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The map label for this gene is hsdR [H]

Identifier: 21228712

GI number: 21228712

Start: 3105788

End: 3106687

Strand: Reverse

Name: hsdR [H]

Synonym: MM_2610

Alternate gene names: 21228712

Gene position: 3106687-3105788 (Counterclockwise)

Preceding gene: 21228713

Following gene: 21228710

Centisome position: 75.84

GC content: 46.44

Gene sequence:

>900_bases
GTGGAAGCAAAGAGGAATATTTACACGGTTGGAGAGGGCATGCAGCAGGCGCTCATGTATGCTGAGATGCTGGACATTCC
TTTTGCATTCAGCTCGAATGGGGATGCCTTTTTGCTCCACGACAGGACAGGCACTCTTGGAAAGGTAGAAGAGGAAATTT
CTCTGGACAAATTTCCTTCCCCTGAATTTCTCTGGCGGAAATACTGCGAATGGAAGGGGCTTGACGAAGACAGGCAGAGG
ATCGTAACCCAGGACTATTTTTCCGATCGGCAGAAATCCCTGCGATATTATCAGCGTATAGCAATTAACCGGACAATAGA
GGCAATTGCAAAAGGGCAGGACAGAATCCTGCTGGTTATGGCAACAGGTACGGGAAAGACTCTCACGGCATTTCAGATCA
TCTGGCGCCTCTGGAAGGCAGGGGTGAAAAAAAGAATCCTTTTCCTGGCAGACAGAAACATCCTTGTTGACCAGACAAAA
ATTAACGATTTCAAGCATTTCGGGAAGGCAATGACAAAAATCAAGAACCGGGAGGCTGAAAAATCCTACGAAATTTACCT
GTCCCTCTATCAGGCTGTTTCAGGCACAGAAGAAGAGAAAAACATCTACAAACAGTTTTCCCCTGACTTTTTTGACCTTG
TGATTGTGGACGAATGCCACAGGGGAAGCGCGGCTGAAGATTCCGCATGGAGGGAAATACTCGAATATTTCTCTTCGGCA
ACGCAGATCGGGCTGACAGCTACCCCGAAAGAGACAAAAGACGTTTCCAATATCCATTACTTCGGAGAGCCCATCTACAC
ATATTCCCTGATATCCATTAAAAAATCTACGGAATCCCTTTCCTCGGCCTTCATAGAGCTTCAACCTCATTCAGGATGCG
CTTTCCTATATTGGGTTTGA

Upstream 100 bases:

>100_bases
CAGGGGAAGGATTATTGTTAAAGGTTCATCTGCGAGCAGAGGAAAACCAAAGAGAGCTGACTATATTCTTTCCTATAAAC
CCAACCTTCCTCTTGCAGTT

Downstream 100 bases:

>100_bases
GTGCGCTCTTCATTACCAGGTCAACCTTTATTCCTAAAAGGTCGCTCAAATAATTTTCCAGCTCAATATATTTCAGCAGT
CCTGGTTTCTTATCAAACTG

Product: Type I restriction enzyme

Products: NA

Alternate protein names: R.EcoEI [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MEAKRNIYTVGEGMQQALMYAEMLDIPFAFSSNGDAFLLHDRTGTLGKVEEEISLDKFPSPEFLWRKYCEWKGLDEDRQR
IVTQDYFSDRQKSLRYYQRIAINRTIEAIAKGQDRILLVMATGTGKTLTAFQIIWRLWKAGVKKRILFLADRNILVDQTK
INDFKHFGKAMTKIKNREAEKSYEIYLSLYQAVSGTEEEKNIYKQFSPDFFDLVIVDECHRGSAAEDSAWREILEYFSSA
TQIGLTATPKETKDVSNIHYFGEPIYTYSLISIKKSTESLSSAFIELQPHSGCAFLYWV

Sequences:

>Translated_299_residues
MEAKRNIYTVGEGMQQALMYAEMLDIPFAFSSNGDAFLLHDRTGTLGKVEEEISLDKFPSPEFLWRKYCEWKGLDEDRQR
IVTQDYFSDRQKSLRYYQRIAINRTIEAIAKGQDRILLVMATGTGKTLTAFQIIWRLWKAGVKKRILFLADRNILVDQTK
INDFKHFGKAMTKIKNREAEKSYEIYLSLYQAVSGTEEEKNIYKQFSPDFFDLVIVDECHRGSAAEDSAWREILEYFSSA
TQIGLTATPKETKDVSNIHYFGEPIYTYSLISIKKSTESLSSAFIELQPHSGCAFLYWV
>Mature_299_residues
MEAKRNIYTVGEGMQQALMYAEMLDIPFAFSSNGDAFLLHDRTGTLGKVEEEISLDKFPSPEFLWRKYCEWKGLDEDRQR
IVTQDYFSDRQKSLRYYQRIAINRTIEAIAKGQDRILLVMATGTGKTLTAFQIIWRLWKAGVKKRILFLADRNILVDQTK
INDFKHFGKAMTKIKNREAEKSYEIYLSLYQAVSGTEEEKNIYKQFSPDFFDLVIVDECHRGSAAEDSAWREILEYFSSA
TQIGLTATPKETKDVSNIHYFGEPIYTYSLISIKKSTESLSSAFIELQPHSGCAFLYWV

Specific function: The EcoEI enzyme recognizes 5'-GAGN(7)ATGC-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG4096

COG function: function code V; Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI226510991, Length=275, Percent_Identity=30.9090909090909, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR013670
- InterPro:   IPR007409 [H]

Pfam domain/function: PF08463 EcoEI_R_C; PF04313 HSDR_N; PF04851 ResIII [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 34589; Mature: 34589

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKRNIYTVGEGMQQALMYAEMLDIPFAFSSNGDAFLLHDRTGTLGKVEEEISLDKFPS
CCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCHHHHHHCCCCCCC
PEFLWRKYCEWKGLDEDRQRIVTQDYFSDRQKSLRYYQRIAINRTIEAIAKGQDRILLVM
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
ATGTGKTLTAFQIIWRLWKAGVKKRILFLADRNILVDQTKINDFKHFGKAMTKIKNREAE
ECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCEEEECHHHHHHHHHHHHHHHHHCCCHH
KSYEIYLSLYQAVSGTEEEKNIYKQFSPDFFDLVIVDECHRGSAAEDSAWREILEYFSSA
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
TQIGLTATPKETKDVSNIHYFGEPIYTYSLISIKKSTESLSSAFIELQPHSGCAFLYWV
HHCCEEECCCCCCCCCCEEECCCCEEEEEEEEEHHHHHHHHHHEEEEECCCCEEEEEEC
>Mature Secondary Structure
MEAKRNIYTVGEGMQQALMYAEMLDIPFAFSSNGDAFLLHDRTGTLGKVEEEISLDKFPS
CCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCHHHHHHCCCCCCC
PEFLWRKYCEWKGLDEDRQRIVTQDYFSDRQKSLRYYQRIAINRTIEAIAKGQDRILLVM
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
ATGTGKTLTAFQIIWRLWKAGVKKRILFLADRNILVDQTKINDFKHFGKAMTKIKNREAE
ECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCEEEECHHHHHHHHHHHHHHHHHCCCHH
KSYEIYLSLYQAVSGTEEEKNIYKQFSPDFFDLVIVDECHRGSAAEDSAWREILEYFSSA
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
TQIGLTATPKETKDVSNIHYFGEPIYTYSLISIKKSTESLSSAFIELQPHSGCAFLYWV
HHCCEEECCCCCCCCCCEEECCCCEEEEEEEEEHHHHHHHHHHEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]