACACGCAGG
TTTCATGGTTGGTGCCATTCGTCCGCCCACCGTGCCCGAAGGCAAGGCGCGCTTGCGCGTCACCTTGTCTGCGCTGCATA
CGCAGCAGCAGGTGCAGGCACTGATCGAGGCCATCGTGCAGGCGCGCGATGTGGTCAGTCGGCAGCCGCTGCGTGCCTCT
GCCTGA

Upstream 100 bases:

>100_bases
GCCGCCTGACCCGGCACCGGGCACCGGTCGTCGGTGGCGGCTGGGCGCCCTGAGCCCGGCACGCTACGCTAGCCGGCCTT
CCCTGCCGTTGAACGCCCCC

Downstream 100 bases:

>100_bases
GGCGAGTGGCAGCGCGTGGCTGGCCTGCACGCAGACACGCGCGGGCCACTCAGCCAATGCAGCGATCGCACGACGCACCT
GCGTGGCAGGCGACCACGGC

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MARPDLHERISSLRKLRVAQERVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFEVVAALQDAAARDGAGATASH
LICGHHTAHETLEREIAEWLGYPSALLFGSGFIANLAVQQALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVE
GAMRQLKGAPEGAAMLATDGVFSMDGDVAPLRALSLVARMQQALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
LGKALGGYGAVVVGEEALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRARLVELIGAFRDGARKHGFELMA
SDTPIQPLLCGEEATVMAMSAALEHAGFMVGAIRPPTVPEGKARLRVTLSALHTQQQVQALIEAIVQARDVVSRQPLRAS
A

Sequences:

>Translated_401_residues
MARPDLHERISSLRKLRVAQERVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFEVVAALQDAAARDGAGATASH
LICGHHTAHETLEREIAEWLGYPSALLFGSGFIANLAVQQALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVE
GAMRQLKGAPEGAAMLATDGVFSMDGDVAPLRALSLVARMQQALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
LGKALGGYGAVVVGEEALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRARLVELIGAFRDGARKHGFELMA
SDTPIQPLLCGEEATVMAMSAALEHAGFMVGAIRPPTVPEGKARLRVTLSALHTQQQVQALIEAIVQARDVVSRQPLRAS
A
>Mature_400_residues
ARPDLHERISSLRKLRVAQERVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFEVVAALQDAAARDGAGATASHL
ICGHHTAHETLEREIAEWLGYPSALLFGSGFIANLAVQQALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVEG
AMRQLKGAPEGAAMLATDGVFSMDGDVAPLRALSLVARMQQALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVTL
GKALGGYGAVVVGEEALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRARLVELIGAFRDGARKHGFELMAS
DTPIQPLLCGEEATVMAMSAALEHAGFMVGAIRPPTVPEGKARLRVTLSALHTQQQVQALIEAIVQARDVVSRQPLRASA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=369, Percent_Identity=36.5853658536585, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI284448556, Length=347, Percent_Identity=37.463976945245, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI83977444, Length=355, Percent_Identity=32.3943661971831, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI83977440, Length=355, Percent_Identity=32.3943661971831, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI83977442, Length=355, Percent_Identity=32.3943661971831, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4758668, Length=365, Percent_Identity=27.3972602739726, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI4502025, Length=405, Percent_Identity=29.1358024691358, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI40316939, Length=405, Percent_Identity=29.1358024691358, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI119220554, Length=362, Percent_Identity=25.6906077348066, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI5454084, Length=250, Percent_Identity=22.4, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1786993, Length=354, Percent_Identity=43.5028248587571, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1790046, Length=352, Percent_Identity=35.2272727272727, Blast_Score=207, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI71994529, Length=368, Percent_Identity=36.4130434782609, Blast_Score=228, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI32566772, Length=360, Percent_Identity=27.7777777777778, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17560912, Length=385, Percent_Identity=26.4935064935065, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17560914, Length=385, Percent_Identity=26.4935064935065, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71982625, Length=285, Percent_Identity=24.2105263157895, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71982617, Length=285, Percent_Identity=24.2105263157895, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320438, Length=366, Percent_Identity=29.5081967213115, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320267, Length=372, Percent_Identity=22.8494623655914, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323954, Length=383, Percent_Identity=23.2375979112272, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24662918, Length=347, Percent_Identity=35.7348703170029, Blast_Score=221, Evalue=9e-58,
Organism=Drosophila melanogaster, GI17137420, Length=396, Percent_Identity=30.5555555555556, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI17136286, Length=381, Percent_Identity=26.246719160105, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24653280, Length=280, Percent_Identity=24.6428571428571, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24653276, Length=280, Percent_Identity=24.6428571428571, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24653278, Length=280, Percent_Identity=24.6428571428571, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_XANAC (Q8PQD8)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_640743.1
- HSSP:   P12998
- ProteinModelPortal:   Q8PQD8
- GeneID:   1154458
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0387
- NMPDR:   fig|190486.1.peg.387
- HOGENOM:   HBG649839
- OMA:   ATVMAMS
- ProtClustDB:   PRK05958
- BioCyc:   XAXO190486:XAC0387-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 43182; Mature: 43051

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 24-24 BINDING 137-137 BINDING 183-183 BINDING 357-357

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPDLHERISSLRKLRVAQERVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCCHHHHH
VVAALQDAAARDGAGATASHLICGHHTAHETLEREIAEWLGYPSALLFGSGFIANLAVQQ
HHHHHHHHHHCCCCCCHHHHEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHH
ALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVEGAMRQLKGAPEGAAMLATDG
HHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEECC
VFSMDGDVAPLRALSLVARMQQALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
EEECCCCHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCCHHCCCCHHHHHHHHHH
LGKALGGYGAVVVGEEALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRARL
HHHHHCCCCEEEECHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHH
VELIGAFRDGARKHGFELMASDTPIQPLLCGEEATVMAMSAALEHAGFMVGAIRPPTVPE
HHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHCCEEEECCCCCCCCC
GKARLRVTLSALHTQQQVQALIEAIVQARDVVSRQPLRASA
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARPDLHERISSLRKLRVAQERVRVRRQVGRRDGVRLEIDGRWLTGFCSNDYLGLSQQFE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCCHHHHH
VVAALQDAAARDGAGATASHLICGHHTAHETLEREIAEWLGYPSALLFGSGFIANLAVQQ
HHHHHHHHHHCCCCCCHHHHEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHH
ALLSEEDDVCVQDRLNHASLLDATRLAGCRLRRYPHLDVEGAMRQLKGAPEGAAMLATDG
HHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEECC
VFSMDGDVAPLRALSLVARMQQALFYVDDAHGVGVLGPQGRGCVADAGLGVAEVPLQLVT
EEECCCCHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCCHHCCCCHHHHHHHHHH
LGKALGGYGAVVVGEEALVRHLAETARPYIYTTALPPAQVAATLAAVRLARRDDWRRARL
HHHHHCCCCEEEECHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHH
VELIGAFRDGARKHGFELMASDTPIQPLLCGEEATVMAMSAALEHAGFMVGAIRPPTVPE
HHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHCCEEEECCCCCCCCC
GKARLRVTLSALHTQQQVQALIEAIVQARDVVSRQPLRASA
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217